(Circular) (Six-base) MAPSORT of: pFN21AA1384.seq Check: 8734 from: 1 to: 6589 pFN21AA1384 6589 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2557 2557 Size: 6589 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3932 1937 Size: 1995 4594 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2902 4652 6036 6304 1632 Size: 1270 1750 1384 268 1917 Fragments arranged by size: 1917 1750 1384 1270 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 5488 6465 1203 Size: 4285 977 1327 Fragments arranged by size: 4285 1327 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 5318 5318 Size: 6589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6589 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2510 1184 Size: 1326 5263 AlwNI CAG_nnn'CTG Cuts at: 2881 2941 3385 3842 4332 5904 2881 Size: 60 444 457 490 1572 3566 Fragments arranged by size: 3566 1572 490 457 444 60 ApaI G_GGCC'C Cuts at: 2245 2245 Size: 6589 ApaLI G'TGCA_C Cuts at: 3407 4646 5802 3407 Size: 1239 1156 4194 Fragments arranged by size: 4194 1239 1156 ApoI r'AATT_y Cuts at: 1493 2952 3178 3898 4124 4276 6385 1493 Size: 1459 226 720 226 152 2109 1697 Fragments arranged by size: 2109 1697 1459 720 226 226 152 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 2207 2315 2361 2420 3914 1870 Size: 73 264 108 46 59 1494 4545 Fragments arranged by size: 4545 1494 264 108 73 59 46 BamHI G'GATC_C Cuts at: 3919 4286 3919 Size: 367 6222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3910 5365 618 Size: 325 217 627 2123 1455 1842 Fragments arranged by size: 2123 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2245 2316 3502 3908 1391 Size: 854 71 1186 406 4072 Fragments arranged by size: 4072 1186 854 406 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3059 928 Size: 971 1160 4458 Fragments arranged by size: 4458 1160 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4780 4814 1903 Size: 34 2843 34 3678 Fragments arranged by size: 3678 2843 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4481 5691 4481 Size: 1210 5379 BclI T'GATC_A Cuts at: 1548 1548 Size: 6589 BfrBI ATG'CAT Cuts at: 3260 3260 Size: 6589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2156 2232 2920 Size: 107 122 71 631 605 484 76 688 Cuts at: 2920 5200 136 Size: 2280 1525 Fragments arranged by size: 2280 1525 688 631 605 484 122 107 76 71 BglII A'GATC_T Cuts at: 2544 6584 2544 Size: 4040 2549 BlpI GC'TnA_GC Cuts at: 4006 4006 Size: 6589 Bme1580I G_kGCm'C Cuts at: 1790 2245 3094 3411 4650 5806 1790 Size: 455 849 317 1239 1156 2573 Fragments arranged by size: 2573 1239 1156 849 455 317 BmgBI CAC'GTC Cuts at: 1318 2107 1318 Size: 789 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3476 4320 5278 Size: 416 53 83 399 24 2059 844 958 Cuts at: 5278 442 Size: 1753 Fragments arranged by size: 2059 1753 958 844 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 2618 2762 3763 5249 1239 Size: 937 442 144 1001 1486 2579 Fragments arranged by size: 2579 1486 1001 937 442 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4714 5579 5877 4714 Size: 865 298 5426 Fragments arranged by size: 5426 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2597 2750 5252 882 Size: 617 1098 153 2502 2219 Fragments arranged by size: 2502 2219 1098 617 153 BsaAI yAC'GTr Cuts at: 493 1204 3286 4380 493 Size: 711 2082 1094 2702 Fragments arranged by size: 2702 2082 1094 711 BsaBI GATnn'nnATC Cuts at: 6583 6583 Size: 6589 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2892 4396 4778 5413 Size: 53 83 186 936 1359 1504 382 635 Cuts at: 5413 275 Size: 1451 Fragments arranged by size: 1504 1451 1359 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3004 5022 5420 5694 5841 6252 3004 Size: 2018 398 274 147 411 3341 Fragments arranged by size: 3341 2018 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2012 2042 2462 2492 3441 Size: 30 165 30 609 30 420 30 949 Cuts at: 3441 3471 1178 Size: 30 4296 Fragments arranged by size: 4296 949 609 420 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 2483 2615 2675 1186 Size: 1138 159 132 60 5100 Fragments arranged by size: 5100 1138 159 132 60 BseYI C'CCAG_C Cuts at: 2042 3017 5792 6288 2042 Size: 975 2775 496 2343 Fragments arranged by size: 2775 2343 975 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 2480 2546 2573 3720 1163 Size: 573 312 432 66 27 1147 4032 Fragments arranged by size: 4032 1147 573 432 312 66 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2183 2752 4039 4800 5828 1540 Size: 275 186 182 569 1287 761 1028 2301 Fragments arranged by size: 2301 1287 1028 761 569 275 186 182 BsiHKAI G_wGCw'C Cuts at: 2421 2748 3411 4650 4735 5806 2421 Size: 327 663 1239 85 1071 3204 Fragments arranged by size: 3204 1239 1071 663 327 85 BsmI GAATG_Cn' Cuts at: 3629 4100 4193 3629 Size: 471 93 6025 Fragments arranged by size: 6025 471 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2245 2316 2421 2748 3094 3411 3502 Size: 399 455 71 105 327 346 317 91 Cuts at: 3502 3908 4650 4735 5806 1391 Size: 406 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 455 406 399 346 327 317 105 91 85 71 BspEI T'CCGG_A Cuts at: 6252 6252 Size: 6589 BspHI T'CATG_A Cuts at: 2784 4478 6208 2784 Size: 1694 1730 3165 Fragments arranged by size: 3165 1730 1694 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 2085 2557 3944 844 Size: 1223 18 472 1387 3489 Fragments arranged by size: 3489 1387 1223 472 18 BsrBI CCG'CTC Cuts at: 1394 1823 2833 4476 1394 Size: 429 1010 1643 3507 Fragments arranged by size: 3507 1643 1010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 5084 5258 65 Size: 1183 889 2947 174 1396 Fragments arranged by size: 2947 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3009 5233 5420 1700 Size: 110 1199 2224 187 2869 Fragments arranged by size: 2869 2224 1199 187 110 BsrGI T'GTAC_A Cuts at: 96 3439 96 Size: 3343 3246 BssHII G'CGCG_C Cuts at: 1925 2668 1925 Size: 743 5846 BssSI C'ACGA_G Cuts at: 4649 5661 4649 Size: 1012 5577 BstAPI GCAn_nnn'nTGC Cuts at: 794 3842 794 Size: 3048 3541 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 2509 3641 1184 Size: 1325 1132 4132 Fragments arranged by size: 4132 1325 1132 BstXI CCAn_nnnn'nTGG Cuts at: 2614 3025 2614 Size: 411 6178 BstYI r'GATC_y Cuts at: 1972 2012 2490 2544 3919 4286 4671 4688 6140 Size: 40 478 54 1375 367 385 17 1452 Cuts at: 6140 6226 6238 6584 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1375 478 385 367 346 86 54 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2697 3461 513 Size: 552 1632 764 3641 Fragments arranged by size: 3641 1632 764 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5850 Fragments arranged by size: 5850 683 56 BtsI GCAGTG_nn' Cuts at: 2926 3097 3411 3708 4105 4899 4919 2926 Size: 171 314 297 397 794 20 4596 Fragments arranged by size: 4596 794 397 314 297 171 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 3893 3893 Size: 6589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5596 809 Size: 507 4280 1802 Fragments arranged by size: 4280 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2224 2436 2616 Size: 54 1425 50 275 18 394 212 180 Cuts at: 2616 2943 4036 4925 6554 8 Size: 327 1093 889 1629 43 Fragments arranged by size: 1629 1425 1093 889 394 327 275 212 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4036 1537 Size: 275 2224 4090 Fragments arranged by size: 4090 2224 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2192 2723 3238 4519 1353 Size: 839 531 515 1281 3423 Fragments arranged by size: 3423 1281 839 531 515 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5176 5550 5696 387 Size: 1064 138 3587 374 146 1280 Fragments arranged by size: 3587 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2618 2762 2902 3763 4652 5249 Size: 393 544 442 144 140 861 889 597 Cuts at: 5249 6036 6304 1239 Size: 787 268 1524 Fragments arranged by size: 1524 889 861 787 597 544 442 393 268 144 140 EcoNI CCTnn'n_nnAGG Cuts at: 2633 2633 Size: 6589 EcoO109I rG'GnC_Cy Cuts at: 2025 2241 2242 3753 3839 2025 Size: 216 1 1511 86 4775 Fragments arranged by size: 4775 1511 216 86 1 EcoRI G'AATT_C Cuts at: 2952 3898 4276 2952 Size: 946 378 5265 Fragments arranged by size: 5265 946 378 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 2717 2794 3361 5736 1678 Size: 1039 77 567 2375 2531 Fragments arranged by size: 2531 2375 1039 567 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2462 2494 2531 Size: 32 135 32 440 32 418 32 37 Cuts at: 2531 2563 3441 3473 5230 5262 5304 5336 1373 Size: 32 878 32 1757 32 42 32 2626 Fragments arranged by size: 2626 1757 878 440 418 135 42 37 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3933 4179 669 Size: 988 281 1995 246 3079 Fragments arranged by size: 3079 1995 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6589 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 3570 4188 3570 Size: 618 5971 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2685 2965 3249 5703 5887 1305 Size: 1380 280 284 2454 184 2007 Fragments arranged by size: 2454 2007 1380 284 280 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 2945 10 Size: 54 1425 343 786 327 3654 Fragments arranged by size: 3654 1425 786 343 327 54 MslI CAynn'nnrTG Cuts at: 518 1166 3074 3606 4547 4906 5065 518 Size: 648 1908 532 941 359 159 2042 Fragments arranged by size: 2042 1908 941 648 532 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 3741 4005 4682 5830 6075 1606 Size: 489 1646 264 677 1148 245 2120 Fragments arranged by size: 2120 1646 1148 677 489 264 245 NcoI C'CATG_G Cuts at: 513 1065 3461 513 Size: 552 2396 3641 Fragments arranged by size: 3641 2396 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NsiI A_TGCA'T Cuts at: 3262 3262 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 2767 3264 3947 5492 1132 Size: 97 1538 497 683 1545 2229 Fragments arranged by size: 2229 1545 1538 683 497 97 PasI CC'CwG_GG Cuts at: 1348 2122 1348 Size: 774 5815 PciI A'CATG_T Cuts at: 5488 5488 Size: 6589 PflMI CCAn_nnn'nTGG Cuts at: 1108 2845 1108 Size: 1737 4852 PfoI T'CCnGG_A Cuts at: 2998 2998 Size: 6589 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2025 2025 Size: 6589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 4159 4159 Size: 6589 PspOMI G'GGCC_C Cuts at: 2241 2241 Size: 6589 PspXI vC'TCGA_Gb Cuts at: 2420 2420 Size: 6589 PstI C_TGCA'G Cuts at: 830 2388 2933 3941 830 Size: 1558 545 1008 3478 Fragments arranged by size: 3478 1558 1008 545 PvuI CG_AT'CG Cuts at: 2001 2183 2001 Size: 182 6407 PvuII CAG'CTG Cuts at: 2095 3741 2095 Size: 1646 4943 RsrII CG'GwC_CG Cuts at: 3007 3007 Size: 6589 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SapI GCTCTTCn'nnn_ Cuts at: 2192 2723 3238 2192 Size: 531 515 5543 Fragments arranged by size: 5543 531 515 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 2174 4837 6549 2174 Size: 2663 1712 2214 Fragments arranged by size: 2663 2214 1712 SexAI A'CCwGG_T Cuts at: 2376 2628 2376 Size: 252 6337 SfcI C'TryA_G Cuts at: 826 1045 2384 2929 3736 3937 5072 5753 5944 Size: 219 1339 545 807 201 1135 681 191 Cuts at: 5944 826 Size: 1471 Fragments arranged by size: 1471 1339 1135 807 681 545 219 201 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2156 2232 2156 Size: 76 6513 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 2317 2363 3916 1872 Size: 445 46 1553 4545 Fragments arranged by size: 4545 1553 445 46 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 4693 5594 5856 820 Size: 197 926 477 2273 901 262 1553 Fragments arranged by size: 2273 1553 926 901 477 262 197 SnaBI TAC'GTA Cuts at: 493 3286 4380 493 Size: 2793 1094 2702 Fragments arranged by size: 2793 2702 1094 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 2767 3947 1132 Size: 1635 1180 3774 Fragments arranged by size: 3774 1635 1180 SspI AAT'ATT Cuts at: 5 52 4513 5 Size: 47 4461 2081 Fragments arranged by size: 4461 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6589 StyI C'CwwG_G Cuts at: 513 1065 2247 3461 4028 513 Size: 552 1182 1214 567 3074 Fragments arranged by size: 3074 1214 1182 567 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2470 3125 3244 4616 4633 4786 4971 Size: 1255 890 655 119 1372 17 153 185 Cuts at: 4971 5416 325 Size: 445 1498 Fragments arranged by size: 1498 1372 1255 890 655 445 185 153 119 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 3173 3439 Size: 274 80 33 51 1443 195 1001 266 Cuts at: 3439 4835 6547 96 Size: 1396 1712 138 Fragments arranged by size: 1712 1443 1396 1001 274 266 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6549 6581 6549 Size: 32 6557 Tth111I GACn'n_nGTC Cuts at: 1316 2449 2867 1316 Size: 1133 418 5038 Fragments arranged by size: 5038 1133 418 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2374 2626 2374 Size: 252 6337 XhoI C'TCGA_G Cuts at: 1943 2420 1943 Size: 477 6112 XmaI C'CCGG_G Cuts at: 1870 2315 2361 3914 1870 Size: 445 46 1553 4545 Fragments arranged by size: 4545 1553 445 46 XmnI GAAnn'nnTTC Cuts at: 3593 4718 3593 Size: 1125 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI SacI SacII SanDI SfoI SrfI SwaI