Restriction map for
pFN21AA1384
(Linear) (Six-base) MAPSORT of: KIAA1384.seq Check: 2249 from: 1 to: 1884
KIAA1384.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 552 1884
Size: 552 1332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 1884
Size: 897 987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 505 1884
Size: 505 1379
AlwNI CAG_nnn'CTG
Cuts at: 0 876 936 1380 1837 1884
Size: 876 60 444 457 47
Fragments arranged by size:
876 457 444 60 47
ApaI G_GGCC'C
Cuts at: 0 240 1884
Size: 240 1644
ApaLI G'TGCA_C
Cuts at: 0 1402 1884
Size: 1402 482
ApoI r'AATT_y
Cuts at: 0 947 1173 1884
Size: 947 226 711
Fragments arranged by size:
947 711 226
AvaI C'yCGr_G
Cuts at: 0 202 310 356 415 1884
Size: 202 108 46 59 1469
Fragments arranged by size:
1469 202 108 59 46
BanII G_rGCy'C
Cuts at: 0 240 311 1497 1884
Size: 240 71 1186 387
Fragments arranged by size:
1186 387 240 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 1054 1884
Size: 1054 830
BfrBI ATG'CAT
Cuts at: 0 1255 1884
Size: 1255 629
BglI GCCn_nnn'nGGC
Cuts at: 0 151 227 915 1884
Size: 151 76 688 969
Fragments arranged by size:
969 688 151 76
BglII A'GATC_T
Cuts at: 0 539 1884
Size: 539 1345
Bme1580I G_kGCm'C
Cuts at: 0 240 1089 1406 1884
Size: 240 849 317 478
Fragments arranged by size:
849 478 317 240
BmgBI CAC'GTC
Cuts at: 0 102 1884
Size: 102 1782
BmrI ACTGGGnnnn_n'
Cuts at: 0 1471 1884
Size: 1471 413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 613 757 1758 1884
Size: 171 442 144 1001 126
Fragments arranged by size:
1001 442 171 144 126
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 745 1884
Size: 592 153 1139
Fragments arranged by size:
1139 592 153
BsaAI yAC'GTr
Cuts at: 0 1281 1884
Size: 1281 603
BsaHI Gr'CG_yC
Cuts at: 0 887 1884
Size: 887 997
BsaWI w'CCGG_w
Cuts at: 0 999 1884
Size: 999 885
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 457 487 1436 1466 1884
Size: 7 30 420 30 949 30 418
Fragments arranged by size:
949 420 418 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 478 610 670 1884
Size: 319 159 132 60 1214
Fragments arranged by size:
1214 319 159 132 60
BseYI C'CCAG_C
Cuts at: 0 37 1012 1884
Size: 37 975 872
Fragments arranged by size:
975 872 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 475 541 568 1715 1884
Size: 43 432 66 27 1147 169
Fragments arranged by size:
1147 432 169 66 43 27
BsiEI CG_ry'CG
Cuts at: 0 178 747 1884
Size: 178 569 1137
Fragments arranged by size:
1137 569 178
BsiHKAI G_wGCw'C
Cuts at: 0 416 743 1406 1884
Size: 416 327 663 478
Fragments arranged by size:
663 478 416 327
BsmI GAATG_Cn'
Cuts at: 0 1624 1884
Size: 1624 260
Bsp1286I G_dGCh'C
Cuts at: 0 240 311 416 743 1089 1406 1497 1884
Size: 240 71 105 327 346 317 91 387
Fragments arranged by size:
387 346 327 317 240 105 91 71
BspHI T'CATG_A
Cuts at: 0 779 1884
Size: 779 1105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 80 552 1884
Size: 62 18 472 1332
Fragments arranged by size:
1332 472 62 18
BsrBI CCG'CTC
Cuts at: 0 828 1884
Size: 828 1056
BsrDI GCAATG_nn'
Cuts at: 0 132 1884
Size: 132 1752
BsrFI r'CCGG_y
Cuts at: 0 1004 1884
Size: 1004 880
BsrGI T'GTAC_A
Cuts at: 0 1434 1884
Size: 1434 450
BssHII G'CGCG_C
Cuts at: 0 663 1884
Size: 663 1221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1837 1884
Size: 1837 47
BstEII G'GTnAC_C
Cuts at: 0 504 1636 1884
Size: 504 1132 248
Fragments arranged by size:
1132 504 248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 609 1020 1884
Size: 609 411 864
Fragments arranged by size:
864 609 411
BstYI r'GATC_y
Cuts at: 0 7 485 539 1884
Size: 7 478 54 1345
Fragments arranged by size:
1345 478 54 7
BtgI C'CryG_G
Cuts at: 0 692 1456 1884
Size: 692 764 428
Fragments arranged by size:
764 692 428
BtsI GCAGTG_nn'
Cuts at: 0 921 1092 1406 1703 1884
Size: 921 171 314 297 181
Fragments arranged by size:
921 314 297 181 171
EaeI y'GGCC_r
Cuts at: 0 219 431 611 938 1884
Size: 219 212 180 327 946
Fragments arranged by size:
946 327 219 212 180
EarI CTCTTCn'nnn_
Cuts at: 0 187 718 1233 1884
Size: 187 531 515 651
Fragments arranged by size:
651 531 515 187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 613 757 897 1758 1884
Size: 171 442 144 140 861 126
Fragments arranged by size:
861 442 171 144 140 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 1884
Size: 628 1256
EcoO109I rG'GnC_Cy
Cuts at: 0 20 236 237 1748 1834 1884
Size: 20 216 1 1511 86 50
Fragments arranged by size:
1511 216 86 50 20 1
EcoRI G'AATT_C
Cuts at: 0 947 1884
Size: 947 937
HaeII r_GCGC'y
Cuts at: 0 712 789 1356 1884
Size: 712 77 567 528
Fragments arranged by size:
712 567 528 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 457 489 526 558 1436 1468
Size: 7 32 418 32 37 32 878 32
Cuts at: 1468 1884
Size: 416
Fragments arranged by size:
878 418 416 37 32 32 32 32
7
MfeI C'AATT_G
Cuts at: 0 1565 1884
Size: 1565 319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 960 1244 1884
Size: 680 280 284 640
Fragments arranged by size:
680 640 284 280
MscI TGG'CCA
Cuts at: 0 613 940 1884
Size: 613 327 944
Fragments arranged by size:
944 613 327
MslI CAynn'nnrTG
Cuts at: 0 1069 1601 1884
Size: 1069 532 283
Fragments arranged by size:
1069 532 283
MspA1I CmG'CkG
Cuts at: 0 90 1736 1884
Size: 90 1646 148
Fragments arranged by size:
1646 148 90
NcoI C'CATG_G
Cuts at: 0 1456 1884
Size: 1456 428
NsiI A_TGCA'T
Cuts at: 0 1257 1884
Size: 1257 627
NspI r_CATG'y
Cuts at: 0 762 1259 1884
Size: 762 497 625
Fragments arranged by size:
762 625 497
PasI CC'CwG_GG
Cuts at: 0 117 1884
Size: 117 1767
PflMI CCAn_nnn'nTGG
Cuts at: 0 840 1884
Size: 840 1044
PfoI T'CCnGG_A
Cuts at: 0 993 1884
Size: 993 891
PpuMI rG'GwC_Cy
Cuts at: 0 20 1884
Size: 20 1864
PspOMI G'GGCC_C
Cuts at: 0 236 1884
Size: 236 1648
PspXI vC'TCGA_Gb
Cuts at: 0 415 1884
Size: 415 1469
PstI C_TGCA'G
Cuts at: 0 383 928 1884
Size: 383 545 956
Fragments arranged by size:
956 545 383
PvuI CG_AT'CG
Cuts at: 0 178 1884
Size: 178 1706
PvuII CAG'CTG
Cuts at: 0 90 1736 1884
Size: 90 1646 148
Fragments arranged by size:
1646 148 90
RsrII CG'GwC_CG
Cuts at: 0 1002 1884
Size: 1002 882
SapI GCTCTTCn'nnn_
Cuts at: 0 187 718 1233 1884
Size: 187 531 515 651
Fragments arranged by size:
651 531 515 187
ScaI AGT'ACT
Cuts at: 0 169 1884
Size: 169 1715
SexAI A'CCwGG_T
Cuts at: 0 371 623 1884
Size: 371 252 1261
Fragments arranged by size:
1261 371 252
SfcI C'TryA_G
Cuts at: 0 379 924 1731 1884
Size: 379 545 807 153
Fragments arranged by size:
807 545 379 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 151 227 1884
Size: 151 76 1657
Fragments arranged by size:
1657 151 76
SmaI CCC'GGG
Cuts at: 0 312 358 1884
Size: 312 46 1526
Fragments arranged by size:
1526 312 46
SmlI C'TyrA_G
Cuts at: 0 415 1884
Size: 415 1469
SnaBI TAC'GTA
Cuts at: 0 1281 1884
Size: 1281 603
SphI G_CATG'C
Cuts at: 0 762 1884
Size: 762 1122
StyI C'CwwG_G
Cuts at: 0 242 1456 1884
Size: 242 1214 428
Fragments arranged by size:
1214 428 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 465 1120 1239 1884
Size: 465 655 119 645
Fragments arranged by size:
655 645 465 119
TatI w'GTAC_w
Cuts at: 0 167 1168 1434 1884
Size: 167 1001 266 450
Fragments arranged by size:
1001 450 266 167
Tth111I GACn'n_nGTC
Cuts at: 0 444 862 1884
Size: 444 418 1022
Fragments arranged by size:
1022 444 418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 621 1884
Size: 369 252 1263
Fragments arranged by size:
1263 369 252
XhoI C'TCGA_G
Cuts at: 0 415 1884
Size: 415 1469
XmaI C'CCGG_G
Cuts at: 0 310 356 1884
Size: 310 46 1528
Fragments arranged by size:
1528 310 46
XmnI GAAnn'nnTTC
Cuts at: 0 1588 1884
Size: 1588 296
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI
BanII BbsI BfrBI BglI BglII Bme1580I BmgBI BmrI
BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI
BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I MfeI MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PflMI PfoI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SapI ScaI SexAI SfcI
SfiI SmaI SmlI SnaBI SphI StyI TaqII TatI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BlpI
BmtI BplI Bpu10I BpuEI BsaBI BsiWI BsmBI BspEI
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PmlI PpiI PshAI PsiI PsrI SacI
SacII SalI SanDI SbfI SfoI SgfI SgrAI SpeI
SrfI SspI StuI SwaI TstI XbaI ZraI