(Circular) (Six-base) MAPSORT of: pFN21AA1372.seq Check: 6233 from: 1 to: 6952 pFN21AA1372 6952 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2241 4205 2241 Size: 1964 4988 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3678 4762 278 Size: 53 83 186 936 2142 1084 2468 Fragments arranged by size: 2468 2142 1084 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4295 1937 Size: 2358 4594 Acc65I G'GTAC_C Cuts at: 3867 4273 3867 Size: 406 6546 AclI AA'CG_TT Cuts at: 1563 5079 5452 1563 Size: 3516 373 3063 Fragments arranged by size: 3516 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2549 2575 2792 5015 6399 6667 1632 Size: 455 462 26 217 2223 1384 268 1917 Fragments arranged by size: 2223 1917 1384 462 455 268 217 26 AfeI AGC'GCT Cuts at: 3011 3011 Size: 6952 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6755 AflIII A'CryG_T Cuts at: 1203 3452 5851 6828 1203 Size: 2249 2399 977 1327 Fragments arranged by size: 2399 2249 1327 977 AgeI A'CCGG_T Cuts at: 5783 5783 Size: 6952 AhdI GACnn_n'nnGTC Cuts at: 2017 5681 2017 Size: 3664 3288 AleI CACnn'nnGTG Cuts at: 1166 3887 1166 Size: 2721 4231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6952 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2696 2728 2696 Size: 32 6920 AlwNI CAG_nnn'CTG Cuts at: 2434 2635 4695 6267 2434 Size: 201 2060 1572 3119 Fragments arranged by size: 3119 2060 1572 201 ApaI G_GGCC'C Cuts at: 3990 3990 Size: 6952 ApaLI G'TGCA_C Cuts at: 3090 3752 5009 6165 3090 Size: 662 1257 1156 3877 Fragments arranged by size: 3877 1257 1156 662 ApoI r'AATT_y Cuts at: 1493 4261 4487 4639 6748 1493 Size: 2768 226 152 2109 1697 Fragments arranged by size: 2768 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5506 160 Size: 872 4474 1606 Fragments arranged by size: 4474 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 AvaI C'yCGr_G Cuts at: 1870 1943 2290 2350 2861 2961 3066 3509 3854 Size: 73 347 60 511 100 105 443 345 Cuts at: 3854 3885 3989 4277 1870 Size: 31 104 288 4545 Fragments arranged by size: 4545 511 443 347 345 288 105 104 100 73 60 31 BamHI G'GATC_C Cuts at: 4282 4649 4282 Size: 367 6585 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2744 3654 3867 4273 5728 Size: 325 217 627 957 910 213 406 1455 Cuts at: 5728 618 Size: 1842 Fragments arranged by size: 1842 1455 957 910 627 406 325 217 213 BanII G_rGCy'C Cuts at: 1391 2871 3035 3470 3855 3990 4007 4029 4271 Size: 1480 164 435 385 135 17 22 242 Cuts at: 4271 1391 Size: 4072 Fragments arranged by size: 4072 1480 435 385 242 164 135 22 17 BbeI G_GCGC'C Cuts at: 3658 3658 Size: 6952 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3060 3402 3759 4176 4240 928 Size: 971 1161 342 357 417 64 3640 Fragments arranged by size: 3640 1161 971 417 357 342 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2086 2120 3533 3567 5143 5177 1903 Size: 34 149 34 1413 34 1576 34 3678 Fragments arranged by size: 3678 1576 1413 149 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4844 6054 4844 Size: 1210 5742 BclI T'GATC_A Cuts at: 1548 1548 Size: 6952 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3030 5563 136 Size: 107 122 71 631 605 1358 2533 1525 Fragments arranged by size: 2533 1525 1358 631 605 122 107 71 BglII A'GATC_T Cuts at: 6947 6947 Size: 6952 BlpI GC'TnA_GC Cuts at: 3378 3587 4369 3378 Size: 209 782 5961 Fragments arranged by size: 5961 782 209 Bme1580I G_kGCm'C Cuts at: 1790 2816 3094 3756 3990 5013 6169 1790 Size: 1026 278 662 234 1023 1156 2573 Fragments arranged by size: 2573 1156 1026 1023 662 278 234 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6952 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2023 4159 4683 Size: 416 53 83 399 24 606 2136 524 Cuts at: 4683 5641 442 Size: 958 1753 Fragments arranged by size: 2136 1753 958 606 524 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6952 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2641 4021 2641 Size: 1380 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 3780 4179 5612 1239 Size: 1088 1453 399 1433 2579 Fragments arranged by size: 2579 1453 1433 1088 399 Bpu10I CC'TnA_GC Cuts at: 3006 3006 Size: 6952 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 2819 5077 5942 6240 2171 Size: 648 2258 865 298 2883 Fragments arranged by size: 2883 2258 865 648 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2528 5615 882 Size: 617 1029 3087 2219 Fragments arranged by size: 3087 2219 1029 617 BsaAI yAC'GTr Cuts at: 493 1204 4743 493 Size: 711 3539 2702 Fragments arranged by size: 3539 2702 711 BsaBI GATnn'nnATC Cuts at: 6946 6946 Size: 6952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2582 2726 3655 3675 Size: 53 83 186 936 1049 144 929 20 Cuts at: 3675 4759 5141 5776 275 Size: 1084 382 635 1451 Fragments arranged by size: 1451 1084 1049 936 929 635 382 186 144 83 53 20 BsaWI w'CCGG_w Cuts at: 2343 4017 5385 5783 6057 6204 6615 2343 Size: 1674 1368 398 274 147 411 2680 Fragments arranged by size: 2680 1674 1368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2726 2942 2972 3911 Size: 30 165 30 1293 30 216 30 939 Cuts at: 3911 3941 1178 Size: 30 4189 Fragments arranged by size: 4189 1293 939 216 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2816 2879 2947 2981 2991 3266 3296 Size: 1042 588 63 68 34 10 275 30 Cuts at: 3296 3299 3338 3341 1186 Size: 3 39 3 4797 Fragments arranged by size: 4797 1042 588 275 68 63 39 34 30 10 3 3 BseYI C'CCAG_C Cuts at: 2434 2748 6155 6651 2434 Size: 314 3407 496 2735 Fragments arranged by size: 3407 2735 496 314 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 4019 4113 1163 Size: 573 1041 1242 94 4002 Fragments arranged by size: 4002 1242 1041 573 94 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4402 5163 6191 1540 Size: 275 186 2401 761 1028 2301 Fragments arranged by size: 2401 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2187 2607 3094 3756 5013 5098 6169 2187 Size: 420 487 662 1257 85 1071 2970 Fragments arranged by size: 2970 1257 1071 662 487 420 85 BsmI GAATG_Cn' Cuts at: 4463 4556 4463 Size: 93 6859 BsmBI CGTCTCn'nnnn_ Cuts at: 2959 3682 2959 Size: 723 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2607 2816 2871 3035 3094 3470 Size: 399 397 420 209 55 164 59 376 Cuts at: 3470 3756 3855 3990 4007 4029 4271 5013 5098 Size: 286 99 135 17 22 242 742 85 Cuts at: 5098 6169 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 420 399 397 376 286 242 209 164 135 99 85 59 55 22 17 BspEI T'CCGG_A Cuts at: 4017 6615 4017 Size: 2598 4354 BspHI T'CATG_A Cuts at: 4841 6571 4841 Size: 1730 5222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 2787 3648 4205 4307 844 Size: 1397 546 861 557 102 3489 Fragments arranged by size: 3489 1397 861 557 546 102 BsrBI CCG'CTC Cuts at: 1394 1823 3410 3448 3474 4839 1394 Size: 429 1587 38 26 1365 3507 Fragments arranged by size: 3507 1587 1365 429 38 26 BsrDI GCAATG_nn' Cuts at: 65 1248 2055 4228 5447 5621 65 Size: 1183 807 2173 1219 174 1396 Fragments arranged by size: 2173 1396 1219 1183 807 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3966 5596 5783 1700 Size: 110 2156 1630 187 2869 Fragments arranged by size: 2869 2156 1630 187 110 BsrGI T'GTAC_A Cuts at: 96 3168 96 Size: 3072 3880 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6952 BssSI C'ACGA_G Cuts at: 5012 6024 5012 Size: 1012 5940 BstAPI GCAn_nnn'nTGC Cuts at: 794 2544 794 Size: 1750 5202 BstBI TT'CG_AA Cuts at: 3906 5756 3906 Size: 1850 5102 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6952 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2305 Size: 6952 BstYI r'GATC_y Cuts at: 1972 2362 2820 2928 3663 4282 4649 5034 5051 Size: 390 458 108 735 619 367 385 17 Cuts at: 5051 6503 6589 6601 6947 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 735 619 458 390 385 367 346 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3939 3939 Size: 6952 BtgI C'CryG_G Cuts at: 513 1065 2256 3718 513 Size: 552 1191 1462 3747 Fragments arranged by size: 3747 1462 1191 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6213 Fragments arranged by size: 6213 683 56 BtsI GCAGTG_nn' Cuts at: 2060 2329 2657 3133 3617 3748 4468 5262 5282 Size: 269 328 476 484 131 720 794 20 Cuts at: 5282 2060 Size: 3730 Fragments arranged by size: 3730 794 720 484 476 328 269 131 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2874 2909 529 Size: 35 2310 35 4572 Fragments arranged by size: 4572 2310 35 35 DraI TTT'AAA Cuts at: 4256 4256 Size: 6952 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5959 809 Size: 507 4643 1802 Fragments arranged by size: 4643 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2496 3715 3721 Size: 54 1425 50 275 18 666 1219 6 Cuts at: 3721 4399 5288 6917 8 Size: 678 889 1629 43 Fragments arranged by size: 1629 1425 1219 889 678 666 275 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 4399 1537 Size: 275 2587 4090 Fragments arranged by size: 4090 2587 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2456 2793 3330 3339 3904 4012 4882 1353 Size: 1103 337 537 9 565 108 870 3423 Fragments arranged by size: 3423 1103 870 565 537 337 108 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2333 2847 3686 5539 5913 6059 Size: 1064 138 744 514 839 1853 374 146 Cuts at: 6059 387 Size: 1280 Fragments arranged by size: 1853 1280 1064 839 744 514 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2327 2549 2575 2792 3780 4179 Size: 393 455 240 222 26 217 988 399 Cuts at: 4179 5015 5612 6399 6667 1239 Size: 836 597 787 268 1524 Fragments arranged by size: 1524 988 836 787 597 455 399 393 268 240 222 217 26 EcoNI CCTnn'n_nnAGG Cuts at: 4037 4037 Size: 6952 EcoO109I rG'GnC_Cy Cuts at: 2071 2395 3113 3414 3986 2071 Size: 324 718 301 572 5037 Fragments arranged by size: 5037 718 572 324 301 EcoRI G'AATT_C Cuts at: 4261 4639 4261 Size: 378 6574 FspI TGC'GCA Cuts at: 3644 5458 3644 Size: 1814 5138 HaeII r_GCGC'y Cuts at: 1678 3013 3658 3810 6099 1678 Size: 1335 645 152 2289 2531 Fragments arranged by size: 2531 2289 1335 645 152 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2273 2305 2908 Size: 32 135 32 431 32 238 32 603 Cuts at: 2908 2920 2940 2952 3662 3694 4175 4207 5593 Size: 12 20 12 710 32 481 32 1386 Cuts at: 5593 5625 5667 5699 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1386 710 603 481 431 238 135 42 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2554 3493 4296 4542 669 Size: 988 281 616 939 803 246 3079 Fragments arranged by size: 3079 988 939 803 616 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6952 HpaI GTT'AAC Cuts at: 3493 4542 3493 Size: 1049 5903 KasI G'GCGC_C Cuts at: 3654 3654 Size: 6952 KpnI G_GTAC'C Cuts at: 3871 4277 3871 Size: 406 6546 MfeI C'AATT_G Cuts at: 4551 4551 Size: 6952 MluI A'CGCG_T Cuts at: 6828 6828 Size: 6952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6066 6250 1305 Size: 4761 184 2007 Fragments arranged by size: 4761 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 3723 10 Size: 54 1425 343 666 1225 3239 Fragments arranged by size: 3239 1425 1225 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3423 3887 4910 5269 5428 518 Size: 648 2257 464 1023 359 159 2042 Fragments arranged by size: 2257 2042 1023 648 464 359 159 MspA1I CmG'CkG Cuts at: 1606 2301 2314 2431 2683 3973 4368 5045 6193 Size: 695 13 117 252 1290 395 677 1148 Cuts at: 6193 6438 1606 Size: 245 2120 Fragments arranged by size: 2120 1290 1148 695 677 395 252 245 117 13 NarI GG'CG_CC Cuts at: 3655 3655 Size: 6952 NcoI C'CATG_G Cuts at: 513 1065 2256 513 Size: 552 1191 5209 Fragments arranged by size: 5209 1191 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6952 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6952 NotI GC'GGCC_GC Cuts at: 4399 4399 Size: 6952 NspI r_CATG'y Cuts at: 1132 1229 2197 2547 3456 4310 5855 1132 Size: 97 968 350 909 854 1545 2229 Fragments arranged by size: 2229 1545 968 909 854 350 97 PasI CC'CwG_GG Cuts at: 1348 2029 3978 1348 Size: 681 1949 4322 Fragments arranged by size: 4322 1949 681 PciI A'CATG_T Cuts at: 3452 5851 3452 Size: 2399 4553 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 3710 4243 1108 Size: 1227 1375 533 3817 Fragments arranged by size: 3817 1375 1227 533 PfoI T'CCnGG_A Cuts at: 2358 2720 3480 3666 2358 Size: 362 760 186 5644 Fragments arranged by size: 5644 760 362 186 PmeI GTTT'AAAC Cuts at: 4256 4256 Size: 6952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2696 2728 5021 5053 6534 6566 2696 Size: 32 2293 32 1481 32 3082 Fragments arranged by size: 3082 2293 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 3113 3414 2395 Size: 718 301 5933 Fragments arranged by size: 5933 718 301 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6952 PsiI TTA'TAA Cuts at: 4522 4522 Size: 6952 PspOMI G'GGCC_C Cuts at: 3986 3986 Size: 6952 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3851 3883 3851 Size: 32 6920 PstI C_TGCA'G Cuts at: 830 2652 3564 3743 4216 4304 830 Size: 1822 912 179 473 88 3478 Fragments arranged by size: 3478 1822 912 473 179 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6952 PvuII CAG'CTG Cuts at: 2301 2314 2431 2301 Size: 13 117 6822 Fragments arranged by size: 6822 117 13 SalI G'TCGA_C Cuts at: 1936 4294 1936 Size: 2358 4594 SanDI GG'GwC_CC Cuts at: 3414 3414 Size: 6952 SapI GCTCTTCn'nnn_ Cuts at: 2456 4012 2456 Size: 1556 5396 SbfI CC_TGCA'GG Cuts at: 3564 4216 4304 3564 Size: 652 88 6212 Fragments arranged by size: 6212 652 88 ScaI AGT'ACT Cuts at: 5200 6912 5200 Size: 1712 5240 SexAI A'CCwGG_T Cuts at: 3486 3486 Size: 6952 SfcI C'TryA_G Cuts at: 826 1045 2648 3560 3739 4082 4212 4300 5435 Size: 219 1603 912 179 343 130 88 1135 Cuts at: 5435 6116 6307 826 Size: 681 191 1471 Fragments arranged by size: 1603 1471 1135 912 681 343 219 191 179 130 88 SfoI GGC'GCC Cuts at: 3656 3656 Size: 6952 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6952 SmaI CCC'GGG Cuts at: 1872 2352 2963 3068 4279 1872 Size: 480 611 105 1211 4545 Fragments arranged by size: 4545 1211 611 480 105 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 2834 5056 5957 6219 820 Size: 197 926 243 648 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 648 262 243 197 SnaBI TAC'GTA Cuts at: 493 4743 493 Size: 4250 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6952 SphI G_CATG'C Cuts at: 1132 4310 1132 Size: 3178 3774 SspI AAT'ATT Cuts at: 5 52 2041 4876 5 Size: 47 1989 2835 2081 Fragments arranged by size: 2835 2081 1989 47 StuI AGG'CCT Cuts at: 1517 2570 2972 3180 3728 1517 Size: 1053 402 208 548 4741 Fragments arranged by size: 4741 1053 548 402 208 StyI C'CwwG_G Cuts at: 513 1065 2256 3189 3592 4391 513 Size: 552 1191 933 403 799 3074 Fragments arranged by size: 3074 1191 933 799 552 403 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2005 2762 4979 4996 5149 5334 5779 Size: 1255 425 757 2217 17 153 185 445 Cuts at: 5779 325 Size: 1498 Fragments arranged by size: 2217 1498 1255 757 445 425 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 3168 5198 Size: 274 80 33 51 1443 213 978 2030 Cuts at: 5198 6910 96 Size: 1712 138 Fragments arranged by size: 2030 1712 1443 978 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6912 6944 6912 Size: 32 6920 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6952 XbaI T'CTAG_A Cuts at: 4010 4288 4010 Size: 278 6674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6952 XmaI C'CCGG_G Cuts at: 1870 2350 2961 3066 4277 1870 Size: 480 611 105 1211 4545 Fragments arranged by size: 4545 1211 611 480 105 XmnI GAAnn'nnTTC Cuts at: 2135 5081 2135 Size: 2946 4006 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3676 4760 276 Size: 53 83 186 936 2142 1084 2468 Fragments arranged by size: 2468 2142 1084 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SfiI SrfI SwaI XcmI