(Circular) (Six-base) MAPSORT of: pFN21AA1361.seq Check: 1834 from: 1 to: 7708 pFN21AA1361 7708 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2545 4242 2545 Size: 1697 6011 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 5518 278 Size: 53 83 186 936 2287 1695 2468 Fragments arranged by size: 2468 2287 1695 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2201 5051 1937 Size: 264 2850 4594 Fragments arranged by size: 4594 2850 264 Acc65I G'GTAC_C Cuts at: 4864 5029 4864 Size: 165 7543 AclI AA'CG_TT Cuts at: 1563 5835 6208 1563 Size: 4272 373 3063 Fragments arranged by size: 4272 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2396 4830 5771 7155 7423 1632 Size: 422 342 2434 941 1384 268 1917 Fragments arranged by size: 2434 1917 1384 941 422 342 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7511 AflIII A'CryG_T Cuts at: 1203 2645 4995 6607 7584 1203 Size: 1442 2350 1612 977 1327 Fragments arranged by size: 2350 1612 1442 1327 977 AgeI A'CCGG_T Cuts at: 6539 6539 Size: 7708 AhdI GACnn_n'nnGTC Cuts at: 6437 6437 Size: 7708 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7708 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7708 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3978 4010 3978 Size: 32 7676 AlwNI CAG_nnn'CTG Cuts at: 4429 4909 4925 5451 7023 4429 Size: 480 16 526 1572 5114 Fragments arranged by size: 5114 1572 526 480 16 ApaLI G'TGCA_C Cuts at: 5765 6921 5765 Size: 1156 6552 ApoI r'AATT_y Cuts at: 1493 3608 5017 5243 5395 7504 1493 Size: 2115 1409 226 152 2109 1697 Fragments arranged by size: 2115 2109 1697 1409 226 152 AseI AT'TA_AT Cuts at: 160 1032 4341 6262 160 Size: 872 3309 1921 1606 Fragments arranged by size: 3309 1921 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7708 AvaI C'yCGr_G Cuts at: 1870 1943 3659 4717 4869 5033 1870 Size: 73 1716 1058 152 164 4545 Fragments arranged by size: 4545 1716 1058 164 152 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3712 3745 3712 Size: 33 7675 BamHI G'GATC_C Cuts at: 5038 5405 5038 Size: 367 7341 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4864 5029 6484 618 Size: 325 217 627 3077 165 1455 1842 Fragments arranged by size: 3077 1842 1455 627 325 217 165 BanII G_rGCy'C Cuts at: 1391 2058 4062 5027 1391 Size: 667 2004 965 4072 Fragments arranged by size: 4072 2004 965 667 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3107 928 Size: 971 1208 5529 Fragments arranged by size: 5529 1208 971 BbvCI CC'TCA_GC Cuts at: 4473 4473 Size: 7708 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3337 3371 5899 5933 1903 Size: 34 50 34 1316 34 2528 34 3678 Fragments arranged by size: 3678 2528 1316 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4180 5600 6810 4180 Size: 1420 1210 5078 Fragments arranged by size: 5078 1420 1210 BclI T'GATC_A Cuts at: 1548 4035 4330 1548 Size: 2487 295 4926 Fragments arranged by size: 4926 2487 295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6319 136 Size: 107 122 71 631 605 4647 1525 Fragments arranged by size: 4647 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2092 2878 3499 7703 2092 Size: 786 621 4204 2097 Fragments arranged by size: 4204 2097 786 621 BlpI GC'TnA_GC Cuts at: 4320 5125 4320 Size: 805 6903 Bme1580I G_kGCm'C Cuts at: 1790 4534 5769 6925 1790 Size: 2744 1235 1156 2573 Fragments arranged by size: 2744 2573 1235 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7708 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4758 4777 5439 Size: 416 53 83 399 24 3341 19 662 Cuts at: 5439 6397 442 Size: 958 1753 Fragments arranged by size: 3341 1753 958 662 416 399 83 53 24 19 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7708 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3391 3391 Size: 7708 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2774 4010 4259 4451 6368 1239 Size: 1535 1236 249 192 1917 2579 Fragments arranged by size: 2579 1917 1535 1236 249 192 Bpu10I CC'TnA_GC Cuts at: 4473 4473 Size: 7708 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2787 4268 5833 6698 6996 2787 Size: 1481 1565 865 298 3499 Fragments arranged by size: 3499 1565 1481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2637 3156 4522 6371 882 Size: 617 1138 519 1366 1849 2219 Fragments arranged by size: 2219 1849 1366 1138 617 519 BsaAI yAC'GTr Cuts at: 493 1204 2294 5499 493 Size: 711 1090 3205 2702 Fragments arranged by size: 3205 2702 1090 711 BsaBI GATnn'nnATC Cuts at: 3477 7702 3477 Size: 4225 3483 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3820 3880 5515 5897 Size: 53 83 186 936 2287 60 1635 382 Cuts at: 5897 6532 275 Size: 635 1451 Fragments arranged by size: 2287 1635 1451 936 635 382 186 83 60 53 BsaWI w'CCGG_w Cuts at: 4524 6141 6539 6813 6960 7371 4524 Size: 1617 398 274 147 411 4861 Fragments arranged by size: 4861 1617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7483 Fragments arranged by size: 7483 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3722 3926 1186 Size: 2536 204 4968 Fragments arranged by size: 4968 2536 204 BseYI C'CCAG_C Cuts at: 3068 4827 6911 7407 3068 Size: 1759 2084 496 3369 Fragments arranged by size: 3369 2084 1759 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5158 5919 6947 1540 Size: 275 186 3157 761 1028 2301 Fragments arranged by size: 3157 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2058 2410 4062 4263 5769 5854 6925 2058 Size: 352 1652 201 1506 85 1071 2841 Fragments arranged by size: 2841 1652 1506 1071 352 201 85 BsmI GAATG_Cn' Cuts at: 2695 3851 4447 4700 5219 5312 2695 Size: 1156 596 253 519 93 5091 Fragments arranged by size: 5091 1156 596 519 253 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2410 4062 4263 4534 5027 5769 Size: 399 268 352 1652 201 271 493 742 Cuts at: 5769 5854 6925 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1652 1071 742 493 399 352 271 268 201 85 BspEI T'CCGG_A Cuts at: 4524 7371 4524 Size: 2847 4861 BspHI T'CATG_A Cuts at: 5597 7327 5597 Size: 1730 5978 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2545 4199 4242 5063 844 Size: 1701 1654 43 821 3489 Fragments arranged by size: 3489 1701 1654 821 43 BsrBI CCG'CTC Cuts at: 1394 1823 5595 1394 Size: 429 3772 3507 Fragments arranged by size: 3772 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2700 6203 6377 65 Size: 1183 1452 3503 174 1396 Fragments arranged by size: 3503 1452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6352 6539 1700 Size: 110 4542 187 2869 Fragments arranged by size: 4542 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7708 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7708 BssSI C'ACGA_G Cuts at: 2139 4960 5768 6780 2139 Size: 2821 808 1012 3067 Fragments arranged by size: 3067 2821 1012 808 BstAPI GCAn_nnn'nTGC Cuts at: 794 2537 2699 3955 794 Size: 1743 162 1256 4547 Fragments arranged by size: 4547 1743 1256 162 BstBI TT'CG_AA Cuts at: 3688 6512 3688 Size: 2824 4884 BstEII G'GTnAC_C Cuts at: 1184 4838 1184 Size: 3654 4054 BstXI CCAn_nnnn'nTGG Cuts at: 2161 4800 2161 Size: 2639 5069 BstYI r'GATC_y Cuts at: 1972 2071 2092 2333 2878 3235 3256 3499 5038 Size: 99 21 241 545 357 21 243 1539 Cuts at: 5038 5405 5790 5807 7259 7345 7357 7703 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1539 1452 545 385 367 357 346 243 241 99 86 21 21 17 12 Bsu36I CC'TnA_GG Cuts at: 4695 4695 Size: 7708 BtgI C'CryG_G Cuts at: 513 1065 2110 2526 2592 4779 4832 513 Size: 552 1045 416 66 2187 53 3389 Fragments arranged by size: 3389 2187 1045 552 416 66 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3464 487 Size: 683 56 2238 4731 Fragments arranged by size: 4731 2238 683 56 BtsI GCAGTG_nn' Cuts at: 2133 5224 6018 6038 2133 Size: 3091 794 20 3803 Fragments arranged by size: 3803 3091 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7708 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7673 DraI TTT'AAA Cuts at: 2832 3201 5012 2832 Size: 369 1811 5528 Fragments arranged by size: 5528 1811 369 DraIII CAC_nnn'GTG Cuts at: 2405 3172 2405 Size: 767 6941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6715 809 Size: 507 5399 1802 Fragments arranged by size: 5399 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2166 3022 Size: 54 1425 50 275 18 277 59 856 Cuts at: 3022 5155 6044 7673 8 Size: 2133 889 1629 43 Fragments arranged by size: 2133 1629 1425 889 856 277 275 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5155 1537 Size: 275 3343 4090 Fragments arranged by size: 4090 3343 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2090 2415 2994 3222 3594 4084 4551 Size: 710 27 325 579 228 372 490 467 Cuts at: 4551 5638 1353 Size: 1087 3423 Fragments arranged by size: 3423 1087 710 579 490 467 372 325 228 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3986 4930 6295 6669 6815 387 Size: 1064 138 2397 944 1365 374 146 1280 Fragments arranged by size: 2397 1365 1280 1064 944 374 146 138 EcoICRI GAG'CTC Cuts at: 2056 4060 2056 Size: 2004 5704 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2396 2774 4010 4259 4451 4830 Size: 393 422 342 378 1236 249 192 379 Cuts at: 4830 5771 6368 7155 7423 1239 Size: 941 597 787 268 1524 Fragments arranged by size: 1524 1236 941 787 597 422 393 379 378 342 268 249 192 EcoNI CCTnn'n_nnAGG Cuts at: 2954 2954 Size: 7708 EcoO109I rG'GnC_Cy Cuts at: 2038 3898 4760 2038 Size: 1860 862 4986 Fragments arranged by size: 4986 1860 862 EcoRI G'AATT_C Cuts at: 5017 5395 5017 Size: 378 7330 EcoRV GAT'ATC Cuts at: 2458 2458 Size: 7708 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3716 3827 3716 Size: 111 7597 FspI TGC'GCA Cuts at: 6214 6214 Size: 7708 HaeII r_GCGC'y Cuts at: 1678 2860 6855 1678 Size: 1182 3995 2531 Fragments arranged by size: 3995 2531 1182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 2615 2647 3248 Size: 32 135 32 929 32 82 32 601 Cuts at: 3248 3280 6349 6381 6423 6455 1373 Size: 32 3069 32 42 32 2626 Fragments arranged by size: 3069 2626 929 601 135 82 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3109 5052 5298 669 Size: 988 281 1171 1943 246 3079 Fragments arranged by size: 3079 1943 1171 988 281 246 HindIII A'AGCT_T Cuts at: 748 2116 4632 4797 748 Size: 1368 2516 165 3659 Fragments arranged by size: 3659 2516 1368 165 HpaI GTT'AAC Cuts at: 3109 5298 3109 Size: 2189 5519 KpnI G_GTAC'C Cuts at: 4868 5033 4868 Size: 165 7543 MfeI C'AATT_G Cuts at: 5307 5307 Size: 7708 MluI A'CGCG_T Cuts at: 7584 7584 Size: 7708 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3028 3988 4587 6822 7006 1305 Size: 1723 960 599 2235 184 2007 Fragments arranged by size: 2235 2007 1723 960 599 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2109 2168 10 Size: 54 1425 343 277 59 5550 Fragments arranged by size: 5550 1425 343 277 59 54 MslI CAynn'nnrTG Cuts at: 518 1166 3173 5666 6025 6184 518 Size: 648 2007 2493 359 159 2042 Fragments arranged by size: 2493 2042 2007 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4072 4417 5124 5801 6949 7194 1606 Size: 2466 345 707 677 1148 245 2120 Fragments arranged by size: 2466 2120 1148 707 677 345 245 NcoI C'CATG_G Cuts at: 513 1065 2110 2526 2592 4779 4832 513 Size: 552 1045 416 66 2187 53 3389 Fragments arranged by size: 3389 2187 1045 552 416 66 53 NdeI CA'TA_TG Cuts at: 387 387 Size: 7708 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7708 NotI GC'GGCC_GC Cuts at: 5155 5155 Size: 7708 NruI TCG'CGA Cuts at: 4500 4500 Size: 7708 NspI r_CATG'y Cuts at: 1132 1229 2649 3076 3953 4118 4999 5066 6611 Size: 97 1420 427 877 165 881 67 1545 Cuts at: 6611 1132 Size: 2229 Fragments arranged by size: 2229 1545 1420 881 877 427 165 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7708 PciI A'CATG_T Cuts at: 2645 4995 6607 2645 Size: 2350 1612 3746 Fragments arranged by size: 3746 2350 1612 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7708 PfoI T'CCnGG_A Cuts at: 2955 4756 2955 Size: 1801 5907 PmeI GTTT'AAAC Cuts at: 5012 5012 Size: 7708 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3468 3500 5777 5809 7290 7322 3468 Size: 32 2277 32 1481 32 3854 Fragments arranged by size: 3854 2277 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2038 3898 4760 2038 Size: 1860 862 4986 Fragments arranged by size: 4986 1860 862 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7708 PsiI TTA'TAA Cuts at: 5278 5278 Size: 7708 PspXI vC'TCGA_Gb Cuts at: 4869 4869 Size: 7708 PstI C_TGCA'G Cuts at: 830 2928 3532 4422 5060 830 Size: 2098 604 890 638 3478 Fragments arranged by size: 3478 2098 890 638 604 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7708 PvuII CAG'CTG Cuts at: 4072 4417 4072 Size: 345 7363 SacI G_AGCT'C Cuts at: 2058 4062 2058 Size: 2004 5704 SalI G'TCGA_C Cuts at: 1936 5050 1936 Size: 3114 4594 SapI GCTCTTCn'nnn_ Cuts at: 2063 2090 2415 2063 Size: 27 325 7356 Fragments arranged by size: 7356 325 27 SbfI CC_TGCA'GG Cuts at: 5060 5060 Size: 7708 ScaI AGT'ACT Cuts at: 5956 7668 5956 Size: 1712 5996 SfcI C'TryA_G Cuts at: 826 1045 2738 2924 3528 4418 5056 6191 6872 Size: 219 1693 186 604 890 638 1135 681 Cuts at: 6872 7063 826 Size: 191 1471 Fragments arranged by size: 1693 1471 1135 890 681 638 604 219 191 186 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7708 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7708 SmaI CCC'GGG Cuts at: 1872 4719 5035 1872 Size: 2847 316 4545 Fragments arranged by size: 4545 2847 316 SmlI C'TyrA_G Cuts at: 820 1017 1943 2802 3659 4283 4869 5812 6713 Size: 197 926 859 857 624 586 943 901 Cuts at: 6713 6975 820 Size: 262 1553 Fragments arranged by size: 1553 943 926 901 859 857 624 586 262 197 SnaBI TAC'GTA Cuts at: 493 5499 493 Size: 5006 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7708 SphI G_CATG'C Cuts at: 1132 3953 5066 1132 Size: 2821 1113 3774 Fragments arranged by size: 3774 2821 1113 SspI AAT'ATT Cuts at: 5 52 2324 5632 5 Size: 47 2272 3308 2081 Fragments arranged by size: 3308 2272 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7708 StyI C'CwwG_G Cuts at: 513 1065 2110 2526 2592 4779 4832 5147 513 Size: 552 1045 416 66 2187 53 315 3074 Fragments arranged by size: 3074 2187 1045 552 416 315 66 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4772 5735 5752 5905 6090 6535 325 Size: 1255 3192 963 17 153 185 445 1498 Fragments arranged by size: 3192 1498 1255 963 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4212 5954 7666 Size: 274 80 33 51 1443 2235 1742 1712 Cuts at: 7666 96 Size: 138 Fragments arranged by size: 2235 1742 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4379 4411 7668 7700 4379 Size: 32 3257 32 4387 Fragments arranged by size: 4387 3257 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3902 1316 Size: 2586 5122 XbaI T'CTAG_A Cuts at: 5044 5044 Size: 7708 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2117 2161 2548 4764 4797 2117 Size: 44 387 2216 33 5028 Fragments arranged by size: 5028 2216 387 44 33 XhoI C'TCGA_G Cuts at: 1943 3659 4869 1943 Size: 1716 1210 4782 Fragments arranged by size: 4782 1716 1210 XmaI C'CCGG_G Cuts at: 1870 4717 5033 1870 Size: 2847 316 4545 Fragments arranged by size: 4545 2847 316 XmnI GAAnn'nnTTC Cuts at: 2084 5837 2084 Size: 3753 3955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 5516 276 Size: 53 83 186 936 2287 1695 2468 Fragments arranged by size: 2468 2287 1695 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI