Restriction map for
pFN21AA1333
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AocI ApaI ApaLI AscI Asp718I
AspHI AspI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I FbaI FseI FspI
HaeIII HapII Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 544 GCGG CCGC(-3/-1)
AflIII 900 ACATGT A^C[GA][CT]GT
AhdI 1525 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1681 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 767 AGCGCT AGC^GCT
AseI 1047 ATTAAT AT^TAAT
AsnI 1047 ATTAAT AT^TAAT
Asp700I 1888 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1525 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 985 GGATCC G^GATCC
BbsI 1888 GAAGAC GAAGAC(2/6)
BfaI 2083 CTAG C^TAG
BglII 999 AGATCT A^GATCT
BpmI 1928 CTCCAG CTGGAG(16/14)
BpuAI 1888 GAAGAC GAAGAC(2/6)
BsaBI 1488 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 810 CTCCTC GAGGAG(10/8)
BsmFI 374 GGGAC GGGAC(10/14)
BspLU11I 900 ACATGT A^CATGT
BstBI 704 TTCGAA TT^CGAA
CfoI 768 GCGC GCG^C
DraI 1939 TTTAAA TTT^AAA
DraII 1282 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1525 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 949 GAAGAG CTCTTC(1/4)
Eco47III 767 AGCGCT AGC^GCT
EcoO109I 1282 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 617 GAATTC G^AATTC
EcoRV 202 GATATC GAT^ATC
EcoT14I 1695 CCTTGG C^C[AT][AT]GG
EcoT22I 1130 ATGCAT ATGCA^T
FauI 544 GCGGG CCCGC(4/6)
HaeII 767 AGCGCT [GA]GCGC^[CT]
HgaI 361 GCGTC GACGC(5/10)
HhaI 768 GCGC GCG^C
HinP1I 768 GCGC G^CGC
HindIII 1823 AAGCTT A^AGCTT
Ksp632I 949 GAAGAG CTCTTC(1/4)
MaeI 2083 CTAG C^TAG
MamI 1488 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 284 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1931 CAGCTG C[AC]G^C[GT]G
NsiI 1130 ATGCAT ATGCA^T
NspV 704 TTCGAA TT^CGAA
PflMI 1106 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1282 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1047 ATTAAT AT^TAAT
PstI 679 CTGCAG CTGCA^G
PvuII 1931 CAGCTG CAG^CTG
SfuI 704 TTCGAA TT^CGAA
StyI 1695 CCTTGG C^C[AT][AT]GG
Van91I 1106 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1888 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbvI 681 GCAGC GCAGC(8/12)
1016 GCAGC
BsaJI 644 CCTGGG C^C[ACGT][ACGT]GG
1695 CCTTGG
BsmI 1546 GAATGC GAATGC(1/-1)
1132 GCATTC
Bsp1407I 117 TGTACA T^GTACA
565 TGTACA
BsrGI 117 TGTACA T^GTACA
565 TGTACA
BstAPI 431 GCACCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
535 GCAATAAATGC
BstXI 342 CCAGTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
434 CCATTTGCTTGG
BstYI 985 GGATCC [GA]^GATC[CT]
999 AGATCT
Fnu4HI 681 GCAGC GC^[ACGT]GC
1016 GCAGC
ItaI 681 GCAGC GC^[ACGT]GC
1016 GCAGC
MflI 985 GGATCC [GA]^GATC[CT]
999 AGATCT
NdeI 1160 CATATG CA^TATG
1178 CATATG
NspI 900 ACATGT [GA]CATG^[CT]
1968 GCATGT
SexAI 15 ACCTGGT A^CC[AT]GGT
1389 ACCTGGT
SfcI 679 CTGCAG C^T[GA][CT]AG
1517 CTGTAG
SspBI 117 TGTACA T^GTACA
565 TGTACA
TseI 681 GCAGC G^C[AT]GC
1016 GCAGC
XhoII 985 GGATCC [GA]^GATC[CT]
999 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII FokI HinfI
HphI MaeIII MboI MboII MfeI
MnlI MseI MunI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I