(Circular) (Six-base) MAPSORT of: pFN21AA1333.seq Check: 8586 from: 1 to: 6823 pFN21AA1333 6823 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4166 1937 Size: 2229 4594 Acc65I G'GTAC_C Cuts at: 4144 4144 Size: 6823 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2312 4886 6270 6538 1632 Size: 680 2574 1384 268 1917 Fragments arranged by size: 2574 1917 1384 680 268 AfeI AGC'GCT Cuts at: 2774 2774 Size: 6823 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6626 AflIII A'CryG_T Cuts at: 1203 2905 4120 5722 6699 1203 Size: 1702 1215 1602 977 1327 Fragments arranged by size: 1702 1602 1327 1215 977 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 3535 5552 3535 Size: 2017 4806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3934 1184 Size: 2750 4073 AlwNI CAG_nnn'CTG Cuts at: 3691 4566 6138 3691 Size: 875 1572 4376 Fragments arranged by size: 4376 1572 875 ApaLI G'TGCA_C Cuts at: 4880 6036 4880 Size: 1156 5667 ApoI r'AATT_y Cuts at: 1493 2154 2450 2622 3026 3236 3313 3717 4132 Size: 661 296 172 404 210 77 404 415 Cuts at: 4132 4358 4510 6619 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 661 415 404 404 296 226 210 172 152 77 AseI AT'TA_AT Cuts at: 160 1032 3053 5377 160 Size: 872 2021 2324 1606 Fragments arranged by size: 2324 2021 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 4148 1870 Size: 73 2205 4545 Fragments arranged by size: 4545 2205 73 BamHI G'GATC_C Cuts at: 2990 4153 4520 2990 Size: 1163 367 5293 Fragments arranged by size: 5293 1163 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4144 5599 618 Size: 325 217 627 2357 1455 1842 Fragments arranged by size: 2357 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4142 1391 Size: 2751 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3900 928 Size: 971 2001 3851 Fragments arranged by size: 3851 2001 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5014 5048 1903 Size: 34 3077 34 3678 Fragments arranged by size: 3678 3077 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4715 5925 4715 Size: 1210 5613 BclI T'GATC_A Cuts at: 1548 1548 Size: 6823 BfrBI ATG'CAT Cuts at: 3137 3137 Size: 6823 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5434 136 Size: 107 122 71 631 605 3762 1525 Fragments arranged by size: 3762 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3004 6818 3004 Size: 3814 3009 BlpI GC'TnA_GC Cuts at: 4240 4240 Size: 6823 Bme1580I G_kGCm'C Cuts at: 1790 4884 6040 1790 Size: 3094 1156 2573 Fragments arranged by size: 3094 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4554 5512 442 Size: 416 53 83 399 24 3137 958 1753 Fragments arranged by size: 3137 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3918 5483 1239 Size: 2679 1565 2579 Fragments arranged by size: 2679 2579 1565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3284 3303 3587 4948 5813 6111 3284 Size: 19 284 1361 865 298 3996 Fragments arranged by size: 3996 1361 865 298 284 19 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5486 882 Size: 617 3987 2219 Fragments arranged by size: 3987 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4614 493 Size: 711 3410 2702 Fragments arranged by size: 3410 2702 711 BsaBI GATnn'nnATC Cuts at: 3497 6817 3497 Size: 3320 3503 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4630 5012 5647 275 Size: 53 83 186 936 3097 382 635 1451 Fragments arranged by size: 3097 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5256 5654 5928 6075 6486 5256 Size: 398 274 147 411 5593 Fragments arranged by size: 5593 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6598 Fragments arranged by size: 6598 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2806 1186 Size: 1620 5203 BseYI C'CCAG_C Cuts at: 3450 6026 6522 3450 Size: 2576 496 3751 Fragments arranged by size: 3751 2576 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4273 5034 6062 1540 Size: 275 186 2272 761 1028 2301 Fragments arranged by size: 2301 2272 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4884 4969 6040 4884 Size: 85 1071 5667 Fragments arranged by size: 5667 1071 85 BsmI GAATG_Cn' Cuts at: 3137 3557 4334 4427 3137 Size: 420 777 93 5533 Fragments arranged by size: 5533 777 420 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4142 4884 4969 6040 1391 Size: 399 2352 742 85 1071 2174 Fragments arranged by size: 2352 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6486 6486 Size: 6823 BspHI T'CATG_A Cuts at: 4712 6442 4712 Size: 1730 5093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4178 844 Size: 3334 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4710 1394 Size: 429 2887 3507 Fragments arranged by size: 3507 2887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5318 5492 65 Size: 1183 4070 174 1396 Fragments arranged by size: 4070 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5467 5654 1700 Size: 110 3657 187 2869 Fragments arranged by size: 3657 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2122 2570 96 Size: 2026 448 4349 Fragments arranged by size: 4349 2026 448 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 4883 5895 4883 Size: 1012 5811 BstAPI GCAn_nnn'nTGC Cuts at: 794 2442 2546 794 Size: 1648 104 5071 Fragments arranged by size: 5071 1648 104 BstBI TT'CG_AA Cuts at: 2710 5627 2710 Size: 2917 3906 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6823 BstXI CCAn_nnnn'nTGG Cuts at: 2354 2446 2354 Size: 92 6731 BstYI r'GATC_y Cuts at: 1972 2990 3004 4153 4520 4905 4922 6374 6460 Size: 1018 14 1149 367 385 17 1452 86 Cuts at: 6460 6472 6818 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1149 1018 385 367 346 86 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6271 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6084 Fragments arranged by size: 6084 683 56 BtsI GCAGTG_nn' Cuts at: 4339 5133 5153 4339 Size: 794 20 6009 Fragments arranged by size: 6009 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6788 DraI TTT'AAA Cuts at: 3946 4127 3946 Size: 181 6642 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5830 809 Size: 507 4514 1802 Fragments arranged by size: 4514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4270 5159 6788 Size: 54 1425 50 275 18 2440 889 1629 Cuts at: 6788 8 Size: 43 Fragments arranged by size: 2440 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4270 1537 Size: 275 2458 4090 Fragments arranged by size: 4090 2458 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2949 4753 1353 Size: 1596 1804 3423 Fragments arranged by size: 3423 1804 1596 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5410 5784 5930 387 Size: 1064 138 3821 374 146 1280 Fragments arranged by size: 3821 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 3918 4886 5483 6270 6538 1239 Size: 393 680 1606 968 597 787 268 1524 Fragments arranged by size: 1606 1524 968 787 680 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3288 3288 Size: 6823 EcoRI G'AATT_C Cuts at: 2622 4132 4510 2622 Size: 1510 378 4935 Fragments arranged by size: 4935 1510 378 EcoRV GAT'ATC Cuts at: 2209 2209 Size: 6823 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3353 3650 3917 3353 Size: 297 267 6259 Fragments arranged by size: 6259 297 267 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 2776 5970 1678 Size: 1098 3194 2531 Fragments arranged by size: 3194 2531 1098 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2989 3021 3084 3116 3257 Size: 32 135 32 1417 32 63 32 141 Cuts at: 3257 3289 3521 3553 3860 3892 5464 5496 5538 Size: 32 232 32 307 32 1572 32 42 Cuts at: 5538 5570 1373 Size: 32 2626 Fragments arranged by size: 2626 1572 1417 307 232 141 135 63 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4167 4413 669 Size: 988 281 2229 246 3079 Fragments arranged by size: 3079 2229 988 281 246 HindIII A'AGCT_T Cuts at: 748 3828 748 Size: 3080 3743 HpaI GTT'AAC Cuts at: 4413 4413 Size: 6823 KpnI G_GTAC'C Cuts at: 4148 4148 Size: 6823 MfeI C'AATT_G Cuts at: 2274 3171 3960 4422 2274 Size: 897 789 462 4675 Fragments arranged by size: 4675 897 789 462 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2931 3970 5937 6121 1305 Size: 1626 1039 1967 184 2007 Fragments arranged by size: 2007 1967 1626 1039 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5001 Fragments arranged by size: 5001 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2293 4781 5140 5299 518 Size: 648 1127 2488 359 159 2042 Fragments arranged by size: 2488 2042 1127 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3938 4239 4916 6064 6309 1606 Size: 2332 301 677 1148 245 2120 Fragments arranged by size: 2332 2120 1148 677 301 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6271 NdeI CA'TA_TG Cuts at: 387 3166 3184 387 Size: 2779 18 4026 Fragments arranged by size: 4026 2779 18 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6823 NotI GC'GGCC_GC Cuts at: 4270 4270 Size: 6823 NsiI A_TGCA'T Cuts at: 3139 3139 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 2909 3977 4124 4181 5726 1132 Size: 97 1680 1068 147 57 1545 2229 Fragments arranged by size: 2229 1680 1545 1068 147 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6823 PciI A'CATG_T Cuts at: 2905 4120 5722 2905 Size: 1215 1602 4006 Fragments arranged by size: 4006 1602 1215 PflMI CCAn_nnn'nTGG Cuts at: 1108 3117 1108 Size: 2009 4814 PfoI T'CCnGG_A Cuts at: 3220 3220 Size: 6823 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4924 6405 6437 4892 Size: 32 1481 32 5278 Fragments arranged by size: 5278 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3288 3288 Size: 6823 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6823 PsiI TTA'TAA Cuts at: 2475 3497 3593 4393 2475 Size: 1022 96 800 4905 Fragments arranged by size: 4905 1022 800 96 PstI C_TGCA'G Cuts at: 830 2688 4175 830 Size: 1858 1487 3478 Fragments arranged by size: 3478 1858 1487 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 PvuII CAG'CTG Cuts at: 3938 3938 Size: 6823 SalI G'TCGA_C Cuts at: 1936 4165 1936 Size: 2229 4594 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 5071 6783 5071 Size: 1712 5111 SexAI A'CCwGG_T Cuts at: 2020 3394 2020 Size: 1374 5449 SfcI C'TryA_G Cuts at: 826 1045 2684 3522 4171 5306 5987 6178 826 Size: 219 1639 838 649 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 838 681 649 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6823 SmaI CCC'GGG Cuts at: 1872 4150 1872 Size: 2278 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3263 3318 3566 4927 5828 6090 Size: 197 926 1320 55 248 1361 901 262 Cuts at: 6090 820 Size: 1553 Fragments arranged by size: 1553 1361 1320 926 901 262 248 197 55 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 4181 1132 Size: 3049 3774 SspI AAT'ATT Cuts at: 5 52 4747 5 Size: 47 4695 2081 Fragments arranged by size: 4695 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6823 StyI C'CwwG_G Cuts at: 513 1065 3700 4262 513 Size: 552 2635 562 3074 Fragments arranged by size: 3074 2635 562 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4850 4867 5020 5205 5650 325 Size: 1255 3270 17 153 185 445 1498 Fragments arranged by size: 3270 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2122 2570 3805 Size: 274 80 33 51 1443 145 448 1235 Cuts at: 3805 5069 6781 96 Size: 1264 1712 138 Fragments arranged by size: 1712 1443 1264 1235 448 274 145 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6783 6815 6783 Size: 32 6791 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6823 XbaI T'CTAG_A Cuts at: 4159 4159 Size: 6823 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 4148 1870 Size: 2278 4545 XmnI GAAnn'nnTTC Cuts at: 3897 4952 3897 Size: 1055 5768 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI