(Circular) (Six-base) MAPSORT of: pFN21AA1332.seq Check: 7162 from: 1 to: 6856 pFN21AA1332 6856 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4666 278 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4199 1937 Size: 2262 4594 Acc65I G'GTAC_C Cuts at: 4177 4177 Size: 6856 AclI AA'CG_TT Cuts at: 1563 4983 5356 1563 Size: 3420 373 3063 Fragments arranged by size: 3420 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2487 3049 3249 3438 4919 6303 6571 1632 Size: 855 562 200 189 1481 1384 268 1917 Fragments arranged by size: 1917 1481 1384 855 562 268 200 189 AfeI AGC'GCT Cuts at: 2360 2360 Size: 6856 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6659 AflIII A'CryG_T Cuts at: 1203 5755 6732 1203 Size: 4552 977 1327 Fragments arranged by size: 4552 1327 977 AgeI A'CCGG_T Cuts at: 5687 5687 Size: 6856 AhdI GACnn_n'nnGTC Cuts at: 5585 5585 Size: 6856 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6856 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3950 1184 Size: 2766 4090 AlwNI CAG_nnn'CTG Cuts at: 3821 4599 6171 3821 Size: 778 1572 4506 Fragments arranged by size: 4506 1572 778 ApaI G_GGCC'C Cuts at: 3013 3013 Size: 6856 ApaLI G'TGCA_C Cuts at: 4913 6069 4913 Size: 1156 5700 ApoI r'AATT_y Cuts at: 1493 4165 4391 4543 6652 1493 Size: 2672 226 152 2109 1697 Fragments arranged by size: 2672 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5410 160 Size: 872 4378 1606 Fragments arranged by size: 4378 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 AvaI C'yCGr_G Cuts at: 1870 1943 2850 3162 3273 4181 1870 Size: 73 907 312 111 908 4545 Fragments arranged by size: 4545 908 907 312 111 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2222 2255 2222 Size: 33 6823 BamHI G'GATC_C Cuts at: 2487 2759 3497 4186 4553 2487 Size: 272 738 689 367 4790 Fragments arranged by size: 4790 738 689 367 272 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3024 3665 4177 5632 618 Size: 325 217 627 1237 641 512 1455 1842 Fragments arranged by size: 1842 1455 1237 641 627 512 325 217 BanII G_rGCy'C Cuts at: 1391 2100 3013 3091 3250 4175 1391 Size: 709 913 78 159 925 4072 Fragments arranged by size: 4072 925 913 709 159 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3055 3744 928 Size: 971 1156 689 4040 Fragments arranged by size: 4040 1156 971 689 BbvCI CC'TCA_GC Cuts at: 2118 2118 Size: 6856 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5047 5081 1903 Size: 34 3110 34 3678 Fragments arranged by size: 3678 3110 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4748 5958 3416 Size: 1332 1210 4314 Fragments arranged by size: 4314 1332 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6856 BfrBI ATG'CAT Cuts at: 2956 2956 Size: 6856 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3867 5467 136 Size: 107 122 71 631 605 2195 1600 1525 Fragments arranged by size: 2195 1600 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3484 3505 6851 3484 Size: 21 3346 3489 Fragments arranged by size: 3489 3346 21 BlpI GC'TnA_GC Cuts at: 4273 4273 Size: 6856 Bme1580I G_kGCm'C Cuts at: 1790 3013 3643 3670 3804 4917 6073 1790 Size: 1223 630 27 134 1113 1156 2573 Fragments arranged by size: 2573 1223 1156 1113 630 134 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6856 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2099 2832 3096 Size: 416 53 83 399 24 682 733 264 Cuts at: 3096 4587 5545 442 Size: 1491 958 1753 Fragments arranged by size: 1753 1491 958 733 682 416 399 264 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6856 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3110 3190 3354 3609 3110 Size: 80 164 255 6357 Fragments arranged by size: 6357 255 164 80 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2479 2550 3126 3168 3324 3741 5516 1239 Size: 1240 71 576 42 156 417 1775 2579 Fragments arranged by size: 2579 1775 1240 576 417 156 71 42 Bpu10I CC'TnA_GC Cuts at: 2118 2118 Size: 6856 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2819 2969 4981 5846 6144 2819 Size: 150 2012 865 298 3531 Fragments arranged by size: 3531 2012 865 298 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3232 5519 882 Size: 617 1733 2287 2219 Fragments arranged by size: 2287 2219 1733 617 BsaAI yAC'GTr Cuts at: 493 1204 3631 3946 4647 493 Size: 711 2427 315 701 2702 Fragments arranged by size: 2702 2427 711 701 315 BsaBI GATnn'nnATC Cuts at: 2391 3496 6850 2391 Size: 1105 3354 2397 Fragments arranged by size: 3354 2397 1105 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4663 5045 5680 275 Size: 53 83 186 936 3130 382 635 1451 Fragments arranged by size: 3130 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5289 5687 5961 6108 6519 5289 Size: 398 274 147 411 5626 Fragments arranged by size: 5626 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3763 3783 3793 3813 1178 Size: 30 165 30 2360 20 10 20 4221 Fragments arranged by size: 4221 2360 165 30 30 20 20 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2005 2108 2135 2701 3129 3315 3732 1186 Size: 819 103 27 566 428 186 417 4310 Fragments arranged by size: 4310 819 566 428 417 186 103 27 BseYI C'CCAG_C Cuts at: 3225 3292 6059 6555 3225 Size: 67 2767 496 3526 Fragments arranged by size: 3526 2767 496 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6283 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 4306 5067 6095 1540 Size: 275 186 1222 1083 761 1028 2301 Fragments arranged by size: 2301 1222 1083 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4917 5002 6073 4917 Size: 85 1071 5700 Fragments arranged by size: 5700 1071 85 BsmI GAATG_Cn' Cuts at: 2956 4367 4460 2956 Size: 1411 93 5352 Fragments arranged by size: 5352 1411 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3297 3661 3297 Size: 364 6492 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 3013 3091 3250 3643 3670 3804 Size: 399 310 913 78 159 393 27 134 Cuts at: 3804 4175 4917 5002 6073 1391 Size: 371 742 85 1071 2174 Fragments arranged by size: 2174 1071 913 742 399 393 371 310 159 134 85 78 27 BspEI T'CCGG_A Cuts at: 6519 6519 Size: 6856 BspHI T'CATG_A Cuts at: 2283 4745 6475 2283 Size: 2462 1730 2664 Fragments arranged by size: 2664 2462 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4211 844 Size: 3367 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3174 4743 1394 Size: 429 1351 1569 3507 Fragments arranged by size: 3507 1569 1351 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2443 2782 3852 5351 5525 65 Size: 1183 1195 339 1070 1499 174 1396 Fragments arranged by size: 1499 1396 1195 1183 1070 339 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5500 5687 1700 Size: 110 3690 187 2869 Fragments arranged by size: 3690 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2547 3382 96 Size: 2451 835 3570 Fragments arranged by size: 3570 2451 835 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6856 BssSI C'ACGA_G Cuts at: 4916 5928 4916 Size: 1012 5844 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6856 BstBI TT'CG_AA Cuts at: 5660 5660 Size: 6856 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6856 BstXI CCAn_nnnn'nTGG Cuts at: 3021 3833 3021 Size: 812 6044 BstYI r'GATC_y Cuts at: 1972 2487 2759 3484 3497 3505 3517 4186 4553 Size: 515 272 725 13 8 12 669 367 Cuts at: 4553 4938 4955 6407 6493 6505 6851 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 725 669 515 385 367 346 272 86 17 13 12 12 8 BtgI C'CryG_G Cuts at: 513 1065 2004 2683 2697 3546 4044 513 Size: 552 939 679 14 849 498 3325 Fragments arranged by size: 3325 939 849 679 552 498 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3713 487 Size: 683 56 2487 3630 Fragments arranged by size: 3630 2487 683 56 BtsI GCAGTG_nn' Cuts at: 2810 3793 4372 5166 5186 2810 Size: 983 579 794 20 4480 Fragments arranged by size: 4480 983 794 579 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6856 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6821 DraI TTT'AAA Cuts at: 4160 4160 Size: 6856 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5863 809 Size: 507 4547 1802 Fragments arranged by size: 4547 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2585 2704 Size: 54 1425 50 275 18 177 578 119 Cuts at: 2704 2857 3220 3633 4303 5192 6821 8 Size: 153 363 413 670 889 1629 43 Fragments arranged by size: 1629 1425 889 670 578 413 363 275 177 153 119 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 4303 1537 Size: 275 1408 1083 4090 Fragments arranged by size: 4090 1408 1083 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 2994 3370 4786 1353 Size: 801 840 376 1416 3423 Fragments arranged by size: 3423 1416 840 801 376 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5443 5817 5963 387 Size: 1064 138 3854 374 146 1280 Fragments arranged by size: 3854 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2479 2487 2550 3049 3126 3168 3249 Size: 393 847 8 63 499 77 42 81 Cuts at: 3249 3324 3438 3741 4919 5516 6303 6571 1239 Size: 75 114 303 1178 597 787 268 1524 Fragments arranged by size: 1524 1178 847 787 597 499 393 303 268 114 81 77 75 63 42 8 EcoNI CCTnn'n_nnAGG Cuts at: 3813 3825 3813 Size: 12 6844 EcoO109I rG'GnC_Cy Cuts at: 2508 2788 3009 3010 3713 3818 2508 Size: 280 221 1 703 105 5546 Fragments arranged by size: 5546 703 280 221 105 1 EcoRI G'AATT_C Cuts at: 4165 4543 4165 Size: 378 6478 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3302 3302 Size: 6856 FspI TGC'GCA Cuts at: 5362 5362 Size: 6856 HaeII r_GCGC'y Cuts at: 1678 2362 6003 1678 Size: 684 3641 2531 Fragments arranged by size: 3641 2531 684 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2100 5497 5529 5571 Size: 32 135 32 496 32 3397 32 42 Cuts at: 5571 5603 1373 Size: 32 2626 Fragments arranged by size: 3397 2626 496 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3217 4200 4446 669 Size: 988 281 1279 983 246 3079 Fragments arranged by size: 3079 1279 988 983 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6856 HpaI GTT'AAC Cuts at: 3217 4446 3217 Size: 1229 5627 KpnI G_GTAC'C Cuts at: 4181 4181 Size: 6856 MfeI C'AATT_G Cuts at: 4455 4455 Size: 6856 MluI A'CGCG_T Cuts at: 6732 6732 Size: 6856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 2515 3656 3997 5970 6154 1305 Size: 822 388 1141 341 1973 184 2007 Fragments arranged by size: 2007 1973 1141 822 388 341 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2706 2859 3635 10 Size: 54 1425 343 177 697 153 776 3231 Fragments arranged by size: 3231 1425 776 697 343 177 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 3938 4814 5173 5332 518 Size: 648 879 1893 876 359 159 2042 Fragments arranged by size: 2042 1893 879 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2433 3225 3353 4272 4949 6097 6342 1606 Size: 827 792 128 919 677 1148 245 2120 Fragments arranged by size: 2120 1148 919 827 792 677 245 128 NcoI C'CATG_G Cuts at: 513 1065 2004 2683 2697 3546 513 Size: 552 939 679 14 849 3823 Fragments arranged by size: 3823 939 849 679 552 14 NdeI CA'TA_TG Cuts at: 387 387 Size: 6856 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6856 NotI GC'GGCC_GC Cuts at: 4303 4303 Size: 6856 NsiI A_TGCA'T Cuts at: 2958 2958 Size: 6856 NspI r_CATG'y Cuts at: 1132 1229 2379 2956 4214 5759 1132 Size: 97 1150 577 1258 1545 2229 Fragments arranged by size: 2229 1545 1258 1150 577 97 PasI CC'CwG_GG Cuts at: 1348 3243 3815 1348 Size: 1895 572 4389 Fragments arranged by size: 4389 1895 572 PciI A'CATG_T Cuts at: 5755 5755 Size: 6856 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6856 PfoI T'CCnGG_A Cuts at: 2295 2338 3340 2295 Size: 43 1002 5811 Fragments arranged by size: 5811 1002 43 PmeI GTTT'AAAC Cuts at: 4160 4160 Size: 6856 PmlI CAC'GTG Cuts at: 3631 3631 Size: 6856 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4925 4957 6438 6470 4925 Size: 32 1481 32 5311 Fragments arranged by size: 5311 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2508 2788 2508 Size: 280 6576 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6856 PsiI TTA'TAA Cuts at: 4426 4426 Size: 6856 PspOMI G'GGCC_C Cuts at: 3009 3009 Size: 6856 PspXI vC'TCGA_Gb Cuts at: 2850 2850 Size: 6856 PstI C_TGCA'G Cuts at: 830 3730 4208 830 Size: 2900 478 3478 Fragments arranged by size: 3478 2900 478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6856 PvuII CAG'CTG Cuts at: 2433 3353 2433 Size: 920 5936 SalI G'TCGA_C Cuts at: 1936 4198 1936 Size: 2262 4594 SapI GCTCTTCn'nnn_ Cuts at: 2994 3370 2994 Size: 376 6480 SbfI CC_TGCA'GG Cuts at: 4208 4208 Size: 6856 ScaI AGT'ACT Cuts at: 3403 4112 5104 6816 3403 Size: 709 992 1712 3443 Fragments arranged by size: 3443 1712 992 709 SfcI C'TryA_G Cuts at: 826 1045 3726 4204 5339 6020 6211 826 Size: 219 2681 478 1135 681 191 1471 Fragments arranged by size: 2681 1471 1135 681 478 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6856 SmaI CCC'GGG Cuts at: 1872 3275 4183 1872 Size: 1403 908 4545 Fragments arranged by size: 4545 1403 908 SmlI C'TyrA_G Cuts at: 820 1017 1943 2798 2850 2984 4960 5861 6123 Size: 197 926 855 52 134 1976 901 262 Cuts at: 6123 820 Size: 1553 Fragments arranged by size: 1976 1553 926 901 855 262 197 134 52 SnaBI TAC'GTA Cuts at: 493 4647 493 Size: 4154 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6856 SphI G_CATG'C Cuts at: 1132 2379 4214 1132 Size: 1247 1835 3774 Fragments arranged by size: 3774 1835 1247 SspI AAT'ATT Cuts at: 5 52 4780 5 Size: 47 4728 2081 Fragments arranged by size: 4728 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6856 StyI C'CwwG_G Cuts at: 513 1065 2004 2683 2697 2966 3546 3826 3966 Size: 552 939 679 14 269 580 280 140 Cuts at: 3966 4295 513 Size: 329 3074 Fragments arranged by size: 3074 939 679 580 552 329 280 269 140 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2118 3347 4883 4900 5053 5238 5683 Size: 1255 538 1229 1536 17 153 185 445 Cuts at: 5683 325 Size: 1498 Fragments arranged by size: 1536 1498 1255 1229 538 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 3382 3401 Size: 274 80 33 51 1443 570 835 19 Cuts at: 3401 4110 4134 5102 6814 96 Size: 709 24 968 1712 138 Fragments arranged by size: 1712 1443 968 835 709 570 274 138 80 51 33 24 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3039 3071 3783 3815 6816 6848 3039 Size: 32 712 32 3001 32 3047 Fragments arranged by size: 3047 3001 712 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6856 XbaI T'CTAG_A Cuts at: 4192 4192 Size: 6856 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3077 3553 3077 Size: 476 6380 XhoI C'TCGA_G Cuts at: 1943 2850 1943 Size: 907 5949 XmaI C'CCGG_G Cuts at: 1870 3273 4181 1870 Size: 1403 908 4545 Fragments arranged by size: 4545 1403 908 XmnI GAAnn'nnTTC Cuts at: 4985 4985 Size: 6856 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4664 276 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI