Restriction map for
pFN21AA1321
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 391 GTAGAC GT^[AC][GT]AC
Aor51HI 1212 AGCGCT AGC^GCT
Asp700I 1648 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1449 GAGCAC G[AT]GC[AT]^C
BanII 1540 GAGCCC G[GA]GC[CT]^C
BsaI 742 GAGACC GGTCTC(1/5)
BseRI 4 GAGGAG GAGGAG(10/8)
BsgI 718 GTGCAG GTGCAG(16/14)
BsiHKAI 1449 GAGCAC G[AT]GC[AT]^C
BsmI 1733 GCATTC GAATGC(1/-1)
BsrBI 645 GAGCGG CCGCTC(-3/-3)
BssSI 996 CTCGTG CACGAG(-5/-1)
BstAPI 1434 GCAAACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 825 TTCGAA TT^CGAA
BstEII 1795 GGTGACC G^GT[ACGT]ACC
BstPI 1795 GGTGACC G^GT[ACGT]ACC
DraIII 1637 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2066 CCATGG C^C[GA][CT]GG
EaeI 15 CGGCCA [CT]^GGCC[GA]
Eco47III 1212 AGCGCT AGC^GCT
EcoO65I 1795 GGTGACC G^GT[ACGT]ACC
EcoRV 1501 GATATC GAT^ATC
HaeII 1212 AGCGCT [GA]GCGC^[CT]
MslI 1789 CATATAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2066 CCATGG C^CATGG
NspI 966 GCATGC [GA]CATG^[CT]
NspV 825 TTCGAA TT^CGAA
SexAI 921 ACCTGGT A^CC[AT]GGT
SfaNI 1671 GCATC GCATC(5/9)
SfuI 825 TTCGAA TT^CGAA
SmlI 1718 CTCAAG C^T[CT][GA]AG
SphI 966 GCATGC GCATG^C
StuI 1935 AGGCCT AGG^CCT
XmnI 1648 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcsI 456 GAATTT [GA]^AATT[CT]
2019 GAATTT
AocI 1658 CCTCAGG CC^T[ACGT]AGG
1971 CCTGAGG
ApoI 456 GAATTT [GA]^AATT[CT]
2019 GAATTT
BbvCI 28 CCTCAGC CCTCAGC(-5/-2)
67 CCTCAGC
BcnI 13 CCCGG CC^[GC]GG
1160 CCGGG
BmyI 1449 GAGCAC G[AGT]GC[ACT]^C
1540 GAGCCC
BsmAI 742 GAGAC GTCTC(1/5)
1558 GAGAC
Bsp1286I 1449 GAGCAC G[AGT]GC[ACT]^C
1540 GAGCCC
BstF5I 52 CATCC GGATG(2/0)
1672 CATCC
Bsu36I 1658 CCTCAGG CC^T[ACGT]AGG
1971 CCTGAGG
CfoI 681 GCGC GCG^C
1213 GCGC
DraI 321 TTTAAA TTT^AAA
1009 TTTAAA
DraII 365 GGGTCCT [GA]G^G[ACGT]CC[CT]
1199 AGGTCCC
EarI 1116 CTCTTC CTCTTC(1/4)
1338 CTCTTC
Eco81I 1658 CCTCAGG CC^T[ACGT]AGG
1971 CCTGAGG
EcoO109I 365 GGGTCCT [GA]G^G[ACGT]CC[CT]
1199 AGGTCCC
FokI 52 CATCC GGATG(9/13)
1672 CATCC
HapII 14 CCGG C^CGG
1160 CCGG
HhaI 681 GCGC GCG^C
1213 GCGC
HinP1I 681 GCGC G^CGC
1213 GCGC
HpaII 14 CCGG C^CGG
1160 CCGG
Ksp632I 1116 CTCTTC CTCTTC(1/4)
1338 CTCTTC
MspI 14 CCGG C^CGG
1160 CCGG
NciI 13 CCCGG CC^[GC]GG
1160 CCGGG
PleI 994 GACTC GAGTC(4/5)
1371 GAGTC
PpuMI 365 GGGTCCT [GA]G^G[AT]CC[CT]
1199 AGGTCCC
PshAI 744 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1366 GACATGAGTC
PstI 172 CTGCAG CTGCA^G
1801 CTGCAG
PvuII 974 CAGCTG CAG^CTG
1385 CAGCTG
SpeI 1711 ACTAGT A^CTAGT
1753 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BfaI BpmI BsaJI BsiYI
BslI BsmFI BspMI BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII