(Circular) (Six-base) MAPSORT of: pFN21AA1321.seq Check: 4061 from: 1 to: 6790 pFN21AA1321 6790 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4600 278 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2397 4133 1937 Size: 460 1736 4594 Fragments arranged by size: 4594 1736 460 Acc65I G'GTAC_C Cuts at: 4111 4111 Size: 6790 AclI AA'CG_TT Cuts at: 1563 4917 5290 1563 Size: 3354 373 3063 Fragments arranged by size: 3354 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 2961 3369 4853 6237 6505 1632 Size: 797 532 408 1484 1384 268 1917 Fragments arranged by size: 1917 1484 1384 797 532 408 268 AfeI AGC'GCT Cuts at: 3219 3219 Size: 6790 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6593 AflIII A'CryG_T Cuts at: 1203 5689 6666 1203 Size: 4486 977 1327 Fragments arranged by size: 4486 1327 977 AgeI A'CCGG_T Cuts at: 5621 5621 Size: 6790 AhdI GACnn_n'nnGTC Cuts at: 5519 5519 Size: 6790 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6790 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6790 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3661 3693 3661 Size: 32 6758 AlwNI CAG_nnn'CTG Cuts at: 3272 3307 3392 3671 4533 6105 3272 Size: 35 85 279 862 1572 3957 Fragments arranged by size: 3957 1572 862 279 85 35 ApaLI G'TGCA_C Cuts at: 4847 6003 4847 Size: 1156 5634 ApoI r'AATT_y Cuts at: 1493 2461 4024 4099 4325 4477 6586 1493 Size: 968 1563 75 226 152 2109 1697 Fragments arranged by size: 2109 1697 1563 968 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 5344 160 Size: 872 4312 1606 Fragments arranged by size: 4312 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 AvaI C'yCGr_G Cuts at: 1870 1943 4115 1870 Size: 73 2172 4545 Fragments arranged by size: 4545 2172 73 BamHI G'GATC_C Cuts at: 4120 4487 4120 Size: 367 6423 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4111 5566 618 Size: 325 217 627 2324 1455 1842 Fragments arranged by size: 2324 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3549 4109 1391 Size: 2158 560 4072 Fragments arranged by size: 4072 2158 560 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5819 BbvCI CC'TCA_GC Cuts at: 2034 2073 2034 Size: 39 6751 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4981 5015 1903 Size: 34 3044 34 3678 Fragments arranged by size: 3678 3044 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4682 5892 4682 Size: 1210 5580 BclI T'GATC_A Cuts at: 1548 1548 Size: 6790 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5401 136 Size: 107 122 71 631 605 3729 1525 Fragments arranged by size: 3729 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6785 6785 Size: 6790 BlpI GC'TnA_GC Cuts at: 4207 4207 Size: 6790 Bme1580I G_kGCm'C Cuts at: 1790 4851 6007 1790 Size: 3061 1156 2573 Fragments arranged by size: 3061 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6790 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2865 3020 3543 Size: 416 53 83 399 24 1448 155 523 Cuts at: 3543 3582 3783 4521 5479 442 Size: 39 201 738 958 1753 Fragments arranged by size: 1753 1448 958 738 523 416 399 201 155 83 53 39 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6790 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2328 2541 2883 3276 3350 3725 5450 Size: 992 97 213 342 393 74 375 1725 Cuts at: 5450 1239 Size: 2579 Fragments arranged by size: 2579 1725 992 393 375 342 213 97 74 Bpu10I CC'TnA_GC Cuts at: 2034 2073 2034 Size: 39 6751 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3708 4915 5780 6078 3708 Size: 1207 865 298 4420 Fragments arranged by size: 4420 1207 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2741 5453 882 Size: 617 1242 2712 2219 Fragments arranged by size: 2712 2219 1242 617 BsaAI yAC'GTr Cuts at: 493 1204 4581 493 Size: 711 3377 2702 Fragments arranged by size: 3377 2702 711 BsaBI GATnn'nnATC Cuts at: 6784 6784 Size: 6790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4597 4979 5614 275 Size: 53 83 186 936 3064 382 635 1451 Fragments arranged by size: 3064 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5223 5621 5895 6042 6453 5223 Size: 398 274 147 411 5560 Fragments arranged by size: 5560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2229 2259 1178 Size: 30 165 30 826 30 5709 Fragments arranged by size: 5709 826 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 1186 Size: 838 5952 BseYI C'CCAG_C Cuts at: 2208 3960 5993 6489 2208 Size: 1752 2033 496 2509 Fragments arranged by size: 2509 2033 1752 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2744 1163 Size: 573 1008 5209 Fragments arranged by size: 5209 1008 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4240 5001 6029 1540 Size: 275 186 2239 761 1028 2301 Fragments arranged by size: 2301 2239 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3458 4851 4936 6007 3458 Size: 1393 85 1071 4241 Fragments arranged by size: 4241 1393 1071 85 BsmI GAATG_Cn' Cuts at: 3738 4301 4394 3738 Size: 563 93 6134 Fragments arranged by size: 6134 563 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3458 3549 4109 4851 4936 6007 1391 Size: 399 1668 91 560 742 85 1071 2174 Fragments arranged by size: 2174 1668 1071 742 560 399 91 85 BspEI T'CCGG_A Cuts at: 6453 6453 Size: 6790 BspHI T'CATG_A Cuts at: 4679 6409 4679 Size: 1730 5060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2184 2893 3813 4145 844 Size: 1340 709 920 332 3489 Fragments arranged by size: 3489 1340 920 709 332 BsrBI CCG'CTC Cuts at: 1394 1823 2652 4677 1394 Size: 429 829 2025 3507 Fragments arranged by size: 3507 2025 829 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5285 5459 65 Size: 1183 4037 174 1396 Fragments arranged by size: 4037 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5434 5621 1700 Size: 110 3624 187 2869 Fragments arranged by size: 3624 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6790 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6790 BssSI C'ACGA_G Cuts at: 3001 4850 5862 3001 Size: 1849 1012 3929 Fragments arranged by size: 3929 1849 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3445 794 Size: 2651 4139 BstBI TT'CG_AA Cuts at: 2831 5594 2831 Size: 2763 4027 BstEII G'GTnAC_C Cuts at: 1184 3800 1184 Size: 2616 4174 BstYI r'GATC_y Cuts at: 1972 2642 3469 4039 4120 4487 4872 4889 6341 Size: 670 827 570 81 367 385 17 1452 Cuts at: 6341 6427 6439 6785 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 827 670 570 385 367 346 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 3664 3977 3664 Size: 313 6477 BtgI C'CryG_G Cuts at: 513 1065 2004 4071 513 Size: 552 939 2067 3232 Fragments arranged by size: 3232 2067 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6051 Fragments arranged by size: 6051 683 56 BtsI GCAGTG_nn' Cuts at: 3055 3305 4306 5100 5120 3055 Size: 250 1001 794 20 4725 Fragments arranged by size: 4725 1001 794 250 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6790 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2553 2588 529 Size: 35 1989 35 4731 Fragments arranged by size: 4731 1989 35 35 DraI TTT'AAA Cuts at: 2328 3016 4094 2328 Size: 688 1078 5024 Fragments arranged by size: 5024 1078 688 DraIII CAC_nnn'GTG Cuts at: 3647 3647 Size: 6790 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5797 809 Size: 507 4481 1802 Fragments arranged by size: 4481 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2020 4237 5126 Size: 54 1425 50 275 18 190 2217 889 Cuts at: 5126 6755 8 Size: 1629 43 Fragments arranged by size: 2217 1629 1425 889 275 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4237 1537 Size: 275 2425 4090 Fragments arranged by size: 4090 2425 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3127 3349 4720 1353 Size: 1774 222 1371 3423 Fragments arranged by size: 3423 1774 1371 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5377 5751 5897 387 Size: 1064 138 3788 374 146 1280 Fragments arranged by size: 3788 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2328 2429 2541 2883 2961 3276 Size: 393 599 97 101 112 342 78 315 Cuts at: 3276 3350 3369 3725 4853 5450 6237 6505 1239 Size: 74 19 356 1128 597 787 268 1524 Fragments arranged by size: 1524 1128 787 599 597 393 356 342 315 268 112 101 97 78 74 19 EcoO109I rG'GnC_Cy Cuts at: 2371 3205 2371 Size: 834 5956 EcoRI G'AATT_C Cuts at: 4099 4477 4099 Size: 378 6412 EcoRV GAT'ATC Cuts at: 3508 3508 Size: 6790 FspI TGC'GCA Cuts at: 5296 5296 Size: 6790 HaeII r_GCGC'y Cuts at: 1678 3221 5937 1678 Size: 1543 2716 2531 Fragments arranged by size: 2716 2531 1543 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2516 2548 3461 3493 3706 Size: 32 135 32 944 32 913 32 213 Cuts at: 3706 3738 3856 3888 5431 5463 5505 5537 1373 Size: 32 118 32 1543 32 42 32 2626 Fragments arranged by size: 2626 1543 944 913 213 135 118 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4134 4380 669 Size: 988 281 2196 246 3079 Fragments arranged by size: 3079 2196 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6790 HpaI GTT'AAC Cuts at: 4380 4380 Size: 6790 KpnI G_GTAC'C Cuts at: 4115 4115 Size: 6790 MfeI C'AATT_G Cuts at: 4389 4389 Size: 6790 MluI A'CGCG_T Cuts at: 6666 6666 Size: 6790 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5904 6088 1305 Size: 4599 184 2007 Fragments arranged by size: 4599 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4968 Fragments arranged by size: 4968 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3798 4748 5107 5266 518 Size: 648 2632 950 359 159 2042 Fragments arranged by size: 2632 2042 950 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2221 2981 3392 4206 4883 6031 6276 1606 Size: 615 760 411 814 677 1148 245 2120 Fragments arranged by size: 2120 1148 814 760 677 615 411 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4071 513 Size: 552 939 2067 3232 Fragments arranged by size: 3232 2067 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6790 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6790 NotI GC'GGCC_GC Cuts at: 4237 4237 Size: 6790 NspI r_CATG'y Cuts at: 1132 1229 2975 4148 5693 1132 Size: 97 1746 1173 1545 2229 Fragments arranged by size: 2229 1746 1545 1173 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6790 PciI A'CATG_T Cuts at: 5689 5689 Size: 6790 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6790 PfoI T'CCnGG_A Cuts at: 3366 3366 Size: 6790 PmeI GTTT'AAAC Cuts at: 4094 4094 Size: 6790 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4859 4891 6372 6404 4859 Size: 32 1481 32 5245 Fragments arranged by size: 5245 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2371 3205 2371 Size: 834 5956 PshAI GACnn'nnGTC Cuts at: 1613 2753 3375 1613 Size: 1140 622 5028 Fragments arranged by size: 5028 1140 622 PsiI TTA'TAA Cuts at: 3539 4360 3539 Size: 821 5969 PstI C_TGCA'G Cuts at: 830 2181 3810 4142 830 Size: 1351 1629 332 3478 Fragments arranged by size: 3478 1629 1351 332 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6790 PvuII CAG'CTG Cuts at: 2981 3392 2981 Size: 411 6379 SalI G'TCGA_C Cuts at: 1936 4132 1936 Size: 2196 4594 SbfI CC_TGCA'GG Cuts at: 4142 4142 Size: 6790 ScaI AGT'ACT Cuts at: 5038 6750 5038 Size: 1712 5078 SexAI A'CCwGG_T Cuts at: 2926 2926 Size: 6790 SfcI C'TryA_G Cuts at: 826 1045 2177 2535 2797 3806 4138 5273 5954 Size: 219 1132 358 262 1009 332 1135 681 Cuts at: 5954 6145 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1132 1009 681 358 332 262 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6790 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6790 SmaI CCC'GGG Cuts at: 1872 4117 1872 Size: 2245 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3723 4894 5795 6057 820 Size: 197 926 1780 1171 901 262 1553 Fragments arranged by size: 1780 1553 1171 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4581 493 Size: 4088 2702 SpeI A'CTAG_T Cuts at: 152 3716 3758 152 Size: 3564 42 3184 Fragments arranged by size: 3564 3184 42 SphI G_CATG'C Cuts at: 1132 2975 4148 1132 Size: 1843 1173 3774 Fragments arranged by size: 3774 1843 1173 SspI AAT'ATT Cuts at: 5 52 4714 5 Size: 47 4662 2081 Fragments arranged by size: 4662 2081 47 StuI AGG'CCT Cuts at: 1517 3942 1517 Size: 2425 4365 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 2697 3690 3780 4071 4229 Size: 552 939 198 495 993 90 291 158 Cuts at: 4229 513 Size: 3074 Fragments arranged by size: 3074 993 939 552 495 291 198 158 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2882 4817 4834 4987 5172 5617 325 Size: 1255 1302 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1302 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5036 6748 96 Size: 274 80 33 51 1443 3059 1712 138 Fragments arranged by size: 3059 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3745 3777 6750 6782 3745 Size: 32 2973 32 3753 Fragments arranged by size: 3753 2973 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6790 XbaI T'CTAG_A Cuts at: 4126 4126 Size: 6790 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6790 XmaI C'CCGG_G Cuts at: 1870 4115 1870 Size: 2245 4545 XmnI GAAnn'nnTTC Cuts at: 3657 4919 3657 Size: 1262 5528 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4598 276 Size: 53 83 186 936 3064 2468 Fragments arranged by size: 3064 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI