Restriction map for
pFN21AA1321
(Linear) (Six-base) MAPSORT of: KIAA1321.seq Check: 530 from: 1 to: 2085
KIAA1321.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 392 2085
Size: 392 1693
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 956 1364 2085
Size: 424 532 408 721
Fragments arranged by size:
721 532 424 408
AfeI AGC'GCT
Cuts at: 0 1214 2085
Size: 1214 871
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1656 1688 2085
Size: 1656 32 397
Fragments arranged by size:
1656 397 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1267 1302 1387 1666 2085
Size: 1267 35 85 279 419
Fragments arranged by size:
1267 419 279 85 35
ApoI r'AATT_y
Cuts at: 0 456 2019 2085
Size: 456 1563 66
Fragments arranged by size:
1563 456 66
BanII G_rGCy'C
Cuts at: 0 1544 2085
Size: 1544 541
BbvCI CC'TCA_GC
Cuts at: 0 29 68 2085
Size: 29 39 2017
Fragments arranged by size:
2017 39 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 860 1015 1538 1577 1778 2085
Size: 860 155 523 39 201 307
Fragments arranged by size:
860 523 307 201 155 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 323 536 878 1271 1345 1720 2085
Size: 226 97 213 342 393 74 375 365
Fragments arranged by size:
393 375 365 342 226 213 97 74
Bpu10I CC'TnA_GC
Cuts at: 0 29 68 2085
Size: 29 39 2017
Fragments arranged by size:
2017 39 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1703 2085
Size: 1703 382
BsaI GGTCTCn'nnnn_
Cuts at: 0 736 2085
Size: 736 1349
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 224 254 2085
Size: 224 30 1831
Fragments arranged by size:
1831 224 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 2085
Size: 19 2066
BseYI C'CCAG_C
Cuts at: 0 203 1955 2085
Size: 203 1752 130
Fragments arranged by size:
1752 203 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 2085
Size: 739 1346
BsiHKAI G_wGCw'C
Cuts at: 0 1453 2085
Size: 1453 632
BsmI GAATG_Cn'
Cuts at: 0 1733 2085
Size: 1733 352
Bsp1286I G_dGCh'C
Cuts at: 0 1453 1544 2085
Size: 1453 91 541
Fragments arranged by size:
1453 541 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 888 1808 2085
Size: 179 709 920 277
Fragments arranged by size:
920 709 277 179
BsrBI CCG'CTC
Cuts at: 0 647 2085
Size: 647 1438
BssSI C'ACGA_G
Cuts at: 0 996 2085
Size: 996 1089
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1440 2085
Size: 1440 645
BstBI TT'CG_AA
Cuts at: 0 826 2085
Size: 826 1259
BstEII G'GTnAC_C
Cuts at: 0 1795 2085
Size: 1795 290
BstYI r'GATC_y
Cuts at: 0 637 1464 2034 2085
Size: 637 827 570 51
Fragments arranged by size:
827 637 570 51
Bsu36I CC'TnA_GG
Cuts at: 0 1659 1972 2085
Size: 1659 313 113
Fragments arranged by size:
1659 313 113
BtgI C'CryG_G
Cuts at: 0 2066 2085
Size: 2066 19
BtsI GCAGTG_nn'
Cuts at: 0 1050 1300 2085
Size: 1050 250 785
Fragments arranged by size:
1050 785 250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 548 583 2085
Size: 548 35 1502
Fragments arranged by size:
1502 548 35
DraI TTT'AAA
Cuts at: 0 323 1011 2085
Size: 323 688 1074
Fragments arranged by size:
1074 688 323
DraIII CAC_nnn'GTG
Cuts at: 0 1642 2085
Size: 1642 443
EaeI y'GGCC_r
Cuts at: 0 15 2085
Size: 15 2070
EarI CTCTTCn'nnn_
Cuts at: 0 1122 1344 2085
Size: 1122 222 741
Fragments arranged by size:
1122 741 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 323 424 536 878 956 1271 1345
Size: 226 97 101 112 342 78 315 74
Cuts at: 1345 1364 1720 2085
Size: 19 356 365
Fragments arranged by size:
365 356 342 315 226 112 101 97
78 74 19
EcoO109I rG'GnC_Cy
Cuts at: 0 366 1200 2085
Size: 366 834 885
Fragments arranged by size:
885 834 366
EcoRV GAT'ATC
Cuts at: 0 1503 2085
Size: 1503 582
HaeII r_GCGC'y
Cuts at: 0 1216 2085
Size: 1216 869
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 1456 1488 1701 1733 1851 1883
Size: 511 32 913 32 213 32 118 32
Cuts at: 1883 2085
Size: 202
Fragments arranged by size:
913 511 213 202 118 32 32 32
32
MslI CAynn'nnrTG
Cuts at: 0 1793 2085
Size: 1793 292
MspA1I CmG'CkG
Cuts at: 0 216 976 1387 2085
Size: 216 760 411 698
Fragments arranged by size:
760 698 411 216
NcoI C'CATG_G
Cuts at: 0 2066 2085
Size: 2066 19
NspI r_CATG'y
Cuts at: 0 970 2085
Size: 970 1115
PfoI T'CCnGG_A
Cuts at: 0 1361 2085
Size: 1361 724
PpuMI rG'GwC_Cy
Cuts at: 0 366 1200 2085
Size: 366 834 885
Fragments arranged by size:
885 834 366
PshAI GACnn'nnGTC
Cuts at: 0 748 1370 2085
Size: 748 622 715
Fragments arranged by size:
748 715 622
PsiI TTA'TAA
Cuts at: 0 1534 2085
Size: 1534 551
PstI C_TGCA'G
Cuts at: 0 176 1805 2085
Size: 176 1629 280
Fragments arranged by size:
1629 280 176
PvuII CAG'CTG
Cuts at: 0 976 1387 2085
Size: 976 411 698
Fragments arranged by size:
976 698 411
SexAI A'CCwGG_T
Cuts at: 0 921 2085
Size: 921 1164
SfcI C'TryA_G
Cuts at: 0 172 530 792 1801 2085
Size: 172 358 262 1009 284
Fragments arranged by size:
1009 358 284 262 172
SmlI C'TyrA_G
Cuts at: 0 1718 2085
Size: 1718 367
SpeI A'CTAG_T
Cuts at: 0 1711 1753 2085
Size: 1711 42 332
Fragments arranged by size:
1711 332 42
SphI G_CATG'C
Cuts at: 0 970 2085
Size: 970 1115
StuI AGG'CCT
Cuts at: 0 1937 2085
Size: 1937 148
StyI C'CwwG_G
Cuts at: 0 197 692 1685 1775 2066 2085
Size: 197 495 993 90 291 19
Fragments arranged by size:
993 495 291 197 90 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 877 2085
Size: 877 1208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1740 1772 2085
Size: 1740 32 313
Fragments arranged by size:
1740 313 32
XmnI GAAnn'nnTTC
Cuts at: 0 1652 2085
Size: 1652 433
Enzymes that do cut:
AccI AcuI AfeI AloI AlwNI ApoI BanII BbvCI
BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtsI CspCI DraI
DraIII EaeI EarI Eco57MI EcoO109I EcoRV HaeII Hin4I
MslI MspA1I NcoI NspI PfoI PpuMI PshAI PsiI
PstI PvuII SexAI SfcI SmlI SpeI SphI StuI
StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BstXI BstZ17I
BtgZI ClaI DrdI EagI EciI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI