(Circular) (Six-base) MAPSORT of: pFN21AA1273.seq Check: 7694 from: 1 to: 6511 pFN21AA1273 6511 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2732 4321 278 Size: 53 83 186 936 1196 1589 2468 Fragments arranged by size: 2468 1589 1196 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2649 2794 3854 1937 Size: 712 145 1060 4594 Fragments arranged by size: 4594 1060 712 145 Acc65I G'GTAC_C Cuts at: 3832 3832 Size: 6511 AclI AA'CG_TT Cuts at: 1563 4638 5011 1563 Size: 3075 373 3063 Fragments arranged by size: 3075 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 2525 3398 3602 4574 5958 6226 1632 Size: 689 204 873 204 972 1384 268 1917 Fragments arranged by size: 1917 1384 972 873 689 268 204 204 AfeI AGC'GCT Cuts at: 2285 2285 Size: 6511 AflII C'TTAA_G Cuts at: 820 1017 3347 820 Size: 197 2330 3984 Fragments arranged by size: 3984 2330 197 AflIII A'CryG_T Cuts at: 1203 5410 6387 1203 Size: 4207 977 1327 Fragments arranged by size: 4207 1327 977 AgeI A'CCGG_T Cuts at: 5342 5342 Size: 6511 AhdI GACnn_n'nnGTC Cuts at: 5240 5240 Size: 6511 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6511 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2759 1184 Size: 1575 4936 AlwNI CAG_nnn'CTG Cuts at: 4254 5826 4254 Size: 1572 4939 ApaI G_GGCC'C Cuts at: 2257 2257 Size: 6511 ApaLI G'TGCA_C Cuts at: 4568 5724 4568 Size: 1156 5355 ApoI r'AATT_y Cuts at: 1493 2329 3203 3820 4046 4198 6307 1493 Size: 836 874 617 226 152 2109 1697 Fragments arranged by size: 2109 1697 874 836 617 226 152 AseI AT'TA_AT Cuts at: 160 1032 5065 160 Size: 872 4033 1606 Fragments arranged by size: 4033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 AvaI C'yCGr_G Cuts at: 1870 1943 2445 3836 1870 Size: 73 502 1391 4545 Fragments arranged by size: 4545 1391 502 73 BamHI G'GATC_C Cuts at: 3841 4208 3841 Size: 367 6144 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 2760 2930 3372 3798 Size: 325 217 627 768 205 170 442 426 Cuts at: 3798 3832 5287 618 Size: 34 1455 1842 Fragments arranged by size: 1842 1455 768 627 442 426 325 217 205 170 34 BanII G_rGCy'C Cuts at: 1391 2257 3830 1391 Size: 866 1573 4072 Fragments arranged by size: 4072 1573 866 BbeI G_GCGC'C Cuts at: 3376 3376 Size: 6511 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2222 2945 928 Size: 971 323 723 4494 Fragments arranged by size: 4494 971 723 323 BbvCI CC'TCA_GC Cuts at: 2202 2202 Size: 6511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4702 4736 1903 Size: 34 50 34 2681 34 3678 Fragments arranged by size: 3678 2681 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4403 5613 4403 Size: 1210 5301 BclI T'GATC_A Cuts at: 1548 1548 Size: 6511 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2618 3078 3690 Size: 107 122 71 631 605 946 460 612 Cuts at: 3690 5122 136 Size: 1432 1525 Fragments arranged by size: 1525 1432 946 631 612 605 460 122 107 71 BglII A'GATC_T Cuts at: 6506 6506 Size: 6511 BlpI GC'TnA_GC Cuts at: 3459 3928 3459 Size: 469 6042 Bme1580I G_kGCm'C Cuts at: 1790 2050 2257 3740 3801 4572 5728 1790 Size: 260 207 1483 61 771 1156 2573 Fragments arranged by size: 2573 1483 1156 771 260 207 61 BmgBI CAC'GTC Cuts at: 1318 2806 3007 3271 1318 Size: 1488 201 264 4558 Fragments arranged by size: 4558 1488 264 201 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2251 3067 3382 Size: 416 53 83 399 24 834 816 315 Cuts at: 3382 3738 4242 5200 442 Size: 356 504 958 1753 Fragments arranged by size: 1753 958 834 816 504 416 399 356 315 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2768 2831 5171 1239 Size: 1529 63 2340 2579 Fragments arranged by size: 2579 2340 1529 63 Bpu10I CC'TnA_GC Cuts at: 2112 2202 2222 3234 2112 Size: 90 20 1012 5389 Fragments arranged by size: 5389 1012 90 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2159 4636 5501 5799 2159 Size: 2477 865 298 2871 Fragments arranged by size: 2871 2477 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 2429 3632 5174 882 Size: 617 726 204 1203 1542 2219 Fragments arranged by size: 2219 1542 1203 726 617 204 BsaAI yAC'GTr Cuts at: 493 1204 4302 493 Size: 711 3098 2702 Fragments arranged by size: 3098 2702 711 BsaBI GATnn'nnATC Cuts at: 6505 6505 Size: 6511 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2510 2729 3108 3373 Size: 53 83 186 936 977 219 379 265 Cuts at: 3373 3531 4318 4700 5335 275 Size: 158 787 382 635 1451 Fragments arranged by size: 1451 977 936 787 635 382 379 265 219 186 158 83 53 BsaWI w'CCGG_w Cuts at: 3623 4944 5342 5616 5763 6174 3623 Size: 1321 398 274 147 411 3960 Fragments arranged by size: 3960 1321 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 3425 3455 1178 Size: 30 165 30 675 30 1317 30 4234 Fragments arranged by size: 4234 1317 675 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2224 2243 2339 2360 2777 3149 3317 Size: 1033 5 19 96 21 417 372 168 Cuts at: 3317 1186 Size: 4380 Fragments arranged by size: 4380 1033 417 372 168 96 21 19 5 BseYI C'CCAG_C Cuts at: 2621 2701 2831 5714 6210 2621 Size: 80 130 2883 496 2922 Fragments arranged by size: 2922 2883 496 130 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2015 2096 2375 2951 3682 1163 Size: 573 279 81 279 576 731 3992 Fragments arranged by size: 3992 731 576 573 279 279 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3778 3782 3961 4722 5750 1540 Size: 275 186 1777 4 179 761 1028 2301 Fragments arranged by size: 2301 1777 1028 761 275 186 179 4 BsiHKAI G_wGCw'C Cuts at: 2143 2520 3413 3615 4572 4657 5728 2143 Size: 377 893 202 957 85 1071 2926 Fragments arranged by size: 2926 1071 957 893 377 202 85 BsmI GAATG_Cn' Cuts at: 2479 2668 4022 4115 2479 Size: 189 1354 93 4875 Fragments arranged by size: 4875 1354 189 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2720 2720 Size: 6511 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2143 2257 2520 3413 3615 3740 Size: 399 260 93 114 263 893 202 125 Cuts at: 3740 3801 3830 4572 4657 5728 1391 Size: 61 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 893 742 399 263 260 202 125 114 93 85 61 29 BspEI T'CCGG_A Cuts at: 3623 6174 3623 Size: 2551 3960 BspHI T'CATG_A Cuts at: 2991 4400 6130 2991 Size: 1409 1730 3372 Fragments arranged by size: 3372 1730 1409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2034 3297 3866 844 Size: 1190 1263 569 3489 Fragments arranged by size: 3489 1263 1190 569 BsrBI CCG'CTC Cuts at: 1394 1823 2089 2392 2467 3307 4398 1394 Size: 429 266 303 75 840 1091 3507 Fragments arranged by size: 3507 1091 840 429 303 266 75 BsrDI GCAATG_nn' Cuts at: 65 1248 5006 5180 65 Size: 1183 3758 174 1396 Fragments arranged by size: 3758 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2778 3072 3352 3689 5155 5342 1700 Size: 110 968 294 280 337 1466 187 2869 Fragments arranged by size: 2869 1466 968 337 294 280 187 110 BsrGI T'GTAC_A Cuts at: 96 2898 96 Size: 2802 3709 BssHII G'CGCG_C Cuts at: 1925 2450 2452 1925 Size: 525 2 5984 Fragments arranged by size: 5984 525 2 BssSI C'ACGA_G Cuts at: 4571 5583 4571 Size: 1012 5499 BstAPI GCAn_nnn'nTGC Cuts at: 794 2911 794 Size: 2117 4394 BstBI TT'CG_AA Cuts at: 5315 5315 Size: 6511 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6511 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6511 BstYI r'GATC_y Cuts at: 1972 2187 2496 3841 4208 4593 4610 6062 6148 Size: 215 309 1345 367 385 17 1452 86 Cuts at: 6148 6160 6506 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1345 385 367 346 309 215 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2163 2385 2613 3015 3483 3663 513 Size: 552 1098 222 228 402 468 180 3361 Fragments arranged by size: 3361 1098 552 468 402 228 222 180 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2842 487 Size: 683 56 1616 4156 Fragments arranged by size: 4156 1616 683 56 BtsI GCAGTG_nn' Cuts at: 2107 4027 4821 4841 2107 Size: 1920 794 20 3777 Fragments arranged by size: 3777 1920 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6476 DraI TTT'AAA Cuts at: 3815 3815 Size: 6511 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3275 5518 809 Size: 507 1959 2243 1802 Fragments arranged by size: 2243 1959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2680 3219 3354 Size: 54 1425 50 275 18 850 539 135 Cuts at: 3354 3465 3660 3779 3958 4847 6476 8 Size: 111 195 119 179 889 1629 43 Fragments arranged by size: 1629 1425 889 850 539 275 195 179 135 119 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3779 3958 1537 Size: 275 1967 179 4090 Fragments arranged by size: 4090 1967 275 179 EarI CTCTTCn'nnn_ Cuts at: 1353 3095 4441 1353 Size: 1742 1346 3423 Fragments arranged by size: 3423 1742 1346 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2489 3602 5098 5472 5618 387 Size: 1064 138 900 1113 1496 374 146 1280 Fragments arranged by size: 1496 1280 1113 1064 900 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2525 2768 2831 3398 3602 4574 Size: 393 689 204 243 63 567 204 972 Cuts at: 4574 5171 5958 6226 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 972 787 689 597 567 393 268 243 204 204 63 EcoNI CCTnn'n_nnAGG Cuts at: 2225 3515 2225 Size: 1290 5221 EcoO109I rG'GnC_Cy Cuts at: 2253 2893 3148 3508 3593 3743 2253 Size: 640 255 360 85 150 5021 Fragments arranged by size: 5021 640 360 255 150 85 EcoRI G'AATT_C Cuts at: 3820 4198 3820 Size: 378 6133 FspI TGC'GCA Cuts at: 5017 5017 Size: 6511 HaeII r_GCGC'y Cuts at: 1678 2287 2756 3316 3376 5658 1678 Size: 609 469 560 60 2282 2531 Fragments arranged by size: 2531 2282 609 560 469 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 3425 3457 5152 Size: 32 135 32 506 32 1315 32 1695 Cuts at: 5152 5184 5226 5258 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1695 1315 506 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2795 3855 4101 669 Size: 988 281 857 1060 246 3079 Fragments arranged by size: 3079 1060 988 857 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6511 HpaI GTT'AAC Cuts at: 4101 4101 Size: 6511 KasI G'GCGC_C Cuts at: 3372 3372 Size: 6511 KpnI G_GTAC'C Cuts at: 3836 3836 Size: 6511 MfeI C'AATT_G Cuts at: 4110 4110 Size: 6511 MluI A'CGCG_T Cuts at: 6387 6387 Size: 6511 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5625 5809 1305 Size: 4320 184 2007 Fragments arranged by size: 4320 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3221 3662 10 Size: 54 1425 343 1389 441 2859 Fragments arranged by size: 2859 1425 1389 441 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2415 2741 3269 4469 4828 4987 518 Size: 648 1249 326 528 1200 359 159 2042 Fragments arranged by size: 2042 1249 1200 648 528 359 326 159 MspA1I CmG'CkG Cuts at: 1606 2011 2126 3463 3927 4604 5752 5997 1606 Size: 405 115 1337 464 677 1148 245 2120 Fragments arranged by size: 2120 1337 1148 677 464 405 245 115 NaeI GCC'GGC Cuts at: 2780 3074 3354 3691 2780 Size: 294 280 337 5600 Fragments arranged by size: 5600 337 294 280 NarI GG'CG_CC Cuts at: 3373 3373 Size: 6511 NcoI C'CATG_G Cuts at: 513 1065 3015 513 Size: 552 1950 4009 Fragments arranged by size: 4009 1950 552 NdeI CA'TA_TG Cuts at: 387 3490 387 Size: 3103 3408 NgoMIV G'CCGG_C Cuts at: 2778 3072 3352 3689 2778 Size: 294 280 337 5600 Fragments arranged by size: 5600 337 294 280 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6511 NotI GC'GGCC_GC Cuts at: 3958 3958 Size: 6511 NspI r_CATG'y Cuts at: 1132 1229 3442 3869 5414 1132 Size: 97 2213 427 1545 2229 Fragments arranged by size: 2229 2213 1545 427 97 PasI CC'CwG_GG Cuts at: 1348 3740 3794 1348 Size: 2392 54 4065 Fragments arranged by size: 4065 2392 54 PciI A'CATG_T Cuts at: 5410 5410 Size: 6511 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6511 PfoI T'CCnGG_A Cuts at: 3716 3716 Size: 6511 PmeI GTTT'AAAC Cuts at: 3815 3815 Size: 6511 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4580 4612 6093 6125 4580 Size: 32 1481 32 4966 Fragments arranged by size: 4966 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3148 3508 3148 Size: 360 6151 PshAI GACnn'nnGTC Cuts at: 1613 2672 1613 Size: 1059 5452 PsiI TTA'TAA Cuts at: 4081 4081 Size: 6511 PspOMI G'GGCC_C Cuts at: 2253 2253 Size: 6511 PstI C_TGCA'G Cuts at: 830 2148 3863 830 Size: 1318 1715 3478 Fragments arranged by size: 3478 1715 1318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6511 PvuII CAG'CTG Cuts at: 2011 2126 3463 2011 Size: 115 1337 5059 Fragments arranged by size: 5059 1337 115 SalI G'TCGA_C Cuts at: 1936 2793 3853 1936 Size: 857 1060 4594 Fragments arranged by size: 4594 1060 857 SapI GCTCTTCn'nnn_ Cuts at: 3095 3095 Size: 6511 SbfI CC_TGCA'GG Cuts at: 3863 3863 Size: 6511 ScaI AGT'ACT Cuts at: 4759 6471 4759 Size: 1712 4799 SfcI C'TryA_G Cuts at: 826 1045 2144 3859 4994 5675 5866 826 Size: 219 1099 1715 1135 681 191 1471 Fragments arranged by size: 1715 1471 1135 1099 681 219 191 SfoI GGC'GCC Cuts at: 3374 3374 Size: 6511 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6511 SmaI CCC'GGG Cuts at: 1872 2447 3838 1872 Size: 575 1391 4545 Fragments arranged by size: 4545 1391 575 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 3347 4615 5516 5778 820 Size: 197 926 231 1173 1268 901 262 1553 Fragments arranged by size: 1553 1268 1173 926 901 262 231 197 SnaBI TAC'GTA Cuts at: 493 4302 493 Size: 3809 2702 SpeI A'CTAG_T Cuts at: 152 2038 152 Size: 1886 4625 SphI G_CATG'C Cuts at: 1132 3869 1132 Size: 2737 3774 SrfI GCCC'GGGC Cuts at: 2447 2447 Size: 6511 SspI AAT'ATT Cuts at: 5 52 4435 5 Size: 47 4383 2081 Fragments arranged by size: 4383 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6511 StyI C'CwwG_G Cuts at: 513 1065 2457 3015 3498 3631 3950 513 Size: 552 1392 558 483 133 319 3074 Fragments arranged by size: 3074 1392 558 552 483 319 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 4538 4555 4708 4893 5338 325 Size: 1255 873 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 873 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2898 4757 6469 Size: 274 80 33 51 1443 921 1859 1712 Cuts at: 6469 96 Size: 138 Fragments arranged by size: 1859 1712 1443 921 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3605 3637 3751 3783 6471 6503 3605 Size: 32 114 32 2688 32 3613 Fragments arranged by size: 3613 2688 114 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3506 1316 Size: 2190 4321 XbaI T'CTAG_A Cuts at: 3847 3847 Size: 6511 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6511 XmaI C'CCGG_G Cuts at: 1870 2445 3836 1870 Size: 575 1391 4545 Fragments arranged by size: 4545 1391 575 XmnI GAAnn'nnTTC Cuts at: 3170 4640 3170 Size: 1470 5041 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2730 4319 276 Size: 53 83 186 936 1196 1589 2468 Fragments arranged by size: 2468 1589 1196 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SwaI XcmI