Restriction map for
pFN21AA1238
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MroI MunI NarI NdeI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
XcmI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 479 CGCG CG^CG
AcsI 417 AAATTT [GA]^AATT[CT]
AflIII 612 ACGTGT A^C[GA][CT]GT
AhdI 36 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 182 GGATC GGATC(4/5)
ApoI 417 AAATTT [GA]^AATT[CT]
AspEI 36 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 714 GTGCTC G[AT]GC[AT]^C
AvaI 512 CTCGAG C^[CT]CG[GA]G
AvaII 1000 GGTCC G^G[AT]CC
BanI 643 GGCACC G^G[CT][GA]CC
BbsI 55 GAAGAC GAAGAC(2/6)
BcgI 414 CGAAAATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 390 GGATAC GTATCC(6/5)
BcnI 938 CCCGG CC^[GC]GG
BmyI 714 GTGCTC G[AGT]GC[ACT]^C
BpmI 451 CTGGAG CTGGAG(16/14)
BpuAI 55 GAAGAC GAAGAC(2/6)
BsaAI 611 TACGTG [CT]AC^GT[GA]
BseRI 106 GAGGAG GAGGAG(10/8)
BsiHKAI 714 GTGCTC G[AT]GC[AT]^C
BsmI 674 GAATGC GAATGC(1/-1)
BsoBI 512 CTCGAG C^[CT]CG[GA]G
Bsp1286I 714 GTGCTC G[AGT]GC[ACT]^C
BspMI 791 ACCTGC ACCTGC(4/8)
BsrFI 929 GCCGGC [GA]^CCGG[CT]
BstAPI 898 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 479 CGCG CG^CG
BstXI 235 CCAACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 929 GCCGGC [GA]^CCGG[CT]
DdeI 538 CTAAG C^T[ACGT]AG
Eam1105I 36 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 480 GCGGG CCCGC(4/6)
HaeII 561 AGCGCC [GA]GCGC^[CT]
HgaI 821 GCGTC GACGC(5/10)
HinfI 592 GAGTC G^A[ACGT]TC
MslI 618 CATTGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1123 CAGCTG C[AC]G^C[GT]G
MvnI 479 CGCG CG^CG
NaeI 929 GCCGGC GCC^GGC
NciI 938 CCCGG CC^[GC]GG
NcoI 372 CCATGG C^CATGG
NgoMIV 929 GCCGGC G^CCGGC
PaeR7I 512 CTCGAG C^TCGAG
PflMI 20 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 592 GAGTC GAGTC(4/5)
PpuMI 999 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1123 CAGCTG CAG^CTG
SfaNI 47 GCATC GCATC(5/9)
SpeI 982 ACTAGT A^CTAGT
Tsp509I 418 AATT ^AATT
Van91I 20 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 512 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 232 GTAC GT^AC
610 GTAC
BalI 369 TGGCCA TGG^CCA
408 TGGCCA
BsmAI 979 GAGAC GTCTC(1/5)
594 GTCTC
BsmFI 948 GTCCC GGGAC(10/14)
1001 GTCCC
CfoI 276 GCGC GCG^C
562 GCGC
Cfr13I 1000 GGTCC G^G[ACGT]CC
1104 GGGCC
DraII 999 GGGTCCC [GA]G^G[ACGT]CC[CT]
1103 GGGGCCT
DsaI 372 CCATGG C^C[GA][CT]GG
959 CCGTGG
EcoO109I 999 GGGTCCC [GA]G^G[ACGT]CC[CT]
1103 GGGGCCT
EcoT14I 241 CCTTGG C^C[AT][AT]GG
372 CCATGG
HhaI 276 GCGC GCG^C
562 GCGC
HinP1I 276 GCGC G^CGC
562 GCGC
HincII 1021 GTTGAC GT[CT]^[GA]AC
1051 GTTGAC
HindII 1021 GTTGAC GT[CT]^[GA]AC
1051 GTTGAC
MaeII 612 ACGT A^CGT
654 ACGT
MboII 55 GAAGA GAAGA(8/7)
288 GAAGA
MluNI 369 TGGCCA TGG^CCA
408 TGGCCA
MscI 369 TGGCCA TGG^CCA
408 TGGCCA
NheI 741 GCTAGC G^CTAGC
1128 GCTAGC
RsaI 232 GTAC GT^AC
610 GTAC
Sau96I 1000 GGTCC G^G[ACGT]CC
1104 GGGCC
SmlI 317 CTCAAG C^T[CT][GA]AG
512 CTCGAG
StyI 241 CCTTGG C^C[AT][AT]GG
372 CCATGG
TaqI 158 TCGA T^CGA
513 TCGA
Tsp45I 260 GTGAC ^GT[GC]AC
485 GTCAC
TthHB8I 158 TCGA T^CGA
513 TCGA
XbaI 282 TCTAGA T^CTAGA
876 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BbvI BfaI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeI MaeIII MboI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
Tru9I TseI TspRI