(Circular) (Six-base) MAPSORT of: pFN21AA1230.seq Check: 9580 from: 1 to: 7738 pFN21AA1230 7738 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5548 278 Size: 53 83 186 936 4012 2468 Fragments arranged by size: 4012 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4369 5081 1937 Size: 2432 712 4594 Fragments arranged by size: 4594 2432 712 Acc65I G'GTAC_C Cuts at: 5059 5059 Size: 7738 AclI AA'CG_TT Cuts at: 1563 5034 5865 6238 1563 Size: 3471 831 373 3063 Fragments arranged by size: 3471 3063 831 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 2342 2654 2810 2859 3434 3444 4155 Size: 614 96 312 156 49 575 10 711 Cuts at: 4155 4307 4953 5801 7185 7453 1632 Size: 152 646 848 1384 268 1917 Fragments arranged by size: 1917 1384 848 711 646 614 575 312 268 156 152 96 49 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7541 AflIII A'CryG_T Cuts at: 1203 2025 3828 4956 4974 6637 7614 1203 Size: 822 1803 1128 18 1663 977 1327 Fragments arranged by size: 1803 1663 1327 1128 977 822 18 AgeI A'CCGG_T Cuts at: 4875 6569 4875 Size: 1694 6044 AhdI GACnn_n'nnGTC Cuts at: 4279 6467 4279 Size: 2188 5550 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7738 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4035 1184 Size: 2851 4887 AlwNI CAG_nnn'CTG Cuts at: 3001 3251 4339 4694 5481 7053 3001 Size: 250 1088 355 787 1572 3686 Fragments arranged by size: 3686 1572 1088 787 355 250 ApaI G_GGCC'C Cuts at: 3879 3879 Size: 7738 ApaLI G'TGCA_C Cuts at: 3624 5795 6951 3624 Size: 2171 1156 4411 Fragments arranged by size: 4411 2171 1156 ApoI r'AATT_y Cuts at: 1493 3842 4000 4738 5047 5273 5425 7534 1493 Size: 2349 158 738 309 226 152 2109 1697 Fragments arranged by size: 2349 2109 1697 738 309 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 6292 160 Size: 872 5260 1606 Fragments arranged by size: 5260 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7738 AvaI C'yCGr_G Cuts at: 1870 1943 3894 5063 1870 Size: 73 1951 1169 4545 Fragments arranged by size: 4545 1951 1169 73 BamHI G'GATC_C Cuts at: 5068 5435 5068 Size: 367 7371 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3338 5059 6514 618 Size: 325 217 627 1551 1721 1455 1842 Fragments arranged by size: 1842 1721 1551 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2149 3475 3879 3992 4515 5057 1391 Size: 758 1326 404 113 523 542 4072 Fragments arranged by size: 4072 1326 758 542 523 404 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2169 3461 4600 5031 928 Size: 971 270 1292 1139 431 3635 Fragments arranged by size: 3635 1292 1139 971 431 270 BbvCI CC'TCA_GC Cuts at: 4632 4632 Size: 7738 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5929 5963 1903 Size: 34 3992 34 3678 Fragments arranged by size: 3992 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5630 6840 5630 Size: 1210 6528 BclI T'GATC_A Cuts at: 1548 1548 Size: 7738 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3520 6349 136 Size: 107 122 71 631 605 1848 2829 1525 Fragments arranged by size: 2829 1848 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2986 3569 3670 4057 7733 2986 Size: 583 101 387 3676 2991 Fragments arranged by size: 3676 2991 583 387 101 BlpI GC'TnA_GC Cuts at: 5155 5155 Size: 7738 Bme1580I G_kGCm'C Cuts at: 1790 3628 3879 5799 6955 1790 Size: 1838 251 1920 1156 2573 Fragments arranged by size: 2573 1920 1838 1156 251 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7738 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3159 4180 4213 Size: 416 53 83 399 24 1742 1021 33 Cuts at: 4213 4998 5469 6427 442 Size: 785 471 958 1753 Fragments arranged by size: 1753 1742 1021 958 785 471 416 399 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 4115 1056 Size: 3059 4679 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3132 3146 3726 4091 6398 1239 Size: 1893 14 580 365 2307 2579 Fragments arranged by size: 2579 2307 1893 580 365 14 Bpu10I CC'TnA_GC Cuts at: 2391 3291 4632 2391 Size: 900 1341 5497 Fragments arranged by size: 5497 1341 900 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2233 5863 6728 7026 2233 Size: 3630 865 298 2945 Fragments arranged by size: 3630 2945 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3897 6401 882 Size: 617 2398 2504 2219 Fragments arranged by size: 2504 2398 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5529 493 Size: 711 4325 2702 Fragments arranged by size: 4325 2702 711 BsaBI GATnn'nnATC Cuts at: 2307 7732 2307 Size: 5425 2313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5545 5927 6562 275 Size: 53 83 186 936 4012 382 635 1451 Fragments arranged by size: 4012 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4875 6171 6569 6843 6990 7401 4875 Size: 1296 398 274 147 411 5212 Fragments arranged by size: 5212 1296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2874 2904 3490 3520 1178 Size: 30 165 30 1471 30 586 30 5396 Fragments arranged by size: 5396 1471 586 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3082 3867 1186 Size: 1896 785 5057 Fragments arranged by size: 5057 1896 785 BseYI C'CCAG_C Cuts at: 6941 7437 6941 Size: 496 7242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 1163 Size: 573 399 6766 Fragments arranged by size: 6766 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5188 5949 6977 1540 Size: 275 186 3187 761 1028 2301 Fragments arranged by size: 3187 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2149 2436 3475 3628 3666 5799 5884 6955 2149 Size: 287 1039 153 38 2133 85 1071 2932 Fragments arranged by size: 2932 2133 1071 1039 287 153 85 38 BsmI GAATG_Cn' Cuts at: 2455 4084 4254 4925 5249 5342 2455 Size: 1629 170 671 324 93 4851 Fragments arranged by size: 4851 1629 671 324 170 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2149 2436 3475 3628 3666 3879 3992 Size: 399 359 287 1039 153 38 213 113 Cuts at: 3992 4515 5057 5799 5884 6955 1391 Size: 523 542 742 85 1071 2174 Fragments arranged by size: 2174 1071 1039 742 542 523 399 359 287 213 153 113 85 38 BspEI T'CCGG_A Cuts at: 7401 7401 Size: 7738 BspHI T'CATG_A Cuts at: 3771 5627 7357 3771 Size: 1856 1730 4152 Fragments arranged by size: 4152 1856 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 2175 3946 5093 844 Size: 1213 118 1771 1147 3489 Fragments arranged by size: 3489 1771 1213 1147 118 BsrBI CCG'CTC Cuts at: 1394 1823 5625 1394 Size: 429 3802 3507 Fragments arranged by size: 3802 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6233 6407 65 Size: 1183 4985 174 1396 Fragments arranged by size: 4985 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4875 6382 6569 1700 Size: 110 3065 1507 187 2869 Fragments arranged by size: 3065 2869 1507 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7738 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7738 BssSI C'ACGA_G Cuts at: 5798 6810 5798 Size: 1012 6726 BstAPI GCAn_nnn'nTGC Cuts at: 794 4475 794 Size: 3681 4057 BstBI TT'CG_AA Cuts at: 6542 6542 Size: 7738 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7738 BstXI CCAn_nnnn'nTGG Cuts at: 3403 3403 Size: 7738 BstYI r'GATC_y Cuts at: 1972 2223 2515 2986 3158 3569 3670 4057 4130 Size: 251 292 471 172 411 101 387 73 Cuts at: 4130 5068 5435 5820 5837 7289 7375 7387 7733 Size: 938 367 385 17 1452 86 12 346 Cuts at: 7733 1972 Size: 1977 Fragments arranged by size: 1977 1452 938 471 411 387 385 367 346 292 251 172 101 86 73 17 12 Bsu36I CC'TnA_GG Cuts at: 4340 4340 Size: 7738 BtgI C'CryG_G Cuts at: 513 1065 3534 3870 4458 4504 4659 513 Size: 552 2469 336 588 46 155 3592 Fragments arranged by size: 3592 2469 588 552 336 155 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4384 487 Size: 683 56 3158 3841 Fragments arranged by size: 3841 3158 683 56 BtsI GCAGTG_nn' Cuts at: 4501 5254 6048 6068 4501 Size: 753 794 20 6171 Fragments arranged by size: 6171 794 753 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7738 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7703 DraI TTT'AAA Cuts at: 2811 3595 4831 4981 5042 2811 Size: 784 1236 150 61 5507 Fragments arranged by size: 5507 1236 784 150 61 DraIII CAC_nnn'GTG Cuts at: 2158 2158 Size: 7738 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6745 809 Size: 507 5429 1802 Fragments arranged by size: 5429 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2709 3580 3692 Size: 54 1425 50 275 18 879 871 112 Cuts at: 3692 4027 4379 4602 5185 6074 7703 8 Size: 335 352 223 583 889 1629 43 Fragments arranged by size: 1629 1425 889 879 871 583 352 335 275 223 112 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5185 1537 Size: 275 3373 4090 Fragments arranged by size: 4090 3373 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2424 3060 3192 5668 1353 Size: 1071 636 132 2476 3423 Fragments arranged by size: 3423 2476 1071 636 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6325 6699 6845 387 Size: 1064 138 4736 374 146 1280 Fragments arranged by size: 4736 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2147 3473 2147 Size: 1326 6412 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2342 2654 2810 2859 3132 3146 Size: 393 614 96 312 156 49 273 14 Cuts at: 3146 3434 3444 3726 4091 4155 4307 4953 5801 Size: 288 10 282 365 64 152 646 848 Cuts at: 5801 6398 7185 7453 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 848 787 646 614 597 393 365 312 288 282 273 268 156 152 96 64 49 14 10 EcoNI CCTnn'n_nnAGG Cuts at: 4856 4856 Size: 7738 EcoO109I rG'GnC_Cy Cuts at: 3498 3513 3875 3876 4336 3498 Size: 15 362 1 460 6900 Fragments arranged by size: 6900 460 362 15 1 EcoRI G'AATT_C Cuts at: 3842 4000 5047 5425 3842 Size: 158 1047 378 6155 Fragments arranged by size: 6155 1047 378 158 FspI TGC'GCA Cuts at: 6244 6244 Size: 7738 HaeII r_GCGC'y Cuts at: 1678 6885 1678 Size: 5207 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2208 2240 2330 Size: 32 135 32 524 32 80 32 90 Cuts at: 2330 2362 3490 3522 3700 3718 3732 3750 3815 Size: 32 1128 32 178 18 14 18 65 Cuts at: 3815 3847 4265 4297 4417 4449 5012 5044 6379 Size: 32 418 32 120 32 563 32 1335 Cuts at: 6379 6411 6453 6485 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1335 1128 563 524 418 178 135 120 90 80 65 42 32 32 32 32 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2373 3285 5082 5328 669 Size: 988 281 435 912 1797 246 3079 Fragments arranged by size: 3079 1797 988 912 435 281 246 HindIII A'AGCT_T Cuts at: 748 2684 748 Size: 1936 5802 HpaI GTT'AAC Cuts at: 2373 5328 2373 Size: 2955 4783 KpnI G_GTAC'C Cuts at: 5063 5063 Size: 7738 MfeI C'AATT_G Cuts at: 3964 5337 3964 Size: 1373 6365 MluI A'CGCG_T Cuts at: 7614 7614 Size: 7738 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2022 2493 4167 6852 7036 1305 Size: 717 471 1674 2685 184 2007 Fragments arranged by size: 2685 2007 1674 717 471 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2711 4029 4604 10 Size: 54 1425 343 879 1318 575 3144 Fragments arranged by size: 3144 1425 1318 879 575 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3533 5696 6055 6214 518 Size: 648 2367 2163 359 159 2042 Fragments arranged by size: 2367 2163 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2558 2764 3872 5154 5831 6979 7224 1606 Size: 952 206 1108 1282 677 1148 245 2120 Fragments arranged by size: 2120 1282 1148 1108 952 677 245 206 NcoI C'CATG_G Cuts at: 513 1065 3534 4504 4659 513 Size: 552 2469 970 155 3592 Fragments arranged by size: 3592 2469 970 552 155 NdeI CA'TA_TG Cuts at: 387 387 Size: 7738 NheI G'CTAG_C Cuts at: 1052 4111 1052 Size: 3059 4679 NotI GC'GGCC_GC Cuts at: 5185 5185 Size: 7738 NspI r_CATG'y Cuts at: 1132 1229 2029 3832 4960 4978 5096 6641 1132 Size: 97 800 1803 1128 18 118 1545 2229 Fragments arranged by size: 2229 1803 1545 1128 800 118 97 18 PasI CC'CwG_GG Cuts at: 1348 3518 4126 1348 Size: 2170 608 4960 Fragments arranged by size: 4960 2170 608 PciI A'CATG_T Cuts at: 2025 3828 4956 4974 6637 2025 Size: 1803 1128 18 1663 3126 Fragments arranged by size: 3126 1803 1663 1128 18 PflMI CCAn_nnn'nTGG Cuts at: 1108 4126 1108 Size: 3018 4720 PmeI GTTT'AAAC Cuts at: 5042 5042 Size: 7738 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5807 5839 7320 7352 5807 Size: 32 1481 32 6193 Fragments arranged by size: 6193 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3498 4336 3498 Size: 838 6900 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7738 PsiI TTA'TAA Cuts at: 5308 5308 Size: 7738 PspOMI G'GGCC_C Cuts at: 3875 3875 Size: 7738 PstI C_TGCA'G Cuts at: 830 2038 2472 3390 5090 830 Size: 1208 434 918 1700 3478 Fragments arranged by size: 3478 1700 1208 918 434 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7738 PvuII CAG'CTG Cuts at: 2558 2558 Size: 7738 SacI G_AGCT'C Cuts at: 2149 3475 2149 Size: 1326 6412 SacII CC_GC'GG Cuts at: 3873 3873 Size: 7738 SalI G'TCGA_C Cuts at: 1936 5080 1936 Size: 3144 4594 SanDI GG'GwC_CC Cuts at: 3498 3498 Size: 7738 SapI GCTCTTCn'nnn_ Cuts at: 2424 3060 2424 Size: 636 7102 SbfI CC_TGCA'GG Cuts at: 5090 5090 Size: 7738 ScaI AGT'ACT Cuts at: 5986 7698 5986 Size: 1712 6026 SexAI A'CCwGG_T Cuts at: 2379 4834 2379 Size: 2455 5283 SfcI C'TryA_G Cuts at: 826 1045 2034 2468 2559 3386 5086 6221 6902 Size: 219 989 434 91 827 1700 1135 681 Cuts at: 6902 7093 826 Size: 191 1471 Fragments arranged by size: 1700 1471 1135 989 827 681 434 219 191 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7738 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7738 SmaI CCC'GGG Cuts at: 1872 5065 1872 Size: 3193 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2212 3894 5842 6743 7005 820 Size: 197 926 269 1682 1948 901 262 1553 Fragments arranged by size: 1948 1682 1553 926 901 269 262 197 SnaBI TAC'GTA Cuts at: 493 5529 493 Size: 5036 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7738 SphI G_CATG'C Cuts at: 1132 5096 1132 Size: 3964 3774 SspI AAT'ATT Cuts at: 5 52 2087 5662 5 Size: 47 2035 3575 2081 Fragments arranged by size: 3575 2081 2035 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7738 StyI C'CwwG_G Cuts at: 513 1065 3043 3334 3534 3948 4504 4659 5177 Size: 552 1978 291 200 414 556 155 518 Cuts at: 5177 513 Size: 3074 Fragments arranged by size: 3074 1978 556 552 518 414 291 200 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3157 4588 5765 5782 5935 6120 6565 Size: 1255 1577 1431 1177 17 153 185 445 Cuts at: 6565 325 Size: 1498 Fragments arranged by size: 1577 1498 1431 1255 1177 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2917 4678 5984 Size: 274 80 33 51 1443 940 1761 1306 Cuts at: 5984 7696 96 Size: 1712 138 Fragments arranged by size: 1761 1712 1443 1306 940 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3175 3207 7698 7730 3175 Size: 32 4491 32 3183 Fragments arranged by size: 4491 3183 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7738 XbaI T'CTAG_A Cuts at: 4060 4522 5074 4060 Size: 462 552 6724 Fragments arranged by size: 6724 552 462 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2704 3050 3151 3939 2704 Size: 346 101 788 6503 Fragments arranged by size: 6503 788 346 101 XhoI C'TCGA_G Cuts at: 1943 3894 1943 Size: 1951 5787 XmaI C'CCGG_G Cuts at: 1870 5063 1870 Size: 3193 4545 XmnI GAAnn'nnTTC Cuts at: 2259 2988 3447 5867 2259 Size: 729 459 2420 4130 Fragments arranged by size: 4130 2420 729 459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5546 276 Size: 53 83 186 936 4012 2468 Fragments arranged by size: 4012 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SfiI SfoI SrfI SwaI