Restriction map for
pFN21AA1216
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BbvCI
BcnI BfrI BglI BlnI BsaAI
BseAI BsiWI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
BstAPI BstEII BstPI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FauI
FseI FspI HindIII HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NciI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 3531 GACGTC GACGT^C
AccI 3742 GTATAC GT^[AC][GT]AC
AcyI 3531 GACGTC G[GA]^CG[CT]C
AocI 2245 CCTCAGG CC^T[ACGT]AGG
Aor51HI 884 AGCGCT AGC^GCT
BalI 2606 TGGCCA TGG^CCA
BclI 3452 TGATCA T^GATCA
BglII 405 AGATCT A^GATCT
BlpI 245 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 245 GCTTAGC GC^T[ACGT]AGC
BsaBI 775 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3531 GACGTC G[GA]^CG[CT]C
BsgI 3025 CTGCAC GTGCAG(16/14)
BsiEI 3112 CGACCG CG[GA][CT]^CG
BsmBI 4 GAGACG CGTCTC(1/5)
BsmFI 3254 GTCCC GGGAC(10/14)
BssSI 3164 CACGAG CACGAG(-5/-1)
Bst1107I 3742 GTATAC GTA^TAC
BstBI 25 TTCGAA TT^CGAA
BstZ17I 3742 GTATAC GTA^TAC
Bsu36I 2245 CCTCAGG CC^T[ACGT]AGG
CelII 245 GCTTAGC GC^T[ACGT]AGC
EaeI 2606 TGGCCA [CT]^GGCC[GA]
Eco47III 884 AGCGCT AGC^GCT
Eco81I 2245 CCTCAGG CC^T[ACGT]AGG
EcoNI 751 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3724 GAATTC G^AATTC
FbaI 3452 TGATCA T^GATCA
HaeII 884 AGCGCT [GA]GCGC^[CT]
HgaI 6 GACGC GACGC(5/10)
Hin1I 3531 GACGTC G[GA]^CG[CT]C
MamI 775 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3443 CAATTG C^AATTG
MluNI 2606 TGGCCA TGG^CCA
MscI 2606 TGGCCA TGG^CCA
MunI 3443 CAATTG C^AATTG
NspV 25 TTCGAA TT^CGAA
PflMI 906 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1191 GCTCTTC GCTCTTC(1/4)
ScaI 3699 AGTACT AGT^ACT
SfuI 25 TTCGAA TT^CGAA
SpeI 3222 ACTAGT A^CTAGT
StuI 759 AGGCCT AGG^CCT
SwaI 3860 ATTTAAAT ATTT^AAAT
Van91I 906 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 2811 CGCG CG^CG
3055 CGCG
AseI 2407 ATTAAT AT^TAAT
2592 ATTAAT
AsnI 2407 ATTAAT AT^TAAT
2592 ATTAAT
Asp700I 1188 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3697 GAAGTACTTC
BcgI 1935 CGAGAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3218 GCAGACTAGTCG
BsaWI 1829 ACCGGA [AT]^CCGG[AT]
2934 TCCGGT
Bsp1407I 468 TGTACA T^GTACA
2832 TGTACA
BsrGI 468 TGTACA T^GTACA
2832 TGTACA
BstF5I 1832 GGATG GGATG(2/0)
3434 GGATG
BstUI 2811 CGCG CG^CG
3055 CGCG
DsaI 932 CCATGG C^C[GA][CT]GG
1472 CCATGG
FokI 1832 GGATG GGATG(9/13)
3434 GGATG
HapII 1830 CCGG C^CGG
2935 CCGG
HincII 1254 GTCAAC GT[CT]^[GA]AC
3841 GTTGAC
HindII 1254 GTCAAC GT[CT]^[GA]AC
3841 GTTGAC
HpaII 1830 CCGG C^CGG
2935 CCGG
MaeII 3532 ACGT A^CGT
3622 ACGT
MspA1I 3299 CAGCTG C[AC]G^C[GT]G
4019 CAGCTG
MspI 1830 CCGG C^CGG
2935 CCGG
MvnI 2811 CGCG CG^CG
3055 CGCG
NcoI 932 CCATGG C^CATGG
1472 CCATGG
NdeI 770 CATATG CA^TATG
1051 CATATG
PshBI 2407 ATTAAT AT^TAAT
2592 ATTAAT
PvuII 3299 CAGCTG CAG^CTG
4019 CAGCTG
SspBI 468 TGTACA T^GTACA
2832 TGTACA
XmnI 1188 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3697 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BamHI
BanI BanII BbsI BbvI BciVI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PstI RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII