(Circular) (Six-base) MAPSORT of: pFN21AA1216.seq Check: 711 from: 1 to: 8809 pFN21AA1216 8809 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5540 6619 278 Size: 53 83 186 936 4004 1079 2468 Fragments arranged by size: 4004 2468 1079 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5748 6152 1937 Size: 3811 404 4594 Fragments arranged by size: 4594 3811 404 Acc65I G'GTAC_C Cuts at: 6130 6130 Size: 8809 AclI AA'CG_TT Cuts at: 1563 6936 7309 1563 Size: 5373 373 3063 Fragments arranged by size: 5373 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 2119 2601 2623 3537 4065 4136 5126 Size: 483 4 482 22 914 528 71 990 Cuts at: 5126 5721 6872 8256 8524 1632 Size: 595 1151 1384 268 1917 Fragments arranged by size: 1917 1384 1151 990 914 595 528 483 482 268 71 22 4 AfeI AGC'GCT Cuts at: 2891 2891 Size: 8809 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8612 AflIII A'CryG_T Cuts at: 1203 7708 8685 1203 Size: 6505 977 1327 Fragments arranged by size: 6505 1327 977 AgeI A'CCGG_T Cuts at: 7640 7640 Size: 8809 AhdI GACnn_n'nnGTC Cuts at: 7538 7538 Size: 8809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8809 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2166 2198 2166 Size: 32 8777 AlwNI CAG_nnn'CTG Cuts at: 2485 2558 3239 3874 4043 4115 4669 6552 8124 Size: 73 681 635 169 72 554 1883 1572 Cuts at: 8124 2485 Size: 3170 Fragments arranged by size: 3170 1883 1572 681 635 554 169 73 72 ApaLI G'TGCA_C Cuts at: 6866 8022 6866 Size: 1156 7653 ApoI r'AATT_y Cuts at: 1493 2229 3454 4548 4723 4991 5729 6118 6344 Size: 736 1225 1094 175 268 738 389 226 Cuts at: 6344 6496 8605 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1225 1094 738 736 389 268 226 175 152 AseI AT'TA_AT Cuts at: 160 1032 4413 4598 7363 160 Size: 872 3381 185 2765 1606 Fragments arranged by size: 3381 2765 1606 872 185 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8809 AvaI C'yCGr_G Cuts at: 1870 1943 6134 1870 Size: 73 4191 4545 Fragments arranged by size: 4545 4191 73 BamHI G'GATC_C Cuts at: 2734 4828 5878 6139 6506 2734 Size: 2094 1050 261 367 5037 Fragments arranged by size: 5037 2094 1050 367 261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2708 5652 6082 6130 7585 Size: 325 217 627 921 2944 430 48 1455 Cuts at: 7585 618 Size: 1842 Fragments arranged by size: 2944 1842 1455 921 627 430 325 217 48 BanII G_rGCy'C Cuts at: 1391 2465 3487 4358 5340 6128 1391 Size: 1074 1022 871 982 788 4072 Fragments arranged by size: 4072 1074 1022 982 871 788 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2675 4370 4877 5729 5981 928 Size: 971 776 1695 507 852 252 3756 Fragments arranged by size: 3756 1695 971 852 776 507 252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3929 3963 5212 5246 7000 7034 1903 Size: 34 1992 34 1249 34 1754 34 3678 Fragments arranged by size: 3678 1992 1754 1249 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2080 2192 2586 2626 3516 4954 6701 7911 2080 Size: 112 394 40 890 1438 1747 1210 2978 Fragments arranged by size: 2978 1747 1438 1210 890 394 112 40 BclI T'GATC_A Cuts at: 1548 5457 1548 Size: 3909 4900 BfrBI ATG'CAT Cuts at: 3443 3794 3841 4542 3443 Size: 351 47 701 7710 Fragments arranged by size: 7710 701 351 47 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7420 136 Size: 107 122 71 631 605 5748 1525 Fragments arranged by size: 5748 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2410 8804 2410 Size: 6394 2415 BlpI GC'TnA_GC Cuts at: 2251 6226 2251 Size: 3975 4834 Bme1580I G_kGCm'C Cuts at: 1790 6087 6870 8026 1790 Size: 4297 783 1156 2573 Fragments arranged by size: 4297 2573 1156 783 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3227 3250 6540 Size: 416 53 83 399 24 1810 23 3290 Cuts at: 6540 7498 442 Size: 958 1753 Fragments arranged by size: 3290 1810 1753 958 416 399 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8809 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3543 3887 3966 4188 3543 Size: 344 79 222 8164 Fragments arranged by size: 8164 344 222 79 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2924 3669 4976 5299 6045 7469 1239 Size: 1685 745 1307 323 746 1424 2579 Fragments arranged by size: 2579 1685 1424 1307 746 745 323 Bpu10I CC'TnA_GC Cuts at: 4199 4199 Size: 8809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 2992 3839 6934 7799 8097 2345 Size: 647 847 3095 865 298 3057 Fragments arranged by size: 3095 3057 865 847 647 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 2945 5049 6063 7472 882 Size: 617 780 666 2104 1014 1409 2219 Fragments arranged by size: 2219 2104 1409 1014 780 666 617 BsaAI yAC'GTr Cuts at: 493 1204 6600 493 Size: 711 5396 2702 Fragments arranged by size: 5396 2702 711 BsaBI GATnn'nnATC Cuts at: 2784 8803 2784 Size: 6019 2790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5537 6616 6998 7633 Size: 53 83 186 936 4004 1079 382 635 Cuts at: 7633 275 Size: 1451 Fragments arranged by size: 4004 1451 1079 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3834 4939 7242 7640 7914 8061 8472 3834 Size: 1105 2303 398 274 147 411 4171 Fragments arranged by size: 4171 2303 1105 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2897 2927 3474 3504 3798 Size: 30 165 30 1494 30 547 30 294 Cuts at: 3798 3828 4277 4307 1178 Size: 30 449 30 5680 Fragments arranged by size: 5680 1494 547 449 294 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2972 4164 4301 4304 4353 1186 Size: 1786 1192 137 3 49 5642 Fragments arranged by size: 5642 1786 1192 137 49 3 BseYI C'CCAG_C Cuts at: 5302 5528 8012 8508 5302 Size: 226 2484 496 5603 Fragments arranged by size: 5603 2484 496 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5015 1163 Size: 573 3279 4957 Fragments arranged by size: 4957 3279 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5120 6259 7020 8048 1540 Size: 275 186 3119 1139 761 1028 2301 Fragments arranged by size: 3119 2301 1139 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2465 3487 3947 4032 4737 5340 6870 6955 8026 Size: 1022 460 85 705 603 1530 85 1071 Cuts at: 8026 2465 Size: 3248 Fragments arranged by size: 3248 1530 1071 1022 705 603 460 85 85 BsmI GAATG_Cn' Cuts at: 4226 5170 5380 5449 6320 6413 4226 Size: 944 210 69 871 93 6622 Fragments arranged by size: 6622 944 871 210 93 69 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2003 Size: 8809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2465 3487 3947 4032 4358 4737 5340 Size: 399 675 1022 460 85 326 379 603 Cuts at: 5340 6087 6128 6870 6955 8026 1391 Size: 747 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 1022 747 742 675 603 460 399 379 326 85 85 41 BspEI T'CCGG_A Cuts at: 8472 8472 Size: 8809 BspHI T'CATG_A Cuts at: 2170 4161 4996 5497 6698 8428 2170 Size: 1991 835 501 1201 1730 2551 Fragments arranged by size: 2551 1991 1730 1201 835 501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6164 844 Size: 5320 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6696 1394 Size: 429 4873 3507 Fragments arranged by size: 4873 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2262 3282 3630 7304 7478 65 Size: 1183 1014 1020 348 3674 174 1396 Fragments arranged by size: 3674 1396 1183 1020 1014 348 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7453 7640 1700 Size: 110 5643 187 2869 Fragments arranged by size: 5643 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2473 4837 96 Size: 2377 2364 4068 Fragments arranged by size: 4068 2377 2364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8809 BssSI C'ACGA_G Cuts at: 5169 6869 7881 5169 Size: 1700 1012 6097 Fragments arranged by size: 6097 1700 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8809 BstBI TT'CG_AA Cuts at: 2031 7613 2031 Size: 5582 3227 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8809 BstXI CCAn_nnnn'nTGG Cuts at: 2848 4459 4737 4954 5759 2848 Size: 1611 278 217 805 5898 Fragments arranged by size: 5898 1611 805 278 217 BstYI r'GATC_y Cuts at: 1972 2410 2734 2785 2920 3557 3801 4828 4842 Size: 438 324 51 135 637 244 1027 14 Cuts at: 4842 5878 6139 6506 6891 6908 8360 8446 8458 Size: 1036 261 367 385 17 1452 86 12 Cuts at: 8458 8804 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1036 1027 637 438 385 367 346 324 261 244 135 86 51 17 14 12 BstZ17I GTA'TAC Cuts at: 5749 5749 Size: 8809 Bsu36I CC'TnA_GG Cuts at: 4251 4251 Size: 8809 BtgI C'CryG_G Cuts at: 513 1065 2004 2937 3477 513 Size: 552 939 933 540 5845 Fragments arranged by size: 5845 939 933 552 540 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8070 Fragments arranged by size: 8070 683 56 BtsI GCAGTG_nn' Cuts at: 3945 5463 5583 6036 6325 7119 7139 3945 Size: 1518 120 453 289 794 20 5615 Fragments arranged by size: 5615 1518 794 453 289 120 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2975 3010 529 Size: 35 2411 35 6328 Fragments arranged by size: 6328 2411 35 35 DraI TTT'AAA Cuts at: 2362 2871 5868 5979 6113 2362 Size: 509 2997 111 134 5058 Fragments arranged by size: 5058 2997 509 134 111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7816 809 Size: 507 6500 1802 Fragments arranged by size: 6500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4611 6256 7145 Size: 54 1425 50 275 18 2781 1645 889 Cuts at: 7145 8774 8 Size: 1629 43 Fragments arranged by size: 2781 1645 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6256 1537 Size: 275 4444 4090 Fragments arranged by size: 4444 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2055 2751 3075 3203 6739 1353 Size: 675 27 696 324 128 3536 3423 Fragments arranged by size: 3536 3423 696 675 324 128 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5558 7396 7770 7916 387 Size: 1064 138 3969 1838 374 146 1280 Fragments arranged by size: 3969 1838 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2463 3485 5338 2463 Size: 1022 1853 5934 Fragments arranged by size: 5934 1853 1022 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2119 2601 2623 2924 3537 3669 Size: 393 483 4 482 22 301 613 132 Cuts at: 3669 4065 4136 4976 5126 5299 5721 6045 6872 Size: 396 71 840 150 173 422 324 827 Cuts at: 6872 7469 8256 8524 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 840 827 787 613 597 483 482 422 396 393 324 301 268 173 150 132 71 22 4 EcoNI CCTnn'n_nnAGG Cuts at: 2760 2760 Size: 8809 EcoO109I rG'GnC_Cy Cuts at: 2130 4455 4810 2130 Size: 2325 355 6129 Fragments arranged by size: 6129 2325 355 EcoRI G'AATT_C Cuts at: 5729 6118 6496 5729 Size: 389 378 8042 Fragments arranged by size: 8042 389 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2250 2606 4166 5160 5424 5726 2250 Size: 356 1560 994 264 302 5333 Fragments arranged by size: 5333 1560 994 356 302 264 FspI TGC'GCA Cuts at: 7315 7315 Size: 8809 HaeII r_GCGC'y Cuts at: 1678 2893 7956 1678 Size: 1215 5063 2531 Fragments arranged by size: 5063 2531 1215 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4587 4619 4834 4866 7450 Size: 32 135 32 3015 32 215 32 2584 Cuts at: 7450 7482 7524 7556 1373 Size: 32 42 32 2626 Fragments arranged by size: 3015 2626 2584 215 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3261 5848 6153 6399 669 Size: 988 281 1323 2587 305 246 3079 Fragments arranged by size: 3079 2587 1323 988 305 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8809 HpaI GTT'AAC Cuts at: 6399 6399 Size: 8809 KpnI G_GTAC'C Cuts at: 6134 6134 Size: 8809 MfeI C'AATT_G Cuts at: 5448 6408 5448 Size: 960 7849 MluI A'CGCG_T Cuts at: 8685 8685 Size: 8809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5740 7923 8107 1305 Size: 4435 2183 184 2007 Fragments arranged by size: 4435 2183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4613 10 Size: 54 1425 343 2781 4206 Fragments arranged by size: 4206 2781 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2081 3793 3933 5037 6767 7126 7285 Size: 648 915 1712 140 1104 1730 359 159 Cuts at: 7285 518 Size: 2042 Fragments arranged by size: 2042 1730 1712 1104 915 648 359 159 140 MspA1I CmG'CkG Cuts at: 1606 5306 6026 6225 6902 8050 8295 1606 Size: 3700 720 199 677 1148 245 2120 Fragments arranged by size: 3700 2120 1148 720 677 245 199 NcoI C'CATG_G Cuts at: 513 1065 2004 2937 3477 513 Size: 552 939 933 540 5845 Fragments arranged by size: 5845 939 933 552 540 NdeI CA'TA_TG Cuts at: 387 2776 3057 387 Size: 2389 281 6139 Fragments arranged by size: 6139 2389 281 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8809 NotI GC'GGCC_GC Cuts at: 6256 6256 Size: 8809 NsiI A_TGCA'T Cuts at: 3445 3796 3843 4544 3445 Size: 351 47 701 7710 Fragments arranged by size: 7710 701 351 47 NspI r_CATG'y Cuts at: 1132 1229 2080 3794 5645 5836 6167 7712 1132 Size: 97 851 1714 1851 191 331 1545 2229 Fragments arranged by size: 2229 1851 1714 1545 851 331 191 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8809 PciI A'CATG_T Cuts at: 7708 7708 Size: 8809 PflMI CCAn_nnn'nTGG Cuts at: 1108 2917 1108 Size: 1809 7000 PfoI T'CCnGG_A Cuts at: 3585 3585 Size: 8809 PmeI GTTT'AAAC Cuts at: 6113 6113 Size: 8809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6878 6910 8391 8423 6878 Size: 32 1481 32 7264 Fragments arranged by size: 7264 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8809 PsiI TTA'TAA Cuts at: 3962 6379 3962 Size: 2417 6392 PstI C_TGCA'G Cuts at: 830 2022 3381 3901 6031 6161 830 Size: 1192 1359 520 2130 130 3478 Fragments arranged by size: 3478 2130 1359 1192 520 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8809 PvuII CAG'CTG Cuts at: 5306 6026 5306 Size: 720 8089 SacI G_AGCT'C Cuts at: 2465 3487 5340 2465 Size: 1022 1853 5934 Fragments arranged by size: 5934 1853 1022 SalI G'TCGA_C Cuts at: 1936 6151 1936 Size: 4215 4594 SapI GCTCTTCn'nnn_ Cuts at: 3203 3203 Size: 8809 SbfI CC_TGCA'GG Cuts at: 6161 6161 Size: 8809 ScaI AGT'ACT Cuts at: 5706 7057 8769 5706 Size: 1351 1712 5746 Fragments arranged by size: 5746 1712 1351 SfcI C'TryA_G Cuts at: 826 1045 2018 2677 3332 3377 3897 4220 4426 Size: 219 973 659 655 45 520 323 206 Cuts at: 4426 5715 6027 6157 7292 7973 8164 826 Size: 1289 312 130 1135 681 191 1471 Fragments arranged by size: 1471 1289 1135 973 681 659 655 520 323 312 219 206 191 130 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8809 SmaI CCC'GGG Cuts at: 1872 6136 1872 Size: 4264 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2324 3007 3818 6913 7814 8076 Size: 197 926 381 683 811 3095 901 262 Cuts at: 8076 820 Size: 1553 Fragments arranged by size: 3095 1553 926 901 811 683 381 262 197 SnaBI TAC'GTA Cuts at: 493 6600 493 Size: 6107 2702 SpeI A'CTAG_T Cuts at: 152 5227 152 Size: 5075 3734 SphI G_CATG'C Cuts at: 1132 6167 1132 Size: 5035 3774 SspI AAT'ATT Cuts at: 5 52 6733 5 Size: 47 6681 2081 Fragments arranged by size: 6681 2081 47 StuI AGG'CCT Cuts at: 1517 2766 1517 Size: 1249 7560 StyI C'CwwG_G Cuts at: 513 1065 2004 2937 3477 3678 4074 4458 5183 Size: 552 939 933 540 201 396 384 725 Cuts at: 5183 5346 6248 513 Size: 163 902 3074 Fragments arranged by size: 3074 939 933 902 725 552 540 396 384 201 163 SwaI ATTT'AAAT Cuts at: 5868 5868 Size: 8809 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2299 2969 5741 5876 6836 6853 7006 Size: 1255 719 670 2772 135 960 17 153 Cuts at: 7006 7191 7636 325 Size: 185 445 1498 Fragments arranged by size: 2772 1498 1255 960 719 670 445 185 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2473 4837 5704 Size: 274 80 33 51 1443 496 2364 867 Cuts at: 5704 7055 8767 96 Size: 1351 1712 138 Fragments arranged by size: 2364 1712 1443 1351 867 496 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8769 8801 8769 Size: 32 8777 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8809 XbaI T'CTAG_A Cuts at: 6145 6145 Size: 8809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3484 3676 5093 5274 5481 3484 Size: 192 1417 181 207 6812 Fragments arranged by size: 6812 1417 207 192 181 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8809 XmaI C'CCGG_G Cuts at: 1870 6134 1870 Size: 4264 4545 XmnI GAAnn'nnTTC Cuts at: 3197 5706 6938 3197 Size: 2509 1232 5068 Fragments arranged by size: 5068 2509 1232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5538 6617 276 Size: 53 83 186 936 4004 1079 2468 Fragments arranged by size: 4004 2468 1079 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI