Restriction map for
pFN21AA1216
(Linear) (Six-base) MAPSORT of: KIAA1216.seq Check: 3115 from: 1 to: 4104
KIAA1216.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AatII G_ACGT'C
Cuts at: 0 3535 4104
Size: 3535 569
AccI GT'mk_AC
Cuts at: 0 3743 4104
Size: 3743 361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 114 596 618 1532 2060 2131 3121
Size: 110 4 482 22 914 528 71 990
Cuts at: 3121 3716 4104
Size: 595 388
Fragments arranged by size:
990 914 595 528 482 388 110 71
22 4
AfeI AGC'GCT
Cuts at: 0 886 4104
Size: 886 3218
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 161 193 4104
Size: 161 32 3911
Fragments arranged by size:
3911 161 32
AlwNI CAG_nnn'CTG
Cuts at: 0 480 553 1234 1869 2038 2110 2664 4104
Size: 480 73 681 635 169 72 554 1440
Fragments arranged by size:
1440 681 635 554 480 169 73 72
ApoI r'AATT_y
Cuts at: 0 224 1449 2543 2718 2986 3724 4104
Size: 224 1225 1094 175 268 738 380
Fragments arranged by size:
1225 1094 738 380 268 224 175
AseI AT'TA_AT
Cuts at: 0 2408 2593 4104
Size: 2408 185 1511
Fragments arranged by size:
2408 1511 185
BamHI G'GATC_C
Cuts at: 0 729 2823 3873 4104
Size: 729 2094 1050 231
Fragments arranged by size:
2094 1050 729 231
BanI G'GyrC_C
Cuts at: 0 703 3647 4077 4104
Size: 703 2944 430 27
Fragments arranged by size:
2944 703 430 27
BanII G_rGCy'C
Cuts at: 0 460 1482 2353 3335 4104
Size: 460 1022 871 982 769
Fragments arranged by size:
1022 982 871 769 460
BbsI GAAGACnn'nnnn_
Cuts at: 0 670 2365 2872 3724 3976 4104
Size: 670 1695 507 852 252 128
Fragments arranged by size:
1695 852 670 507 252 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1924 1958 3207 3241 4104
Size: 1924 34 1249 34 863
Fragments arranged by size:
1924 1249 863 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 75 187 581 621 1511 2949 4104
Size: 75 112 394 40 890 1438 1155
Fragments arranged by size:
1438 1155 890 394 112 75 40
BclI T'GATC_A
Cuts at: 0 3452 4104
Size: 3452 652
BfrBI ATG'CAT
Cuts at: 0 1438 1789 1836 2537 4104
Size: 1438 351 47 701 1567
Fragments arranged by size:
1567 1438 701 351 47
BglII A'GATC_T
Cuts at: 0 405 4104
Size: 405 3699
BlpI GC'TnA_GC
Cuts at: 0 246 4104
Size: 246 3858
Bme1580I G_kGCm'C
Cuts at: 0 4082 4104
Size: 4082 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 1222 1245 4104
Size: 1222 23 2859
Fragments arranged by size:
2859 1222 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1538 1882 1961 2183 4104
Size: 1538 344 79 222 1921
Fragments arranged by size:
1921 1538 344 222 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1664 2971 3294 4040 4104
Size: 919 745 1307 323 746 64
Fragments arranged by size:
1307 919 746 745 323 64
Bpu10I CC'TnA_GC
Cuts at: 0 2194 4104
Size: 2194 1910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 987 1834 4104
Size: 340 647 847 2270
Fragments arranged by size:
2270 847 647 340
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 940 3044 4058 4104
Size: 274 666 2104 1014 46
Fragments arranged by size:
2104 1014 666 274 46
BsaBI GATnn'nnATC
Cuts at: 0 779 4104
Size: 779 3325
BsaHI Gr'CG_yC
Cuts at: 0 3532 4104
Size: 3532 572
BsaWI w'CCGG_w
Cuts at: 0 1829 2934 4104
Size: 1829 1105 1170
Fragments arranged by size:
1829 1170 1105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 892 922 1469 1499 1793 1823 2272 2302
Size: 892 30 547 30 294 30 449 30
Cuts at: 2302 4104
Size: 1802
Fragments arranged by size:
1802 892 547 449 294 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 967 2159 2296 2299 2348 4104
Size: 967 1192 137 3 49 1756
Fragments arranged by size:
1756 1192 967 137 49 3
BseYI C'CCAG_C
Cuts at: 0 3297 3523 4104
Size: 3297 226 581
Fragments arranged by size:
3297 581 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3010 4104
Size: 3010 1094
BsiEI CG_ry'CG
Cuts at: 0 3115 4104
Size: 3115 989
BsiHKAI G_wGCw'C
Cuts at: 0 460 1482 1942 2027 2732 3335 4104
Size: 460 1022 460 85 705 603 769
Fragments arranged by size:
1022 769 705 603 460 460 85
BsmI GAATG_Cn'
Cuts at: 0 2221 3165 3375 3444 4104
Size: 2221 944 210 69 660
Fragments arranged by size:
2221 944 660 210 69
Bsp1286I G_dGCh'C
Cuts at: 0 460 1482 1942 2027 2353 2732 3335 4082
Size: 460 1022 460 85 326 379 603 747
Cuts at: 4082 4104
Size: 22
Fragments arranged by size:
1022 747 603 460 460 379 326 85
22
BspHI T'CATG_A
Cuts at: 0 165 2156 2991 3492 4104
Size: 165 1991 835 501 612
Fragments arranged by size:
1991 835 612 501 165
BsrDI GCAATG_nn'
Cuts at: 0 257 1277 1625 4104
Size: 257 1020 348 2479
Fragments arranged by size:
2479 1020 348 257
BsrGI T'GTAC_A
Cuts at: 0 468 2832 4104
Size: 468 2364 1272
Fragments arranged by size:
2364 1272 468
BssSI C'ACGA_G
Cuts at: 0 3164 4104
Size: 3164 940
BstBI TT'CG_AA
Cuts at: 0 26 4104
Size: 26 4078
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 2454 2732 2949 3754 4104
Size: 843 1611 278 217 805 350
Fragments arranged by size:
1611 843 805 350 278 217
BstYI r'GATC_y
Cuts at: 0 405 729 780 915 1552 1796 2823 2837
Size: 405 324 51 135 637 244 1027 14
Cuts at: 2837 3873 4104
Size: 1036 231
Fragments arranged by size:
1036 1027 637 405 324 244 231 135
51 14
BstZ17I GTA'TAC
Cuts at: 0 3744 4104
Size: 3744 360
Bsu36I CC'TnA_GG
Cuts at: 0 2246 4104
Size: 2246 1858
BtgI C'CryG_G
Cuts at: 0 932 1472 4104
Size: 932 540 2632
Fragments arranged by size:
2632 932 540
BtsI GCAGTG_nn'
Cuts at: 0 1940 3458 3578 4031 4104
Size: 1940 1518 120 453 73
Fragments arranged by size:
1940 1518 453 120 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 970 1005 4104
Size: 970 35 3099
Fragments arranged by size:
3099 970 35
DraI TTT'AAA
Cuts at: 0 357 866 3863 3974 4104
Size: 357 509 2997 111 130
Fragments arranged by size:
2997 509 357 130 111
EaeI y'GGCC_r
Cuts at: 0 2606 4104
Size: 2606 1498
EarI CTCTTCn'nnn_
Cuts at: 0 23 50 746 1070 1198 4104
Size: 23 27 696 324 128 2906
Fragments arranged by size:
2906 696 324 128 27 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3553 4104
Size: 3553 551
EcoICRI GAG'CTC
Cuts at: 0 458 1480 3333 4104
Size: 458 1022 1853 771
Fragments arranged by size:
1853 1022 771 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 114 596 618 919 1532 1664 2060
Size: 110 4 482 22 301 613 132 396
Cuts at: 2060 2131 2971 3121 3294 3716 4040 4104
Size: 71 840 150 173 422 324 64
Fragments arranged by size:
840 613 482 422 396 324 301 173
150 132 110 71 64 22 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 755 4104
Size: 755 3349
EcoO109I rG'GnC_Cy
Cuts at: 0 125 2450 2805 4104
Size: 125 2325 355 1299
Fragments arranged by size:
2325 1299 355 125
EcoRI G'AATT_C
Cuts at: 0 3724 4104
Size: 3724 380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 245 601 2161 3155 3419 3721 4104
Size: 245 356 1560 994 264 302 383
Fragments arranged by size:
1560 994 383 356 302 264 245
HaeII r_GCGC'y
Cuts at: 0 888 4104
Size: 888 3216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2582 2614 2829 2861 4104
Size: 2582 32 215 32 1243
Fragments arranged by size:
2582 1243 215 32 32
HincII GTy'rAC
Cuts at: 0 1256 3843 4104
Size: 1256 2587 261
Fragments arranged by size:
2587 1256 261
MfeI C'AATT_G
Cuts at: 0 3443 4104
Size: 3443 661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3735 4104
Size: 3735 369
MscI TGG'CCA
Cuts at: 0 2608 4104
Size: 2608 1496
MslI CAynn'nnrTG
Cuts at: 0 76 1788 1928 3032 4104
Size: 76 1712 140 1104 1072
Fragments arranged by size:
1712 1104 1072 140 76
MspA1I CmG'CkG
Cuts at: 0 3301 4021 4104
Size: 3301 720 83
Fragments arranged by size:
3301 720 83
NcoI C'CATG_G
Cuts at: 0 932 1472 4104
Size: 932 540 2632
Fragments arranged by size:
2632 932 540
NdeI CA'TA_TG
Cuts at: 0 771 1052 4104
Size: 771 281 3052
Fragments arranged by size:
3052 771 281
NsiI A_TGCA'T
Cuts at: 0 1440 1791 1838 2539 4104
Size: 1440 351 47 701 1565
Fragments arranged by size:
1565 1440 701 351 47
NspI r_CATG'y
Cuts at: 0 75 1789 3640 3831 4104
Size: 75 1714 1851 191 273
Fragments arranged by size:
1851 1714 273 191 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 912 4104
Size: 912 3192
PfoI T'CCnGG_A
Cuts at: 0 1580 4104
Size: 1580 2524
PsiI TTA'TAA
Cuts at: 0 1957 4104
Size: 1957 2147
PstI C_TGCA'G
Cuts at: 0 17 1376 1896 4026 4104
Size: 17 1359 520 2130 78
Fragments arranged by size:
2130 1359 520 78 17
PvuII CAG'CTG
Cuts at: 0 3301 4021 4104
Size: 3301 720 83
Fragments arranged by size:
3301 720 83
SacI G_AGCT'C
Cuts at: 0 460 1482 3335 4104
Size: 460 1022 1853 769
Fragments arranged by size:
1853 1022 769 460
SapI GCTCTTCn'nnn_
Cuts at: 0 1198 4104
Size: 1198 2906
ScaI AGT'ACT
Cuts at: 0 3701 4104
Size: 3701 403
SfcI C'TryA_G
Cuts at: 0 13 672 1327 1372 1892 2215 2421 3710
Size: 13 659 655 45 520 323 206 1289
Cuts at: 3710 4022 4104
Size: 312 82
Fragments arranged by size:
1289 659 655 520 323 312 206 82
45 13
SmlI C'TyrA_G
Cuts at: 0 319 1002 1813 4104
Size: 319 683 811 2291
Fragments arranged by size:
2291 811 683 319
SpeI A'CTAG_T
Cuts at: 0 3222 4104
Size: 3222 882
StuI AGG'CCT
Cuts at: 0 761 4104
Size: 761 3343
StyI C'CwwG_G
Cuts at: 0 932 1472 1673 2069 2453 3178 3341 4104
Size: 932 540 201 396 384 725 163 763
Fragments arranged by size:
932 763 725 540 396 384 201 163
SwaI ATTT'AAAT
Cuts at: 0 3863 4104
Size: 3863 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 294 964 3736 3871 4104
Size: 294 670 2772 135 233
Fragments arranged by size:
2772 670 294 233 135
TatI w'GTAC_w
Cuts at: 0 468 2832 3699 4104
Size: 468 2364 867 405
Fragments arranged by size:
2364 867 468 405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1479 1671 3088 3269 3476 4104
Size: 1479 192 1417 181 207 628
Fragments arranged by size:
1479 1417 628 207 192 181
XmnI GAAnn'nnTTC
Cuts at: 0 1192 3701 4104
Size: 1192 2509 403
Fragments arranged by size:
2509 1192 403
ZraI GAC'GTC
Cuts at: 0 3533 4104
Size: 3533 571
Enzymes that do cut:
AatII AccI AcuI AfeI AloI AlwNI ApoI AseI
BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BssSI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI
DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PflMI PfoI
PsiI PstI PvuII SacI SapI ScaI SfcI SmlI
SpeI StuI StyI SwaI TaqII TatI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BbeI BbvCI BglI BmgBI BmtI BsaAI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BssHII BstAPI BstEII BtgZI
ClaI DraIII DrdI EagI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI TstI Tth111I
XbaI XhoI XmaI