(Circular) (Six-base) MAPSORT of: pFN21AA1153.seq Check: 4550 from: 1 to: 5905 pFN21AA1153 5905 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2224 3248 1937 Size: 287 1024 4594 Fragments arranged by size: 4594 1024 287 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2817 3152 3968 5352 5620 1632 Size: 1185 335 816 1384 268 1917 Fragments arranged by size: 1917 1384 1185 816 335 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 2627 4804 5781 1203 Size: 1424 2177 977 1327 Fragments arranged by size: 2177 1424 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3133 1184 Size: 1949 3956 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2697 2729 2697 Size: 32 5873 AlwNI CAG_nnn'CTG Cuts at: 3648 5220 3648 Size: 1572 4333 ApaI G_GGCC'C Cuts at: 2033 2033 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2423 2771 3214 3440 3592 5701 1493 Size: 930 348 443 226 152 2109 1697 Fragments arranged by size: 2109 1697 930 443 348 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3226 4681 618 Size: 325 217 627 1439 1455 1842 Fragments arranged by size: 1842 1455 1439 627 325 217 BanII G_rGCy'C Cuts at: 1391 2015 2033 3163 3224 1391 Size: 624 18 1130 61 4072 Fragments arranged by size: 4072 1130 624 61 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2789 2919 2958 928 Size: 971 890 130 39 3875 Fragments arranged by size: 3875 971 890 130 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2033 2279 3966 5122 1790 Size: 243 246 1687 1156 2573 Fragments arranged by size: 2573 1687 1156 246 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3636 4594 442 Size: 416 53 83 399 24 2219 958 1753 Fragments arranged by size: 2219 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2721 2900 4565 1239 Size: 1482 179 1665 2579 Fragments arranged by size: 2579 1665 1482 179 Bpu10I CC'TnA_GC Cuts at: 2014 2014 Size: 5905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2310 2964 4030 4895 5193 2310 Size: 654 1066 865 298 3022 Fragments arranged by size: 3022 1066 865 654 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2046 4568 882 Size: 617 547 2522 2219 Fragments arranged by size: 2522 2219 617 547 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3712 4094 4729 275 Size: 53 83 186 936 2179 382 635 1451 Fragments arranged by size: 2179 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4338 4736 5010 5157 5568 4338 Size: 398 274 147 411 4675 Fragments arranged by size: 4675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2697 2727 1178 Size: 30 165 30 1294 30 4356 Fragments arranged by size: 4356 1294 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2718 3061 3135 1186 Size: 1532 343 74 3956 Fragments arranged by size: 3956 1532 343 74 BseYI C'CCAG_C Cuts at: 2644 5108 5604 2644 Size: 2464 496 2945 Fragments arranged by size: 2945 2464 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2652 1163 Size: 573 916 4416 Fragments arranged by size: 4416 916 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3966 4051 5122 3966 Size: 85 1071 4749 Fragments arranged by size: 4749 1071 85 BsmI GAATG_Cn' Cuts at: 3181 3416 3509 3181 Size: 235 93 5577 Fragments arranged by size: 5577 235 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2033 2279 3163 3224 3966 4051 Size: 399 225 18 246 884 61 742 85 Cuts at: 4051 5122 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 884 742 399 246 225 85 61 18 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3089 3792 1394 Size: 429 1266 703 3507 Fragments arranged by size: 3507 1266 703 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2658 4400 4574 65 Size: 1183 1410 1742 174 1396 Fragments arranged by size: 1742 1410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4549 4736 1700 Size: 110 2739 187 2869 Fragments arranged by size: 2869 2739 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 2145 4709 2145 Size: 2564 3341 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstXI CCAn_nnnn'nTGG Cuts at: 2189 2189 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2983 3235 3602 3987 4004 5456 5542 5554 Size: 1011 252 367 385 17 1452 86 12 Cuts at: 5554 5900 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 252 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4414 Fragments arranged by size: 4414 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 3421 4215 4235 3421 Size: 794 20 5091 Fragments arranged by size: 5091 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 2107 3209 2107 Size: 1102 4803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2179 3352 4241 Size: 54 1425 50 275 18 349 1173 889 Cuts at: 4241 5870 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1173 889 349 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2234 2241 3051 3835 1353 Size: 881 7 810 784 3423 Fragments arranged by size: 3423 881 810 784 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2721 2817 2900 3152 3968 4565 5352 Size: 393 1089 96 83 252 816 597 787 Cuts at: 5352 5620 1239 Size: 268 1524 Fragments arranged by size: 1524 1089 816 787 597 393 268 252 96 83 EcoNI CCTnn'n_nnAGG Cuts at: 2703 2703 Size: 5905 EcoO109I rG'GnC_Cy Cuts at: 2708 2929 2999 2708 Size: 221 70 5614 Fragments arranged by size: 5614 221 70 EcoRI G'AATT_C Cuts at: 2771 3214 3592 2771 Size: 443 378 5084 Fragments arranged by size: 5084 443 378 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 5052 1678 Size: 3374 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 4546 4578 4620 Size: 32 135 32 722 32 2220 32 42 Cuts at: 4620 4652 1373 Size: 32 2626 Fragments arranged by size: 2626 2220 722 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2580 3249 3495 669 Size: 988 281 642 669 246 3079 Fragments arranged by size: 3079 988 669 642 281 246 HindIII A'AGCT_T Cuts at: 748 2340 748 Size: 1592 4313 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5019 5203 1305 Size: 3714 184 2007 Fragments arranged by size: 3714 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2181 10 Size: 54 1425 343 349 3734 Fragments arranged by size: 3734 1425 349 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 3863 4222 4381 518 Size: 648 1021 1676 359 159 2042 Fragments arranged by size: 2042 1676 1021 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2125 3321 3998 5146 5391 1606 Size: 519 1196 677 1148 245 2120 Fragments arranged by size: 2120 1196 1148 677 519 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4414 Fragments arranged by size: 4414 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2733 3157 3263 4808 1132 Size: 97 1504 424 106 1545 2229 Fragments arranged by size: 2229 1545 1504 424 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PfoI T'CCnGG_A Cuts at: 2047 2047 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2697 2729 3974 4006 5487 5519 2697 Size: 32 1245 32 1481 32 3083 Fragments arranged by size: 3083 1481 1245 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2708 2708 Size: 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2029 2029 Size: 5905 PstI C_TGCA'G Cuts at: 830 2260 3257 830 Size: 1430 997 3478 Fragments arranged by size: 3478 1430 997 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SapI GCTCTTCn'nnn_ Cuts at: 2241 3051 2241 Size: 810 5095 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2225 2256 3253 4388 5069 5260 826 Size: 219 1180 31 997 1135 681 191 1471 Fragments arranged by size: 1471 1180 1135 997 681 219 191 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2325 2979 4009 4910 5172 820 Size: 197 926 382 654 1030 901 262 1553 Fragments arranged by size: 1553 1030 926 901 654 382 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2004 3344 513 Size: 552 939 1340 3074 Fragments arranged by size: 3074 1340 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2636 2961 3105 3932 3949 4102 4287 Size: 1255 1056 325 144 827 17 153 185 Cuts at: 4287 4732 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1056 827 445 325 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2113 2399 4151 Size: 274 80 33 51 1443 136 286 1752 Cuts at: 4151 5863 96 Size: 1712 138 Fragments arranged by size: 1752 1712 1443 286 274 138 136 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 2376 2812 4034 2376 Size: 436 1222 4247 Fragments arranged by size: 4247 1222 436 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI