(Circular) (Six-base) MAPSORT of: pFN21AA1147.seq Check: 1001 from: 1 to: 6070 pFN21AA1147 6070 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3051 3051 Size: 6070 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3003 3880 278 Size: 53 83 186 936 1467 877 2468 Fragments arranged by size: 2468 1467 936 877 186 83 53 AccI GT'mk_AC Cuts at: 1937 2640 3003 3413 1937 Size: 703 363 410 4594 Fragments arranged by size: 4594 703 410 363 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 2850 4197 4570 1563 Size: 1287 1347 373 3063 Fragments arranged by size: 3063 1347 1287 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3077 4133 5517 5785 1632 Size: 1445 1056 1384 268 1917 Fragments arranged by size: 1917 1445 1384 1056 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 2667 4969 5946 1203 Size: 1464 2302 977 1327 Fragments arranged by size: 2302 1464 1327 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2645 1184 Size: 1461 4609 AlwNI CAG_nnn'CTG Cuts at: 3052 3115 3813 5385 3052 Size: 63 698 1572 3737 Fragments arranged by size: 3737 1572 698 63 ApaI G_GGCC'C Cuts at: 2054 2391 2054 Size: 337 5733 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 2756 3379 3605 3757 5866 1493 Size: 1263 623 226 152 2109 1697 Fragments arranged by size: 2109 1697 1263 623 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2159 2195 2269 3264 3395 1870 Size: 73 216 36 74 995 131 4545 Fragments arranged by size: 4545 995 216 131 74 73 36 BamHI G'GATC_C Cuts at: 3400 3767 3400 Size: 367 5703 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2120 2199 2532 2873 Size: 325 217 627 239 94 79 333 341 Cuts at: 2873 3391 4846 618 Size: 518 1455 1842 Fragments arranged by size: 1842 1455 627 518 341 333 325 239 217 94 79 BanII G_rGCy'C Cuts at: 1391 2054 2160 2391 3389 1391 Size: 663 106 231 998 4072 Fragments arranged by size: 4072 998 663 231 106 BbeI G_GCGC'C Cuts at: 2030 2124 2203 2030 Size: 94 79 5897 Fragments arranged by size: 5897 94 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3032 3147 928 Size: 971 1133 115 3851 Fragments arranged by size: 3851 1133 971 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 1548 Size: 6070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2109 2129 2149 Size: 107 122 71 631 605 437 20 20 Cuts at: 2149 4681 136 Size: 2532 1525 Fragments arranged by size: 2532 1525 631 605 437 122 107 71 20 20 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 2054 2391 2876 4131 5287 1790 Size: 264 337 485 1255 1156 2573 Fragments arranged by size: 2573 1255 1156 485 337 264 BmgBI CAC'GTC Cuts at: 1318 2218 1318 Size: 900 5170 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3801 4759 442 Size: 416 53 83 399 24 2384 958 1753 Fragments arranged by size: 2384 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2951 3338 4730 1239 Size: 986 726 387 1392 2579 Fragments arranged by size: 2579 1392 986 726 387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2283 4195 5060 5358 2283 Size: 1912 865 298 2995 Fragments arranged by size: 2995 1912 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3285 4733 882 Size: 617 1786 1448 2219 Fragments arranged by size: 2219 1786 1448 617 BsaAI yAC'GTr Cuts at: 493 1204 2905 3007 3861 493 Size: 711 1701 102 854 2702 Fragments arranged by size: 2702 1701 854 711 102 BsaBI GATnn'nnATC Cuts at: 2684 2744 6064 2684 Size: 60 3320 2690 Fragments arranged by size: 3320 2690 60 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2121 2200 3000 Size: 53 83 186 936 494 94 79 800 Cuts at: 3000 3877 4259 4894 275 Size: 877 382 635 1451 Fragments arranged by size: 1451 936 877 800 635 494 382 186 94 83 79 53 BsaWI w'CCGG_w Cuts at: 3198 3294 4503 4901 5175 5322 5733 3198 Size: 96 1209 398 274 147 411 3535 Fragments arranged by size: 3535 1209 411 398 274 147 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5845 Fragments arranged by size: 5845 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2237 2993 1186 Size: 991 60 756 4263 Fragments arranged by size: 4263 991 756 60 BseYI C'CCAG_C Cuts at: 2040 2097 2209 2695 5273 5769 2040 Size: 57 112 486 2578 496 2341 Fragments arranged by size: 2578 2341 496 486 112 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2941 1163 Size: 573 1205 4292 Fragments arranged by size: 4292 1205 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2129 2133 2172 3520 4281 5309 Size: 275 186 128 4 39 1348 761 1028 Cuts at: 5309 1540 Size: 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 128 39 4 BsiHKAI G_wGCw'C Cuts at: 2601 4131 4216 5287 2601 Size: 1530 85 1071 3384 Fragments arranged by size: 3384 1530 1071 85 BsmI GAATG_Cn' Cuts at: 3581 3674 3581 Size: 93 5977 BsmBI CGTCTCn'nnnn_ Cuts at: 2013 2065 2013 Size: 52 6018 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2160 2391 2601 2876 3389 4131 Size: 399 264 106 231 210 275 513 742 Cuts at: 4131 4216 5287 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 513 399 275 264 231 210 106 85 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3051 3321 3425 844 Size: 2207 270 104 3489 Fragments arranged by size: 3489 2207 270 104 BsrBI CCG'CTC Cuts at: 1394 1823 2268 3957 1394 Size: 429 445 1689 3507 Fragments arranged by size: 3507 1689 445 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4565 4739 65 Size: 1183 3317 174 1396 Fragments arranged by size: 3317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2128 2167 3090 4714 4901 1700 Size: 110 318 39 923 1624 187 2869 Fragments arranged by size: 2869 1624 923 318 187 110 39 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 2448 1925 Size: 523 5547 BssSI C'ACGA_G Cuts at: 4130 5142 4130 Size: 1012 5058 BstAPI GCAn_nnn'nTGC Cuts at: 794 3052 3260 794 Size: 2258 208 3604 Fragments arranged by size: 3604 2258 208 BstBI TT'CG_AA Cuts at: 2770 4874 2770 Size: 2104 3966 BstEII G'GTnAC_C Cuts at: 1184 2251 1184 Size: 1067 5003 BstYI r'GATC_y Cuts at: 1972 2739 3400 3767 4152 4169 5621 5707 5719 Size: 767 661 367 385 17 1452 86 12 Cuts at: 5719 6065 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 767 661 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2133 2424 513 Size: 552 939 129 291 4159 Fragments arranged by size: 4159 939 552 291 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 2489 2855 3586 4380 4400 2489 Size: 366 731 794 20 4159 Fragments arranged by size: 4159 794 731 366 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 3374 3374 Size: 6070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2126 2130 2169 Size: 54 1425 50 275 18 296 4 39 Cuts at: 2169 2238 2337 2844 3517 4406 6035 8 Size: 69 99 507 673 889 1629 43 Fragments arranged by size: 1629 1425 889 673 507 296 275 99 69 54 50 43 39 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2126 2130 2169 3517 1537 Size: 275 314 4 39 1348 4090 Fragments arranged by size: 4090 1348 314 275 39 4 EarI CTCTTCn'nnn_ Cuts at: 1353 3153 3173 4000 1353 Size: 1800 20 827 3423 Fragments arranged by size: 3423 1800 827 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4657 5031 5177 387 Size: 1064 138 3068 374 146 1280 Fragments arranged by size: 3068 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2951 3077 3338 4133 4730 5517 Size: 393 593 726 126 261 795 597 787 Cuts at: 5517 5785 1239 Size: 268 1524 Fragments arranged by size: 1524 795 787 726 597 593 393 268 261 126 EcoNI CCTnn'n_nnAGG Cuts at: 2069 3317 2069 Size: 1248 4822 EcoO109I rG'GnC_Cy Cuts at: 2050 2051 2074 2346 2387 2388 3163 2050 Size: 1 23 272 41 1 775 4957 Fragments arranged by size: 4957 775 272 41 23 1 1 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2407 2407 Size: 6070 FseI GG_CCGG'CC Cuts at: 2132 2132 Size: 6070 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 2030 2124 2203 5217 1678 Size: 352 94 79 3014 2531 Fragments arranged by size: 3014 2531 352 94 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2315 2347 2724 Size: 32 135 32 680 32 31 32 377 Cuts at: 2724 2756 2776 2808 4711 4743 4785 4817 1373 Size: 32 20 32 1903 32 42 32 2626 Fragments arranged by size: 2626 1903 680 377 135 42 32 32 32 32 32 32 32 32 31 20 HincII GTy'rAC Cuts at: 669 1657 1938 3414 3660 669 Size: 988 281 1476 246 3079 Fragments arranged by size: 3079 1476 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KasI G'GCGC_C Cuts at: 2026 2120 2199 2026 Size: 94 79 5897 Fragments arranged by size: 5897 94 79 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2520 3198 5184 5368 1305 Size: 1215 678 1986 184 2007 Fragments arranged by size: 2007 1986 1215 678 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2339 2846 10 Size: 54 1425 343 507 507 3234 Fragments arranged by size: 3234 1425 507 507 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 4028 4387 4546 518 Size: 648 1257 1605 359 159 2042 Fragments arranged by size: 2042 1605 1257 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 2135 2699 3052 3486 4163 5311 5556 Size: 426 103 564 353 434 677 1148 245 Cuts at: 5556 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 564 434 426 353 245 103 NaeI GCC'GGC Cuts at: 2130 2169 3092 2130 Size: 39 923 5108 Fragments arranged by size: 5108 923 39 NarI GG'CG_CC Cuts at: 2027 2121 2200 2027 Size: 94 79 5897 Fragments arranged by size: 5897 94 79 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4579 Fragments arranged by size: 4579 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NgoMIV G'CCGG_C Cuts at: 2128 2167 3090 2128 Size: 39 923 5108 Fragments arranged by size: 5108 923 39 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 2422 2509 2671 2710 3263 3428 4973 Size: 97 1193 87 162 39 553 165 1545 Cuts at: 4973 1132 Size: 2229 Fragments arranged by size: 2229 1545 1193 553 165 162 97 87 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 2667 4969 2667 Size: 2302 3768 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 1108 Size: 1128 4942 PfoI T'CCnGG_A Cuts at: 3306 3306 Size: 6070 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2727 2759 4139 4171 5652 5684 2727 Size: 32 1380 32 1481 32 3113 Fragments arranged by size: 3113 1481 1380 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2346 3163 2346 Size: 817 5253 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 2720 3640 2720 Size: 920 5150 PspOMI G'GGCC_C Cuts at: 2050 2387 2050 Size: 337 5733 PstI C_TGCA'G Cuts at: 830 2193 3048 3422 830 Size: 1363 855 374 3478 Fragments arranged by size: 3478 1363 855 374 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 PvuII CAG'CTG Cuts at: 2699 2699 Size: 6070 SacII CC_GC'GG Cuts at: 2136 2136 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SanDI GG'GwC_CC Cuts at: 2346 2346 Size: 6070 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SexAI A'CCwGG_T Cuts at: 2229 2523 2229 Size: 294 5776 SfcI C'TryA_G Cuts at: 826 1045 2189 2818 3044 3418 4553 5234 5425 Size: 219 1144 629 226 374 1135 681 191 Cuts at: 5425 826 Size: 1471 Fragments arranged by size: 1471 1144 1135 681 629 374 226 219 191 SfoI GGC'GCC Cuts at: 2028 2122 2201 2028 Size: 94 79 5897 Fragments arranged by size: 5897 94 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 2197 3266 3397 1872 Size: 325 1069 131 4545 Fragments arranged by size: 4545 1069 325 131 SmlI C'TyrA_G Cuts at: 820 1017 1943 2298 4174 5075 5337 820 Size: 197 926 355 1876 901 262 1553 Fragments arranged by size: 1876 1553 926 901 355 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3263 3428 1132 Size: 2131 165 3774 Fragments arranged by size: 3774 2131 165 SrfI GCCC'GGGC Cuts at: 2197 2197 Size: 6070 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 3323 1517 Size: 1806 4264 StyI C'CwwG_G Cuts at: 513 1065 2004 3286 3509 513 Size: 552 939 1282 223 3074 Fragments arranged by size: 3074 1282 939 552 223 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2709 4097 4114 4267 4452 4897 325 Size: 1255 1129 1388 17 153 185 445 1498 Fragments arranged by size: 1498 1388 1255 1129 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2826 3120 4316 Size: 274 80 33 51 1443 849 294 1196 Cuts at: 4316 6028 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1196 849 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6030 6062 6030 Size: 32 6038 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2422 2422 Size: 6070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 2195 3264 3395 1870 Size: 325 1069 131 4545 Fragments arranged by size: 4545 1069 325 131 XmnI GAAnn'nnTTC Cuts at: 2972 4199 2972 Size: 1227 4843 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3001 3878 276 Size: 53 83 186 936 1467 877 2468 Fragments arranged by size: 2468 1467 936 877 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SfiI SwaI