Restriction map for
pFN21AA1145
(Linear) (Six-base) MAPSORT of: KIAA1145.seq Check: 7597 from: 1 to: 1338
KIAA1145.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 406 859 988 1338
Size: 139 267 453 129 350
Fragments arranged by size:
453 350 267 139 129
AfeI AGC'GCT
Cuts at: 0 633 1021 1338
Size: 633 388 317
Fragments arranged by size:
633 388 317
AflII C'TTAA_G
Cuts at: 0 9 1338
Size: 9 1329
AlwNI CAG_nnn'CTG
Cuts at: 0 882 978 1338
Size: 882 96 360
Fragments arranged by size:
882 360 96
ApoI r'AATT_y
Cuts at: 0 128 579 1338
Size: 128 451 759
Fragments arranged by size:
759 451 128
BanI G'GyrC_C
Cuts at: 0 1249 1338
Size: 1249 89
BanII G_rGCy'C
Cuts at: 0 361 1082 1338
Size: 361 721 256
Fragments arranged by size:
721 361 256
BbvCI CC'TCA_GC
Cuts at: 0 821 1338
Size: 821 517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 298 332 547 581 1338
Size: 298 34 215 34 757
Fragments arranged by size:
757 298 215 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 795 1338
Size: 795 543
BglI GCCn_nnn'nGGC
Cuts at: 0 1009 1338
Size: 1009 329
BlpI GC'TnA_GC
Cuts at: 0 812 1338
Size: 812 526
BmgBI CAC'GTC
Cuts at: 0 689 1338
Size: 689 649
BmrI ACTGGGnnnn_n'
Cuts at: 0 1302 1338
Size: 1302 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 934 1096 1338
Size: 799 135 162 242
Fragments arranged by size:
799 242 162 135
Bpu10I CC'TnA_GC
Cuts at: 0 821 1338
Size: 821 517
BsaI GGTCTCn'nnnn_
Cuts at: 0 483 1338
Size: 483 855
BsaAI yAC'GTr
Cuts at: 0 420 1338
Size: 420 918
BsaBI GATnn'nnATC
Cuts at: 0 395 1338
Size: 395 943
BsaWI w'CCGG_w
Cuts at: 0 536 1338
Size: 536 802
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 1006 1338
Size: 604 402 332
Fragments arranged by size:
604 402 332
BseYI C'CCAG_C
Cuts at: 0 287 1338
Size: 287 1051
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1338
Size: 865 473
BsiEI CG_ry'CG
Cuts at: 0 701 1338
Size: 701 637
BsiHKAI G_wGCw'C
Cuts at: 0 361 1082 1338
Size: 361 721 256
Fragments arranged by size:
721 361 256
BsmI GAATG_Cn'
Cuts at: 0 1128 1338
Size: 1128 210
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1218 1338
Size: 1218 120
Bsp1286I G_dGCh'C
Cuts at: 0 361 1082 1338
Size: 361 721 256
Fragments arranged by size:
721 361 256
BspEI T'CCGG_A
Cuts at: 0 536 1338
Size: 536 802
BspHI T'CATG_A
Cuts at: 0 1169 1338
Size: 1169 169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 950 1338
Size: 950 388
BsrDI GCAATG_nn'
Cuts at: 0 441 563 1338
Size: 441 122 775
Fragments arranged by size:
775 441 122
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1104 1338
Size: 1104 234
BstXI CCAn_nnnn'nTGG
Cuts at: 0 724 747 1246 1338
Size: 724 23 499 92
Fragments arranged by size:
724 499 92 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 152 188 267 382 1185 1338
Size: 152 36 79 115 803 153
Fragments arranged by size:
803 153 152 115 79 36
BtsI GCAGTG_nn'
Cuts at: 0 104 669 1338
Size: 104 565 669
Fragments arranged by size:
669 565 104
EaeI y'GGCC_r
Cuts at: 0 698 980 1338
Size: 698 282 358
Fragments arranged by size:
698 358 282
EagI C'GGCC_G
Cuts at: 0 698 1338
Size: 698 640
EarI CTCTTCn'nnn_
Cuts at: 0 1222 1274 1338
Size: 1222 52 64
Fragments arranged by size:
1222 64 52
EcoICRI GAG'CTC
Cuts at: 0 359 1080 1338
Size: 359 721 258
Fragments arranged by size:
721 359 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 406 799 859 934 988 1096 1338
Size: 139 267 393 60 75 54 108 242
Fragments arranged by size:
393 267 242 139 108 75 60 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 886 970 1338
Size: 886 84 368
Fragments arranged by size:
886 368 84
EcoO109I rG'GnC_Cy
Cuts at: 0 617 1338
Size: 617 721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 1338
Size: 264 1074
HaeII r_GCGC'y
Cuts at: 0 557 635 1023 1338
Size: 557 78 388 315
Fragments arranged by size:
557 388 315 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 1215 1247 1338
Size: 151 32 1032 32 91
Fragments arranged by size:
1032 151 91 32 32
MscI TGG'CCA
Cuts at: 0 982 1338
Size: 982 356
MslI CAynn'nnrTG
Cuts at: 0 950 1153 1183 1338
Size: 950 203 30 155
Fragments arranged by size:
950 203 155 30
MspA1I CmG'CkG
Cuts at: 0 291 816 924 1338
Size: 291 525 108 414
Fragments arranged by size:
525 414 291 108
NruI TCG'CGA
Cuts at: 0 1204 1338
Size: 1204 134
NspI r_CATG'y
Cuts at: 0 471 1338
Size: 471 867
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 1338
Size: 447 891
PfoI T'CCnGG_A
Cuts at: 0 68 80 776 1034 1338
Size: 68 12 696 258 304
Fragments arranged by size:
696 304 258 68 12
PmlI CAC'GTG
Cuts at: 0 420 1338
Size: 420 918
PstI C_TGCA'G
Cuts at: 0 111 296 1109 1338
Size: 111 185 813 229
Fragments arranged by size:
813 229 185 111
PvuII CAG'CTG
Cuts at: 0 291 816 924 1338
Size: 291 525 108 414
Fragments arranged by size:
525 414 291 108
SacI G_AGCT'C
Cuts at: 0 361 1082 1338
Size: 361 721 256
Fragments arranged by size:
721 361 256
SfcI C'TryA_G
Cuts at: 0 107 292 1105 1338
Size: 107 185 813 233
Fragments arranged by size:
813 233 185 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1009 1338
Size: 1009 329
SmlI C'TyrA_G
Cuts at: 0 9 1338
Size: 9 1329
SpeI A'CTAG_T
Cuts at: 0 201 1338
Size: 201 1137
SphI G_CATG'C
Cuts at: 0 471 1338
Size: 471 867
StuI AGG'CCT
Cuts at: 0 1012 1338
Size: 1012 326
StyI C'CwwG_G
Cuts at: 0 1043 1338
Size: 1043 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1233 1338
Size: 1233 105
Enzymes that do cut:
AcuI AfeI AflII AlwNI ApoI BanI BanII BbvCI
BcgI BciVI BglI BlpI BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BstAPI BstXI BtgZI BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MscI MslI
MspA1I NruI NspI PflMI PfoI PmlI PstI PvuII
SacI SfcI SfiI SmlI SpeI SphI StuI StyI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BclI BfrBI
BglII Bme1580I BmtI BplI BpuEI BsaHI BsaXI BsiWI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI
EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI PacI PasI
PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI