(Circular) (Six-base) MAPSORT of: pFN21AA1124.seq Check: 2147 from: 1 to: 9838 pFN21AA1124 9838 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6038 6799 6038 Size: 761 9077 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6462 7648 278 Size: 53 83 186 936 4926 1186 2468 Fragments arranged by size: 4926 2468 1186 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7181 1937 Size: 5244 4594 Acc65I G'GTAC_C Cuts at: 7159 7159 Size: 9838 AclI AA'CG_TT Cuts at: 1563 7965 8338 1563 Size: 6402 373 3063 Fragments arranged by size: 6402 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2955 3269 5421 6310 6549 7901 9285 9553 Size: 1323 314 2152 889 239 1352 1384 268 Cuts at: 9553 1632 Size: 1917 Fragments arranged by size: 2152 1917 1384 1352 1323 889 314 268 239 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9641 AflIII A'CryG_T Cuts at: 1203 4620 5196 8737 9714 1203 Size: 3417 576 3541 977 1327 Fragments arranged by size: 3541 3417 1327 977 576 AgeI A'CCGG_T Cuts at: 8669 8669 Size: 9838 AhdI GACnn_n'nnGTC Cuts at: 2797 8567 2797 Size: 5770 4068 AleI CACnn'nnGTG Cuts at: 1166 5330 1166 Size: 4164 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3401 6827 1184 Size: 2217 3426 4195 Fragments arranged by size: 4195 3426 2217 AlwNI CAG_nnn'CTG Cuts at: 2962 3586 4414 4615 4823 5050 5127 6139 6162 Size: 624 828 201 208 227 77 1012 23 Cuts at: 6162 6646 7581 9153 2962 Size: 484 935 1572 3647 Fragments arranged by size: 3647 1572 1012 935 828 624 484 227 208 201 77 23 ApaI G_GGCC'C Cuts at: 2058 3436 6794 6869 2058 Size: 1378 3358 75 5027 Fragments arranged by size: 5027 3358 1378 75 ApaLI G'TGCA_C Cuts at: 2594 5556 5912 7895 9051 2594 Size: 2962 356 1983 1156 3381 Fragments arranged by size: 3381 2962 1983 1156 356 ApoI r'AATT_y Cuts at: 1493 2304 2504 4782 4808 7147 7373 7525 9634 Size: 811 200 2278 26 2339 226 152 2109 Cuts at: 9634 1493 Size: 1697 Fragments arranged by size: 2339 2278 2109 1697 811 226 200 152 26 AseI AT'TA_AT Cuts at: 160 1032 8392 160 Size: 872 7360 1606 Fragments arranged by size: 7360 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9838 AvaI C'yCGr_G Cuts at: 1870 1943 2171 3429 3524 4497 4541 5240 5808 Size: 73 228 1258 95 973 44 699 568 Cuts at: 5808 6592 7163 1870 Size: 784 571 4545 Fragments arranged by size: 4545 1258 973 784 699 571 568 228 95 73 44 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2913 2946 2913 Size: 33 9805 BamHI G'GATC_C Cuts at: 7168 7535 7168 Size: 367 9471 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4025 5213 6026 6533 7159 Size: 325 217 627 2238 1188 813 507 626 Cuts at: 7159 8614 618 Size: 1455 1842 Fragments arranged by size: 2238 1842 1455 1188 813 627 626 507 325 217 BanII G_rGCy'C Cuts at: 1391 2058 3436 3765 4542 4695 4830 5477 5790 Size: 667 1378 329 777 153 135 647 313 Cuts at: 5790 6300 6384 6794 6869 7076 7157 1391 Size: 510 84 410 75 207 81 4072 Fragments arranged by size: 4072 1378 777 667 647 510 410 329 313 207 153 135 84 81 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 2517 6667 928 Size: 971 333 285 4150 4099 Fragments arranged by size: 4150 4099 971 333 285 BbvCI CC'TCA_GC Cuts at: 3437 3918 5666 3437 Size: 481 1748 7609 Fragments arranged by size: 7609 1748 481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2201 2235 4475 4509 8029 8063 1903 Size: 34 264 34 2240 34 3520 34 3678 Fragments arranged by size: 3678 3520 2240 264 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7730 8940 7730 Size: 1210 8628 BclI T'GATC_A Cuts at: 1548 1548 Size: 9838 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4655 8449 Size: 107 122 71 631 605 337 2646 3794 Cuts at: 8449 136 Size: 1525 Fragments arranged by size: 3794 2646 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 9833 9833 Size: 9838 BlpI GC'TnA_GC Cuts at: 2498 3374 3897 4912 6194 7255 2498 Size: 876 523 1015 1282 1061 5081 Fragments arranged by size: 5081 1282 1061 1015 876 523 Bme1580I G_kGCm'C Cuts at: 1790 2058 2598 2720 3436 3772 5218 5231 5560 Size: 268 540 122 716 336 1446 13 329 Cuts at: 5560 5916 6370 6794 6843 6869 7899 9055 1790 Size: 356 454 424 49 26 1030 1156 2573 Fragments arranged by size: 2573 1446 1156 1030 716 540 454 424 356 336 329 268 122 49 26 13 BmgBI CAC'GTC Cuts at: 1318 2101 2632 3118 3130 4115 5272 6430 1318 Size: 783 531 486 12 985 1157 1158 4726 Fragments arranged by size: 4726 1158 1157 985 783 531 486 12 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4453 4833 7569 Size: 416 53 83 399 24 3036 380 2736 Cuts at: 7569 8527 442 Size: 958 1753 Fragments arranged by size: 3036 2736 1753 958 416 399 380 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9838 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6620 6620 Size: 9838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 3326 3389 3617 4007 4094 4415 4655 Size: 815 1272 63 228 390 87 321 240 Cuts at: 4655 4673 5089 5624 7136 8498 1239 Size: 18 416 535 1512 1362 2579 Fragments arranged by size: 2579 1512 1362 1272 815 535 416 390 321 240 228 87 63 18 Bpu10I CC'TnA_GC Cuts at: 2078 3437 3878 3918 4694 5666 6149 2078 Size: 1359 441 40 776 972 483 5767 Fragments arranged by size: 5767 1359 972 776 483 441 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2009 3557 3980 5996 7963 8828 9126 2009 Size: 1548 423 2016 1967 865 298 2721 Fragments arranged by size: 2721 2016 1967 1548 865 423 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2348 2805 2861 6720 8501 882 Size: 617 849 457 56 3859 1781 2219 Fragments arranged by size: 3859 2219 1781 849 617 457 56 BsaAI yAC'GTr Cuts at: 493 1204 2158 2593 5197 6346 6778 7629 493 Size: 711 954 435 2604 1149 432 851 2702 Fragments arranged by size: 2702 2604 1149 954 851 711 435 432 BsaBI GATnn'nnATC Cuts at: 3702 5882 9832 3702 Size: 2180 3950 3708 Fragments arranged by size: 3950 3708 2180 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3843 4710 6459 7645 Size: 53 83 186 936 2310 867 1749 1186 Cuts at: 7645 8027 8662 275 Size: 382 635 1451 Fragments arranged by size: 2310 1749 1451 1186 936 867 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2521 5971 8271 8669 8943 9090 9501 2521 Size: 3450 2300 398 274 147 411 2858 Fragments arranged by size: 3450 2858 2300 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4546 4576 4628 4658 4921 Size: 30 165 30 3143 30 52 30 263 Cuts at: 4921 4951 5117 5147 6371 6401 1178 Size: 30 166 30 1224 30 4615 Fragments arranged by size: 4615 3143 1224 263 166 165 52 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 3352 3647 3824 3827 4274 4567 4703 Size: 1201 965 295 177 3 447 293 136 Cuts at: 4703 4869 5879 6323 6536 1186 Size: 166 1010 444 213 4488 Fragments arranged by size: 4488 1201 1010 965 447 444 295 293 213 177 166 136 3 BseYI C'CCAG_C Cuts at: 2986 5415 7058 9041 9537 2986 Size: 2429 1643 1983 496 3287 Fragments arranged by size: 3287 2429 1983 1643 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2146 2405 2717 3314 3428 3623 5113 Size: 573 410 259 312 597 114 195 1490 Cuts at: 5113 5140 5294 6152 6509 1163 Size: 27 154 858 357 4492 Fragments arranged by size: 4492 1490 858 597 573 410 357 312 259 195 154 114 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7288 8049 9077 1540 Size: 275 186 5287 761 1028 2301 Fragments arranged by size: 5287 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2598 3312 3765 3951 4041 4542 4695 5477 Size: 492 714 453 186 90 501 153 782 Cuts at: 5477 5560 5916 6150 6300 6384 6816 7899 7984 Size: 83 356 234 150 84 432 1083 85 Cuts at: 7984 9055 2106 Size: 1071 2889 Fragments arranged by size: 2889 1083 1071 782 714 501 492 453 432 356 234 186 153 150 90 85 84 83 BsmI GAATG_Cn' Cuts at: 7349 7442 7349 Size: 93 9745 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2106 2598 2720 3312 3436 3765 Size: 399 268 48 492 122 592 124 329 Cuts at: 3765 3772 3951 4041 4542 4695 4830 5218 5231 Size: 7 179 90 501 153 135 388 13 Cuts at: 5231 5477 5560 5790 5916 6150 6300 6370 6384 Size: 246 83 230 126 234 150 70 14 Cuts at: 6384 6794 6816 6843 6869 7076 7157 7899 7984 Size: 410 22 27 26 207 81 742 85 Cuts at: 7984 9055 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 592 501 492 410 399 388 329 268 246 234 230 207 179 153 150 135 126 124 122 90 85 83 81 70 48 27 26 22 14 13 7 BspEI T'CCGG_A Cuts at: 2521 5971 9501 2521 Size: 3450 3530 2858 Fragments arranged by size: 3530 3450 2858 BspHI T'CATG_A Cuts at: 2883 7727 9457 2883 Size: 4844 1730 3264 Fragments arranged by size: 4844 3264 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6038 6324 6799 6831 7193 844 Size: 5194 286 475 32 362 3489 Fragments arranged by size: 5194 3489 475 362 286 32 BsrBI CCG'CTC Cuts at: 1394 1823 3667 4022 5945 6599 6872 7725 1394 Size: 429 1844 355 1923 654 273 853 3507 Fragments arranged by size: 3507 1923 1844 853 654 429 355 273 BsrDI GCAATG_nn' Cuts at: 65 1248 2319 5427 8333 8507 65 Size: 1183 1071 3108 2906 174 1396 Fragments arranged by size: 3108 2906 1396 1183 1071 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2982 5661 7123 8482 8669 1700 Size: 110 1172 2679 1462 1359 187 2869 Fragments arranged by size: 2869 2679 1462 1359 1172 187 110 BsrGI T'GTAC_A Cuts at: 96 2115 6438 96 Size: 2019 4323 3496 Fragments arranged by size: 4323 3496 2019 BssHII G'CGCG_C Cuts at: 1925 5350 6372 1925 Size: 3425 1022 5391 Fragments arranged by size: 5391 3425 1022 BssSI C'ACGA_G Cuts at: 2861 6922 7898 8910 2861 Size: 4061 976 1012 3789 Fragments arranged by size: 4061 3789 1012 976 BstAPI GCAn_nnn'nTGC Cuts at: 794 3316 3586 3859 5127 6646 794 Size: 2522 270 273 1268 1519 3986 Fragments arranged by size: 3986 2522 1519 1268 273 270 BstBI TT'CG_AA Cuts at: 8642 8642 Size: 9838 BstEII G'GTnAC_C Cuts at: 1184 4502 5803 1184 Size: 3318 1301 5219 Fragments arranged by size: 5219 3318 1301 BstXI CCAn_nnnn'nTGG Cuts at: 6883 6883 Size: 9838 BstYI r'GATC_y Cuts at: 1972 2742 4411 4662 5446 6072 6740 7168 7535 Size: 770 1669 251 784 626 668 428 367 Cuts at: 7535 7920 7937 9389 9475 9487 9833 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1669 1452 784 770 668 626 428 385 367 346 251 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2706 2712 3418 5325 5924 6000 6477 Size: 552 1641 6 706 1907 599 76 477 Cuts at: 6477 513 Size: 3874 Fragments arranged by size: 3874 1907 1641 706 599 552 477 76 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2394 2768 4319 487 Size: 683 56 1168 374 1551 6006 Fragments arranged by size: 6006 1551 1168 683 374 56 BtsI GCAGTG_nn' Cuts at: 4872 5125 6140 7354 8148 8168 4872 Size: 253 1015 1214 794 20 6542 Fragments arranged by size: 6542 1214 1015 794 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9803 DraI TTT'AAA Cuts at: 7142 7142 Size: 9838 DraIII CAC_nnn'GTG Cuts at: 2124 3613 5911 2124 Size: 1489 2298 6051 Fragments arranged by size: 6051 2298 1489 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2105 3409 8845 809 Size: 507 789 1304 5436 1802 Fragments arranged by size: 5436 1802 1304 789 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2160 2562 Size: 54 1425 50 275 18 180 150 402 Cuts at: 2562 2709 3825 5896 5997 6003 6042 7285 8174 Size: 147 1116 2071 101 6 39 1243 889 Cuts at: 8174 9803 8 Size: 1629 43 Fragments arranged by size: 2071 1629 1425 1243 1116 889 402 275 180 150 147 101 54 50 43 39 18 6 EagI C'GGCC_G Cuts at: 1537 1812 7285 1537 Size: 275 5473 4090 Fragments arranged by size: 5473 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2248 2889 3245 4092 4530 5387 6687 7768 Size: 895 641 356 847 438 857 1300 1081 Cuts at: 7768 1353 Size: 3423 Fragments arranged by size: 3423 1300 1081 895 857 847 641 438 356 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2636 4673 8425 8799 8945 387 Size: 1064 138 1047 2037 3752 374 146 1280 Fragments arranged by size: 3752 2037 1280 1064 1047 374 146 138 EcoICRI GAG'CTC Cuts at: 3763 4540 4693 5475 6298 6382 3763 Size: 777 153 782 823 84 7219 Fragments arranged by size: 7219 823 782 777 153 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2955 3269 3326 3389 3617 4007 Size: 393 422 901 314 57 63 228 390 Cuts at: 4007 4094 4415 4655 4673 5089 5421 5624 6310 Size: 87 321 240 18 416 332 203 686 Cuts at: 6310 6549 7136 7901 8498 9285 9553 1239 Size: 239 587 765 597 787 268 1524 Fragments arranged by size: 1524 901 787 765 686 597 587 422 416 393 390 332 321 314 268 240 239 228 203 87 63 57 18 EcoO109I rG'GnC_Cy Cuts at: 2055 2947 3301 3433 3991 5047 5314 6516 6682 Size: 892 354 132 558 1056 267 1202 166 Cuts at: 6682 6866 2055 Size: 184 5027 Fragments arranged by size: 5027 1202 1056 892 558 354 267 184 166 132 EcoRI G'AATT_C Cuts at: 7147 7525 7147 Size: 378 9460 EcoRV GAT'ATC Cuts at: 6753 6753 Size: 9838 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2277 2534 3917 4102 2277 Size: 257 1383 185 8013 Fragments arranged by size: 8013 1383 257 185 FspI TGC'GCA Cuts at: 6473 6650 8344 6473 Size: 177 1694 7967 Fragments arranged by size: 7967 1694 177 FspAI rTGC'GCAy Cuts at: 6473 6473 Size: 9838 HaeII r_GCGC'y Cuts at: 1678 2076 3554 8985 1678 Size: 398 1478 5431 2531 Fragments arranged by size: 5431 2531 1478 398 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 2717 2749 2783 Size: 32 135 32 1076 32 37 32 34 Cuts at: 2783 2815 3227 3259 4546 4578 4628 4660 5530 Size: 32 412 32 1287 32 50 32 870 Cuts at: 5530 5562 5802 5834 6103 6135 6949 6981 7004 Size: 32 240 32 269 32 814 32 23 Cuts at: 7004 7036 8479 8511 8553 8585 1373 Size: 32 1443 32 42 32 2626 Fragments arranged by size: 2626 1443 1287 1076 870 814 412 269 240 135 50 42 37 34 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 7182 7428 669 Size: 988 281 5244 246 3079 Fragments arranged by size: 5244 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2516 3327 3938 5966 748 Size: 1768 811 611 2028 4620 Fragments arranged by size: 4620 2028 1768 811 611 HpaI GTT'AAC Cuts at: 7428 7428 Size: 9838 KpnI G_GTAC'C Cuts at: 7163 7163 Size: 9838 MfeI C'AATT_G Cuts at: 7437 7437 Size: 9838 MluI A'CGCG_T Cuts at: 9714 9714 Size: 9838 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3132 3291 6648 6780 7077 8952 9136 1305 Size: 1827 159 3357 132 297 1875 184 2007 Fragments arranged by size: 3357 2007 1875 1827 297 184 159 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 3827 5999 10 Size: 54 1425 343 732 1263 2172 3849 Fragments arranged by size: 3849 2172 1425 1263 732 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5330 6809 7796 8155 8314 518 Size: 648 4164 1479 987 359 159 2042 Fragments arranged by size: 4164 2042 1479 987 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2197 3298 3583 3796 4183 4393 4588 Size: 435 156 1101 285 213 387 210 195 Cuts at: 4588 5024 5741 5831 7062 7254 7931 9079 9324 Size: 436 717 90 1231 192 677 1148 245 Cuts at: 9324 1606 Size: 2120 Fragments arranged by size: 2120 1231 1148 1101 717 677 436 435 387 285 245 213 210 195 192 156 90 NaeI GCC'GGC Cuts at: 2984 7125 2984 Size: 4141 5697 NcoI C'CATG_G Cuts at: 513 1065 6477 513 Size: 552 5412 3874 Fragments arranged by size: 5412 3874 552 NdeI CA'TA_TG Cuts at: 387 5356 387 Size: 4969 4869 NgoMIV G'CCGG_C Cuts at: 2982 7123 2982 Size: 4141 5697 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9838 NotI GC'GGCC_GC Cuts at: 7285 7285 Size: 9838 NspI r_CATG'y Cuts at: 1132 1229 2818 3023 4624 5687 6808 7196 8741 Size: 97 1589 205 1601 1063 1121 388 1545 Cuts at: 8741 1132 Size: 2229 Fragments arranged by size: 2229 1601 1589 1545 1121 1063 388 205 97 PasI CC'CwG_GG Cuts at: 1348 5868 1348 Size: 4520 5318 PciI A'CATG_T Cuts at: 4620 8737 4620 Size: 4117 5721 PflMI CCAn_nnn'nTGG Cuts at: 1108 3200 4183 4615 5443 5995 6483 1108 Size: 2092 983 432 828 552 488 4463 Fragments arranged by size: 4463 2092 983 828 552 488 432 PfoI T'CCnGG_A Cuts at: 2046 4683 6902 2046 Size: 2637 2219 4982 Fragments arranged by size: 4982 2637 2219 PmeI GTTT'AAAC Cuts at: 7142 7142 Size: 9838 PmlI CAC'GTG Cuts at: 5197 6778 5197 Size: 1581 8257 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7907 7939 9420 9452 7907 Size: 32 1481 32 8293 Fragments arranged by size: 8293 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2947 3301 5314 6682 2947 Size: 354 2013 1368 6103 Fragments arranged by size: 6103 2013 1368 354 PshAI GACnn'nnGTC Cuts at: 1613 5641 1613 Size: 4028 5810 PsiI TTA'TAA Cuts at: 7408 7408 Size: 9838 PspOMI G'GGCC_C Cuts at: 2054 3432 6790 6865 2054 Size: 1378 3358 75 5027 Fragments arranged by size: 5027 3358 1378 75 PspXI vC'TCGA_Gb Cuts at: 2171 3524 4541 2171 Size: 1353 1017 7468 Fragments arranged by size: 7468 1353 1017 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6899 6931 6899 Size: 32 9806 PstI C_TGCA'G Cuts at: 830 2751 2972 3321 3729 4194 4410 4644 6705 Size: 1921 221 349 408 465 216 234 2061 Cuts at: 6705 7190 830 Size: 485 3478 Fragments arranged by size: 3478 2061 1921 485 465 408 349 234 221 216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9838 PvuII CAG'CTG Cuts at: 2041 2197 3796 4183 4393 5741 2041 Size: 156 1599 387 210 1348 6138 Fragments arranged by size: 6138 1599 1348 387 210 156 SacI G_AGCT'C Cuts at: 3765 4542 4695 5477 6300 6384 3765 Size: 777 153 782 823 84 7219 Fragments arranged by size: 7219 823 782 777 153 84 SalI G'TCGA_C Cuts at: 1936 7180 1936 Size: 5244 4594 SapI GCTCTTCn'nnn_ Cuts at: 2889 3245 4530 5387 2889 Size: 356 1285 857 7340 Fragments arranged by size: 7340 1285 857 356 SbfI CC_TGCA'GG Cuts at: 2751 3729 7190 2751 Size: 978 3461 5399 Fragments arranged by size: 5399 3461 978 ScaI AGT'ACT Cuts at: 8086 9798 8086 Size: 1712 8126 SexAI A'CCwGG_T Cuts at: 6225 6225 Size: 9838 SfcI C'TryA_G Cuts at: 826 1045 2747 2968 3317 3725 4190 4406 4640 Size: 219 1702 221 349 408 465 216 234 Cuts at: 4640 6409 6701 7186 8321 9002 9193 826 Size: 1769 292 485 1135 681 191 1471 Fragments arranged by size: 1769 1702 1471 1135 681 485 465 408 349 292 234 221 219 216 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9838 SmaI CCC'GGG Cuts at: 1872 4499 7165 1872 Size: 2627 2666 4545 Fragments arranged by size: 4545 2666 2627 SmlI C'TyrA_G Cuts at: 820 1017 1943 2024 2171 3524 3536 3995 4541 Size: 197 926 81 147 1353 12 459 546 Cuts at: 4541 6011 7942 8843 9105 820 Size: 1470 1931 901 262 1553 Fragments arranged by size: 1931 1553 1470 1353 926 901 546 459 262 197 147 81 12 SnaBI TAC'GTA Cuts at: 493 7629 493 Size: 7136 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9838 SphI G_CATG'C Cuts at: 1132 3023 6808 7196 1132 Size: 1891 3785 388 3774 Fragments arranged by size: 3785 3774 1891 388 SspI AAT'ATT Cuts at: 5 52 3150 7762 5 Size: 47 3098 4612 2081 Fragments arranged by size: 4612 3098 2081 47 StuI AGG'CCT Cuts at: 1517 3923 4121 5257 7113 1517 Size: 2406 198 1136 1856 4242 Fragments arranged by size: 4242 2406 1856 1136 198 StyI C'CwwG_G Cuts at: 513 1065 2013 3684 3927 4722 5691 6477 7277 Size: 552 948 1671 243 795 969 786 800 Cuts at: 7277 513 Size: 3074 Fragments arranged by size: 3074 1671 969 948 800 795 786 552 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2465 2795 3413 4547 5080 5429 6440 Size: 1255 885 330 618 1134 533 349 1011 Cuts at: 6440 6475 7865 7882 8035 8220 8665 325 Size: 35 1390 17 153 185 445 1498 Fragments arranged by size: 1498 1390 1255 1134 1011 885 618 533 445 349 330 185 153 35 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2115 6438 8084 Size: 274 80 33 51 1443 138 4323 1646 Cuts at: 8084 9796 96 Size: 1712 138 Fragments arranged by size: 4323 1712 1646 1443 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4574 4606 5117 5149 6330 6362 6371 6403 9798 Size: 32 511 32 1181 32 9 32 3395 Cuts at: 9798 9830 4574 Size: 32 4582 Fragments arranged by size: 4582 3395 1181 511 32 32 32 32 32 9 Tth111I GACn'n_nGTC Cuts at: 1316 2618 1316 Size: 1302 8536 XbaI T'CTAG_A Cuts at: 5629 7174 5629 Size: 1545 8293 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3115 3835 4720 5047 5257 3115 Size: 720 885 327 210 7696 Fragments arranged by size: 7696 885 720 327 210 XhoI C'TCGA_G Cuts at: 1943 2171 3524 4541 1943 Size: 228 1353 1017 7240 Fragments arranged by size: 7240 1353 1017 228 XmaI C'CCGG_G Cuts at: 1870 4497 7163 1870 Size: 2627 2666 4545 Fragments arranged by size: 4545 2666 2627 XmnI GAAnn'nnTTC Cuts at: 4467 6089 7967 4467 Size: 1622 1878 6338 Fragments arranged by size: 6338 1878 1622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6460 7646 276 Size: 53 83 186 936 4926 1186 2468 Fragments arranged by size: 4926 2468 1186 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI KasI NarI NruI NsiI PacI RsrII SacII SanDI SfiI SfoI SrfI SwaI