Restriction map for
pFN21AA1116
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BfrI BlnI BsaAI BseAI
BsiWI Bsp1407I BspEI BspHI BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 1935 GGCGCC G[GA]^CG[CT]C
AflIII 123 ACATGT A^C[GA][CT]GT
AspHI 1580 GAGCTC G[AT]GC[AT]^C
BalI 440 TGGCCA TGG^CCA
BbeI 1935 GGCGCC GGCGC^C
BclI 1770 TGATCA T^GATCA
BglI 3273 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3667 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3667 GCTCAGC GC^T[ACGT]AGC
BsaBI 1687 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1935 GGCGCC G[GA]^CG[CT]C
BsaI 3245 GAGACC GGTCTC(1/5)
BsgI 2146 GTGCAG GTGCAG(16/14)
BsiEI 1330 CGGTCG CG[GA][CT]^CG
BsiHKAI 1580 GAGCTC G[AT]GC[AT]^C
BsmBI 1828 GAGACG CGTCTC(1/5)
BsmI 1023 GCATTC GAATGC(1/-1)
BspDI 3383 ATCGAT AT^CGAT
BspLU11I 123 ACATGT A^CATGT
BstBI 63 TTCGAA TT^CGAA
CelII 3667 GCTCAGC GC^T[ACGT]AGC
CfoI 1936 GCGC GCG^C
ClaI 3383 ATCGAT AT^CGAT
DraI 1647 TTTAAA TTT^AAA
EcoNI 3464 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1571 ATGCAT ATGCA^T
FauI 1298 CCCGC CCCGC(4/6)
FbaI 1770 TGATCA T^GATCA
HaeII 1935 GGCGCC [GA]GCGC^[CT]
HgaI 1030 GCGTC GACGC(5/10)
HhaI 1936 GCGC GCG^C
Hin1I 1935 GGCGCC G[GA]^CG[CT]C
HinP1I 1936 GCGC G^CGC
HincII 2930 GTCAAC GT[CT]^[GA]AC
HindII 2930 GTCAAC GT[CT]^[GA]AC
KasI 1935 GGCGCC G^GCGCC
MaeII 869 ACGT A^CGT
MamI 1687 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 440 TGGCCA TGG^CCA
MscI 440 TGGCCA TGG^CCA
NarI 1935 GGCGCC GG^CGCC
NcoI 1714 CCATGG C^CATGG
NsiI 1571 ATGCAT ATGCA^T
NspV 63 TTCGAA TT^CGAA
PpuMI 3141 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 1580 GAGCTC GAGCT^C
SexAI 2067 ACCTGGT A^CC[AT]GGT
SfoI 1935 GGCGCC GGC^GCC
SfuI 63 TTCGAA TT^CGAA
SmaI 1532 CCCGGG CCC^GGG
XbaI 1264 TCTAGA T^CTAGA
XmaCI 1532 CCCGGG C^CCGGG
XmaI 1532 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2971 GTAGAC GT^[AC][GT]AC
3083 GTAGAC
AgeI 3047 ACCGGT A^CCGGT
3321 ACCGGT
AocI 988 CCTCAGG CC^T[ACGT]AGG
3634 CCTCAGG
AseI 615 ATTAAT AT^TAAT
1516 ATTAAT
AsnI 615 ATTAAT AT^TAAT
1516 ATTAAT
Asp700I 12 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1202 GAACACATTC
AvaI 1532 CCCGGG C^[CT]CG[GA]G
3578 CTCGGG
BanI 1935 GGCGCC G^G[CT][GA]CC
2799 GGCACC
BanII 1580 GAGCTC G[GA]GC[CT]^C
1848 GAGCCC
BciVI 2999 GGATAC GTATCC(6/5)
2735 GTATCC
BglII 578 AGATCT A^GATCT
1402 AGATCT
BmyI 1580 GAGCTC G[AGT]GC[ACT]^C
1848 GAGCCC
BsoBI 1532 CCCGGG C^[CT]CG[GA]G
3578 CTCGGG
Bsp1286I 1580 GAGCTC G[AGT]GC[ACT]^C
1848 GAGCCC
BsrBI 1299 CCGCTC CCGCTC(-3/-3)
2246 CCGCTC
Bsu36I 988 CCTCAGG CC^T[ACGT]AGG
3634 CCTCAGG
DraII 668 AGGCCCT [GA]G^G[ACGT]CC[CT]
3141 AGGTCCT
Eco81I 988 CCTCAGG CC^T[ACGT]AGG
3634 CCTCAGG
EcoO109I 668 AGGCCCT [GA]G^G[ACGT]CC[CT]
3141 AGGTCCT
MslI 453 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1515 CATTAATATG
NspI 123 ACATGT [GA]CATG^[CT]
3663 ACATGC
PinAI 3047 ACCGGT A^CCGGT
3321 ACCGGT
PshAI 1872 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2773 GACATTCGTC
PshBI 615 ATTAAT AT^TAAT
1516 ATTAAT
SapI 1582 GCTCTTC GCTCTTC(1/4)
2881 GCTCTTC
ScaI 336 AGTACT AGT^ACT
1426 AGTACT
SspI 2539 AATATT AAT^ATT
2559 AATATT
XcmI 2328 CCAGATTTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3265 CCAGAGAAGCCCTGG
XmnI 12 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1202 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BcnI BfaI BpmI BpuAI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraIII DsaI EaeI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII