Restriction map for
pFN21AA1116
(Linear) (Six-base) MAPSORT of: KIAA1116.seq Check: 5652 from: 1 to: 3813
KIAA1116.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AccI GT'mk_AC
Cuts at: 0 2972 3084 3813
Size: 2972 112 729
Fragments arranged by size:
2972 729 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1570 2318 3813
Size: 1094 476 748 1495
Fragments arranged by size:
1495 1094 748 476
AflIII A'CryG_T
Cuts at: 0 123 3813
Size: 123 3690
AgeI A'CCGG_T
Cuts at: 0 3047 3321 3813
Size: 3047 274 492
Fragments arranged by size:
3047 492 274
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3416 3448 3813
Size: 3416 32 365
Fragments arranged by size:
3416 365 32
AlwNI CAG_nnn'CTG
Cuts at: 0 880 1819 2296 3763 3802 3813
Size: 880 939 477 1467 39 11
Fragments arranged by size:
1467 939 880 477 39 11
ApoI r'AATT_y
Cuts at: 0 291 845 2432 2474 2834 3545 3813
Size: 291 554 1587 42 360 711 268
Fragments arranged by size:
1587 711 554 360 291 268 42
AseI AT'TA_AT
Cuts at: 0 616 1517 3813
Size: 616 901 2296
Fragments arranged by size:
2296 901 616
AvaI C'yCGr_G
Cuts at: 0 1532 3578 3813
Size: 1532 2046 235
Fragments arranged by size:
2046 1532 235
BanI G'GyrC_C
Cuts at: 0 1935 2799 3813
Size: 1935 864 1014
Fragments arranged by size:
1935 1014 864
BanII G_rGCy'C
Cuts at: 0 1584 1852 3813
Size: 1584 268 1961
Fragments arranged by size:
1961 1584 268
BbeI G_GCGC'C
Cuts at: 0 1939 3813
Size: 1939 1874
BbsI GAAGACnn'nnnn_
Cuts at: 0 19 818 2967 3189 3465 3813
Size: 19 799 2149 222 276 348
Fragments arranged by size:
2149 799 348 276 222 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 2746 2993 3813
Size: 2746 247 820
Fragments arranged by size:
2746 820 247
BclI T'GATC_A
Cuts at: 0 1770 3813
Size: 1770 2043
BfrBI ATG'CAT
Cuts at: 0 1573 3813
Size: 1573 2240
BglI GCCn_nnn'nGGC
Cuts at: 0 3279 3813
Size: 3279 534
BglII A'GATC_T
Cuts at: 0 578 1402 3813
Size: 578 824 2411
Fragments arranged by size:
2411 824 578
BlpI GC'TnA_GC
Cuts at: 0 3668 3813
Size: 3668 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 2573 3813
Size: 2573 1240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 485 1871 3683 3813
Size: 485 1386 1812 130
Fragments arranged by size:
1812 1386 485 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 455 1841 2069 2897 3813
Size: 404 51 1386 228 828 916
Fragments arranged by size:
1386 916 828 404 228 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1176 1287 3813
Size: 578 598 111 2526
Fragments arranged by size:
2526 598 578 111
BsaI GGTCTCn'nnnn_
Cuts at: 0 3239 3813
Size: 3239 574
BsaBI GATnn'nnATC
Cuts at: 0 1691 3813
Size: 1691 2122
BsaHI Gr'CG_yC
Cuts at: 0 1936 3813
Size: 1936 1877
BsaWI w'CCGG_w
Cuts at: 0 2169 3047 3321 3813
Size: 2169 878 274 492
Fragments arranged by size:
2169 878 492 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 735 765 3813
Size: 735 30 3048
Fragments arranged by size:
3048 735 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 977 1165 1895 1988 3302 3560 3813
Size: 977 188 730 93 1314 258 253
Fragments arranged by size:
1314 977 730 258 253 188 93
BseYI C'CCAG_C
Cuts at: 0 650 3813
Size: 650 3163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2167 3813
Size: 2167 1646
BsiEI CG_ry'CG
Cuts at: 0 1333 3813
Size: 1333 2480
BsiHKAI G_wGCw'C
Cuts at: 0 1584 3813
Size: 1584 2229
BsmI GAATG_Cn'
Cuts at: 0 1023 3813
Size: 1023 2790
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1822 3813
Size: 1822 1991
Bsp1286I G_dGCh'C
Cuts at: 0 1584 1852 3813
Size: 1584 268 1961
Fragments arranged by size:
1961 1584 268
BsrBI CCG'CTC
Cuts at: 0 1301 2248 3813
Size: 1301 947 1565
Fragments arranged by size:
1565 1301 947
BsrFI r'CCGG_y
Cuts at: 0 1989 3047 3321 3813
Size: 1989 1058 274 492
Fragments arranged by size:
1989 1058 492 274
BstBI TT'CG_AA
Cuts at: 0 64 3813
Size: 64 3749
BstYI r'GATC_y
Cuts at: 0 578 1402 1692 3129 3813
Size: 578 824 290 1437 684
Fragments arranged by size:
1437 824 684 578 290
Bsu36I CC'TnA_GG
Cuts at: 0 989 3635 3813
Size: 989 2646 178
Fragments arranged by size:
2646 989 178
BtgI C'CryG_G
Cuts at: 0 529 1714 2898 3813
Size: 529 1185 1184 915
Fragments arranged by size:
1185 1184 915 529
BtsI GCAGTG_nn'
Cuts at: 0 452 3813
Size: 452 3361
ClaI AT'CG_AT
Cuts at: 0 3384 3813
Size: 3384 429
DraI TTT'AAA
Cuts at: 0 1649 3813
Size: 1649 2164
DraIII CAC_nnn'GTG
Cuts at: 0 1417 2581 2899 3813
Size: 1417 1164 318 914
Fragments arranged by size:
1417 1164 914 318
EaeI y'GGCC_r
Cuts at: 0 440 500 2488 3813
Size: 440 60 1988 1325
Fragments arranged by size:
1988 1325 440 60
EarI CTCTTCn'nnn_
Cuts at: 0 1142 1487 1589 2888 3237 3742 3813
Size: 1142 345 102 1299 349 505 71
Fragments arranged by size:
1299 1142 505 349 345 102 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 404 3813
Size: 404 3409
EcoICRI GAG'CTC
Cuts at: 0 1582 3813
Size: 1582 2231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 455 1094 1570 1841 2069 2318 2897
Size: 404 51 639 476 271 228 249 579
Cuts at: 2897 3813
Size: 916
Fragments arranged by size:
916 639 579 476 404 271 249 228
51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3468 3813
Size: 3468 345
EcoO109I rG'GnC_Cy
Cuts at: 0 669 3142 3813
Size: 669 2473 671
Fragments arranged by size:
2473 671 669
EcoRI G'AATT_C
Cuts at: 0 2432 2834 3545 3813
Size: 2432 402 711 268
Fragments arranged by size:
2432 711 402 268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2834 3422 3813
Size: 2834 588 391
Fragments arranged by size:
2834 588 391
HaeII r_GCGC'y
Cuts at: 0 1939 3813
Size: 1939 1874
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 402 434 938 970 3294 3326 3813
Size: 402 32 504 32 2324 32 487
Fragments arranged by size:
2324 504 487 402 32 32 32
HincII GTy'rAC
Cuts at: 0 2932 3813
Size: 2932 881
KasI G'GCGC_C
Cuts at: 0 1935 3813
Size: 1935 1878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2169 2302 3813
Size: 2169 133 1511
Fragments arranged by size:
2169 1511 133
MscI TGG'CCA
Cuts at: 0 442 3813
Size: 442 3371
MslI CAynn'nnrTG
Cuts at: 0 457 1519 3813
Size: 457 1062 2294
Fragments arranged by size:
2294 1062 457
MspA1I CmG'CkG
Cuts at: 0 724 730 736 2257 2707 3813
Size: 724 6 6 1521 450 1106
Fragments arranged by size:
1521 1106 724 450 6 6
NarI GG'CG_CC
Cuts at: 0 1936 3813
Size: 1936 1877
NcoI C'CATG_G
Cuts at: 0 1714 3813
Size: 1714 2099
NsiI A_TGCA'T
Cuts at: 0 1575 3813
Size: 1575 2238
NspI r_CATG'y
Cuts at: 0 127 3667 3813
Size: 127 3540 146
Fragments arranged by size:
3540 146 127
PasI CC'CwG_GG
Cuts at: 0 307 3275 3464 3813
Size: 307 2968 189 349
Fragments arranged by size:
2968 349 307 189
PciI A'CATG_T
Cuts at: 0 123 3813
Size: 123 3690
PflMI CCAn_nnn'nTGG
Cuts at: 0 2068 2707 2899 3163 3813
Size: 2068 639 192 264 650
Fragments arranged by size:
2068 650 639 264 192
PfoI T'CCnGG_A
Cuts at: 0 471 2001 2679 2781 2913 2955 3144 3813
Size: 471 1530 678 102 132 42 189 669
Fragments arranged by size:
1530 678 669 471 189 132 102 42
PpuMI rG'GwC_Cy
Cuts at: 0 3142 3813
Size: 3142 671
PshAI GACnn'nnGTC
Cuts at: 0 1876 2777 3813
Size: 1876 901 1036
Fragments arranged by size:
1876 1036 901
PstI C_TGCA'G
Cuts at: 0 668 729 735 3511 3813
Size: 668 61 6 2776 302
Fragments arranged by size:
2776 668 302 61 6
PvuII CAG'CTG
Cuts at: 0 724 730 736 2257 2707 3813
Size: 724 6 6 1521 450 1106
Fragments arranged by size:
1521 1106 724 450 6 6
SacI G_AGCT'C
Cuts at: 0 1584 3813
Size: 1584 2229
SapI GCTCTTCn'nnn_
Cuts at: 0 1589 2888 3813
Size: 1589 1299 925
Fragments arranged by size:
1589 1299 925
ScaI AGT'ACT
Cuts at: 0 338 1428 3813
Size: 338 1090 2385
Fragments arranged by size:
2385 1090 338
SexAI A'CCwGG_T
Cuts at: 0 2067 3813
Size: 2067 1746
SfcI C'TryA_G
Cuts at: 0 569 664 725 731 1949 3059 3125 3191
Size: 569 95 61 6 1218 1110 66 66
Cuts at: 3191 3507 3813
Size: 316 306
Fragments arranged by size:
1218 1110 569 316 306 95 66 66
61 6
SfoI GGC'GCC
Cuts at: 0 1937 3813
Size: 1937 1876
SmaI CCC'GGG
Cuts at: 0 1534 3813
Size: 1534 2279
SmlI C'TyrA_G
Cuts at: 0 593 1191 1302 3813
Size: 593 598 111 2511
Fragments arranged by size:
2511 598 593 111
SspI AAT'ATT
Cuts at: 0 2541 2561 3813
Size: 2541 20 1252
Fragments arranged by size:
2541 1252 20
StyI C'CwwG_G
Cuts at: 0 513 1622 1714 3406 3813
Size: 513 1109 92 1692 407
Fragments arranged by size:
1692 1109 513 407 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 72 270 2592 3359 3813
Size: 72 198 2322 767 454
Fragments arranged by size:
2322 767 454 198 72
TatI w'GTAC_w
Cuts at: 0 336 1426 2652 3687 3813
Size: 336 1090 1226 1035 126
Fragments arranged by size:
1226 1090 1035 336 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 467 499 735 767 3546 3578 3813
Size: 467 32 236 32 2779 32 235
Fragments arranged by size:
2779 467 236 235 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1264 3813
Size: 1264 2549
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2335 3272 3813
Size: 2335 937 541
Fragments arranged by size:
2335 937 541
XmaI C'CCGG_G
Cuts at: 0 1532 3813
Size: 1532 2281
XmnI GAAnn'nnTTC
Cuts at: 0 16 1206 3813
Size: 16 1190 2607
Fragments arranged by size:
2607 1190 16
Enzymes that do cut:
AccI AcuI AflIII AgeI AloI AlwNI ApoI AseI
AvaI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI BmrI BplI BpmI BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI BstBI BstYI
Bsu36I BtgI BtsI ClaI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NarI
NcoI NsiI NspI PasI PciI PflMI PfoI PpuMI
PshAI PstI PvuII SacI SapI ScaI SexAI SfcI
SfoI SmaI SmlI SspI StyI TaqII TatI TstI
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbvCI BcgI Bme1580I BmgBI BmtI Bpu10I BsaAI BsiWI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstZ17I BtgZI CspCI DrdI EagI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XhoI ZraI