Restriction map for
pFN21AA1101
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BciVI BclI BcnI
BfrI BlnI BpmI BsaAI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I CpoI DraI DraII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HapII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1420 GTCGAC GT^[AC][GT]AC
AcyI 977 GGCGTC G[GA]^CG[CT]C
Asp700I 892 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1108 GAGCTC G[GA]GC[CT]^C
BbvCI 1177 CCTCAGC CCTCAGC(-5/-2)
BcgI 14 CGAGCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1182 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1160 AGATCT A^GATCT
BlpI 1569 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1569 GCTCAGC GC^T[ACGT]AGC
BsaBI 1293 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 977 GGCGTC G[GA]^CG[CT]C
BsaI 1073 GGTCTC GGTCTC(1/5)
BsmFI 41 GGGAC GGGAC(10/14)
BspDI 1487 ATCGAT AT^CGAT
BspHI 272 TCATGA T^CATGA
CelII 1569 GCTCAGC GC^T[ACGT]AGC
CfoI 17 GCGC GCG^C
ClaI 1487 ATCGAT AT^CGAT
DraIII 316 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 603 CCGTGG C^C[GA][CT]GG
EcoRI 1323 GAATTC G^AATTC
HaeII 16 AGCGCC [GA]GCGC^[CT]
HaeIII 1414 GGCC GG^CC
HgaI 978 GCGTC GACGC(5/10)
HhaI 17 GCGC GCG^C
Hin1I 977 GGCGTC G[GA]^CG[CT]C
HinP1I 17 GCGC G^CGC
HincII 1420 GTCGAC GT[CT]^[GA]AC
HindII 1420 GTCGAC GT[CT]^[GA]AC
MaeIII 1495 GTCAC ^GT[ACGT]AC
MamI 1293 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 650 CAATTG C^AATTG
MspA1I 1184 CAGCTG C[AC]G^C[GT]G
MunI 650 CAATTG C^AATTG
PaeR7I 12 CTCGAG C^TCGAG
PvuII 1184 CAGCTG CAG^CTG
RcaI 272 TCATGA T^CATGA
SacI 1108 GAGCTC GAGCT^C
SalI 1420 GTCGAC G^TCGAC
SexAI 963 ACCAGGT A^CC[AT]GGT
SspI 220 AATATT AAT^ATT
Tsp45I 1495 GTCAC ^GT[GC]AC
XhoI 12 CTCGAG C^TCGAG
XmnI 892 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 1232 CCGC CCGC(-3/-1)
670 GCGG
AspHI 920 GAGCAC G[AT]GC[AT]^C
1108 GAGCTC
AvaI 12 CTCGAG C^[CT]CG[GA]G
1079 CCCGAG
AvaII 29 GGTCC G^G[AT]CC
600 GGTCC
BanI 562 GGCACC G^G[CT][GA]CC
579 GGCACC
BbsI 980 GTCTTC GAAGAC(2/6)
1263 GTCTTC
BpuAI 980 GTCTTC GAAGAC(2/6)
1263 GTCTTC
BsiHKAI 920 GAGCAC G[AT]GC[AT]^C
1108 GAGCTC
BsoBI 12 CTCGAG C^[CT]CG[GA]G
1079 CCCGAG
BsrDI 366 CATTGC GCAATG(2/0)
911 CATTGC
Cfr13I 29 GGTCC G^G[ACGT]CC
600 GGTCC
HphI 63 GGTGA GGTGA(8/7)
523 GGTGA
PstI 55 CTGCAG CTGCA^G
718 CTGCAG
Sau96I 29 GGTCC G^G[ACGT]CC
600 GGTCC
ScaI 387 AGTACT AGT^ACT
1308 AGTACT
SfaNI 1510 GCATC GCATC(5/9)
1574 GCATC
TfiI 358 GAATC G^A[AT]TC
1090 GAATC
TspRI 716 CACTG CA[GC]TG[ACGT][ACGT]^
972 CAGTG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
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AcsI AfaI AluI AlwI ApoI
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HinfI
ItaI MaeI MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI SfcI SmlI TaqI
Tru9I TseI Tsp509I TthHB8I XhoII