(Circular) (Six-base) MAPSORT of: pFN21AA1101.seq Check: 6962 from: 1 to: 6286 pFN21AA1101 6286 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3426 3629 1937 Size: 1489 203 4594 Fragments arranged by size: 4594 1489 203 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2610 3255 4349 5733 6001 1632 Size: 978 645 1094 1384 268 1917 Fragments arranged by size: 1917 1384 1094 978 645 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 5185 6162 1203 Size: 3982 977 1327 Fragments arranged by size: 3982 1327 977 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 2346 1166 Size: 1180 5106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6286 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2286 2318 2286 Size: 32 6254 AlwNI CAG_nnn'CTG Cuts at: 4029 5601 4029 Size: 1572 4714 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 2193 2870 2897 3029 3106 3328 3362 3595 Size: 700 677 27 132 77 222 34 233 Cuts at: 3595 3821 3973 6082 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 700 677 233 226 222 152 132 77 34 27 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 2017 3084 3611 1870 Size: 73 74 1067 527 4545 Fragments arranged by size: 4545 1067 527 74 73 BamHI G'GATC_C Cuts at: 3616 3983 3616 Size: 367 5919 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2567 2584 3607 5062 618 Size: 325 217 627 780 17 1023 1455 1842 Fragments arranged by size: 1842 1455 1023 780 627 325 217 17 BanII G_rGCy'C Cuts at: 1391 3117 3605 1391 Size: 1726 488 4072 Fragments arranged by size: 4072 1726 488 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2978 3261 928 Size: 971 1079 283 3953 Fragments arranged by size: 3953 1079 971 283 BbvCI CC'TCA_GC Cuts at: 3183 3183 Size: 6286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2008 2042 4477 4511 1903 Size: 34 71 34 2435 34 3678 Fragments arranged by size: 3678 2435 71 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 1548 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3193 4897 136 Size: 107 122 71 631 605 1521 1704 1525 Fragments arranged by size: 1704 1525 1521 631 605 122 107 71 BglII A'GATC_T Cuts at: 3165 6281 3165 Size: 3116 3170 BlpI GC'TnA_GC Cuts at: 3575 3703 3575 Size: 128 6158 Bme1580I G_kGCm'C Cuts at: 1790 2116 4347 5503 1790 Size: 326 2231 1156 2573 Fragments arranged by size: 2573 2231 1156 326 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4017 4975 442 Size: 416 53 83 399 24 2600 958 1753 Fragments arranged by size: 2600 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3412 3412 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4946 1239 Size: 3707 2579 Bpu10I CC'TnA_GC Cuts at: 3183 3258 3183 Size: 75 6211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 3201 4411 5276 5574 2174 Size: 1027 1210 865 298 2886 Fragments arranged by size: 2886 1210 1027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3084 4949 882 Size: 617 1585 1865 2219 Fragments arranged by size: 2219 1865 1585 617 BsaAI yAC'GTr Cuts at: 493 1204 4077 493 Size: 711 2873 2702 Fragments arranged by size: 2873 2702 711 BsaBI GATnn'nnATC Cuts at: 3302 6280 3302 Size: 2978 3308 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2983 4093 4475 5110 Size: 53 83 186 936 1450 1110 382 635 Cuts at: 5110 275 Size: 1451 Fragments arranged by size: 1451 1450 1110 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2286 2316 1178 Size: 30 165 30 883 30 5148 Fragments arranged by size: 5148 883 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6286 BseYI C'CCAG_C Cuts at: 3009 3191 3570 5489 5985 3009 Size: 182 379 1919 496 3310 Fragments arranged by size: 3310 1919 496 379 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5713 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2929 3117 4347 4432 5503 2929 Size: 188 1230 85 1071 3712 Fragments arranged by size: 3712 1230 1071 188 85 BsmI GAATG_Cn' Cuts at: 3797 3890 3797 Size: 93 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2929 3117 3605 4347 4432 5503 Size: 399 326 813 188 488 742 85 1071 Cuts at: 5503 1391 Size: 2174 Fragments arranged by size: 2174 1071 813 742 488 399 326 188 85 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 2277 4175 5905 2277 Size: 1898 1730 2658 Fragments arranged by size: 2658 1898 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3641 844 Size: 2797 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2370 2915 4781 4955 65 Size: 1183 1122 545 1866 174 1396 Fragments arranged by size: 1866 1396 1183 1122 545 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4930 5117 1700 Size: 110 3120 187 2869 Fragments arranged by size: 3120 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 4346 5358 4346 Size: 1012 5274 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6286 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2430 2827 3165 3297 3616 3983 4368 4385 Size: 458 397 338 132 319 367 385 17 Cuts at: 4385 5837 5923 5935 6281 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 458 397 385 367 346 338 319 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2608 513 Size: 552 1543 4191 Fragments arranged by size: 4191 1543 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5547 Fragments arranged by size: 5547 683 56 BtsI GCAGTG_nn' Cuts at: 2720 3802 4596 4616 2720 Size: 1082 794 20 4390 Fragments arranged by size: 4390 1082 794 20 ClaI AT'CG_AT Cuts at: 1554 3493 1554 Size: 1939 4347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DraIII CAC_nnn'GTG Cuts at: 2326 2326 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251 Size: 54 1425 50 275 18 1903 889 1629 Cuts at: 6251 8 Size: 43 Fragments arranged by size: 1903 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4216 1353 Size: 2863 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3115 3115 Size: 6286 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2610 3255 4349 4946 5733 6001 1239 Size: 393 978 645 1094 597 787 268 1524 Fragments arranged by size: 1524 1094 978 787 645 597 393 268 EcoRI G'AATT_C Cuts at: 3328 3595 3973 3328 Size: 267 378 5641 Fragments arranged by size: 5641 378 267 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2918 2918 Size: 6286 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 2025 5433 1678 Size: 347 3408 2531 Fragments arranged by size: 3408 2531 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3157 3189 4927 4959 5001 Size: 32 135 32 1585 32 1738 32 42 Cuts at: 5001 5033 1373 Size: 32 2626 Fragments arranged by size: 2626 1738 1585 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3427 3630 3876 669 Size: 988 281 1489 203 246 3079 Fragments arranged by size: 3079 1489 988 281 246 203 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 2655 3885 2655 Size: 1230 5056 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2558 3146 5400 5584 1305 Size: 1253 588 2254 184 2007 Fragments arranged by size: 2254 2007 1253 588 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4464 Fragments arranged by size: 4464 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2346 2421 2439 4244 4603 4762 518 Size: 648 1180 75 18 1805 359 159 2042 Fragments arranged by size: 2042 1805 1180 648 359 159 75 18 MspA1I CmG'CkG Cuts at: 1606 3191 3702 4379 5527 5772 1606 Size: 1585 511 677 1148 245 2120 Fragments arranged by size: 2120 1585 1148 677 511 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5734 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 3644 5189 1132 Size: 97 2415 1545 2229 Fragments arranged by size: 2415 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 5185 5185 Size: 6286 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6286 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2286 2318 4355 4387 5868 5900 2286 Size: 32 2037 32 1481 32 2672 Fragments arranged by size: 2672 2037 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PspXI vC'TCGA_Gb Cuts at: 2017 2017 Size: 6286 PstI C_TGCA'G Cuts at: 830 2064 2727 3638 830 Size: 1234 663 911 3478 Fragments arranged by size: 3478 1234 911 663 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 3191 3191 Size: 6286 SacI G_AGCT'C Cuts at: 3117 3117 Size: 6286 SalI G'TCGA_C Cuts at: 1936 3425 3628 1936 Size: 1489 203 4594 Fragments arranged by size: 4594 1489 203 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 2394 3315 4534 6246 2394 Size: 921 1219 1712 2434 Fragments arranged by size: 2434 1712 1219 921 SexAI A'CCwGG_T Cuts at: 2968 2968 Size: 6286 SfcI C'TryA_G Cuts at: 826 1045 2060 2091 2667 2723 3634 4769 5450 Size: 219 1015 31 576 56 911 1135 681 Cuts at: 5450 5641 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1015 911 681 576 219 191 56 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2017 2153 3216 4390 5291 5553 Size: 197 926 74 136 1063 1174 901 262 Cuts at: 5553 820 Size: 1553 Fragments arranged by size: 1553 1174 1063 926 901 262 197 136 74 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3644 1132 Size: 2512 3774 SspI AAT'ATT Cuts at: 5 52 2227 4210 5 Size: 47 2175 1983 2081 Fragments arranged by size: 2175 2081 1983 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 3725 513 Size: 552 2660 3074 Fragments arranged by size: 3074 2660 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3247 4313 4330 4483 4668 5113 325 Size: 1255 1667 1066 17 153 185 445 1498 Fragments arranged by size: 1667 1498 1255 1066 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2392 2482 3313 Size: 274 80 33 51 1443 415 90 831 Cuts at: 3313 4532 6244 96 Size: 1219 1712 138 Fragments arranged by size: 1712 1443 1219 831 415 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6246 6278 6246 Size: 32 6254 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6286 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 2017 1943 Size: 74 6212 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 2901 4415 2901 Size: 1514 4772 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI