Restriction map for
pFN21AA1101
(Linear) (Six-base) MAPSORT of: KIAA1101.seq Check: 3488 from: 1 to: 1581
KIAA1101.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AccI GT'mk_AC
Cuts at: 0 1421 1581
Size: 1421 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1250 1581
Size: 605 645 331
Fragments arranged by size:
645 605 331
AleI CACnn'nnGTG
Cuts at: 0 341 1581
Size: 341 1240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 1581
Size: 281 32 1268
Fragments arranged by size:
1268 281 32
ApoI r'AATT_y
Cuts at: 0 188 865 892 1024 1101 1323 1357 1581
Size: 188 677 27 132 77 222 34 224
Fragments arranged by size:
677 224 222 188 132 77 34 27
AvaI C'yCGr_G
Cuts at: 0 12 1079 1581
Size: 12 1067 502
Fragments arranged by size:
1067 502 12
BanI G'GyrC_C
Cuts at: 0 562 579 1581
Size: 562 17 1002
Fragments arranged by size:
1002 562 17
BanII G_rGCy'C
Cuts at: 0 1112 1581
Size: 1112 469
BbsI GAAGACnn'nnnn_
Cuts at: 0 973 1256 1581
Size: 973 283 325
Fragments arranged by size:
973 325 283
BbvCI CC'TCA_GC
Cuts at: 0 1178 1581
Size: 1178 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3 37 1581
Size: 3 34 1544
Fragments arranged by size:
1544 34 3
BglI GCCn_nnn'nGGC
Cuts at: 0 1188 1581
Size: 1188 393
BglII A'GATC_T
Cuts at: 0 1160 1581
Size: 1160 421
BlpI GC'TnA_GC
Cuts at: 0 1570 1581
Size: 1570 11
Bme1580I G_kGCm'C
Cuts at: 0 111 1581
Size: 111 1470
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1407 1581
Size: 1407 174
Bpu10I CC'TnA_GC
Cuts at: 0 1178 1253 1581
Size: 1178 75 328
Fragments arranged by size:
1178 328 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1196 1581
Size: 169 1027 385
Fragments arranged by size:
1027 385 169
BsaI GGTCTCn'nnnn_
Cuts at: 0 1079 1581
Size: 1079 502
BsaBI GATnn'nnATC
Cuts at: 0 1297 1581
Size: 1297 284
BsaHI Gr'CG_yC
Cuts at: 0 978 1581
Size: 978 603
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 281 311 1581
Size: 281 30 1270
Fragments arranged by size:
1270 281 30
BseYI C'CCAG_C
Cuts at: 0 1004 1186 1565 1581
Size: 1004 182 379 16
Fragments arranged by size:
1004 379 182 16
BsiHKAI G_wGCw'C
Cuts at: 0 924 1112 1581
Size: 924 188 469
Fragments arranged by size:
924 469 188
Bsp1286I G_dGCh'C
Cuts at: 0 111 924 1112 1581
Size: 111 813 188 469
Fragments arranged by size:
813 469 188 111
BspHI T'CATG_A
Cuts at: 0 272 1581
Size: 272 1309
BsrDI GCAATG_nn'
Cuts at: 0 365 910 1581
Size: 365 545 671
Fragments arranged by size:
671 545 365
BstYI r'GATC_y
Cuts at: 0 425 822 1160 1292 1581
Size: 425 397 338 132 289
Fragments arranged by size:
425 397 338 289 132
BtgI C'CryG_G
Cuts at: 0 603 1581
Size: 603 978
BtsI GCAGTG_nn'
Cuts at: 0 715 1581
Size: 715 866
ClaI AT'CG_AT
Cuts at: 0 1488 1581
Size: 1488 93
DraIII CAC_nnn'GTG
Cuts at: 0 321 1581
Size: 321 1260
EcoICRI GAG'CTC
Cuts at: 0 1110 1581
Size: 1110 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1250 1581
Size: 605 645 331
Fragments arranged by size:
645 605 331
EcoRI G'AATT_C
Cuts at: 0 1323 1581
Size: 1323 258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 913 1581
Size: 913 668
HaeII r_GCGC'y
Cuts at: 0 20 1581
Size: 20 1561
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1152 1184 1581
Size: 1152 32 397
Fragments arranged by size:
1152 397 32
HincII GTy'rAC
Cuts at: 0 1422 1581
Size: 1422 159
MfeI C'AATT_G
Cuts at: 0 650 1581
Size: 650 931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1141 1581
Size: 553 588 440
Fragments arranged by size:
588 553 440
MslI CAynn'nnrTG
Cuts at: 0 341 416 434 1581
Size: 341 75 18 1147
Fragments arranged by size:
1147 341 75 18
MspA1I CmG'CkG
Cuts at: 0 1186 1581
Size: 1186 395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 281 313 1581
Size: 281 32 1268
Fragments arranged by size:
1268 281 32
PspXI vC'TCGA_Gb
Cuts at: 0 12 1581
Size: 12 1569
PstI C_TGCA'G
Cuts at: 0 59 722 1581
Size: 59 663 859
Fragments arranged by size:
859 663 59
PvuII CAG'CTG
Cuts at: 0 1186 1581
Size: 1186 395
SacI G_AGCT'C
Cuts at: 0 1112 1581
Size: 1112 469
SalI G'TCGA_C
Cuts at: 0 1420 1581
Size: 1420 161
ScaI AGT'ACT
Cuts at: 0 389 1310 1581
Size: 389 921 271
Fragments arranged by size:
921 389 271
SexAI A'CCwGG_T
Cuts at: 0 963 1581
Size: 963 618
SfcI C'TryA_G
Cuts at: 0 55 86 662 718 1581
Size: 55 31 576 56 863
Fragments arranged by size:
863 576 56 55 31
SmlI C'TyrA_G
Cuts at: 0 12 148 1211 1581
Size: 12 136 1063 370
Fragments arranged by size:
1063 370 136 12
SspI AAT'ATT
Cuts at: 0 222 1581
Size: 222 1359
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1242 1581
Size: 1242 339
TatI w'GTAC_w
Cuts at: 0 387 477 1308 1581
Size: 387 90 831 273
Fragments arranged by size:
831 387 273 90
XhoI C'TCGA_G
Cuts at: 0 12 1581
Size: 12 1569
XmnI GAAnn'nnTTC
Cuts at: 0 896 1581
Size: 896 685
Enzymes that do cut:
AccI AcuI AleI AloI ApoI AvaI BanI BanII
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseYI BsiHKAI
Bsp1286I BspHI BsrDI BstYI BtgI BtsI ClaI DraIII
EcoICRI Eco57MI EcoRI FalI HaeII Hin4I HincII MfeI
MmeI MslI MspA1I PpiI PspXI PstI PvuII SacI
SalI ScaI SexAI SfcI SmlI SspI TaqII TatI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BfrBI BmgBI
BmrI BmtI BpmI BsaAI BsaWI BseRI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI CspCI DraI DrdI EaeI EagI EarI EciI
EcoNI EcoO109I EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI StyI SwaI TstI Tth111I XbaI XcmI
XmaI ZraI