(Circular) (Six-base) MAPSORT of: pFN21AA1081.seq Check: 9652 from: 1 to: 7681 pFN21AA1081 7681 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2296 5491 278 Size: 53 83 186 936 760 3195 2468 Fragments arranged by size: 3195 2468 936 760 186 83 53 AccI GT'mk_AC Cuts at: 1937 5024 1937 Size: 3087 4594 Acc65I G'GTAC_C Cuts at: 5002 5002 Size: 7681 AclI AA'CG_TT Cuts at: 1563 5808 6181 1563 Size: 4245 373 3063 Fragments arranged by size: 4245 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2498 2606 3588 3632 4340 4835 5744 7128 Size: 866 108 982 44 708 495 909 1384 Cuts at: 7128 7396 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 982 909 866 708 495 268 108 44 AflII C'TTAA_G Cuts at: 820 1017 4007 4847 820 Size: 197 2990 840 3654 Fragments arranged by size: 3654 2990 840 197 AflIII A'CryG_T Cuts at: 1203 3612 6580 7557 1203 Size: 2409 2968 977 1327 Fragments arranged by size: 2968 2409 1327 977 AgeI A'CCGG_T Cuts at: 6512 6512 Size: 7681 AhdI GACnn_n'nnGTC Cuts at: 6410 6410 Size: 7681 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7681 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3486 1184 Size: 2302 5379 AlwNI CAG_nnn'CTG Cuts at: 3226 3343 4636 5424 6996 3226 Size: 117 1293 788 1572 3911 Fragments arranged by size: 3911 1572 1293 788 117 ApaLI G'TGCA_C Cuts at: 4353 5738 6894 4353 Size: 1385 1156 5140 Fragments arranged by size: 5140 1385 1156 ApoI r'AATT_y Cuts at: 1493 3174 3216 3305 3552 4990 5216 5368 7477 Size: 1681 42 89 247 1438 226 152 2109 Cuts at: 7477 1493 Size: 1697 Fragments arranged by size: 2109 1697 1681 1438 247 226 152 89 42 AseI AT'TA_AT Cuts at: 160 1032 6235 160 Size: 872 5203 1606 Fragments arranged by size: 5203 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7681 AvaI C'yCGr_G Cuts at: 1870 1943 2916 5006 1870 Size: 73 973 2090 4545 Fragments arranged by size: 4545 2090 973 73 BamHI G'GATC_C Cuts at: 2715 5011 5378 2715 Size: 2296 367 5018 Fragments arranged by size: 5018 2296 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5002 6457 618 Size: 325 217 627 3215 1455 1842 Fragments arranged by size: 3215 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2057 2579 2602 5000 1391 Size: 666 522 23 2398 4072 Fragments arranged by size: 4072 2398 666 522 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2994 4796 928 Size: 971 1095 1802 3813 Fragments arranged by size: 3813 1802 1095 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5872 5906 1903 Size: 34 3935 34 3678 Fragments arranged by size: 3935 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5573 6783 5573 Size: 1210 6471 BclI T'GATC_A Cuts at: 1548 1548 Size: 7681 BfrBI ATG'CAT Cuts at: 3097 3097 Size: 7681 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6292 136 Size: 107 122 71 631 605 4620 1525 Fragments arranged by size: 4620 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3877 7676 3877 Size: 3799 3882 BlpI GC'TnA_GC Cuts at: 2820 5098 2820 Size: 2278 5403 Bme1580I G_kGCm'C Cuts at: 1790 2020 4357 5742 6898 1790 Size: 230 2337 1385 1156 2573 Fragments arranged by size: 2573 2337 1385 1156 230 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7681 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5412 6370 442 Size: 416 53 83 399 24 3995 958 1753 Fragments arranged by size: 3995 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2349 4514 1056 Size: 1293 2165 4223 Fragments arranged by size: 4223 2165 1293 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2593 2684 3609 4724 6341 1239 Size: 1354 91 925 1115 1617 2579 Fragments arranged by size: 2579 1617 1354 1115 925 91 Bpu10I CC'TnA_GC Cuts at: 3456 3456 Size: 7681 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3590 4264 5806 6671 6969 3590 Size: 674 1542 865 298 4302 Fragments arranged by size: 4302 1542 865 674 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3195 6344 882 Size: 617 1696 3149 2219 Fragments arranged by size: 3149 2219 1696 617 BsaAI yAC'GTr Cuts at: 493 1204 5472 493 Size: 711 4268 2702 Fragments arranged by size: 4268 2702 711 BsaBI GATnn'nnATC Cuts at: 2687 7675 2687 Size: 4988 2693 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2293 4749 5488 5870 Size: 53 83 186 936 760 2456 739 382 Cuts at: 5870 6505 275 Size: 635 1451 Fragments arranged by size: 2456 1451 936 760 739 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6114 6512 6786 6933 7344 6114 Size: 398 274 147 411 6451 Fragments arranged by size: 6451 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4760 4790 4928 4958 1178 Size: 30 165 30 3357 30 138 30 3901 Fragments arranged by size: 3901 3357 165 138 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2810 3104 3791 4058 4382 4571 4976 4979 Size: 1624 294 687 267 324 189 405 3 Cuts at: 4979 1186 Size: 3888 Fragments arranged by size: 3888 1624 687 405 324 294 267 189 3 BseYI C'CCAG_C Cuts at: 4626 6884 7380 4626 Size: 2258 496 4927 Fragments arranged by size: 4927 2258 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5131 5892 6920 1540 Size: 275 186 3130 761 1028 2301 Fragments arranged by size: 3130 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4357 5742 5827 6898 4357 Size: 1385 85 1071 5140 Fragments arranged by size: 5140 1385 1071 85 BsmI GAATG_Cn' Cuts at: 3118 5192 5285 3118 Size: 2074 93 5514 Fragments arranged by size: 5514 2074 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2057 2579 2602 4357 5000 5742 Size: 399 230 37 522 23 1755 643 742 Cuts at: 5742 5827 6898 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1755 1071 742 643 522 399 230 85 37 23 BspEI T'CCGG_A Cuts at: 7344 7344 Size: 7681 BspHI T'CATG_A Cuts at: 5570 7300 5570 Size: 1730 5951 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5036 844 Size: 4192 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2022 2830 3631 3799 4498 5568 1394 Size: 429 199 808 801 168 699 1070 3507 Fragments arranged by size: 3507 1070 808 801 699 429 199 168 BsrDI GCAATG_nn' Cuts at: 65 1248 6176 6350 65 Size: 1183 4928 174 1396 Fragments arranged by size: 4928 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6325 6512 1700 Size: 110 4515 187 2869 Fragments arranged by size: 4515 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2656 4480 96 Size: 2560 1824 3297 Fragments arranged by size: 3297 2560 1824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7681 BssSI C'ACGA_G Cuts at: 5741 6753 5741 Size: 1012 6669 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7681 BstBI TT'CG_AA Cuts at: 2857 6485 2857 Size: 3628 4053 BstEII G'GTnAC_C Cuts at: 1184 2096 1184 Size: 912 6769 BstXI CCAn_nnnn'nTGG Cuts at: 2057 2057 Size: 7681 BstYI r'GATC_y Cuts at: 1972 2461 2715 3877 3949 5011 5378 5763 5780 Size: 489 254 1162 72 1062 367 385 17 Cuts at: 5780 7232 7318 7330 7676 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1162 1062 489 385 367 346 254 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 3982 3982 Size: 7681 BtgI C'CryG_G Cuts at: 513 1065 4572 513 Size: 552 3507 3622 Fragments arranged by size: 3622 3507 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6942 Fragments arranged by size: 6942 683 56 BtsI GCAGTG_nn' Cuts at: 2143 2158 2356 3756 5197 5991 6011 2143 Size: 15 198 1400 1441 794 20 3813 Fragments arranged by size: 3813 1441 1400 794 198 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7681 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7646 DraI TTT'AAA Cuts at: 2190 4985 2190 Size: 2795 4886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6688 809 Size: 507 5372 1802 Fragments arranged by size: 5372 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2278 3325 4569 Size: 54 1425 50 275 18 448 1047 1244 Cuts at: 4569 4881 5128 6017 7646 8 Size: 312 247 889 1629 43 Fragments arranged by size: 1629 1425 1244 1047 889 448 312 275 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5128 1537 Size: 275 3316 4090 Fragments arranged by size: 4090 3316 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3204 3510 3564 4343 4446 4728 5611 1353 Size: 1851 306 54 779 103 282 883 3423 Fragments arranged by size: 3423 1851 883 779 306 282 103 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6268 6642 6788 387 Size: 1064 138 4679 374 146 1280 Fragments arranged by size: 4679 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2498 2593 2606 2684 3588 3609 3632 Size: 393 866 95 13 78 904 21 23 Cuts at: 3632 4340 4724 4835 5744 6341 7128 7396 1239 Size: 708 384 111 909 597 787 268 1524 Fragments arranged by size: 1524 909 904 866 787 708 597 393 384 268 111 95 78 23 21 13 EcoNI CCTnn'n_nnAGG Cuts at: 2435 3209 2435 Size: 774 6907 EcoO109I rG'GnC_Cy Cuts at: 2725 3781 2725 Size: 1056 6625 EcoRI G'AATT_C Cuts at: 4990 5368 4990 Size: 378 7303 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3463 3695 3938 4739 3463 Size: 232 243 801 6405 Fragments arranged by size: 6405 801 243 232 FspI TGC'GCA Cuts at: 6187 6187 Size: 7681 HaeII r_GCGC'y Cuts at: 1678 6828 1678 Size: 5150 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3649 3681 4760 4792 4849 Size: 32 135 32 2077 32 1079 32 57 Cuts at: 4849 4881 6322 6354 6396 6428 1373 Size: 32 1441 32 42 32 2626 Fragments arranged by size: 2626 2077 1441 1079 135 57 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5025 5271 669 Size: 988 281 3087 246 3079 Fragments arranged by size: 3087 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2616 2936 748 Size: 1868 320 5493 Fragments arranged by size: 5493 1868 320 HpaI GTT'AAC Cuts at: 5271 5271 Size: 7681 KpnI G_GTAC'C Cuts at: 5006 5006 Size: 7681 MfeI C'AATT_G Cuts at: 3804 4067 5280 3804 Size: 263 1213 6205 Fragments arranged by size: 6205 1213 263 MluI A'CGCG_T Cuts at: 7557 7557 Size: 7681 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2122 3597 4245 6795 6979 1305 Size: 817 1475 648 2550 184 2007 Fragments arranged by size: 2550 2007 1475 817 648 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3327 4571 10 Size: 54 1425 343 1495 1244 3120 Fragments arranged by size: 3120 1495 1425 1244 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2456 2681 3431 5639 5998 6157 Size: 648 1072 218 225 750 2208 359 159 Cuts at: 6157 518 Size: 2042 Fragments arranged by size: 2208 2042 1072 750 648 359 225 218 159 MspA1I CmG'CkG Cuts at: 1606 2476 2737 5097 5774 6922 7167 1606 Size: 870 261 2360 677 1148 245 2120 Fragments arranged by size: 2360 2120 1148 870 677 261 245 NcoI C'CATG_G Cuts at: 513 1065 4572 513 Size: 552 3507 3622 Fragments arranged by size: 3622 3507 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7681 NheI G'CTAG_C Cuts at: 1052 2345 4510 1052 Size: 1293 2165 4223 Fragments arranged by size: 4223 2165 1293 NotI GC'GGCC_GC Cuts at: 5128 5128 Size: 7681 NsiI A_TGCA'T Cuts at: 3099 3099 Size: 7681 NspI r_CATG'y Cuts at: 1132 1229 2680 3616 3965 5039 6584 1132 Size: 97 1451 936 349 1074 1545 2229 Fragments arranged by size: 2229 1545 1451 1074 936 349 97 PasI CC'CwG_GG Cuts at: 1348 2057 1348 Size: 709 6972 PciI A'CATG_T Cuts at: 3612 6580 3612 Size: 2968 4713 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7681 PfoI T'CCnGG_A Cuts at: 2700 2700 Size: 7681 PmeI GTTT'AAAC Cuts at: 4985 4985 Size: 7681 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5750 5782 7263 7295 5750 Size: 32 1481 32 6136 Fragments arranged by size: 6136 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2725 Size: 7681 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7681 PsiI TTA'TAA Cuts at: 5251 5251 Size: 7681 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2098 2130 2098 Size: 32 7649 PstI C_TGCA'G Cuts at: 830 2469 3378 3483 4495 5033 830 Size: 1639 909 105 1012 538 3478 Fragments arranged by size: 3478 1639 1012 909 538 105 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7681 PvuII CAG'CTG Cuts at: 2476 2737 2476 Size: 261 7420 SalI G'TCGA_C Cuts at: 1936 5023 1936 Size: 3087 4594 SapI GCTCTTCn'nnn_ Cuts at: 4446 4728 4446 Size: 282 7399 SbfI CC_TGCA'GG Cuts at: 5033 5033 Size: 7681 ScaI AGT'ACT Cuts at: 3250 5929 7641 3250 Size: 2679 1712 3290 Fragments arranged by size: 3290 2679 1712 SexAI A'CCwGG_T Cuts at: 4154 4154 Size: 7681 SfcI C'TryA_G Cuts at: 826 1045 2465 3374 3479 4415 4491 5029 6164 Size: 219 1420 909 105 936 76 538 1135 Cuts at: 6164 6845 7036 826 Size: 681 191 1471 Fragments arranged by size: 1471 1420 1135 936 909 681 538 219 191 105 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7681 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7681 SmaI CCC'GGG Cuts at: 1872 5008 1872 Size: 3136 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3605 4007 4279 4847 5785 6686 Size: 197 926 1662 402 272 568 938 901 Cuts at: 6686 6948 820 Size: 262 1553 Fragments arranged by size: 1662 1553 938 926 901 568 402 272 262 197 SnaBI TAC'GTA Cuts at: 493 5472 493 Size: 4979 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7681 SphI G_CATG'C Cuts at: 1132 3965 5039 1132 Size: 2833 1074 3774 Fragments arranged by size: 3774 2833 1074 SspI AAT'ATT Cuts at: 5 52 5605 5 Size: 47 5553 2081 Fragments arranged by size: 5553 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7681 StyI C'CwwG_G Cuts at: 513 1065 2973 4572 5120 513 Size: 552 1908 1599 548 3074 Fragments arranged by size: 3074 1908 1599 552 548 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2083 4140 4640 5708 5725 5878 6063 Size: 1255 503 2057 500 1068 17 153 185 Cuts at: 6063 6508 325 Size: 445 1498 Fragments arranged by size: 2057 1498 1255 1068 503 500 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2646 2656 3248 Size: 274 80 33 51 1443 669 10 592 Cuts at: 3248 4480 5927 7639 96 Size: 1232 1447 1712 138 Fragments arranged by size: 1712 1447 1443 1232 669 592 274 138 80 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4895 4927 4928 4960 7641 7673 4895 Size: 32 1 32 2681 32 4903 Fragments arranged by size: 4903 2681 32 32 32 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7681 XbaI T'CTAG_A Cuts at: 5017 5017 Size: 7681 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7681 XmaI C'CCGG_G Cuts at: 1870 5006 1870 Size: 3136 4545 XmnI GAAnn'nnTTC Cuts at: 3666 4722 5810 3666 Size: 1056 1088 5537 Fragments arranged by size: 5537 1088 1056 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2294 5489 276 Size: 53 83 186 936 760 3195 2468 Fragments arranged by size: 3195 2468 936 760 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI