(Circular) (Six-base) MAPSORT of: pFN21AA1080.seq Check: 499 from: 1 to: 6544 pFN21AA1080 6544 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2149 2149 Size: 6544 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4354 278 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3887 1937 Size: 1950 4594 Acc65I G'GTAC_C Cuts at: 3865 3865 Size: 6544 AclI AA'CG_TT Cuts at: 1563 4671 5044 1563 Size: 3108 373 3063 Fragments arranged by size: 3108 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2395 2489 2609 4607 5991 6259 1632 Size: 763 94 120 1998 1384 268 1917 Fragments arranged by size: 1998 1917 1384 763 268 120 94 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6347 AflIII A'CryG_T Cuts at: 1203 5443 6420 1203 Size: 4240 977 1327 Fragments arranged by size: 4240 1327 977 AgeI A'CCGG_T Cuts at: 2637 5375 2637 Size: 2738 3806 AhdI GACnn_n'nnGTC Cuts at: 5273 5273 Size: 6544 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6544 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6544 AlwNI CAG_nnn'CTG Cuts at: 2467 3121 3553 3712 4287 5859 2467 Size: 654 432 159 575 1572 3152 Fragments arranged by size: 3152 1572 654 575 432 159 ApaI G_GGCC'C Cuts at: 2069 2069 Size: 6544 ApaLI G'TGCA_C Cuts at: 4601 5757 4601 Size: 1156 5388 ApoI r'AATT_y Cuts at: 1493 2146 2312 2650 2889 3253 3498 3853 4079 Size: 653 166 338 239 364 245 355 226 Cuts at: 4079 4231 6340 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 653 364 355 338 245 239 226 166 152 AseI AT'TA_AT Cuts at: 160 1032 5098 160 Size: 872 4066 1606 Fragments arranged by size: 4066 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2615 3869 1870 Size: 73 119 553 1254 4545 Fragments arranged by size: 4545 1254 553 119 73 BamHI G'GATC_C Cuts at: 3874 4241 3874 Size: 367 6177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 3534 3865 5320 618 Size: 325 217 627 1310 437 331 1455 1842 Fragments arranged by size: 1842 1455 1310 627 437 331 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2552 3529 3863 1391 Size: 678 483 977 334 4072 Fragments arranged by size: 4072 977 678 483 334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2442 3044 928 Size: 971 543 602 4428 Fragments arranged by size: 4428 971 602 543 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4735 4769 1903 Size: 34 2798 34 3678 Fragments arranged by size: 3678 2798 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4436 5646 4436 Size: 1210 5334 BclI T'GATC_A Cuts at: 1548 2334 1548 Size: 786 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5155 136 Size: 107 122 71 631 605 337 3146 1525 Fragments arranged by size: 3146 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6539 6539 Size: 6544 BlpI GC'TnA_GC Cuts at: 2750 3961 2750 Size: 1211 5333 Bme1580I G_kGCm'C Cuts at: 1790 2069 3293 3537 4605 5761 1790 Size: 279 1224 244 1068 1156 2573 Fragments arranged by size: 2573 1224 1156 1068 279 244 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4275 5233 442 Size: 416 53 83 399 24 2858 958 1753 Fragments arranged by size: 2858 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6544 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3594 3594 Size: 6544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2282 2879 2989 3190 3339 3675 5204 Size: 863 180 597 110 201 149 336 1529 Cuts at: 5204 1239 Size: 2579 Fragments arranged by size: 2579 1529 863 597 336 201 180 149 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3405 4669 5534 5832 3405 Size: 1264 865 298 4117 Fragments arranged by size: 4117 1264 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5207 882 Size: 617 3708 2219 Fragments arranged by size: 3708 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4335 493 Size: 711 3131 2702 Fragments arranged by size: 3131 2702 711 BsaBI GATnn'nnATC Cuts at: 6538 6538 Size: 6544 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4351 4733 5368 275 Size: 53 83 186 936 2818 382 635 1451 Fragments arranged by size: 2818 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2431 2637 2844 3483 4977 5375 5649 5796 6207 Size: 206 207 639 1494 398 274 147 411 Cuts at: 6207 2431 Size: 2768 Fragments arranged by size: 2768 1494 639 411 398 274 207 206 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2672 2702 1178 Size: 30 165 30 1269 30 5020 Fragments arranged by size: 5020 1269 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2900 3193 3257 1186 Size: 1714 293 64 4473 Fragments arranged by size: 4473 1714 293 64 BseYI C'CCAG_C Cuts at: 3452 3582 5747 6243 3452 Size: 130 2165 496 3753 Fragments arranged by size: 3753 2165 496 130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5971 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3994 4755 5783 1540 Size: 275 186 15 1978 761 1028 2301 Fragments arranged by size: 2301 1978 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2552 3575 4605 4690 5761 2552 Size: 1023 1030 85 1071 3335 Fragments arranged by size: 3335 1071 1030 1023 85 BsmI GAATG_Cn' Cuts at: 3671 4055 4148 3671 Size: 384 93 6067 Fragments arranged by size: 6067 384 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2445 2445 Size: 6544 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2552 3293 3529 3537 3575 3863 Size: 399 279 483 741 236 8 38 288 Cuts at: 3863 4605 4690 5761 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 741 483 399 288 279 236 85 38 8 BspEI T'CCGG_A Cuts at: 2431 6207 2431 Size: 3776 2768 BspHI T'CATG_A Cuts at: 4433 6163 4433 Size: 1730 4814 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2149 2791 3050 3085 3899 844 Size: 1305 642 259 35 814 3489 Fragments arranged by size: 3489 1305 814 642 259 35 BsrBI CCG'CTC Cuts at: 1394 1823 4431 1394 Size: 429 2608 3507 Fragments arranged by size: 3507 2608 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5039 5213 65 Size: 1183 3791 174 1396 Fragments arranged by size: 3791 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2637 3646 5188 5375 1700 Size: 110 827 1009 1542 187 2869 Fragments arranged by size: 2869 1542 1009 827 187 110 BsrGI T'GTAC_A Cuts at: 96 2931 96 Size: 2835 3709 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6544 BssSI C'ACGA_G Cuts at: 4604 5616 4604 Size: 1012 5532 BstAPI GCAn_nnn'nTGC Cuts at: 794 2746 794 Size: 1952 4592 BstBI TT'CG_AA Cuts at: 5348 5348 Size: 6544 BstEII G'GTnAC_C Cuts at: 1184 2956 1184 Size: 1772 4772 BstXI CCAn_nnnn'nTGG Cuts at: 2538 2986 2538 Size: 448 6096 BstYI r'GATC_y Cuts at: 1972 2214 3874 4241 4626 4643 6095 6181 6193 Size: 242 1660 367 385 17 1452 86 12 Cuts at: 6193 6539 1972 Size: 346 1977 Fragments arranged by size: 1977 1660 1452 385 367 346 242 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 513 Size: 552 939 12 5041 Fragments arranged by size: 5041 939 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5805 Fragments arranged by size: 5805 683 56 BtsI GCAGTG_nn' Cuts at: 4060 4854 4874 4060 Size: 794 20 5730 Fragments arranged by size: 5730 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6544 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3757 3792 529 Size: 35 3193 35 3281 Fragments arranged by size: 3281 3193 35 35 DraI TTT'AAA Cuts at: 3848 3848 Size: 6544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2999 3827 5551 809 Size: 507 1683 828 1724 1802 Fragments arranged by size: 1802 1724 1683 828 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2307 3991 4880 Size: 54 1425 50 275 18 477 1684 889 Cuts at: 4880 6509 8 Size: 1629 43 Fragments arranged by size: 1684 1629 1425 889 477 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3991 1537 Size: 275 2179 4090 Fragments arranged by size: 4090 2179 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2386 2414 4474 1353 Size: 1033 28 2060 3423 Fragments arranged by size: 3423 2060 1033 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5131 5505 5651 387 Size: 1064 138 3542 374 146 1280 Fragments arranged by size: 3542 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2550 2550 Size: 6544 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2282 2395 2489 2609 2879 2989 Size: 393 470 180 113 94 120 270 110 Cuts at: 2989 3190 3339 3675 4607 5204 5991 6259 1239 Size: 201 149 336 932 597 787 268 1524 Fragments arranged by size: 1524 932 787 597 470 393 336 270 268 201 180 149 120 113 110 94 EcoNI CCTnn'n_nnAGG Cuts at: 3153 3153 Size: 6544 EcoO109I rG'GnC_Cy Cuts at: 2057 2364 2620 2776 2794 3118 2057 Size: 307 256 156 18 324 5483 Fragments arranged by size: 5483 324 307 256 156 18 EcoRI G'AATT_C Cuts at: 3498 3853 4231 3498 Size: 355 378 5811 Fragments arranged by size: 5811 378 355 EcoRV GAT'ATC Cuts at: 3598 3702 3598 Size: 104 6440 FspI TGC'GCA Cuts at: 3081 5050 3081 Size: 1969 4575 HaeII r_GCGC'y Cuts at: 1678 5691 1678 Size: 4013 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2206 2238 2672 2704 3254 Size: 32 135 32 634 32 434 32 550 Cuts at: 3254 3286 5185 5217 5259 5291 1373 Size: 32 1899 32 42 32 2626 Fragments arranged by size: 2626 1899 634 550 434 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3888 4134 669 Size: 988 281 1950 246 3079 Fragments arranged by size: 3079 1950 988 281 246 HindIII A'AGCT_T Cuts at: 748 2582 3662 748 Size: 1834 1080 3630 Fragments arranged by size: 3630 1834 1080 HpaI GTT'AAC Cuts at: 4134 4134 Size: 6544 KpnI G_GTAC'C Cuts at: 3869 3869 Size: 6544 MfeI C'AATT_G Cuts at: 4143 4143 Size: 6544 MluI A'CGCG_T Cuts at: 6420 6420 Size: 6544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 2396 5658 5842 1305 Size: 795 296 3262 184 2007 Fragments arranged by size: 3262 2007 795 296 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2309 10 Size: 54 1425 343 477 4245 Fragments arranged by size: 4245 1425 477 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4502 4861 5020 518 Size: 648 3336 359 159 2042 Fragments arranged by size: 3336 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 3695 3960 4637 5785 6030 1606 Size: 412 1677 265 677 1148 245 2120 Fragments arranged by size: 2120 1677 1148 677 412 265 245 NaeI GCC'GGC Cuts at: 3648 3648 Size: 6544 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5053 Fragments arranged by size: 5053 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6544 NgoMIV G'CCGG_C Cuts at: 3646 3646 Size: 6544 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6544 NotI GC'GGCC_GC Cuts at: 3991 3991 Size: 6544 NspI r_CATG'y Cuts at: 1132 1229 2119 2192 2758 3902 5447 1132 Size: 97 890 73 566 1144 1545 2229 Fragments arranged by size: 2229 1545 1144 890 566 97 73 PasI CC'CwG_GG Cuts at: 1348 2053 3530 1348 Size: 705 1477 4362 Fragments arranged by size: 4362 1477 705 PciI A'CATG_T Cuts at: 5443 5443 Size: 6544 PflMI CCAn_nnn'nTGG Cuts at: 1108 3224 1108 Size: 2116 4428 PmeI GTTT'AAAC Cuts at: 3848 3848 Size: 6544 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4613 4645 6126 6158 4613 Size: 32 1481 32 4999 Fragments arranged by size: 4999 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2364 2620 3118 2057 Size: 307 256 498 5483 Fragments arranged by size: 5483 498 307 256 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6544 PsiI TTA'TAA Cuts at: 2486 4114 2486 Size: 1628 4916 PspOMI G'GGCC_C Cuts at: 2065 2065 Size: 6544 PstI C_TGCA'G Cuts at: 830 2742 2802 3247 3645 3896 830 Size: 1912 60 445 398 251 3478 Fragments arranged by size: 3478 1912 445 398 251 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6544 PvuII CAG'CTG Cuts at: 3695 3695 Size: 6544 SacI G_AGCT'C Cuts at: 2552 2552 Size: 6544 SacII CC_GC'GG Cuts at: 2019 2019 Size: 6544 SalI G'TCGA_C Cuts at: 1936 3886 1936 Size: 1950 4594 SanDI GG'GwC_CC Cuts at: 2057 2057 Size: 6544 SbfI CC_TGCA'GG Cuts at: 2802 3896 2802 Size: 1094 5450 ScaI AGT'ACT Cuts at: 4792 6504 4792 Size: 1712 4832 SexAI A'CCwGG_T Cuts at: 3337 3337 Size: 6544 SfcI C'TryA_G Cuts at: 826 1045 2738 2798 3243 3641 3892 5027 5708 Size: 219 1693 60 445 398 251 1135 681 Cuts at: 5708 5899 826 Size: 191 1471 Fragments arranged by size: 1693 1471 1135 681 445 398 251 219 191 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6544 SmaI CCC'GGG Cuts at: 1872 2064 3871 1872 Size: 192 1807 4545 Fragments arranged by size: 4545 1807 192 SmlI C'TyrA_G Cuts at: 820 1017 1943 3420 4648 5549 5811 820 Size: 197 926 1477 1228 901 262 1553 Fragments arranged by size: 1553 1477 1228 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4335 493 Size: 3842 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6544 SphI G_CATG'C Cuts at: 1132 3902 1132 Size: 2770 3774 SspI AAT'ATT Cuts at: 5 52 4468 5 Size: 47 4416 2081 Fragments arranged by size: 4416 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6544 StyI C'CwwG_G Cuts at: 513 1065 2004 2917 3129 3324 3772 3983 513 Size: 552 939 913 212 195 448 211 3074 Fragments arranged by size: 3074 939 913 552 448 212 211 195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2930 3552 4571 4588 4741 4926 5371 Size: 1255 1350 622 1019 17 153 185 445 Cuts at: 5371 325 Size: 1498 Fragments arranged by size: 1498 1350 1255 1019 622 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2931 4790 6502 Size: 274 80 33 51 1443 954 1859 1712 Cuts at: 6502 96 Size: 138 Fragments arranged by size: 1859 1712 1443 954 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2958 2990 6504 6536 2958 Size: 32 3514 32 2966 Fragments arranged by size: 3514 2966 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6544 XbaI T'CTAG_A Cuts at: 3880 3880 Size: 6544 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2535 3363 2535 Size: 828 5716 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6544 XmaI C'CCGG_G Cuts at: 1870 2062 3869 1870 Size: 192 1807 4545 Fragments arranged by size: 4545 1807 192 XmnI GAAnn'nnTTC Cuts at: 3491 3812 4673 3491 Size: 321 861 5362 Fragments arranged by size: 5362 861 321 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4352 276 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI