(Circular) (Six-base) MAPSORT of: pFN21AA1079.seq Check: 2624 from: 1 to: 9067 pFN21AA1079 9067 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2128 5563 6000 6198 2128 Size: 3435 437 198 4997 Fragments arranged by size: 4997 3435 437 198 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6877 278 Size: 53 83 186 936 5341 2468 Fragments arranged by size: 5341 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3264 6096 6410 1937 Size: 1327 2832 314 4594 Fragments arranged by size: 4594 2832 1327 314 Acc65I G'GTAC_C Cuts at: 4447 4489 6388 4447 Size: 42 1899 7126 Fragments arranged by size: 7126 1899 42 AclI AA'CG_TT Cuts at: 1563 7194 7567 1563 Size: 5631 373 3063 Fragments arranged by size: 5631 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2564 2582 2690 3225 3311 4223 4290 4301 Size: 932 18 108 535 86 912 67 11 Cuts at: 4301 7130 8514 8782 1632 Size: 2829 1384 268 1917 Fragments arranged by size: 2829 1917 1384 932 912 535 268 108 86 67 18 11 AfeI AGC'GCT Cuts at: 3714 5028 3714 Size: 1314 7753 AflII C'TTAA_G Cuts at: 820 1017 4250 820 Size: 197 3233 5637 Fragments arranged by size: 5637 3233 197 AflIII A'CryG_T Cuts at: 1203 3871 4532 7966 8943 1203 Size: 2668 661 3434 977 1327 Fragments arranged by size: 3434 2668 1327 977 661 AgeI A'CCGG_T Cuts at: 7898 7898 Size: 9067 AhdI GACnn_n'nnGTC Cuts at: 4951 7796 4951 Size: 2845 6222 AleI CACnn'nnGTG Cuts at: 1166 5261 1166 Size: 4095 4972 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2118 4439 5457 1184 Size: 934 2321 1018 4794 Fragments arranged by size: 4794 2321 1018 934 AlwNI CAG_nnn'CTG Cuts at: 3418 3554 4589 4973 5607 6810 8382 3418 Size: 136 1035 384 634 1203 1572 4103 Fragments arranged by size: 4103 1572 1203 1035 634 384 136 ApaI G_GGCC'C Cuts at: 3561 5765 6138 3561 Size: 2204 373 6490 Fragments arranged by size: 6490 2204 373 ApaLI G'TGCA_C Cuts at: 3212 7124 8280 3212 Size: 3912 1156 3999 Fragments arranged by size: 3999 3912 1156 ApoI r'AATT_y Cuts at: 1493 2237 4608 6376 6602 6754 8863 1493 Size: 744 2371 1768 226 152 2109 1697 Fragments arranged by size: 2371 2109 1768 1697 744 226 152 AseI AT'TA_AT Cuts at: 160 1032 2177 7621 160 Size: 872 1145 5444 1606 Fragments arranged by size: 5444 1606 1145 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9067 AvaI C'yCGr_G Cuts at: 1870 1943 3887 5123 5147 5342 5365 5414 5429 Size: 73 1944 1236 24 195 23 49 15 Cuts at: 5429 5810 6041 6392 1870 Size: 381 231 351 4545 Fragments arranged by size: 4545 1944 1236 381 351 231 195 73 49 24 23 15 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4374 4407 4374 Size: 33 9034 BamHI G'GATC_C Cuts at: 6397 6764 6397 Size: 367 8700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 4447 4489 5153 5988 Size: 325 217 627 326 2334 42 664 835 Cuts at: 5988 6388 7843 618 Size: 400 1455 1842 Fragments arranged by size: 2334 1842 1455 835 664 627 400 326 325 217 42 BanII G_rGCy'C Cuts at: 1391 3561 3896 5343 5765 6138 6386 1391 Size: 2170 335 1447 422 373 248 4072 Fragments arranged by size: 4072 2170 1447 422 373 335 248 BbeI G_GCGC'C Cuts at: 2117 2117 Size: 9067 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 2311 4422 4806 5595 928 Size: 971 393 19 2111 384 789 4400 Fragments arranged by size: 4400 2111 971 789 393 384 19 BbvCI CC'TCA_GC Cuts at: 5921 5969 5921 Size: 48 9019 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3335 3369 5693 5727 7258 Size: 34 50 34 1314 34 2324 34 1531 Cuts at: 7258 7292 1903 Size: 34 3678 Fragments arranged by size: 3678 2324 1531 1314 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2212 6959 8169 2212 Size: 4747 1210 3110 Fragments arranged by size: 4747 3110 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7678 136 Size: 107 122 71 631 605 6006 1525 Fragments arranged by size: 6006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9062 9062 Size: 9067 BlpI GC'TnA_GC Cuts at: 3447 6314 6484 3447 Size: 2867 170 6030 Fragments arranged by size: 6030 2867 170 Bme1580I G_kGCm'C Cuts at: 1790 2483 3216 3561 5073 5156 5765 5961 6138 Size: 693 733 345 1512 83 609 196 177 Cuts at: 6138 7128 8284 1790 Size: 990 1156 2573 Fragments arranged by size: 2573 1512 1156 990 733 693 609 345 196 177 83 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3936 4775 6798 Size: 416 53 83 399 24 2519 839 2023 Cuts at: 6798 7756 442 Size: 958 1753 Fragments arranged by size: 2519 2023 1753 958 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2919 3149 3627 4961 5024 5500 5629 7727 Size: 1680 230 478 1334 63 476 129 2098 Cuts at: 7727 1239 Size: 2579 Fragments arranged by size: 2579 2098 1680 1334 478 476 230 129 63 Bpu10I CC'TnA_GC Cuts at: 3419 3779 5921 5969 6167 6203 3419 Size: 360 2142 48 198 36 6283 Fragments arranged by size: 6283 2142 360 198 48 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2379 3815 7192 8057 8355 2379 Size: 1436 3377 865 298 3091 Fragments arranged by size: 3377 3091 1436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3009 3187 4673 5936 6107 7730 882 Size: 617 1510 178 1486 1263 171 1623 2219 Fragments arranged by size: 2219 1623 1510 1486 1263 617 178 171 BsaAI yAC'GTr Cuts at: 493 1204 3874 4533 6858 493 Size: 711 2670 659 2325 2702 Fragments arranged by size: 2702 2670 2325 711 659 BsaBI GATnn'nnATC Cuts at: 9061 9061 Size: 9067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2114 6874 7256 7891 Size: 53 83 186 936 581 4760 382 635 Cuts at: 7891 275 Size: 1451 Fragments arranged by size: 4760 1451 936 635 581 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3520 7500 7898 8172 8319 8730 3520 Size: 3980 398 274 147 411 3857 Fragments arranged by size: 3980 3857 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8842 Fragments arranged by size: 8842 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3307 3812 4691 4900 5372 5876 6159 1186 Size: 2121 505 879 209 472 504 283 4094 Fragments arranged by size: 4094 2121 879 505 504 472 283 209 BseYI C'CCAG_C Cuts at: 2075 2308 2350 3123 3367 3765 4524 4966 5568 Size: 233 42 773 244 398 759 442 602 Cuts at: 5568 6130 6175 6181 8270 8766 2075 Size: 562 45 6 2089 496 2376 Fragments arranged by size: 2376 2089 773 759 602 562 496 442 398 244 233 45 42 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2124 2182 2765 2774 4463 5656 1163 Size: 573 388 58 583 9 1689 1193 4574 Fragments arranged by size: 4574 1689 1193 583 573 388 58 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5665 6517 7278 8306 1540 Size: 275 186 3664 852 761 1028 2301 Fragments arranged by size: 3664 2301 1028 852 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2700 3216 4418 5952 7128 7213 8284 2700 Size: 516 1202 1534 1176 85 1071 3483 Fragments arranged by size: 3483 1534 1202 1176 1071 516 85 BsmI GAATG_Cn' Cuts at: 3332 4261 5626 6578 6671 3332 Size: 929 1365 952 93 5728 Fragments arranged by size: 5728 1365 952 929 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5267 5267 Size: 9067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2483 2700 3216 3561 3896 4418 5073 Size: 399 693 217 516 345 335 522 655 Cuts at: 5073 5156 5343 5765 5952 5961 6138 6386 7128 Size: 83 187 422 187 9 177 248 742 Cuts at: 7128 7213 8284 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 693 655 522 516 422 399 345 335 248 217 187 187 177 85 83 9 BspEI T'CCGG_A Cuts at: 8730 8730 Size: 9067 BspHI T'CATG_A Cuts at: 6956 8686 6956 Size: 1730 7337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2128 2862 3240 4009 4435 5563 5904 5919 Size: 1284 734 378 769 426 1128 341 15 Cuts at: 5919 6000 6198 6318 6422 844 Size: 81 198 120 104 3489 Fragments arranged by size: 3489 1284 1128 769 734 426 378 341 198 120 104 81 15 BsrBI CCG'CTC Cuts at: 1394 1823 5144 6171 6954 1394 Size: 429 3321 1027 783 3507 Fragments arranged by size: 3507 3321 1027 783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 6341 7562 7736 65 Size: 1183 3173 1920 1221 174 1396 Fragments arranged by size: 3173 1920 1396 1221 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2066 3363 4818 5083 5237 7711 Size: 110 207 49 1297 1455 265 154 2474 Cuts at: 7711 7898 1700 Size: 187 2869 Fragments arranged by size: 2869 2474 1455 1297 265 207 187 154 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9067 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9067 BssSI C'ACGA_G Cuts at: 3505 7127 8139 3505 Size: 3622 1012 4433 Fragments arranged by size: 4433 3622 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2779 2788 3220 5956 794 Size: 1985 9 432 2736 3905 Fragments arranged by size: 3905 2736 1985 432 9 BstBI TT'CG_AA Cuts at: 4464 7871 4464 Size: 3407 5660 BstEII G'GTnAC_C Cuts at: 1184 2663 1184 Size: 1479 7588 BstXI CCAn_nnnn'nTGG Cuts at: 5524 6130 5524 Size: 606 8461 BstYI r'GATC_y Cuts at: 1972 3550 5045 6280 6397 6764 7149 7166 8618 Size: 1578 1495 1235 117 367 385 17 1452 Cuts at: 8618 8704 8716 9062 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1578 1495 1452 1235 385 367 346 117 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3596 6187 3596 Size: 2591 6476 BtgI C'CryG_G Cuts at: 513 1065 2754 3198 5178 5583 5685 513 Size: 552 1689 444 1980 405 102 3895 Fragments arranged by size: 3895 1980 1689 552 444 405 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2938 4799 5199 5256 5655 487 Size: 683 56 1712 1861 400 57 399 3899 Fragments arranged by size: 3899 1861 1712 683 400 399 57 56 BtsI GCAGTG_nn' Cuts at: 2077 3284 6035 6583 7377 7397 2077 Size: 1207 2751 548 794 20 3747 Fragments arranged by size: 3747 2751 1207 794 548 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9032 DraI TTT'AAA Cuts at: 3997 4854 6371 3997 Size: 857 1517 6693 Fragments arranged by size: 6693 1517 857 DraIII CAC_nnn'GTG Cuts at: 5956 5956 Size: 9067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3260 8074 809 Size: 507 1944 4814 1802 Fragments arranged by size: 4814 1944 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2763 3580 5191 Size: 54 1425 50 275 18 933 817 1611 Cuts at: 5191 6018 6514 7403 9032 8 Size: 827 496 889 1629 43 Fragments arranged by size: 1629 1611 1425 933 889 827 817 496 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6514 1537 Size: 275 4702 4090 Fragments arranged by size: 4702 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6997 1353 Size: 5644 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2044 5371 7654 8028 8174 387 Size: 1064 138 455 3327 2283 374 146 1280 Fragments arranged by size: 3327 2283 1280 1064 455 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2564 2582 2690 2919 3149 3225 3311 Size: 393 932 18 108 229 230 76 86 Cuts at: 3311 3627 4223 4290 4301 4961 5024 5500 5629 Size: 316 596 67 11 660 63 476 129 Cuts at: 5629 7130 7727 8514 8782 1239 Size: 1501 597 787 268 1524 Fragments arranged by size: 1524 1501 932 787 660 597 596 476 393 316 268 230 229 129 108 86 76 67 63 18 11 EcoNI CCTnn'n_nnAGG Cuts at: 4940 5837 4940 Size: 897 8170 EcoO109I rG'GnC_Cy Cuts at: 2215 3415 3601 4650 5362 5761 5893 6134 6135 Size: 1200 186 1049 712 399 132 241 1 Cuts at: 6135 2215 Size: 5147 Fragments arranged by size: 5147 1200 1049 712 399 241 186 132 1 EcoRI G'AATT_C Cuts at: 6376 6754 6376 Size: 378 8689 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2476 2800 2476 Size: 324 8743 FspI TGC'GCA Cuts at: 2684 7573 2684 Size: 4889 4178 HaeII r_GCGC'y Cuts at: 1678 2117 3716 3779 5030 8214 1678 Size: 439 1599 63 1251 3184 2531 Fragments arranged by size: 3184 2531 1599 1251 439 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2279 2311 4634 4666 4840 Size: 32 135 32 707 32 2323 32 174 Cuts at: 4840 4872 4937 4969 5122 5154 5318 5336 5350 Size: 32 65 32 153 32 164 18 14 Cuts at: 5350 5368 6257 6289 7708 7740 7782 7814 1373 Size: 18 889 32 1419 32 42 32 2626 Fragments arranged by size: 2626 2323 1419 889 707 174 164 153 135 65 42 32 32 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3265 6339 6411 6657 669 Size: 988 281 1327 3074 72 246 3079 Fragments arranged by size: 3079 3074 1327 988 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 9067 HpaI GTT'AAC Cuts at: 6657 6657 Size: 9067 KasI G'GCGC_C Cuts at: 2113 2113 Size: 9067 KpnI G_GTAC'C Cuts at: 4451 4493 6392 4451 Size: 42 1899 7126 Fragments arranged by size: 7126 1899 42 MfeI C'AATT_G Cuts at: 6666 6666 Size: 9067 MluI A'CGCG_T Cuts at: 8943 8943 Size: 9067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2587 2853 3090 3345 3625 5913 8181 8365 Size: 1282 266 237 255 280 2288 2268 184 Cuts at: 8365 1305 Size: 2007 Fragments arranged by size: 2288 2268 2007 1282 280 266 255 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7245 Fragments arranged by size: 7245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4230 5261 7025 7384 7543 518 Size: 648 3064 1031 1764 359 159 2042 Fragments arranged by size: 3064 2042 1764 1031 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2756 3127 3200 3250 3579 5199 5285 5637 Size: 1150 371 73 50 329 1620 86 352 Cuts at: 5637 6017 6483 7160 8308 8553 1606 Size: 380 466 677 1148 245 2120 Fragments arranged by size: 2120 1620 1150 1148 677 466 380 371 352 329 245 86 73 50 NaeI GCC'GGC Cuts at: 2019 2068 4820 5085 5239 2019 Size: 49 2752 265 154 5847 Fragments arranged by size: 5847 2752 265 154 49 NarI GG'CG_CC Cuts at: 2114 2114 Size: 9067 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8515 NdeI CA'TA_TG Cuts at: 387 387 Size: 9067 NgoMIV G'CCGG_C Cuts at: 2017 2066 4818 5083 5237 2017 Size: 49 2752 265 154 5847 Fragments arranged by size: 5847 2752 265 154 49 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9067 NotI GC'GGCC_GC Cuts at: 6514 6514 Size: 9067 NspI r_CATG'y Cuts at: 1132 1229 2704 4345 6425 7970 1132 Size: 97 1475 1641 2080 1545 2229 Fragments arranged by size: 2229 2080 1641 1545 1475 97 PasI CC'CwG_GG Cuts at: 1348 5293 1348 Size: 3945 5122 PciI A'CATG_T Cuts at: 7966 7966 Size: 9067 PflMI CCAn_nnn'nTGG Cuts at: 1108 4639 1108 Size: 3531 5536 PfoI T'CCnGG_A Cuts at: 4551 4758 4938 4551 Size: 207 180 8680 Fragments arranged by size: 8680 207 180 PmeI GTTT'AAAC Cuts at: 6371 6371 Size: 9067 PmlI CAC'GTG Cuts at: 3874 4533 3874 Size: 659 8408 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7136 7168 8649 8681 7136 Size: 32 1481 32 7522 Fragments arranged by size: 7522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 3601 4650 5362 5893 2215 Size: 1386 1049 712 531 5389 Fragments arranged by size: 5389 1386 1049 712 531 PshAI GACnn'nnGTC Cuts at: 1613 4578 1613 Size: 2965 6102 PsiI TTA'TAA Cuts at: 6637 6637 Size: 9067 PspOMI G'GGCC_C Cuts at: 3557 5761 6134 3557 Size: 2204 373 6490 Fragments arranged by size: 6490 2204 373 PstI C_TGCA'G Cuts at: 830 2128 2733 2977 3243 6419 830 Size: 1298 605 244 266 3176 3478 Fragments arranged by size: 3478 3176 1298 605 266 244 PvuI CG_AT'CG Cuts at: 2001 5665 2001 Size: 3664 5403 PvuII CAG'CTG Cuts at: 3127 5285 3127 Size: 2158 6909 SacII CC_GC'GG Cuts at: 2757 3201 2757 Size: 444 8623 SalI G'TCGA_C Cuts at: 1936 3263 6409 1936 Size: 1327 3146 4594 Fragments arranged by size: 4594 3146 1327 SanDI GG'GwC_CC Cuts at: 3601 3601 Size: 9067 SbfI CC_TGCA'GG Cuts at: 6419 6419 Size: 9067 ScaI AGT'ACT Cuts at: 7315 9027 7315 Size: 1712 7355 SfcI C'TryA_G Cuts at: 826 1045 2124 2376 2729 2973 3239 6415 7550 Size: 219 1079 252 353 244 266 3176 1135 Cuts at: 7550 8231 8422 826 Size: 681 191 1471 Fragments arranged by size: 3176 1471 1135 1079 681 353 266 252 244 219 191 SfoI GGC'GCC Cuts at: 2115 2115 Size: 9067 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9067 SgrAI Cr'CCGG_yG Cuts at: 1700 2017 1700 Size: 317 8750 SmaI CCC'GGG Cuts at: 1872 5367 6394 1872 Size: 3495 1027 4545 Fragments arranged by size: 4545 3495 1027 SmlI C'TyrA_G Cuts at: 820 1017 1943 2394 3794 4250 7171 8072 8334 Size: 197 926 451 1400 456 2921 901 262 Cuts at: 8334 820 Size: 1553 Fragments arranged by size: 2921 1553 1400 926 901 456 451 262 197 SnaBI TAC'GTA Cuts at: 493 6858 493 Size: 6365 2702 SpeI A'CTAG_T Cuts at: 152 5065 152 Size: 4913 4154 SphI G_CATG'C Cuts at: 1132 6425 1132 Size: 5293 3774 SspI AAT'ATT Cuts at: 5 52 2175 2341 2593 3957 6991 5 Size: 47 2123 166 252 1364 3034 2081 Fragments arranged by size: 3034 2123 2081 1364 252 166 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9067 StyI C'CwwG_G Cuts at: 513 1065 5385 6506 513 Size: 552 4320 1121 3074 Fragments arranged by size: 4320 3074 1121 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 2999 3416 5960 7094 7111 7264 Size: 1255 1071 348 417 2544 1134 17 153 Cuts at: 7264 7449 7894 325 Size: 185 445 1498 Fragments arranged by size: 2544 1498 1255 1134 1071 445 417 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3806 3833 7313 Size: 274 80 33 51 1443 1829 27 3480 Cuts at: 7313 9025 96 Size: 1712 138 Fragments arranged by size: 3480 1829 1712 1443 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9027 9059 9027 Size: 32 9035 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9067 XbaI T'CTAG_A Cuts at: 6403 6403 Size: 9067 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3364 4661 5728 3364 Size: 1297 1067 6703 Fragments arranged by size: 6703 1297 1067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9067 XmaI C'CCGG_G Cuts at: 1870 5365 6392 1870 Size: 3495 1027 4545 Fragments arranged by size: 4545 3495 1027 XmnI GAAnn'nnTTC Cuts at: 2625 7196 2625 Size: 4571 4496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6875 276 Size: 53 83 186 936 5341 2468 Fragments arranged by size: 5341 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI