Restriction map for
pFN21AA1071
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AccI AflIII AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BbsI BciVI BglII BpuAI BsaAI
BsaBI BsiEI BsiWI BspDI BspHI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FauI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 1411 CGCG CG^CG
AccIII 1880 TCCGGA T^CCGGA
AclI 1951 AACGTT AA^CGTT
AcyI 434 GACGCC G[GA]^CG[CT]C
AflII 496 CTTAAG C^TTAAG
AgeI 3097 ACCGGT A^CCGGT
ApaI 165 GGGCCC GGGCC^C
BanI 3006 GGCACC G^G[CT][GA]CC
BcgI 2540 CGACTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2282 TGATCA T^GATCA
BfrI 496 CTTAAG C^TTAAG
BglI 686 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1237 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1237 GCTCAGC GC^T[ACGT]AGC
BsaHI 434 GACGCC G[GA]^CG[CT]C
BseAI 1880 TCCGGA T^CCGGA
BsgI 1973 CTGCAC GTGCAG(16/14)
Bsp1407I 1830 TGTACA T^GTACA
BspEI 1880 TCCGGA T^CCGGA
BsrDI 890 CATTGC GCAATG(2/0)
BsrFI 3097 ACCGGT [GA]^CCGG[CT]
BsrGI 1830 TGTACA T^GTACA
BssSI 1435 CTCGTG CACGAG(-5/-1)
BstUI 1411 CGCG CG^CG
CelII 1237 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3097 ACCGGT [GA]^CCGG[CT]
DrdI 2119 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1771 GAAGAG CTCTTC(1/4)
EcoRI 646 GAATTC G^AATTC
EcoT22I 1510 ATGCAT ATGCA^T
FbaI 2282 TGATCA T^GATCA
Hin1I 434 GACGCC G[GA]^CG[CT]C
HincII 1798 GTTGAC GT[CT]^[GA]AC
HindII 1798 GTTGAC GT[CT]^[GA]AC
Ksp632I 1771 GAAGAG CTCTTC(1/4)
MroI 1880 TCCGGA T^CCGGA
MvnI 1411 CGCG CG^CG
NcoI 2717 CCATGG C^CATGG
NdeI 1424 CATATG CA^TATG
NsiI 1510 ATGCAT ATGCA^T
PflMI 1302 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3097 ACCGGT A^CCGGT
PpuMI 952 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1951 AACGTT AA^CGTT
SacI 1984 GAGCTC GAGCT^C
SapI 1771 GAAGAGC GCTCTTC(1/4)
SmaI 162 CCCGGG CCC^GGG
SspBI 1830 TGTACA T^GTACA
SspI 2051 AATATT AAT^ATT
Van91I 1302 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 162 CCCGGG C^CCGGG
XmaI 162 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 6 AAATTC [GA]^AATT[CT]
646 GAATTC
ApoI 6 AAATTC [GA]^AATT[CT]
646 GAATTC
AvaI 162 CCCGGG C^[CT]CG[GA]G
1232 CTCGGG
AvrII 1048 CCTAGG C^CTAGG
2469 CCTAGG
BbvCI 2861 CCTCAGC CCTCAGC(-5/-2)
3073 GCTGAGG
BlnI 1048 CCTAGG C^CTAGG
2469 CCTAGG
BsaI 491 GAGACC GGTCTC(1/5)
1028 GAGACC
BsmI 296 GAATGC GAATGC(1/-1)
2474 GCATTC
BsoBI 162 CCCGGG C^[CT]CG[GA]G
1232 CTCGGG
BspMI 1043 ACCTGC ACCTGC(4/8)
3136 ACCTGC
BstAPI 45 GCAGCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2626 GCAGCTGTTGC
BstXI 2711 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3176 CCAATACTCTGG
CfoI 877 GCGC GCG^C
1410 GCGC
DsaI 35 CCACGG C^C[GA][CT]GG
2717 CCATGG
HhaI 877 GCGC GCG^C
1410 GCGC
HinP1I 877 GCGC G^CGC
1410 GCGC
ScaI 350 AGTACT AGT^ACT
2289 AGTACT
Tsp509I 7 AATT ^AATT
647 AATT
XbaI 1542 TCTAGA T^CTAGA
2166 TCTAGA
XcmI 2592 CCAAACTGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3200 CCATTCAGATCCTGG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I XhoII