Restriction map for
pFN21AA1071
(Linear) (Six-base) MAPSORT of: KIAA1071.seq Check: 3379 from: 1 to: 3252
KIAA1071.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1052 3252
Size: 1052 2200
AclI AA'CG_TT
Cuts at: 0 1952 3252
Size: 1952 1300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 709 2999 3252
Size: 32 677 2290 253
Fragments arranged by size:
2290 677 253 32
AflII C'TTAA_G
Cuts at: 0 496 3252
Size: 496 2756
AgeI A'CCGG_T
Cuts at: 0 3097 3252
Size: 3097 155
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1637 3252
Size: 1637 1615
AlwNI CAG_nnn'CTG
Cuts at: 0 1734 2662 2740 2767 2791 2827 2848 2869
Size: 1734 928 78 27 24 36 21 21
Cuts at: 2869 3210 3252
Size: 341 42
Fragments arranged by size:
1734 928 341 78 42 36 27 24
21 21
ApaI G_GGCC'C
Cuts at: 0 169 3252
Size: 169 3083
ApoI r'AATT_y
Cuts at: 0 6 646 3252
Size: 6 640 2606
Fragments arranged by size:
2606 640 6
AvaI C'yCGr_G
Cuts at: 0 162 1232 3252
Size: 162 1070 2020
Fragments arranged by size:
2020 1070 162
AvrII C'CTAG_G
Cuts at: 0 1048 2469 3252
Size: 1048 1421 783
Fragments arranged by size:
1421 1048 783
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2571 2604 3252
Size: 2571 33 648
Fragments arranged by size:
2571 648 33
BanI G'GyrC_C
Cuts at: 0 3006 3252
Size: 3006 246
BanII G_rGCy'C
Cuts at: 0 169 1239 1302 1988 3252
Size: 169 1070 63 686 1264
Fragments arranged by size:
1264 1070 686 169 63
BbvCI CC'TCA_GC
Cuts at: 0 2862 3074 3252
Size: 2862 212 178
Fragments arranged by size:
2862 212 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2529 2563 3252
Size: 2529 34 689
Fragments arranged by size:
2529 689 34
BclI T'GATC_A
Cuts at: 0 2282 3252
Size: 2282 970
BfrBI ATG'CAT
Cuts at: 0 1512 3252
Size: 1512 1740
BglI GCCn_nnn'nGGC
Cuts at: 0 692 3252
Size: 692 2560
BlpI GC'TnA_GC
Cuts at: 0 1238 3252
Size: 1238 2014
Bme1580I G_kGCm'C
Cuts at: 0 169 782 3252
Size: 169 613 2470
Fragments arranged by size:
2470 613 169
BmrI ACTGGGnnnn_n'
Cuts at: 0 140 342 3252
Size: 140 202 2910
Fragments arranged by size:
2910 202 140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1338 3252
Size: 1338 1914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 1822 1912 2278 2639 2717 2744 2768
Size: 422 1400 90 366 361 78 27 24
Cuts at: 2768 2804 2900 2915 2951 3023 3041 3252
Size: 36 96 15 36 72 18 211
Fragments arranged by size:
1400 422 366 361 211 96 90 78
72 36 36 27 24 18 15
Bpu10I CC'TnA_GC
Cuts at: 0 2862 2978 3068 3074 3252
Size: 2862 116 90 6 178
Fragments arranged by size:
2862 178 116 90 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1362 3252
Size: 540 822 1890
Fragments arranged by size:
1890 822 540
BsaI GGTCTCn'nnnn_
Cuts at: 0 485 1022 3252
Size: 485 537 2230
Fragments arranged by size:
2230 537 485
BsaHI Gr'CG_yC
Cuts at: 0 435 3252
Size: 435 2817
BsaWI w'CCGG_w
Cuts at: 0 1334 1880 2955 3097 3252
Size: 1334 546 1075 142 155
Fragments arranged by size:
1334 1075 546 155 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 573 603 1090 1120 3003 3027 3033 3039
Size: 573 30 487 30 1883 24 6 6
Cuts at: 3039 3057 3069 3252
Size: 18 12 183
Fragments arranged by size:
1883 573 487 183 30 30 24 18
12 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 281 2017 2074 2203 3252
Size: 281 1736 57 129 1049
Fragments arranged by size:
1736 1049 281 129 57
BseYI C'CCAG_C
Cuts at: 0 1301 3252
Size: 1301 1951
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1958 3252
Size: 1958 1294
BsiHKAI G_wGCw'C
Cuts at: 0 591 1874 1988 3252
Size: 591 1283 114 1264
Fragments arranged by size:
1283 1264 591 114
BsmI GAATG_Cn'
Cuts at: 0 302 2474 3252
Size: 302 2172 778
Fragments arranged by size:
2172 778 302
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1108 1419 1882 2438 2786 3252
Size: 1108 311 463 556 348 466
Fragments arranged by size:
1108 556 466 463 348 311
Bsp1286I G_dGCh'C
Cuts at: 0 169 591 782 1239 1302 1874 1988 3252
Size: 169 422 191 457 63 572 114 1264
Fragments arranged by size:
1264 572 457 422 191 169 114 63
BspEI T'CCGG_A
Cuts at: 0 1880 3252
Size: 1880 1372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1052 3145 3252
Size: 1052 2093 107
Fragments arranged by size:
2093 1052 107
BsrDI GCAATG_nn'
Cuts at: 0 889 3252
Size: 889 2363
BsrFI r'CCGG_y
Cuts at: 0 3097 3252
Size: 3097 155
BsrGI T'GTAC_A
Cuts at: 0 1830 3252
Size: 1830 1422
BssSI C'ACGA_G
Cuts at: 0 1435 3252
Size: 1435 1817
BstAPI GCAn_nnn'nTGC
Cuts at: 0 51 2632 3252
Size: 51 2581 620
Fragments arranged by size:
2581 620 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2718 3183 3252
Size: 2718 465 69
Fragments arranged by size:
2718 465 69
BstYI r'GATC_y
Cuts at: 0 1316 1527 3206 3252
Size: 1316 211 1679 46
Fragments arranged by size:
1679 1316 211 46
BtgI C'CryG_G
Cuts at: 0 35 2717 3252
Size: 35 2682 535
Fragments arranged by size:
2682 535 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 781 3252
Size: 781 2471
BtsI GCAGTG_nn'
Cuts at: 0 159 1676 2672 2687 2693 2789 3252
Size: 159 1517 996 15 6 96 463
Fragments arranged by size:
1517 996 463 159 96 15 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3098 3133 3252
Size: 3098 35 119
Fragments arranged by size:
3098 119 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2125 3252
Size: 2125 1127
EaeI y'GGCC_r
Cuts at: 0 548 1673 2207 2347 3252
Size: 548 1125 534 140 905
Fragments arranged by size:
1125 905 548 534 140
EarI CTCTTCn'nnn_
Cuts at: 0 1766 3252
Size: 1766 1486
EcoICRI GAG'CTC
Cuts at: 0 1986 3252
Size: 1986 1266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 422 709 1822 1912 2278 2639 2717
Size: 32 390 287 1113 90 366 361 78
Cuts at: 2717 2744 2768 2804 2900 2915 2951 2999 3023
Size: 27 24 36 96 15 36 48 24
Cuts at: 3023 3041 3252
Size: 18 211
Fragments arranged by size:
1113 390 366 361 287 211 96 90
78 48 36 36 32 27 24 24
18 15
EcoO109I rG'GnC_Cy
Cuts at: 0 166 709 953 1461 1731 1824 3252
Size: 166 543 244 508 270 93 1428
Fragments arranged by size:
1428 543 508 270 244 166 93
EcoRI G'AATT_C
Cuts at: 0 646 3252
Size: 646 2606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 82 685 3252
Size: 82 603 2567
Fragments arranged by size:
2567 603 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 825 857 1524 1556 1697 1729 2001 2033
Size: 825 32 667 32 141 32 272 32
Cuts at: 2033 2320 2352 3126 3158 3252
Size: 287 32 774 32 94
Fragments arranged by size:
825 774 667 287 272 141 94 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1800 3252
Size: 1800 1452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1334 3058 3070 3076 3252
Size: 360 974 1724 12 6 176
Fragments arranged by size:
1724 974 360 176 12 6
MscI TGG'CCA
Cuts at: 0 550 1675 2209 3252
Size: 550 1125 534 1043
Fragments arranged by size:
1125 1043 550 534
MslI CAynn'nnrTG
Cuts at: 0 374 476 2716 3252
Size: 374 102 2240 536
Fragments arranged by size:
2240 536 374 102
MspA1I CmG'CkG
Cuts at: 0 285 450 2334 2629 2764 2797 2824 2845
Size: 285 165 1884 295 135 33 27 21
Cuts at: 2845 2866 2920 2974 3061 3252
Size: 21 54 54 87 191
Fragments arranged by size:
1884 295 285 191 165 135 87 54
54 33 27 21 21
NcoI C'CATG_G
Cuts at: 0 2717 3252
Size: 2717 535
NdeI CA'TA_TG
Cuts at: 0 1425 3252
Size: 1425 1827
NsiI A_TGCA'T
Cuts at: 0 1514 3252
Size: 1514 1738
NspI r_CATG'y
Cuts at: 0 373 511 741 1850 2346 3252
Size: 373 138 230 1109 496 906
Fragments arranged by size:
1109 906 496 373 230 138
PasI CC'CwG_GG
Cuts at: 0 240 3252
Size: 240 3012
PflMI CCAn_nnn'nTGG
Cuts at: 0 1308 3252
Size: 1308 1944
PfoI T'CCnGG_A
Cuts at: 0 459 2305 3102 3252
Size: 459 1846 797 150
Fragments arranged by size:
1846 797 459 150
PpuMI rG'GwC_Cy
Cuts at: 0 953 3252
Size: 953 2299
PspOMI G'GGCC_C
Cuts at: 0 165 3252
Size: 165 3087
PstI C_TGCA'G
Cuts at: 0 1388 2587 2628 3252
Size: 1388 1199 41 624
Fragments arranged by size:
1388 1199 624 41
PvuII CAG'CTG
Cuts at: 0 285 2334 2629 2764 2824 2845 2866 2920
Size: 285 2049 295 135 60 21 21 54
Cuts at: 2920 3061 3252
Size: 141 191
Fragments arranged by size:
2049 295 285 191 141 135 60 54
21 21
SacI G_AGCT'C
Cuts at: 0 1988 3252
Size: 1988 1264
SapI GCTCTTCn'nnn_
Cuts at: 0 1766 3252
Size: 1766 1486
ScaI AGT'ACT
Cuts at: 0 352 2291 3252
Size: 352 1939 961
Fragments arranged by size:
1939 961 352
SfcI C'TryA_G
Cuts at: 0 752 786 1384 2583 2624 3252
Size: 752 34 598 1199 41 628
Fragments arranged by size:
1199 752 628 598 41 34
SmaI CCC'GGG
Cuts at: 0 164 3252
Size: 164 3088
SmlI C'TyrA_G
Cuts at: 0 496 519 1341 3252
Size: 496 23 822 1911
Fragments arranged by size:
1911 822 496 23
SphI G_CATG'C
Cuts at: 0 373 741 2346 3252
Size: 373 368 1605 906
Fragments arranged by size:
1605 906 373 368
SspI AAT'ATT
Cuts at: 0 2053 3252
Size: 2053 1199
StyI C'CwwG_G
Cuts at: 0 335 660 1010 1048 1751 2469 2717 3252
Size: 335 325 350 38 703 718 248 535
Fragments arranged by size:
718 703 535 350 335 325 248 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2473 3252
Size: 2473 779
TatI w'GTAC_w
Cuts at: 0 350 1830 2289 3252
Size: 350 1480 459 963
Fragments arranged by size:
1480 963 459 350
XbaI T'CTAG_A
Cuts at: 0 1542 2166 3252
Size: 1542 624 1086
Fragments arranged by size:
1542 1086 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2599 3207 3252
Size: 2599 608 45
Fragments arranged by size:
2599 608 45
XmaI C'CCGG_G
Cuts at: 0 162 3252
Size: 162 3090
Enzymes that do cut:
AarI AclI AcuI AflII AgeI AlfI AlwNI ApaI
ApoI AvaI AvrII BaeI BanI BanII BbvCI BcgI
BclI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI Hin4I HincII MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SapI ScaI SfcI SmaI
SmlI SphI SspI StyI TaqII TatI XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflIII AhdI AleI AloI
ApaLI AscI AseI AsiSI BamHI BbeI BbsI BciVI
BglII BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BspHI
BsrBI BssHII BstBI BstEII BstZ17I Bsu36I ClaI DraI
DraIII EagI EciI EcoNI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI StuI SwaI TstI Tth111I
XhoI XmnI ZraI