(Circular) (Six-base) MAPSORT of: pFN21AA1016.seq Check: 4743 from: 1 to: 6889 pFN21AA1016 6889 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4699 278 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3796 4232 1937 Size: 1859 436 4594 Fragments arranged by size: 4594 1859 436 Acc65I G'GTAC_C Cuts at: 4210 4210 Size: 6889 AclI AA'CG_TT Cuts at: 1563 5016 5389 1563 Size: 3453 373 3063 Fragments arranged by size: 3453 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3665 4952 6336 6604 1632 Size: 2033 1287 1384 268 1917 Fragments arranged by size: 2033 1917 1384 1287 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6692 AflIII A'CryG_T Cuts at: 1203 5788 6765 1203 Size: 4585 977 1327 Fragments arranged by size: 4585 1327 977 AgeI A'CCGG_T Cuts at: 5720 5720 Size: 6889 AhdI GACnn_n'nnGTC Cuts at: 5618 5618 Size: 6889 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6889 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2753 2845 1184 Size: 1569 92 5228 Fragments arranged by size: 5228 1569 92 AlwNI CAG_nnn'CTG Cuts at: 2437 3986 4632 6204 2437 Size: 1549 646 1572 3122 Fragments arranged by size: 3122 1572 1549 646 ApaLI G'TGCA_C Cuts at: 3296 4946 6102 3296 Size: 1650 1156 4083 Fragments arranged by size: 4083 1650 1156 ApoI r'AATT_y Cuts at: 1493 2258 2373 3128 3140 3233 3679 4198 4424 Size: 765 115 755 12 93 446 519 226 Cuts at: 4424 4576 6685 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 765 755 519 446 226 152 115 93 12 AseI AT'TA_AT Cuts at: 160 1032 5443 160 Size: 872 4411 1606 Fragments arranged by size: 4411 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 AvaI C'yCGr_G Cuts at: 1870 1943 2279 4064 4214 1870 Size: 73 336 1785 150 4545 Fragments arranged by size: 4545 1785 336 150 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2380 2413 2380 Size: 33 6856 BamHI G'GATC_C Cuts at: 4219 4586 4219 Size: 367 6522 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 4210 5665 618 Size: 325 217 627 336 2087 1455 1842 Fragments arranged by size: 2087 1842 1455 627 336 325 217 BanII G_rGCy'C Cuts at: 1391 4208 1391 Size: 2817 4072 BbeI G_GCGC'C Cuts at: 2127 2127 Size: 6889 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3036 3267 3810 928 Size: 971 1137 231 543 4007 Fragments arranged by size: 4007 1137 971 543 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5080 5114 1903 Size: 34 3143 34 3678 Fragments arranged by size: 3678 3143 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4781 5991 4781 Size: 1210 5679 BclI T'GATC_A Cuts at: 1548 1548 Size: 6889 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5500 136 Size: 107 122 71 631 605 3828 1525 Fragments arranged by size: 3828 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2698 6884 2698 Size: 4186 2703 BlpI GC'TnA_GC Cuts at: 4306 4306 Size: 6889 Bme1580I G_kGCm'C Cuts at: 1790 3300 4950 6106 1790 Size: 1510 1650 1156 2573 Fragments arranged by size: 2573 1650 1510 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6889 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4620 5578 442 Size: 416 53 83 399 24 3203 958 1753 Fragments arranged by size: 3203 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6889 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2802 3530 4087 5549 1239 Size: 974 589 728 557 1462 2579 Fragments arranged by size: 2579 1462 974 728 589 557 Bpu10I CC'TnA_GC Cuts at: 2237 3110 2237 Size: 873 6016 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2225 2473 2813 5014 5879 6177 2225 Size: 248 340 2201 865 298 2937 Fragments arranged by size: 2937 2201 865 340 298 248 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3189 3227 5552 882 Size: 617 1690 38 2325 2219 Fragments arranged by size: 2325 2219 1690 617 38 BsaAI yAC'GTr Cuts at: 493 1204 2944 4680 493 Size: 711 1740 1736 2702 Fragments arranged by size: 2702 1740 1736 711 BsaBI GATnn'nnATC Cuts at: 6883 6883 Size: 6889 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2124 4696 5078 Size: 53 83 186 936 479 112 2572 382 Cuts at: 5078 5713 275 Size: 635 1451 Fragments arranged by size: 2572 1451 936 635 479 382 186 112 83 53 BsaWI w'CCGG_w Cuts at: 3148 3520 3711 5322 5720 5994 6141 6552 3148 Size: 372 191 1611 398 274 147 411 3485 Fragments arranged by size: 3485 1611 411 398 372 274 191 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3083 3113 1178 Size: 30 165 30 1680 30 4954 Fragments arranged by size: 4954 1680 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2805 1186 Size: 1036 583 5270 Fragments arranged by size: 5270 1036 583 BseYI C'CCAG_C Cuts at: 2616 6092 6588 2616 Size: 3476 496 2917 Fragments arranged by size: 3476 2917 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 2324 3471 1163 Size: 573 312 276 1147 4581 Fragments arranged by size: 4581 1147 573 312 276 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3872 4339 5100 6128 1540 Size: 275 186 1871 467 761 1028 2301 Fragments arranged by size: 2301 1871 1028 761 467 275 186 BsiHKAI G_wGCw'C Cuts at: 2742 3300 4950 5035 6106 2742 Size: 558 1650 85 1071 3525 Fragments arranged by size: 3525 1650 1071 558 85 BsmI GAATG_Cn' Cuts at: 4180 4400 4493 4180 Size: 220 93 6576 Fragments arranged by size: 6576 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2742 3300 4208 4950 5035 6106 1391 Size: 399 952 558 908 742 85 1071 2174 Fragments arranged by size: 2174 1071 952 908 742 558 399 85 BspEI T'CCGG_A Cuts at: 3711 6552 3711 Size: 2841 4048 BspHI T'CATG_A Cuts at: 4778 6508 4778 Size: 1730 5159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 4244 844 Size: 1337 2063 3489 Fragments arranged by size: 3489 2063 1337 BsrBI CCG'CTC Cuts at: 1394 1823 4101 4776 1394 Size: 429 2278 675 3507 Fragments arranged by size: 3507 2278 675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3360 5384 5558 65 Size: 1183 2112 2024 174 1396 Fragments arranged by size: 2112 2024 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 5533 5720 1700 Size: 110 310 3413 187 2869 Fragments arranged by size: 3413 2869 310 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6889 BssHII G'CGCG_C Cuts at: 1925 2197 2199 1925 Size: 272 2 6615 Fragments arranged by size: 6615 272 2 BssSI C'ACGA_G Cuts at: 2741 3097 4949 5961 2741 Size: 356 1852 1012 3669 Fragments arranged by size: 3669 1852 1012 356 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6889 BstBI TT'CG_AA Cuts at: 4036 5693 4036 Size: 1657 5232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6889 BstYI r'GATC_y Cuts at: 1972 2698 3394 4219 4586 4971 4988 6440 6526 Size: 726 696 825 367 385 17 1452 86 Cuts at: 6526 6538 6884 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 825 726 696 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2412 2412 Size: 6889 BtgI C'CryG_G Cuts at: 513 1065 2004 2110 2916 3866 513 Size: 552 939 106 806 950 3536 Fragments arranged by size: 3536 950 939 806 552 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3089 487 Size: 683 56 1863 4287 Fragments arranged by size: 4287 1863 683 56 BtsI GCAGTG_nn' Cuts at: 4405 5199 5219 4405 Size: 794 20 6075 Fragments arranged by size: 6075 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6889 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6854 DraI TTT'AAA Cuts at: 3438 4193 3438 Size: 755 6134 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5896 809 Size: 507 4580 1802 Fragments arranged by size: 4580 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 3921 4336 Size: 54 1425 50 275 18 384 1707 415 Cuts at: 4336 5225 6854 8 Size: 889 1629 43 Fragments arranged by size: 1707 1629 1425 889 415 384 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4336 1537 Size: 275 2524 4090 Fragments arranged by size: 4090 2524 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2544 3264 3282 3732 4819 1353 Size: 1191 720 18 450 1087 3423 Fragments arranged by size: 3423 1191 1087 720 450 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2461 3851 5476 5850 5996 387 Size: 1064 138 872 1390 1625 374 146 1280 Fragments arranged by size: 1625 1390 1280 1064 872 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2802 3530 3665 4087 4952 5549 Size: 393 581 589 728 135 422 865 597 Cuts at: 5549 6336 6604 1239 Size: 787 268 1524 Fragments arranged by size: 1524 865 787 728 597 589 581 422 393 268 135 EcoO109I rG'GnC_Cy Cuts at: 4108 4108 Size: 6889 EcoRI G'AATT_C Cuts at: 4198 4576 4198 Size: 378 6511 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2737 2737 Size: 6889 FspI TGC'GCA Cuts at: 5395 5395 Size: 6889 HaeII r_GCGC'y Cuts at: 1678 2127 2244 6036 1678 Size: 449 117 3792 2531 Fragments arranged by size: 3792 2531 449 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2446 2478 3774 3806 3980 Size: 32 135 32 874 32 1296 32 174 Cuts at: 3980 4012 5530 5562 5604 5636 1373 Size: 32 1518 32 42 32 2626 Fragments arranged by size: 2626 1518 1296 874 174 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3853 4048 4233 4479 669 Size: 988 281 1915 195 185 246 3079 Fragments arranged by size: 3079 1915 988 281 246 195 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 6889 HpaI GTT'AAC Cuts at: 4479 4479 Size: 6889 KasI G'GCGC_C Cuts at: 2123 2123 Size: 6889 KpnI G_GTAC'C Cuts at: 4214 4214 Size: 6889 MfeI C'AATT_G Cuts at: 4488 4488 Size: 6889 MluI A'CGCG_T Cuts at: 6765 6765 Size: 6889 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2959 3999 6003 6187 1305 Size: 1654 1040 2004 184 2007 Fragments arranged by size: 2007 2004 1654 1040 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3923 10 Size: 54 1425 343 2091 2976 Fragments arranged by size: 2976 2091 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3776 4173 4847 5206 5365 518 Size: 648 2610 397 674 359 159 2042 Fragments arranged by size: 2610 2042 674 648 397 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 2467 2592 2776 2894 3367 3508 3902 Size: 554 307 125 184 118 473 141 394 Cuts at: 3902 4305 4982 6130 6375 1606 Size: 403 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 554 473 403 394 307 245 184 141 125 118 NarI GG'CG_CC Cuts at: 2124 2124 Size: 6889 NcoI C'CATG_G Cuts at: 513 1065 2004 2916 513 Size: 552 939 912 4486 Fragments arranged by size: 4486 939 912 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6889 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6889 NotI GC'GGCC_GC Cuts at: 4336 4336 Size: 6889 NspI r_CATG'y Cuts at: 1132 1229 2880 4247 5792 1132 Size: 97 1651 1367 1545 2229 Fragments arranged by size: 2229 1651 1545 1367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6889 PciI A'CATG_T Cuts at: 5788 5788 Size: 6889 PflMI CCAn_nnn'nTGG Cuts at: 1108 2917 2944 1108 Size: 1809 27 5053 Fragments arranged by size: 5053 1809 27 PmeI GTTT'AAAC Cuts at: 4193 4193 Size: 6889 PmlI CAC'GTG Cuts at: 2944 2944 Size: 6889 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4958 4990 6471 6503 4958 Size: 32 1481 32 5344 Fragments arranged by size: 5344 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4108 4108 Size: 6889 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6889 PsiI TTA'TAA Cuts at: 4459 4459 Size: 6889 PstI C_TGCA'G Cuts at: 830 2433 2811 3607 4017 4241 830 Size: 1603 378 796 410 224 3478 Fragments arranged by size: 3478 1603 796 410 378 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6889 PvuII CAG'CTG Cuts at: 2467 2592 2776 2894 3367 3508 2467 Size: 125 184 118 473 141 5848 Fragments arranged by size: 5848 473 184 141 125 118 SalI G'TCGA_C Cuts at: 1936 4231 1936 Size: 2295 4594 SapI GCTCTTCn'nnn_ Cuts at: 3264 3264 Size: 6889 SbfI CC_TGCA'GG Cuts at: 4241 4241 Size: 6889 ScaI AGT'ACT Cuts at: 5137 6849 5137 Size: 1712 5177 SfcI C'TryA_G Cuts at: 826 1045 2429 2807 3603 3690 4013 4237 5372 Size: 219 1384 378 796 87 323 224 1135 Cuts at: 5372 6053 6244 826 Size: 681 191 1471 Fragments arranged by size: 1471 1384 1135 796 681 378 323 224 219 191 87 SfoI GGC'GCC Cuts at: 2125 2125 Size: 6889 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6889 SmaI CCC'GGG Cuts at: 1872 2281 4216 1872 Size: 409 1935 4545 Fragments arranged by size: 4545 1935 409 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 2452 2792 4993 5894 6156 Size: 197 926 261 248 340 2201 901 262 Cuts at: 6156 820 Size: 1553 Fragments arranged by size: 2201 1553 926 901 340 262 261 248 197 SnaBI TAC'GTA Cuts at: 493 4680 493 Size: 4187 2702 SpeI A'CTAG_T Cuts at: 152 2692 152 Size: 2540 4349 SphI G_CATG'C Cuts at: 1132 2880 4247 1132 Size: 1748 1367 3774 Fragments arranged by size: 3774 1748 1367 SspI AAT'ATT Cuts at: 5 52 4813 5 Size: 47 4761 2081 Fragments arranged by size: 4761 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6889 StyI C'CwwG_G Cuts at: 513 1065 2004 2706 2916 4328 513 Size: 552 939 702 210 1412 3074 Fragments arranged by size: 3074 1412 939 702 552 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2082 3155 4916 4933 5086 5271 5716 Size: 1255 502 1073 1761 17 153 185 445 Cuts at: 5716 325 Size: 1498 Fragments arranged by size: 1761 1498 1255 1073 502 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5135 6847 96 Size: 274 80 33 51 1443 3158 1712 138 Fragments arranged by size: 3158 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3083 3115 6849 6881 3083 Size: 32 3734 32 3091 Fragments arranged by size: 3734 3091 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6889 XbaI T'CTAG_A Cuts at: 4225 4225 Size: 6889 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3916 3916 Size: 6889 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6889 XmaI C'CCGG_G Cuts at: 1870 2279 4214 1870 Size: 409 1935 4545 Fragments arranged by size: 4545 1935 409 XmnI GAAnn'nnTTC Cuts at: 3759 5018 3759 Size: 1259 5630 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4697 276 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI