(Circular) (Six-base) MAPSORT of: pFN21AA1012.seq Check: 8016 from: 1 to: 9010 pFN21AA1012 9010 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6820 278 Size: 53 83 186 936 5284 2468 Fragments arranged by size: 5284 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5010 6353 1937 Size: 3073 1343 4594 Fragments arranged by size: 4594 3073 1343 Acc65I G'GTAC_C Cuts at: 6331 6331 Size: 9010 AclI AA'CG_TT Cuts at: 1563 7137 7510 1563 Size: 5574 373 3063 Fragments arranged by size: 5574 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2381 2888 4443 7073 8457 8725 1632 Size: 749 507 1555 2630 1384 268 1917 Fragments arranged by size: 2630 1917 1555 1384 749 507 268 AflII C'TTAA_G Cuts at: 820 1017 2132 820 Size: 197 1115 7698 Fragments arranged by size: 7698 1115 197 AflIII A'CryG_T Cuts at: 1203 7909 8886 1203 Size: 6706 977 1327 Fragments arranged by size: 6706 1327 977 AgeI A'CCGG_T Cuts at: 7841 7841 Size: 9010 AhdI GACnn_n'nnGTC Cuts at: 7739 7739 Size: 9010 AleI CACnn'nnGTG Cuts at: 1166 2532 1166 Size: 1366 7644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3661 1184 Size: 2477 6533 AlwNI CAG_nnn'CTG Cuts at: 3410 3569 4928 6753 8325 3410 Size: 159 1359 1825 1572 4095 Fragments arranged by size: 4095 1825 1572 1359 159 ApaLI G'TGCA_C Cuts at: 3618 7067 8223 3618 Size: 3449 1156 4405 Fragments arranged by size: 4405 3449 1156 ApoI r'AATT_y Cuts at: 1493 2203 2423 2520 2818 3045 4641 4811 5360 Size: 710 220 97 298 227 1596 170 549 Cuts at: 5360 5867 6274 6319 6545 6697 8806 1493 Size: 507 407 45 226 152 2109 1697 Fragments arranged by size: 2109 1697 1596 710 549 507 407 298 227 226 220 170 152 97 45 AseI AT'TA_AT Cuts at: 160 1032 2688 3213 3813 3891 4446 7564 160 Size: 872 1656 525 600 78 555 3118 1606 Fragments arranged by size: 3118 1656 1606 872 600 555 525 78 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9010 AvaI C'yCGr_G Cuts at: 1870 1943 6335 1870 Size: 73 4392 4545 Fragments arranged by size: 4545 4392 73 AvrII C'CTAG_G Cuts at: 3434 6218 3434 Size: 2784 6226 BamHI G'GATC_C Cuts at: 6340 6707 6340 Size: 367 8643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3386 3828 6331 7786 618 Size: 325 217 627 1599 442 2503 1455 1842 Fragments arranged by size: 2503 1842 1599 1455 627 442 325 217 BanII G_rGCy'C Cuts at: 1391 2896 4550 6329 1391 Size: 1505 1654 1779 4072 Fragments arranged by size: 4072 1779 1654 1505 BbeI G_GCGC'C Cuts at: 3832 3832 Size: 9010 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5760 5936 928 Size: 971 3861 176 4002 Fragments arranged by size: 4002 3861 971 176 BbvCI CC'TCA_GC Cuts at: 2114 2244 4921 2114 Size: 130 2677 6203 Fragments arranged by size: 6203 2677 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7201 7235 1903 Size: 34 5264 34 3678 Fragments arranged by size: 5264 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3255 3454 6902 8112 3255 Size: 199 3448 1210 4153 Fragments arranged by size: 4153 3448 1210 199 BclI T'GATC_A Cuts at: 1548 3034 3361 4099 6291 1548 Size: 1486 327 738 2192 4267 Fragments arranged by size: 4267 2192 1486 738 327 BfrBI ATG'CAT Cuts at: 3686 3686 Size: 9010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7621 136 Size: 107 122 71 631 605 5949 1525 Fragments arranged by size: 5949 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2960 9005 2960 Size: 6045 2965 BlpI GC'TnA_GC Cuts at: 6427 6427 Size: 9010 Bme1580I G_kGCm'C Cuts at: 1790 3622 7071 8227 1790 Size: 1832 3449 1156 2573 Fragments arranged by size: 3449 2573 1832 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2005 6741 7699 Size: 416 53 83 399 24 588 4736 958 Cuts at: 7699 442 Size: 1753 Fragments arranged by size: 4736 1753 958 588 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9010 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3171 3171 Size: 9010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2271 2735 7670 1239 Size: 1032 464 4935 2579 Fragments arranged by size: 4935 2579 1032 464 Bpu10I CC'TnA_GC Cuts at: 2114 2244 4921 2114 Size: 130 2677 6203 Fragments arranged by size: 6203 2677 130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4163 4627 5884 7135 8000 8298 4163 Size: 464 1257 1251 865 298 4875 Fragments arranged by size: 4875 1257 1251 865 464 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3798 7673 882 Size: 617 2299 3875 2219 Fragments arranged by size: 3875 2299 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2194 5114 6801 493 Size: 711 990 2920 1687 2702 Fragments arranged by size: 2920 2702 1687 990 711 BsaBI GATnn'nnATC Cuts at: 3606 6296 9004 3606 Size: 2690 2708 3612 Fragments arranged by size: 3612 2708 2690 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3829 6817 7199 7834 Size: 53 83 186 936 2296 2988 382 635 Cuts at: 7834 275 Size: 1451 Fragments arranged by size: 2988 2296 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2259 7443 7841 8115 8262 8673 2259 Size: 5184 398 274 147 411 2596 Fragments arranged by size: 5184 2596 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4410 4440 5076 5106 1178 Size: 30 165 30 3007 30 636 30 5082 Fragments arranged by size: 5082 3007 636 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5310 1186 Size: 4124 4886 BseYI C'CCAG_C Cuts at: 2235 8213 8709 2235 Size: 5978 496 2536 Fragments arranged by size: 5978 2536 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 2095 2625 3323 5114 5610 1163 Size: 573 312 47 530 698 1791 496 4563 Fragments arranged by size: 4563 1791 698 573 530 496 312 47 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6460 7221 8249 1540 Size: 275 186 4459 761 1028 2301 Fragments arranged by size: 4459 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2896 3433 3622 4576 7071 7156 8227 2896 Size: 537 189 954 2495 85 1071 3679 Fragments arranged by size: 3679 2495 1071 954 537 189 85 BsmI GAATG_Cn' Cuts at: 6521 6614 6521 Size: 93 8917 BsmBI CGTCTCn'nnnn_ Cuts at: 2108 2108 Size: 9010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2896 3433 3622 4550 4576 6329 7071 Size: 399 1106 537 189 928 26 1753 742 Cuts at: 7071 7156 8227 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1753 1106 1071 928 742 537 399 189 85 26 BspEI T'CCGG_A Cuts at: 2259 8673 2259 Size: 6414 2596 BspHI T'CATG_A Cuts at: 2419 6899 8629 2419 Size: 4480 1730 2800 Fragments arranged by size: 4480 2800 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6365 844 Size: 5521 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2092 6897 1394 Size: 429 269 4805 3507 Fragments arranged by size: 4805 3507 429 269 BsrDI GCAATG_nn' Cuts at: 65 1248 3375 3643 4328 7505 7679 65 Size: 1183 2127 268 685 3177 174 1396 Fragments arranged by size: 3177 2127 1396 1183 685 268 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7654 7841 1700 Size: 110 5844 187 2869 Fragments arranged by size: 5844 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2017 4979 96 Size: 1921 2962 4127 Fragments arranged by size: 4127 2962 1921 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9010 BssSI C'ACGA_G Cuts at: 7070 8082 7070 Size: 1012 7998 BstAPI GCAn_nnn'nTGC Cuts at: 794 3386 3410 794 Size: 2592 24 6394 Fragments arranged by size: 6394 2592 24 BstBI TT'CG_AA Cuts at: 3613 7814 3613 Size: 4201 4809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9010 BstXI CCAn_nnnn'nTGG Cuts at: 2736 2736 Size: 9010 BstYI r'GATC_y Cuts at: 1972 2173 2731 2960 4714 6340 6707 7092 7109 Size: 201 558 229 1754 1626 367 385 17 Cuts at: 7109 8561 8647 8659 9005 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1754 1626 1452 558 385 367 346 229 201 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2366 3669 6097 513 Size: 552 939 362 1303 2428 3426 Fragments arranged by size: 3426 2428 1303 939 552 362 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8271 Fragments arranged by size: 8271 683 56 BtsI GCAGTG_nn' Cuts at: 3411 4136 5211 5710 6526 7320 7340 3411 Size: 725 1075 499 816 794 20 5081 Fragments arranged by size: 5081 1075 816 794 725 499 20 ClaI AT'CG_AT Cuts at: 1554 3712 1554 Size: 2158 6852 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2889 2924 4254 4289 529 Size: 35 2325 35 1330 35 5250 Fragments arranged by size: 5250 2325 1330 35 35 35 DraI TTT'AAA Cuts at: 3543 5241 6314 3543 Size: 1698 1073 6239 Fragments arranged by size: 6239 1698 1073 DraIII CAC_nnn'GTG Cuts at: 5371 5371 Size: 9010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2051 4324 8017 809 Size: 507 735 2273 3693 1802 Fragments arranged by size: 3693 2273 1802 735 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4042 5508 6457 Size: 54 1425 50 275 18 2212 1466 949 Cuts at: 6457 7346 8975 8 Size: 889 1629 43 Fragments arranged by size: 2212 1629 1466 1425 949 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6457 1537 Size: 275 4645 4090 Fragments arranged by size: 4645 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4581 4668 6940 1353 Size: 3228 87 2272 3423 Fragments arranged by size: 3423 3228 2272 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3237 7597 7971 8117 387 Size: 1064 138 1648 4360 374 146 1280 Fragments arranged by size: 4360 1648 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2894 2894 Size: 9010 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2381 2735 2888 4443 7073 7670 Size: 393 639 110 354 153 1555 2630 597 Cuts at: 7670 8457 8725 1239 Size: 787 268 1524 Fragments arranged by size: 2630 1555 1524 787 639 597 393 354 268 153 110 EcoNI CCTnn'n_nnAGG Cuts at: 2301 2301 Size: 9010 EcoO109I rG'GnC_Cy Cuts at: 3079 4649 3079 Size: 1570 7440 EcoRI G'AATT_C Cuts at: 2520 2818 3045 5360 6274 6319 6697 2520 Size: 298 227 2315 914 45 378 4833 Fragments arranged by size: 4833 2315 914 378 298 227 45 EcoRV GAT'ATC Cuts at: 3487 5545 3487 Size: 2058 6952 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2982 3371 3389 4325 5491 5631 2982 Size: 389 18 936 1166 140 6361 Fragments arranged by size: 6361 1166 936 389 140 18 FspI TGC'GCA Cuts at: 7516 7516 Size: 9010 HaeII r_GCGC'y Cuts at: 1678 3832 8157 1678 Size: 2154 4325 2531 Fragments arranged by size: 4325 2531 2154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2716 2731 2748 2763 4103 Size: 32 135 32 1144 15 17 15 1340 Cuts at: 4103 4135 4464 4496 4667 4699 4955 4987 6148 Size: 32 329 32 171 32 256 32 1161 Cuts at: 6148 6180 7651 7683 7725 7757 1373 Size: 32 1471 32 42 32 2626 Fragments arranged by size: 2626 1471 1340 1161 1144 329 256 171 135 42 32 32 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 3591 4168 4317 5036 5670 5783 Size: 988 281 1653 577 149 719 634 113 Cuts at: 5783 6354 6600 669 Size: 571 246 3079 Fragments arranged by size: 3079 1653 988 719 634 577 571 281 246 149 113 HindIII A'AGCT_T Cuts at: 748 2264 748 Size: 1516 7494 HpaI GTT'AAC Cuts at: 4168 6600 4168 Size: 2432 6578 KasI G'GCGC_C Cuts at: 3828 3828 Size: 9010 KpnI G_GTAC'C Cuts at: 6335 6335 Size: 9010 MfeI C'AATT_G Cuts at: 6022 6609 6022 Size: 587 8423 MluI A'CGCG_T Cuts at: 8886 8886 Size: 9010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2889 4725 8124 8308 1305 Size: 1584 1836 3399 184 2007 Fragments arranged by size: 3399 2007 1836 1584 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4044 10 Size: 54 1425 343 2212 4976 Fragments arranged by size: 4976 2212 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2532 6109 6195 6968 7327 7486 518 Size: 648 1366 3577 86 773 359 159 2042 Fragments arranged by size: 3577 2042 1366 773 648 359 159 86 MspA1I CmG'CkG Cuts at: 1606 3383 3853 3970 4061 5098 6172 6426 7103 Size: 1777 470 117 91 1037 1074 254 677 Cuts at: 7103 8251 8496 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1777 1148 1074 1037 677 470 254 245 117 91 NarI GG'CG_CC Cuts at: 3829 3829 Size: 9010 NcoI C'CATG_G Cuts at: 513 1065 2004 2366 3669 6097 513 Size: 552 939 362 1303 2428 3426 Fragments arranged by size: 3426 2428 1303 939 552 362 NdeI CA'TA_TG Cuts at: 387 2782 3693 4804 387 Size: 2395 911 1111 4593 Fragments arranged by size: 4593 2395 1111 911 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9010 NotI GC'GGCC_GC Cuts at: 6457 6457 Size: 9010 NsiI A_TGCA'T Cuts at: 3688 3688 Size: 9010 NspI r_CATG'y Cuts at: 1132 1229 2448 3021 3743 6183 6368 7913 1132 Size: 97 1219 573 722 2440 185 1545 2229 Fragments arranged by size: 2440 2229 1545 1219 722 573 185 97 PacI TTA_AT'TAA Cuts at: 4446 4446 Size: 9010 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9010 PciI A'CATG_T Cuts at: 7909 7909 Size: 9010 PflMI CCAn_nnn'nTGG Cuts at: 1108 4064 4841 1108 Size: 2956 777 5277 Fragments arranged by size: 5277 2956 777 PfoI T'CCnGG_A Cuts at: 2040 4579 2040 Size: 2539 6471 PmeI GTTT'AAAC Cuts at: 6314 6314 Size: 9010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 7079 7111 8592 8624 4208 Size: 32 2839 32 1481 32 4594 Fragments arranged by size: 4594 2839 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4649 4649 Size: 9010 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9010 PsiI TTA'TAA Cuts at: 3653 4527 6204 6580 3653 Size: 874 1677 376 6083 Fragments arranged by size: 6083 1677 874 376 PstI C_TGCA'G Cuts at: 830 2302 3574 5264 6171 6362 830 Size: 1472 1272 1690 907 191 3478 Fragments arranged by size: 3478 1690 1472 1272 907 191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9010 PvuII CAG'CTG Cuts at: 3383 3853 3970 5098 6172 3383 Size: 470 117 1128 1074 6221 Fragments arranged by size: 6221 1128 1074 470 117 SacI G_AGCT'C Cuts at: 2896 2896 Size: 9010 SalI G'TCGA_C Cuts at: 1936 6352 1936 Size: 4416 4594 SapI GCTCTTCn'nnn_ Cuts at: 4581 4581 Size: 9010 SbfI CC_TGCA'GG Cuts at: 6362 6362 Size: 9010 ScaI AGT'ACT Cuts at: 7258 8970 7258 Size: 1712 7298 SfcI C'TryA_G Cuts at: 826 1045 2298 3570 4770 5007 5260 5805 6167 Size: 219 1253 1272 1200 237 253 545 362 Cuts at: 6167 6358 7493 8174 8365 826 Size: 191 1135 681 191 1471 Fragments arranged by size: 1471 1272 1253 1200 1135 681 545 362 253 237 219 191 191 SfoI GGC'GCC Cuts at: 3830 3830 Size: 9010 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9010 SmaI CCC'GGG Cuts at: 1872 6337 1872 Size: 4465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 4142 4606 5899 7114 8015 Size: 197 926 189 2010 464 1293 1215 901 Cuts at: 8015 8277 820 Size: 262 1553 Fragments arranged by size: 2010 1553 1293 1215 926 901 464 262 197 189 SnaBI TAC'GTA Cuts at: 493 6801 493 Size: 6308 2702 SpeI A'CTAG_T Cuts at: 152 4390 5339 152 Size: 4238 949 3823 Fragments arranged by size: 4238 3823 949 SphI G_CATG'C Cuts at: 1132 3743 6183 6368 1132 Size: 2611 2440 185 3774 Fragments arranged by size: 3774 2611 2440 185 SspI AAT'ATT Cuts at: 5 52 3560 4601 5202 5722 6934 5 Size: 47 3508 1041 601 520 1212 2081 Fragments arranged by size: 3508 2081 1212 1041 601 520 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9010 StyI C'CwwG_G Cuts at: 513 1065 2004 2366 2735 3390 3434 3669 6097 Size: 552 939 362 369 655 44 235 2428 Cuts at: 6097 6208 6218 6449 513 Size: 111 10 231 3074 Fragments arranged by size: 3074 2428 939 655 552 369 362 235 231 111 44 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2249 2913 5223 7037 7054 7207 7392 Size: 1255 669 664 2310 1814 17 153 185 Cuts at: 7392 7837 325 Size: 445 1498 Fragments arranged by size: 2310 1814 1498 1255 669 664 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2017 2584 2798 Size: 274 80 33 51 1443 40 567 214 Cuts at: 2798 4979 7256 8968 96 Size: 2181 2277 1712 138 Fragments arranged by size: 2277 2181 1712 1443 567 274 214 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5076 5108 6078 6110 8970 9002 5076 Size: 32 970 32 2860 32 5084 Fragments arranged by size: 5084 2860 970 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9010 XbaI T'CTAG_A Cuts at: 6346 6346 Size: 9010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9010 XmaI C'CCGG_G Cuts at: 1870 6335 1870 Size: 4465 4545 XmnI GAAnn'nnTTC Cuts at: 2391 4600 5269 5870 7139 2391 Size: 2209 669 601 1269 4262 Fragments arranged by size: 4262 2209 1269 669 601 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6818 276 Size: 53 83 186 936 5284 2468 Fragments arranged by size: 5284 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BsiWI BstZ17I Bsu36I FseI FspAI NaeI NgoMIV NruI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI