Restriction map for
pFN21AA0996
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BfrI BglII BlnI BsaI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI FspI HincII HindII HpaI
KasI KpnI MfeI MluI MluNI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SalI SbfI SfoI SgrAI
SmaI SnaBI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 88 GACGTC GACGT^C
AccI 52 GTCTAC GT^[AC][GT]AC
AccIII 716 TCCGGA T^CCGGA
AcyI 88 GACGTC G[GA]^CG[CT]C
AlwI 1939 GATCC GGATC(4/5)
AlwNI 559 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2446 CCTAAGG CC^T[ACGT]AGG
AseI 1615 ATTAAT AT^TAAT
AsnI 1615 ATTAAT AT^TAAT
AvaI 1127 CTCGAG C^[CT]CG[GA]G
BbvCI 7 GCTGAGG CCTCAGC(-5/-2)
BcgI 1426 GCATTCTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1809 GGATAC GTATCC(6/5)
BclI 1644 TGATCA T^GATCA
BglI 121 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 213 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 213 GCTGAGC GC^T[ACGT]AGC
BsaAI 1628 TACGTG [CT]AC^GT[GA]
BsaBI 2256 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 88 GACGTC G[GA]^CG[CT]C
BsaWI 716 TCCGGA [AT]^CCGG[AT]
BseAI 716 TCCGGA T^CCGGA
BsoBI 1127 CTCGAG C^[CT]CG[GA]G
BspEI 716 TCCGGA T^CCGGA
BspHI 278 TCATGA T^CATGA
BspMI 2388 ACCTGC ACCTGC(4/8)
BsrDI 1229 GCAATG GCAATG(2/0)
BssSI 1039 CACGAG CACGAG(-5/-1)
BstAPI 778 GCACACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 221 CCATCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1938 AGATCC [GA]^GATC[CT]
Bsu36I 2446 CCTAAGG CC^T[ACGT]AGG
CelII 213 GCTGAGC GC^T[ACGT]AGC
EaeI 98 TGGCCG [CT]^GGCC[GA]
Eco81I 2446 CCTAAGG CC^T[ACGT]AGG
EcoNI 1551 CCTCACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2343 GATATC GAT^ATC
EcoT22I 1516 ATGCAT ATGCA^T
FbaI 1644 TGATCA T^GATCA
HaeII 217 AGCGCC [GA]GCGC^[CT]
Hin1I 88 GACGTC G[GA]^CG[CT]C
MamI 2256 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1938 AGATCC [GA]^GATC[CT]
MroI 716 TCCGGA T^CCGGA
NcoI 125 CCATGG C^CATGG
NsiI 1516 ATGCAT ATGCA^T
PaeR7I 1127 CTCGAG C^TCGAG
PflMI 2230 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1615 ATTAAT AT^TAAT
RcaI 278 TCATGA T^CATGA
SexAI 757 ACCAGGT A^CC[AT]GGT
SfiI 120 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 888 ACTAGT A^CTAGT
SphI 32 GCATGC GCATG^C
StuI 614 AGGCCT AGG^CCT
Van91I 2230 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1127 CTCGAG C^TCGAG
XhoII 1938 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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ApaI 82 GGGCCC GGGCC^C
157 GGGCCC
Asp700I 1952 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2464 GAAGGACTTC
AvaII 339 GGACC G^G[AT]CC
2111 GGACC
BanII 82 GGGCCC G[GA]GC[CT]^C
157 GGGCCC
BcnI 342 CCCGG CC^[GC]GG
2019 CCGGG
BsmAI 1269 GAGAC GTCTC(1/5)
1305 GAGAC
BsmI 1365 GAATGC GAATGC(1/-1)
1426 GCATTC
BsrBI 61 CCGCTC CCGCTC(-3/-3)
439 GAGCGG
BstBI 948 TTCGAA TT^CGAA
1196 TTCGAA
DraI 934 TTTAAA TTT^AAA
1045 TTTAAA
EcoRI 954 GAATTC G^AATTC
1729 GAATTC
HindIII 629 AAGCTT A^AGCTT
1529 AAGCTT
KspI 110 CCGCGG CCGC^GG
184 CCGCGG
NciI 342 CCCGG CC^[GC]GG
2019 CCGGG
NspI 32 GCATGC [GA]CATG^[CT]
48 GCATGT
NspV 948 TTCGAA TT^CGAA
1196 TTCGAA
PleI 1271 GACTC GAGTC(4/5)
2162 GAGTC
PstI 265 CTGCAG CTGCA^G
748 CTGCAG
SacII 110 CCGCGG CCGC^GG
184 CCGCGG
SapI 742 GAAGAGC GCTCTTC(1/4)
1255 GAAGAGC
ScaI 386 AGTACT AGT^ACT
1659 AGTACT
SfaNI 1033 GATGC GCATC(5/9)
2029 GCATC
SfcI 265 CTGCAG C^T[GA][CT]AG
748 CTGCAG
SfuI 948 TTCGAA TT^CGAA
1196 TTCGAA
SmlI 517 CTCAAG C^T[CT][GA]AG
1127 CTCGAG
XmnI 1952 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2464 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I