Restriction map for
pFN21AA0996
(Linear) (Six-base) MAPSORT of: KIAA0996.seq Check: 919 from: 1 to: 2544
KIAA0996.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2397 2544
Size: 2397 147
AatII G_ACGT'C
Cuts at: 0 92 2544
Size: 92 2452
AccI GT'mk_AC
Cuts at: 0 53 2544
Size: 53 2491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 686 1766 2519 2544
Size: 373 313 1080 753 25
Fragments arranged by size:
1080 753 373 313 25
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 499 2544
Size: 499 2045
AlwNI CAG_nnn'CTG
Cuts at: 0 564 2544
Size: 564 1980
ApaI G_GGCC'C
Cuts at: 0 86 161 2544
Size: 86 75 2383
Fragments arranged by size:
2383 86 75
ApoI r'AATT_y
Cuts at: 0 672 931 954 1721 1729 2544
Size: 672 259 23 767 8 815
Fragments arranged by size:
815 767 672 259 23 8
AseI AT'TA_AT
Cuts at: 0 1616 2544
Size: 1616 928
AvaI C'yCGr_G
Cuts at: 0 1127 2544
Size: 1127 1417
BanII G_rGCy'C
Cuts at: 0 86 161 2544
Size: 86 75 2383
Fragments arranged by size:
2383 86 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 311 845 1058 1949 2318 2544
Size: 311 534 213 891 369 226
Fragments arranged by size:
891 534 369 311 226 213
BbvCI CC'TCA_GC
Cuts at: 0 8 2544
Size: 8 2536
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1415 1449 2544
Size: 1415 34 1095
Fragments arranged by size:
1415 1095 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1803 2544
Size: 1803 741
BclI T'GATC_A
Cuts at: 0 1644 2544
Size: 1644 900
BfrBI ATG'CAT
Cuts at: 0 1518 2544
Size: 1518 1026
BglI GCCn_nnn'nGGC
Cuts at: 0 127 2544
Size: 127 2417
BlpI GC'TnA_GC
Cuts at: 0 214 2544
Size: 214 2330
Bme1580I G_kGCm'C
Cuts at: 0 86 161 319 2544
Size: 86 75 158 2225
Fragments arranged by size:
2225 158 86 75
BmgBI CAC'GTC
Cuts at: 0 228 255 2544
Size: 228 27 2289
Fragments arranged by size:
2289 228 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 2325 2544
Size: 2325 219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 454 1258 1844 2544
Size: 211 243 804 586 700
Fragments arranged by size:
804 700 586 243 211
Bpu10I CC'TnA_GC
Cuts at: 0 8 451 2544
Size: 8 443 2093
Fragments arranged by size:
2093 443 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 2544
Size: 502 2042
BsaAI yAC'GTr
Cuts at: 0 1630 2544
Size: 1630 914
BsaBI GATnn'nnATC
Cuts at: 0 2260 2544
Size: 2260 284
BsaHI Gr'CG_yC
Cuts at: 0 89 2544
Size: 89 2455
BsaWI w'CCGG_w
Cuts at: 0 716 2544
Size: 716 1828
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1959 1989 2153 2183 2544
Size: 1959 30 164 30 361
Fragments arranged by size:
1959 361 164 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 1536 1777 2005 2008 2544
Size: 451 1085 241 228 3 536
Fragments arranged by size:
1085 536 451 241 228 3
BseYI C'CCAG_C
Cuts at: 0 142 557 1371 2544
Size: 142 415 814 1173
Fragments arranged by size:
1173 814 415 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 409 761 812 1363 1812 2544
Size: 379 30 352 51 551 449 732
Fragments arranged by size:
732 551 449 379 352 51 30
BsmI GAATG_Cn'
Cuts at: 0 1371 1426 2544
Size: 1371 55 1118
Fragments arranged by size:
1371 1118 55
Bsp1286I G_dGCh'C
Cuts at: 0 86 161 319 2544
Size: 86 75 158 2225
Fragments arranged by size:
2225 158 86 75
BspEI T'CCGG_A
Cuts at: 0 716 2544
Size: 716 1828
BspHI T'CATG_A
Cuts at: 0 278 2544
Size: 278 2266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2397 2544
Size: 2397 147
BsrBI CCG'CTC
Cuts at: 0 63 441 2544
Size: 63 378 2103
Fragments arranged by size:
2103 378 63
BsrDI GCAATG_nn'
Cuts at: 0 1236 2544
Size: 1236 1308
BssSI C'ACGA_G
Cuts at: 0 1039 2544
Size: 1039 1505
BstAPI GCAn_nnn'nTGC
Cuts at: 0 784 2544
Size: 784 1760
BstBI TT'CG_AA
Cuts at: 0 949 1197 2544
Size: 949 248 1347
Fragments arranged by size:
1347 949 248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 2544
Size: 228 2316
BstYI r'GATC_y
Cuts at: 0 1938 2544
Size: 1938 606
Bsu36I CC'TnA_GG
Cuts at: 0 2447 2544
Size: 2447 97
BtgI C'CryG_G
Cuts at: 0 110 125 184 2544
Size: 110 15 59 2360
Fragments arranged by size:
2360 110 59 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2194 2544
Size: 2194 350
BtsI GCAGTG_nn'
Cuts at: 0 321 456 1800 2544
Size: 321 135 1344 744
Fragments arranged by size:
1344 744 321 135
DraI TTT'AAA
Cuts at: 0 936 1047 2544
Size: 936 111 1497
Fragments arranged by size:
1497 936 111
EaeI y'GGCC_r
Cuts at: 0 98 2544
Size: 98 2446
EarI CTCTTCn'nnn_
Cuts at: 0 518 737 1250 1531 1785 2333 2544
Size: 518 219 513 281 254 548 211
Fragments arranged by size:
548 518 513 281 254 219 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 373 454 686 1258 1766 1844 2519
Size: 211 162 81 232 572 508 78 675
Cuts at: 2519 2544
Size: 25
Fragments arranged by size:
675 572 508 232 211 162 81 78
25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1555 2544
Size: 1555 989
EcoO109I rG'GnC_Cy
Cuts at: 0 82 157 158 2544
Size: 82 75 1 2386
Fragments arranged by size:
2386 82 75 1
EcoRI G'AATT_C
Cuts at: 0 954 1729 2544
Size: 954 775 815
Fragments arranged by size:
954 815 775
EcoRV GAT'ATC
Cuts at: 0 2345 2544
Size: 2345 199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1547 2091 2544
Size: 1547 544 453
Fragments arranged by size:
1547 544 453
HaeII r_GCGC'y
Cuts at: 0 221 2544
Size: 221 2323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1200 1232 1284 1316 1888 1920 2311 2343
Size: 1200 32 52 32 572 32 391 32
Cuts at: 2343 2544
Size: 201
Fragments arranged by size:
1200 572 391 201 52 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 629 1529 2544
Size: 629 900 1015
Fragments arranged by size:
1015 900 629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 2544
Size: 1037 1507
MslI CAynn'nnrTG
Cuts at: 0 226 605 788 2544
Size: 226 379 183 1756
Fragments arranged by size:
1756 379 226 183
MspA1I CmG'CkG
Cuts at: 0 16 71 112 186 423 564 1751 2544
Size: 16 55 41 74 237 141 1187 793
Fragments arranged by size:
1187 793 237 141 74 55 41 16
NcoI C'CATG_G
Cuts at: 0 125 2544
Size: 125 2419
NsiI A_TGCA'T
Cuts at: 0 1520 2544
Size: 1520 1024
NspI r_CATG'y
Cuts at: 0 36 52 2544
Size: 36 16 2492
Fragments arranged by size:
2492 36 16
PflMI CCAn_nnn'nTGG
Cuts at: 0 2236 2544
Size: 2236 308
PfoI T'CCnGG_A
Cuts at: 0 1274 2544
Size: 1274 1270
PsiI TTA'TAA
Cuts at: 0 1297 2544
Size: 1297 1247
PspOMI G'GGCC_C
Cuts at: 0 82 157 2544
Size: 82 75 2387
Fragments arranged by size:
2387 82 75
PspXI vC'TCGA_Gb
Cuts at: 0 1127 2544
Size: 1127 1417
PstI C_TGCA'G
Cuts at: 0 269 752 2544
Size: 269 483 1792
Fragments arranged by size:
1792 483 269
PvuII CAG'CTG
Cuts at: 0 423 564 1751 2544
Size: 423 141 1187 793
Fragments arranged by size:
1187 793 423 141
SacII CC_GC'GG
Cuts at: 0 113 187 2544
Size: 113 74 2357
Fragments arranged by size:
2357 113 74
SapI GCTCTTCn'nnn_
Cuts at: 0 737 1250 2544
Size: 737 513 1294
Fragments arranged by size:
1294 737 513
ScaI AGT'ACT
Cuts at: 0 388 1661 2544
Size: 388 1273 883
Fragments arranged by size:
1273 883 388
SexAI A'CCwGG_T
Cuts at: 0 757 2544
Size: 757 1787
SfcI C'TryA_G
Cuts at: 0 265 748 2544
Size: 265 483 1796
Fragments arranged by size:
1796 483 265
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 127 2544
Size: 127 2417
SmlI C'TyrA_G
Cuts at: 0 517 1127 2544
Size: 517 610 1417
Fragments arranged by size:
1417 610 517
SpeI A'CTAG_T
Cuts at: 0 888 2544
Size: 888 1656
SphI G_CATG'C
Cuts at: 0 36 2544
Size: 36 2508
StuI AGG'CCT
Cuts at: 0 616 2544
Size: 616 1928
StyI C'CwwG_G
Cuts at: 0 125 806 1607 2544
Size: 125 681 801 937
Fragments arranged by size:
937 801 681 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 571 2128 2544
Size: 571 1557 416
Fragments arranged by size:
1557 571 416
TatI w'GTAC_w
Cuts at: 0 386 1659 2544
Size: 386 1273 885
Fragments arranged by size:
1273 885 386
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 392 424 2544
Size: 392 32 2120
Fragments arranged by size:
2120 392 32
XhoI C'TCGA_G
Cuts at: 0 1127 2544
Size: 1127 1417
XmnI GAAnn'nnTTC
Cuts at: 0 1956 2468 2544
Size: 1956 512 76
Fragments arranged by size:
1956 512 76
ZraI GAC'GTC
Cuts at: 0 90 2544
Size: 90 2454
Enzymes that do cut:
AarI AatII AccI AcuI AlfI AlwNI ApaI ApoI
AseI AvaI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HindIII MmeI MslI MspA1I
NcoI NsiI NspI PflMI PfoI PsiI PspOMI PspXI
PstI PvuII SacII SapI ScaI SexAI SfcI SfiI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI AscI AsiSI AvrII BaeI BamHI BanI
BbeI BglII BmtI BplI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsrFI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsrI PvuI
RsrII SacI SalI SanDI SbfI SfoI SgfI SgrAI
SmaI SnaBI SrfI SspI SwaI Tth111I XbaI XcmI
XmaI