(Circular) (Six-base) MAPSORT of: pFN21AA0953.seq Check: 9015 from: 1 to: 6889 pFN21AA0953 6889 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4096 4096 Size: 6889 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4699 278 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3762 4232 1937 Size: 1825 470 4594 Fragments arranged by size: 4594 1825 470 Acc65I G'GTAC_C Cuts at: 4210 4210 Size: 6889 AclI AA'CG_TT Cuts at: 1563 5016 5389 1563 Size: 3453 373 3063 Fragments arranged by size: 3453 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3533 4952 6336 6604 1632 Size: 1901 1419 1384 268 1917 Fragments arranged by size: 1917 1901 1419 1384 268 AfeI AGC'GCT Cuts at: 2917 3185 2917 Size: 268 6621 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6692 AflIII A'CryG_T Cuts at: 1203 2220 5788 6765 1203 Size: 1017 3568 977 1327 Fragments arranged by size: 3568 1327 1017 977 AgeI A'CCGG_T Cuts at: 5720 5720 Size: 6889 AhdI GACnn_n'nnGTC Cuts at: 2401 5618 2401 Size: 3217 3672 AleI CACnn'nnGTG Cuts at: 1166 3827 1166 Size: 2661 4228 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6889 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3107 3139 3107 Size: 32 6857 AlwNI CAG_nnn'CTG Cuts at: 2887 3833 4632 6204 2887 Size: 946 799 1572 3572 Fragments arranged by size: 3572 1572 946 799 ApaI G_GGCC'C Cuts at: 2994 2994 Size: 6889 ApaLI G'TGCA_C Cuts at: 4946 6102 4946 Size: 1156 5733 ApoI r'AATT_y Cuts at: 1493 2490 3411 4198 4424 4576 6685 1493 Size: 997 921 787 226 152 2109 1697 Fragments arranged by size: 2109 1697 997 921 787 226 152 AseI AT'TA_AT Cuts at: 160 1032 5443 160 Size: 872 4411 1606 Fragments arranged by size: 4411 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 AvaI C'yCGr_G Cuts at: 1870 1943 2084 2458 3899 4214 1870 Size: 73 141 374 1441 315 4545 Fragments arranged by size: 4545 1441 374 315 141 73 BamHI G'GATC_C Cuts at: 2080 4219 4586 2080 Size: 2139 367 4383 Fragments arranged by size: 4383 2139 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2165 2357 3089 4210 Size: 325 217 627 243 135 192 732 1121 Cuts at: 4210 5665 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1121 732 627 325 243 217 192 135 BanII G_rGCy'C Cuts at: 1391 2687 2994 3155 3963 4208 1391 Size: 1296 307 161 808 245 4072 Fragments arranged by size: 4072 1296 808 307 245 161 BbeI G_GCGC'C Cuts at: 2169 2169 Size: 6889 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2108 2540 2702 3544 928 Size: 971 209 432 162 842 4273 Fragments arranged by size: 4273 971 842 432 209 162 BbvCI CC'TCA_GC Cuts at: 3023 4044 3023 Size: 1021 5868 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5080 5114 1903 Size: 34 3143 34 3678 Fragments arranged by size: 3678 3143 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3328 4781 5991 3328 Size: 1453 1210 4226 Fragments arranged by size: 4226 1453 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6889 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5500 136 Size: 107 122 71 631 605 3828 1525 Fragments arranged by size: 3828 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6884 6884 Size: 6889 BlpI GC'TnA_GC Cuts at: 3038 4306 3038 Size: 1268 5621 Bme1580I G_kGCm'C Cuts at: 1790 2035 2994 3778 4950 6106 1790 Size: 245 959 784 1172 1156 2573 Fragments arranged by size: 2573 1172 1156 959 784 245 BmgBI CAC'GTC Cuts at: 1318 2197 3730 1318 Size: 879 1533 4477 Fragments arranged by size: 4477 1533 879 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4620 5578 442 Size: 416 53 83 399 24 3203 958 1753 Fragments arranged by size: 3203 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6889 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2661 4114 2661 Size: 1453 5436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 2345 3023 5549 1239 Size: 888 218 678 2526 2579 Fragments arranged by size: 2579 2526 888 678 218 Bpu10I CC'TnA_GC Cuts at: 3023 3596 4044 3023 Size: 573 448 5868 Fragments arranged by size: 5868 573 448 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2288 5014 5879 6177 2288 Size: 2726 865 298 3000 Fragments arranged by size: 3000 2726 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2649 3860 4085 5552 882 Size: 617 1150 1211 225 1467 2219 Fragments arranged by size: 2219 1467 1211 1150 617 225 BsaAI yAC'GTr Cuts at: 493 1204 2221 4680 493 Size: 711 1017 2459 2702 Fragments arranged by size: 2702 2459 1017 711 BsaBI GATnn'nnATC Cuts at: 3437 4059 6883 3437 Size: 622 2824 3443 Fragments arranged by size: 3443 2824 622 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2901 4696 5078 Size: 53 83 186 936 633 735 1795 382 Cuts at: 5078 5713 275 Size: 635 1451 Fragments arranged by size: 1795 1451 936 735 635 633 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2412 4132 5322 5720 5994 6141 6552 2412 Size: 1720 1190 398 274 147 411 2749 Fragments arranged by size: 2749 1720 1190 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3107 3137 3170 3200 1178 Size: 30 165 30 1704 30 33 30 4867 Fragments arranged by size: 4867 1704 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 2642 3128 3698 3761 3953 4090 4093 Size: 1219 237 486 570 63 192 137 3 Cuts at: 4093 4157 1186 Size: 64 3918 Fragments arranged by size: 3918 1219 570 486 237 192 137 64 63 3 BseYI C'CCAG_C Cuts at: 2722 3570 6092 6588 2722 Size: 848 2522 496 3023 Fragments arranged by size: 3023 2522 848 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3218 3641 1163 Size: 573 1482 423 4411 Fragments arranged by size: 4411 1482 573 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4339 5100 6128 1540 Size: 275 186 2338 761 1028 2301 Fragments arranged by size: 2338 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3111 3536 3963 4950 5035 6106 3111 Size: 425 427 987 85 1071 3894 Fragments arranged by size: 3894 1071 987 427 425 85 BsmI GAATG_Cn' Cuts at: 4400 4493 4400 Size: 93 6796 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2687 2994 3111 3155 3536 3778 Size: 399 245 652 307 117 44 381 242 Cuts at: 3778 3963 4208 4950 5035 6106 1391 Size: 185 245 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 652 399 381 307 245 245 242 185 117 85 44 BspEI T'CCGG_A Cuts at: 4132 6552 4132 Size: 2420 4469 BspHI T'CATG_A Cuts at: 3207 3555 4778 6508 3207 Size: 348 1223 1730 3588 Fragments arranged by size: 3588 1730 1223 348 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 3304 4096 4244 844 Size: 1508 952 792 148 3489 Fragments arranged by size: 3489 1508 952 792 148 BsrBI CCG'CTC Cuts at: 1394 1823 4776 1394 Size: 429 2953 3507 Fragments arranged by size: 3507 2953 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2226 5384 5558 65 Size: 1183 978 3158 174 1396 Fragments arranged by size: 3158 1396 1183 978 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2640 2978 4020 5533 5720 1700 Size: 110 830 338 1042 1513 187 2869 Fragments arranged by size: 2869 1513 1042 830 338 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6889 BssHII G'CGCG_C Cuts at: 1925 2929 1925 Size: 1004 5885 BssSI C'ACGA_G Cuts at: 3110 4949 5961 3110 Size: 1839 1012 4038 Fragments arranged by size: 4038 1839 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6889 BstBI TT'CG_AA Cuts at: 2494 5693 2494 Size: 3199 3690 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6889 BstXI CCAn_nnnn'nTGG Cuts at: 2213 2323 3001 4168 2213 Size: 110 678 1167 4934 Fragments arranged by size: 4934 1167 678 110 BstYI r'GATC_y Cuts at: 1972 2080 2349 4219 4586 4971 4988 6440 6526 Size: 108 269 1870 367 385 17 1452 86 Cuts at: 6526 6538 6884 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1870 1452 385 367 346 269 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2076 2076 Size: 6889 BtgI C'CryG_G Cuts at: 513 1065 2688 2961 3147 3221 513 Size: 552 1623 273 186 74 4181 Fragments arranged by size: 4181 1623 552 273 186 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2953 487 Size: 683 56 1727 4423 Fragments arranged by size: 4423 1727 683 56 BtsI GCAGTG_nn' Cuts at: 2266 2600 4009 4405 5199 5219 2266 Size: 334 1409 396 794 20 3936 Fragments arranged by size: 3936 1409 794 396 334 20 ClaI AT'CG_AT Cuts at: 1554 2209 1554 Size: 655 6234 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6854 DraI TTT'AAA Cuts at: 2830 4193 2830 Size: 1363 5526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3077 3506 5896 809 Size: 507 1761 429 2390 1802 Fragments arranged by size: 2390 1802 1761 507 429 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 3224 4336 Size: 54 1425 50 275 18 483 911 1112 Cuts at: 4336 5225 6854 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1112 911 889 483 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4336 1537 Size: 275 2524 4090 Fragments arranged by size: 4090 2524 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 3206 3971 4819 1353 Size: 938 915 765 848 3423 Fragments arranged by size: 3423 938 915 848 765 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3959 5476 5850 5996 387 Size: 1064 138 2370 1517 374 146 1280 Fragments arranged by size: 2370 1517 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3961 3961 Size: 6889 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2345 3023 3533 4952 5549 6336 Size: 393 495 218 678 510 1419 597 787 Cuts at: 6336 6604 1239 Size: 268 1524 Fragments arranged by size: 1524 1419 787 678 597 510 495 393 268 218 EcoNI CCTnn'n_nnAGG Cuts at: 3236 3311 3398 3236 Size: 75 87 6727 Fragments arranged by size: 6727 87 75 EcoO109I rG'GnC_Cy Cuts at: 2569 2990 3217 2569 Size: 421 227 6241 Fragments arranged by size: 6241 421 227 EcoRI G'AATT_C Cuts at: 4198 4576 4198 Size: 378 6511 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2140 2140 Size: 6889 FspI TGC'GCA Cuts at: 5395 5395 Size: 6889 HaeII r_GCGC'y Cuts at: 1678 2169 2919 3187 3641 6036 1678 Size: 491 750 268 454 2395 2531 Fragments arranged by size: 2531 2395 750 491 454 268 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2387 2419 2627 2659 3518 Size: 32 135 32 815 32 208 32 859 Cuts at: 3518 3550 4052 4080 4084 4103 4112 4114 4135 Size: 32 502 28 4 19 9 2 21 Cuts at: 4135 4146 5530 5562 5604 5636 1373 Size: 11 1384 32 42 32 2626 Fragments arranged by size: 2626 1384 859 815 502 208 135 42 32 32 32 32 32 32 32 28 21 19 11 9 4 2 HincII GTy'rAC Cuts at: 669 1657 1938 2062 4233 4479 669 Size: 988 281 124 2171 246 3079 Fragments arranged by size: 3079 2171 988 281 246 124 HindIII A'AGCT_T Cuts at: 748 748 Size: 6889 HpaI GTT'AAC Cuts at: 4479 4479 Size: 6889 KasI G'GCGC_C Cuts at: 2165 2165 Size: 6889 KpnI G_GTAC'C Cuts at: 4214 4214 Size: 6889 MfeI C'AATT_G Cuts at: 4488 4488 Size: 6889 MluI A'CGCG_T Cuts at: 6765 6765 Size: 6889 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2934 6003 6187 1305 Size: 1629 3069 184 2007 Fragments arranged by size: 3069 2007 1629 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 10 Size: 54 1425 343 483 4584 Fragments arranged by size: 4584 1425 483 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2211 2231 3827 3852 4847 5206 5365 Size: 648 1045 20 1596 25 995 359 159 Cuts at: 5365 518 Size: 2042 Fragments arranged by size: 2042 1596 1045 995 648 359 159 25 20 MspA1I CmG'CkG Cuts at: 1606 2251 2690 2963 3031 3223 3771 4048 4305 Size: 645 439 273 68 192 548 277 257 Cuts at: 4305 4982 6130 6375 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 645 548 439 277 273 257 245 192 68 NarI GG'CG_CC Cuts at: 2166 2166 Size: 6889 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6337 NdeI CA'TA_TG Cuts at: 387 387 Size: 6889 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6889 NotI GC'GGCC_GC Cuts at: 4336 4336 Size: 6889 NspI r_CATG'y Cuts at: 1132 1229 2624 3124 3895 4247 5792 1132 Size: 97 1395 500 771 352 1545 2229 Fragments arranged by size: 2229 1545 1395 771 500 352 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6889 PciI A'CATG_T Cuts at: 5788 5788 Size: 6889 PflMI CCAn_nnn'nTGG Cuts at: 1108 2565 3244 4186 1108 Size: 1457 679 942 3811 Fragments arranged by size: 3811 1457 942 679 PfoI T'CCnGG_A Cuts at: 3126 3369 3126 Size: 243 6646 PmeI GTTT'AAAC Cuts at: 4193 4193 Size: 6889 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3107 3139 4958 4990 6471 6503 3107 Size: 32 1819 32 1481 32 3493 Fragments arranged by size: 3493 1819 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2569 3217 2569 Size: 648 6241 PshAI GACnn'nnGTC Cuts at: 1613 2199 1613 Size: 586 6303 PsiI TTA'TAA Cuts at: 4459 4459 Size: 6889 PspOMI G'GGCC_C Cuts at: 2990 2990 Size: 6889 PspXI vC'TCGA_Gb Cuts at: 2458 2458 Size: 6889 PstI C_TGCA'G Cuts at: 830 2349 2556 3315 4107 4241 830 Size: 1519 207 759 792 134 3478 Fragments arranged by size: 3478 1519 792 759 207 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6889 PvuII CAG'CTG Cuts at: 2251 3031 4048 2251 Size: 780 1017 5092 Fragments arranged by size: 5092 1017 780 RsrII CG'GwC_CG Cuts at: 4018 4018 Size: 6889 SacI G_AGCT'C Cuts at: 3963 3963 Size: 6889 SacII CC_GC'GG Cuts at: 2691 2964 3224 2691 Size: 273 260 6356 Fragments arranged by size: 6356 273 260 SalI G'TCGA_C Cuts at: 1936 4231 1936 Size: 2295 4594 SanDI GG'GwC_CC Cuts at: 2569 2569 Size: 6889 SbfI CC_TGCA'GG Cuts at: 3315 4107 4241 3315 Size: 792 134 5963 Fragments arranged by size: 5963 792 134 ScaI AGT'ACT Cuts at: 3000 5137 6849 3000 Size: 2137 1712 3040 Fragments arranged by size: 3040 2137 1712 SexAI A'CCwGG_T Cuts at: 2868 2868 Size: 6889 SfcI C'TryA_G Cuts at: 826 1045 2345 2552 2615 3311 3580 4103 4237 Size: 219 1300 207 63 696 269 523 134 Cuts at: 4237 5372 6053 6244 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1300 1135 696 681 523 269 219 207 191 134 63 SfoI GGC'GCC Cuts at: 2167 2167 Size: 6889 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6889 SmaI CCC'GGG Cuts at: 1872 4216 1872 Size: 2344 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 2458 4993 5894 6156 820 Size: 197 926 360 155 2535 901 262 1553 Fragments arranged by size: 2535 1553 926 901 360 262 197 155 SnaBI TAC'GTA Cuts at: 493 4680 493 Size: 4187 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6889 SphI G_CATG'C Cuts at: 1132 4247 1132 Size: 3115 3774 SspI AAT'ATT Cuts at: 5 52 4813 5 Size: 47 4761 2081 Fragments arranged by size: 4761 2081 47 StuI AGG'CCT Cuts at: 1517 2514 1517 Size: 997 5892 StyI C'CwwG_G Cuts at: 513 1065 2805 4328 513 Size: 552 1740 1523 3074 Fragments arranged by size: 3074 1740 1523 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4916 4933 5086 5271 5716 325 Size: 1255 3336 17 153 185 445 1498 Fragments arranged by size: 3336 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 2998 5135 Size: 274 80 33 51 1443 864 157 2137 Cuts at: 5135 6847 96 Size: 1712 138 Fragments arranged by size: 2137 1712 1443 864 274 157 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6849 6881 6849 Size: 32 6857 Tth111I GACn'n_nGTC Cuts at: 1316 3548 1316 Size: 2232 4657 XbaI T'CTAG_A Cuts at: 3427 4225 3427 Size: 798 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2983 3394 2983 Size: 411 6478 XhoI C'TCGA_G Cuts at: 1943 2458 1943 Size: 515 6374 XmaI C'CCGG_G Cuts at: 1870 4214 1870 Size: 2344 4545 XmnI GAAnn'nnTTC Cuts at: 5018 5018 Size: 6889 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4697 276 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI SapI SfiI SrfI SwaI