(Circular) (Six-base) MAPSORT of: pFN21AA0949.seq Check: 8701 from: 1 to: 9340 pFN21AA0949 9340 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4799 5171 5240 5580 5890 5920 4799 Size: 372 69 340 310 30 8219 Fragments arranged by size: 8219 372 340 310 69 30 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7150 278 Size: 53 83 186 936 5614 2468 Fragments arranged by size: 5614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6683 1937 Size: 4746 4594 Acc65I G'GTAC_C Cuts at: 6661 6661 Size: 9340 AclI AA'CG_TT Cuts at: 1563 7467 7840 1563 Size: 5904 373 3063 Fragments arranged by size: 5904 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2415 2484 2676 2982 3381 3671 3962 Size: 473 310 69 192 306 399 290 291 Cuts at: 3962 4187 4364 4382 5156 5344 6618 7403 8787 Size: 225 177 18 774 188 1274 785 1384 Cuts at: 8787 9055 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1274 785 774 473 399 310 306 291 290 268 225 192 188 177 69 18 AfeI AGC'GCT Cuts at: 5219 5219 Size: 9340 AflII C'TTAA_G Cuts at: 820 1017 4619 820 Size: 197 3602 5541 Fragments arranged by size: 5541 3602 197 AflIII A'CryG_T Cuts at: 1203 8239 9216 1203 Size: 7036 977 1327 Fragments arranged by size: 7036 1327 977 AgeI A'CCGG_T Cuts at: 8171 8171 Size: 9340 AhdI GACnn_n'nnGTC Cuts at: 2421 8069 2421 Size: 5648 3692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9340 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2142 4907 1184 Size: 958 2765 5617 Fragments arranged by size: 5617 2765 958 AlwNI CAG_nnn'CTG Cuts at: 3214 3359 3940 4030 5282 5557 7083 8655 3214 Size: 145 581 90 1252 275 1526 1572 3899 Fragments arranged by size: 3899 1572 1526 1252 581 275 145 90 ApaI G_GGCC'C Cuts at: 6188 6302 6188 Size: 114 9226 ApaLI G'TGCA_C Cuts at: 2741 3661 4709 7397 8553 2741 Size: 920 1048 2688 1156 3528 Fragments arranged by size: 3528 2688 1156 1048 920 ApoI r'AATT_y Cuts at: 1493 2399 2517 2880 3053 3410 4604 5036 5463 Size: 906 118 363 173 357 1194 432 427 Cuts at: 5463 5810 5849 5978 6649 6875 7027 9136 1493 Size: 347 39 129 671 226 152 2109 1697 Fragments arranged by size: 2109 1697 1194 906 671 432 427 363 357 347 226 173 152 129 118 39 AseI AT'TA_AT Cuts at: 160 1032 7894 160 Size: 872 6862 1606 Fragments arranged by size: 6862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9340 AvaI C'yCGr_G Cuts at: 1870 1943 4428 5171 5843 6147 6539 6665 1870 Size: 73 2485 743 672 304 392 126 4545 Fragments arranged by size: 4545 2485 743 672 392 304 126 73 BamHI G'GATC_C Cuts at: 6670 7037 6670 Size: 367 8973 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3542 4915 5078 5384 5878 Size: 325 217 627 1755 1373 163 306 494 Cuts at: 5878 6661 8116 618 Size: 783 1455 1842 Fragments arranged by size: 1842 1755 1455 1373 783 627 494 325 306 217 163 BanII G_rGCy'C Cuts at: 1391 2529 2694 3222 4050 4419 4881 5400 5661 Size: 1138 165 528 828 369 462 519 261 Cuts at: 5661 5760 6188 6302 6659 1391 Size: 99 428 114 357 4072 Fragments arranged by size: 4072 1138 828 528 519 462 428 369 357 261 165 114 99 BbeI G_GCGC'C Cuts at: 3546 3546 Size: 9340 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2350 3335 3659 4175 4409 4581 5449 Size: 971 451 985 324 516 234 172 868 Cuts at: 5449 6013 6561 6624 928 Size: 564 548 63 3644 Fragments arranged by size: 3644 985 971 868 564 548 516 451 324 234 172 63 BbvCI CC'TCA_GC Cuts at: 2685 5717 6130 2685 Size: 3032 413 5895 Fragments arranged by size: 5895 3032 413 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7531 7565 1903 Size: 34 5594 34 3678 Fragments arranged by size: 5594 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4696 5780 5801 7232 8442 4696 Size: 1084 21 1431 1210 5594 Fragments arranged by size: 5594 1431 1210 1084 21 BclI T'GATC_A Cuts at: 1548 2904 1548 Size: 1356 7984 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3287 7951 136 Size: 107 122 71 631 605 1615 4664 1525 Fragments arranged by size: 4664 1615 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9335 9335 Size: 9340 BlpI GC'TnA_GC Cuts at: 2502 6515 6757 2502 Size: 4013 242 5085 Fragments arranged by size: 5085 4013 242 Bme1580I G_kGCm'C Cuts at: 1790 2745 3665 4713 4920 5387 5521 6188 6302 Size: 955 920 1048 207 467 134 667 114 Cuts at: 6302 7401 8557 1790 Size: 1099 1156 2573 Fragments arranged by size: 2573 1156 1099 1048 955 920 667 467 207 134 114 BmgBI CAC'GTC Cuts at: 1318 5536 1318 Size: 4218 5122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3766 4536 5557 Size: 416 53 83 399 24 2349 770 1021 Cuts at: 5557 7071 8029 442 Size: 1514 958 1753 Fragments arranged by size: 2349 1753 1514 1021 958 770 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5195 1056 Size: 4139 5201 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2397 3973 2397 Size: 1576 7764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2507 2561 2645 2852 3083 3125 3137 Size: 896 372 54 84 207 231 42 12 Cuts at: 3137 3188 3236 3326 3473 3692 3763 3998 4052 Size: 51 48 90 147 219 71 235 54 Cuts at: 4052 4376 4581 4850 4991 5504 6358 6506 8000 Size: 324 205 269 141 513 854 148 1494 Cuts at: 8000 1239 Size: 2579 Fragments arranged by size: 2579 1494 896 854 513 372 324 269 235 231 219 207 205 148 147 141 90 84 71 54 54 51 48 42 12 Bpu10I CC'TnA_GC Cuts at: 2445 2685 5717 6130 2445 Size: 240 3032 413 5655 Fragments arranged by size: 5655 3032 413 240 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2268 3206 4113 6014 7465 8330 8628 2268 Size: 938 907 1901 1451 865 298 2980 Fragments arranged by size: 2980 1901 1451 938 907 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3338 4868 5741 8003 882 Size: 617 1839 1530 873 2262 2219 Fragments arranged by size: 2262 2219 1839 1530 873 617 BsaAI yAC'GTr Cuts at: 493 1204 3660 4776 6342 7131 493 Size: 711 2456 1116 1566 789 2702 Fragments arranged by size: 2702 2456 1566 1116 789 711 BsaBI GATnn'nnATC Cuts at: 9334 9334 Size: 9340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3543 4615 4720 6218 Size: 53 83 186 936 2010 1072 105 1498 Cuts at: 6218 7147 7529 8164 275 Size: 929 382 635 1451 Fragments arranged by size: 2010 1498 1451 1072 936 929 635 382 186 105 83 53 BsaWI w'CCGG_w Cuts at: 4419 5733 7773 8171 8445 8592 9003 4419 Size: 1314 2040 398 274 147 411 4756 Fragments arranged by size: 4756 2040 1314 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3251 3281 5976 6006 1178 Size: 30 165 30 1848 30 2695 30 4512 Fragments arranged by size: 4512 2695 1848 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 2870 2873 3122 3218 3380 3470 3760 Size: 1516 168 3 249 96 162 90 290 Cuts at: 3760 5969 6118 1186 Size: 2209 149 4408 Fragments arranged by size: 4408 2209 1516 290 249 168 162 149 96 90 3 BseYI C'CCAG_C Cuts at: 2727 3074 4880 4947 5198 5220 5580 5681 6379 Size: 347 1806 67 251 22 360 101 698 Cuts at: 6379 6398 8543 9039 2727 Size: 19 2145 496 3028 Fragments arranged by size: 3028 2145 1806 698 496 360 347 251 101 67 22 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3276 4398 5332 5691 5948 1163 Size: 573 1540 1122 934 359 257 4555 Fragments arranged by size: 4555 1540 1122 934 573 359 257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4526 6508 6790 7551 8579 1540 Size: 275 186 2525 1982 282 761 1028 2301 Fragments arranged by size: 2525 2301 1982 1028 761 282 275 186 BsiHKAI G_wGCw'C Cuts at: 2529 2694 2745 3201 3222 3665 3894 4050 4419 Size: 165 51 456 21 443 229 156 369 Cuts at: 4419 4536 4713 4772 6354 7401 7486 8557 2529 Size: 117 177 59 1582 1047 85 1071 3312 Fragments arranged by size: 3312 1582 1071 1047 456 443 369 229 177 165 156 117 85 59 51 21 BsmI GAATG_Cn' Cuts at: 2150 3810 4012 6851 6944 2150 Size: 1660 202 2839 93 4546 Fragments arranged by size: 4546 2839 1660 202 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2529 2694 2745 3201 3222 3665 3894 Size: 399 739 165 51 456 21 443 229 Cuts at: 3894 4050 4419 4536 4713 4772 4881 4920 5387 Size: 156 369 117 177 59 109 39 467 Cuts at: 5387 5400 5521 5661 5760 6188 6302 6354 6659 Size: 13 121 140 99 428 114 52 305 Cuts at: 6659 7401 7486 8557 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 739 467 456 443 428 399 369 305 229 177 165 156 140 121 117 114 109 99 85 59 52 51 39 21 13 BspEI T'CCGG_A Cuts at: 5733 9003 5733 Size: 3270 6070 BspHI T'CATG_A Cuts at: 4225 5496 7229 8959 4225 Size: 1271 1733 1730 4606 Fragments arranged by size: 4606 1733 1730 1271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4107 4312 4799 5171 5240 5580 5890 5920 Size: 3263 205 487 372 69 340 310 30 Cuts at: 5920 6695 844 Size: 775 3489 Fragments arranged by size: 3489 3263 775 487 372 340 310 205 69 30 BsrBI CCG'CTC Cuts at: 1394 1823 3727 5515 6532 7227 1394 Size: 429 1904 1788 1017 695 3507 Fragments arranged by size: 3507 1904 1788 1017 695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7835 8009 65 Size: 1183 6587 174 1396 Fragments arranged by size: 6587 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5026 6570 7984 8171 1700 Size: 110 3216 1544 1414 187 2869 Fragments arranged by size: 3216 2869 1544 1414 187 110 BsrGI T'GTAC_A Cuts at: 96 3559 96 Size: 3463 5877 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9340 BssSI C'ACGA_G Cuts at: 2852 3200 6266 7400 8412 2852 Size: 348 3066 1134 1012 3780 Fragments arranged by size: 3780 3066 1134 1012 348 BstAPI GCAn_nnn'nTGC Cuts at: 794 3805 3994 5727 794 Size: 3011 189 1733 4407 Fragments arranged by size: 4407 3011 1733 189 BstBI TT'CG_AA Cuts at: 8144 8144 Size: 9340 BstEII G'GTnAC_C Cuts at: 1184 5224 1184 Size: 4040 5300 BstXI CCAn_nnnn'nTGG Cuts at: 5633 5981 6367 5633 Size: 348 386 8606 Fragments arranged by size: 8606 386 348 BstYI r'GATC_y Cuts at: 1972 2105 2326 2930 3369 6114 6670 7037 7422 Size: 133 221 604 439 2745 556 367 385 Cuts at: 7422 7439 8891 8977 8989 9335 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2745 1977 1452 604 556 439 385 367 346 221 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 6199 6199 Size: 9340 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8788 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2915 487 Size: 683 56 1689 6912 Fragments arranged by size: 6912 1689 683 56 BtsI GCAGTG_nn' Cuts at: 2210 3737 3888 6856 7650 7670 2210 Size: 1527 151 2968 794 20 3880 Fragments arranged by size: 3880 2968 1527 794 151 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9305 DraI TTT'AAA Cuts at: 6644 6644 Size: 9340 DraIII CAC_nnn'GTG Cuts at: 4686 4686 Size: 9340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6601 8347 809 Size: 507 5285 1746 1802 Fragments arranged by size: 5285 1802 1746 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6475 6505 6787 Size: 54 1425 50 275 18 4645 30 282 Cuts at: 6787 7676 9305 8 Size: 889 1629 43 Fragments arranged by size: 4645 1629 1425 889 282 275 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 6505 6787 1537 Size: 275 4693 282 4090 Fragments arranged by size: 4693 4090 282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 2691 3018 3336 3357 3426 4062 5505 Size: 963 375 327 318 21 69 636 1443 Cuts at: 5505 5905 7270 1353 Size: 400 1365 3423 Fragments arranged by size: 3423 1443 1365 963 636 400 375 327 318 69 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2582 3578 4414 4501 6460 7927 Size: 1064 138 993 996 836 87 1959 1467 Cuts at: 7927 8301 8447 387 Size: 374 146 1280 Fragments arranged by size: 1959 1467 1280 1064 996 993 836 374 146 138 87 EcoICRI GAG'CTC Cuts at: 2527 2692 3220 4048 4417 2527 Size: 165 528 828 369 7450 Fragments arranged by size: 7450 828 528 369 165 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2135 2415 2484 2507 2561 2645 Size: 393 473 30 280 69 23 54 84 Cuts at: 2645 2676 2852 2982 3083 3125 3137 3188 3236 Size: 31 176 130 101 42 12 51 48 Cuts at: 3236 3326 3381 3473 3671 3692 3763 3962 3998 Size: 90 55 92 198 21 71 199 36 Cuts at: 3998 4052 4187 4364 4376 4382 4581 4850 4991 Size: 54 135 177 12 6 199 269 141 Cuts at: 4991 5156 5344 5504 6358 6506 6618 7403 8000 Size: 165 188 160 854 148 112 785 597 Cuts at: 8000 8787 9055 1239 Size: 787 268 1524 Fragments arranged by size: 1524 854 787 785 597 473 393 280 269 268 199 199 198 188 177 176 165 160 148 141 135 130 112 101 92 90 84 71 69 55 54 54 51 48 42 36 31 30 23 21 12 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 2117 4358 6269 2117 Size: 2241 1911 5188 Fragments arranged by size: 5188 2241 1911 EcoO109I rG'GnC_Cy Cuts at: 2122 3697 5479 6138 6185 6298 6395 6594 2122 Size: 1575 1782 659 47 113 97 199 4868 Fragments arranged by size: 4868 1782 1575 659 199 113 97 47 EcoRI G'AATT_C Cuts at: 2399 5849 6649 7027 2399 Size: 3450 800 378 4712 Fragments arranged by size: 4712 3450 800 378 EcoRV GAT'ATC Cuts at: 2230 2230 Size: 9340 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2345 2345 Size: 9340 FspI TGC'GCA Cuts at: 6458 7846 6458 Size: 1388 7952 HaeII r_GCGC'y Cuts at: 1678 3229 3546 5221 6386 8487 1678 Size: 1551 317 1675 1165 2101 2531 Fragments arranged by size: 2531 2101 1675 1551 1165 317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2296 2318 2328 2350 2407 Size: 32 135 32 724 22 10 22 57 Cuts at: 2407 2439 3251 3283 3384 3416 3631 3663 3752 Size: 32 812 32 101 32 215 32 89 Cuts at: 3752 3784 3826 3858 3895 3927 4406 4438 4968 Size: 32 42 32 37 32 479 32 530 Cuts at: 4968 5000 5131 5163 7981 8013 8055 8087 1373 Size: 32 131 32 2818 32 42 32 2626 Fragments arranged by size: 2818 2626 812 724 530 479 215 135 131 101 89 57 42 42 37 32 32 32 32 32 32 32 32 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 3276 6684 6930 669 Size: 988 281 1338 3408 246 3079 Fragments arranged by size: 3408 3079 1338 988 281 246 HindIII A'AGCT_T Cuts at: 748 4015 748 Size: 3267 6073 HpaI GTT'AAC Cuts at: 6930 6930 Size: 9340 KasI G'GCGC_C Cuts at: 3542 3542 Size: 9340 KpnI G_GTAC'C Cuts at: 6665 6665 Size: 9340 MfeI C'AATT_G Cuts at: 6939 6939 Size: 9340 MluI A'CGCG_T Cuts at: 9216 9216 Size: 9340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 2769 3063 5928 8454 8638 1305 Size: 993 471 294 2865 2526 184 2007 Fragments arranged by size: 2865 2526 2007 993 471 294 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7518 Fragments arranged by size: 7518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3393 4732 7298 7657 7816 518 Size: 648 2227 1339 2566 359 159 2042 Fragments arranged by size: 2566 2227 2042 1339 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3115 3432 3472 3847 4198 5766 6601 6756 Size: 1509 317 40 375 351 1568 835 155 Cuts at: 6756 7433 8581 8826 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1568 1509 1148 835 677 375 351 317 245 155 40 NaeI GCC'GGC Cuts at: 6572 6572 Size: 9340 NarI GG'CG_CC Cuts at: 3543 3543 Size: 9340 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8788 NdeI CA'TA_TG Cuts at: 387 387 Size: 9340 NgoMIV G'CCGG_C Cuts at: 6570 6570 Size: 9340 NheI G'CTAG_C Cuts at: 1052 5191 1052 Size: 4139 5201 NotI GC'GGCC_GC Cuts at: 6787 6787 Size: 9340 NspI r_CATG'y Cuts at: 1132 1229 3992 4633 4675 6698 8243 1132 Size: 97 2763 641 42 2023 1545 2229 Fragments arranged by size: 2763 2229 2023 1545 641 97 42 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9340 PciI A'CATG_T Cuts at: 8239 8239 Size: 9340 PflMI CCAn_nnn'nTGG Cuts at: 1108 3871 5166 5447 5875 1108 Size: 2763 1295 281 428 4573 Fragments arranged by size: 4573 2763 1295 428 281 PfoI T'CCnGG_A Cuts at: 2112 4968 5853 2112 Size: 2856 885 5599 Fragments arranged by size: 5599 2856 885 PmeI GTTT'AAAC Cuts at: 6644 6644 Size: 9340 PmlI CAC'GTG Cuts at: 3660 4776 3660 Size: 1116 8224 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4032 4064 7409 7441 8922 8954 4032 Size: 32 3345 32 1481 32 4418 Fragments arranged by size: 4418 3345 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 5479 6138 6594 2122 Size: 3357 659 456 4868 Fragments arranged by size: 4868 3357 659 456 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9340 PsiI TTA'TAA Cuts at: 6910 6910 Size: 9340 PspOMI G'GGCC_C Cuts at: 6184 6298 6184 Size: 114 9226 PspXI vC'TCGA_Gb Cuts at: 5843 5843 Size: 9340 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6161 6193 6161 Size: 32 9308 PstI C_TGCA'G Cuts at: 830 2217 2586 2637 3258 3288 3801 3918 4104 Size: 1387 369 51 621 30 513 117 186 Cuts at: 4104 4332 5765 6692 830 Size: 228 1433 927 3478 Fragments arranged by size: 3478 1433 1387 927 621 513 369 228 186 117 51 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9340 PvuII CAG'CTG Cuts at: 3115 3432 3472 4198 5766 3115 Size: 317 40 726 1568 6689 Fragments arranged by size: 6689 1568 726 317 40 RsrII CG'GwC_CG Cuts at: 4422 6447 4422 Size: 2025 7315 SacI G_AGCT'C Cuts at: 2529 2694 3222 4050 4419 2529 Size: 165 528 828 369 7450 Fragments arranged by size: 7450 828 528 369 165 SalI G'TCGA_C Cuts at: 1936 6682 1936 Size: 4746 4594 SanDI GG'GwC_CC Cuts at: 6138 6138 Size: 9340 SapI GCTCTTCn'nnn_ Cuts at: 3426 4062 5505 3426 Size: 636 1443 7261 Fragments arranged by size: 7261 1443 636 SbfI CC_TGCA'GG Cuts at: 3258 3288 6692 3258 Size: 30 3404 5906 Fragments arranged by size: 5906 3404 30 ScaI AGT'ACT Cuts at: 5960 7588 9300 5960 Size: 1628 1712 6000 Fragments arranged by size: 6000 1712 1628 SexAI A'CCwGG_T Cuts at: 5367 5367 Size: 9340 SfcI C'TryA_G Cuts at: 826 1045 2213 2582 2633 3242 3254 3284 3797 Size: 219 1168 369 51 609 12 30 513 Cuts at: 3797 3914 4100 4328 5761 6061 6688 7823 8504 Size: 117 186 228 1433 300 627 1135 681 Cuts at: 8504 8695 826 Size: 191 1471 Fragments arranged by size: 1471 1433 1168 1135 681 627 609 513 369 300 228 219 191 186 117 51 30 12 SfoI GGC'GCC Cuts at: 3544 3544 Size: 9340 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9340 SmaI CCC'GGG Cuts at: 1872 4430 5173 6541 6667 1872 Size: 2558 743 1368 126 4545 Fragments arranged by size: 4545 2558 1368 743 126 SmlI C'TyrA_G Cuts at: 820 1017 1943 2283 3221 4128 4619 5843 6029 Size: 197 926 340 938 907 491 1224 186 Cuts at: 6029 7444 8345 8607 820 Size: 1415 901 262 1553 Fragments arranged by size: 1553 1415 1224 938 926 907 901 491 340 262 197 186 SnaBI TAC'GTA Cuts at: 493 7131 493 Size: 6638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9340 SphI G_CATG'C Cuts at: 1132 3992 4633 6698 1132 Size: 2860 641 2065 3774 Fragments arranged by size: 3774 2860 2065 641 SspI AAT'ATT Cuts at: 5 52 4642 7264 5 Size: 47 4590 2622 2081 Fragments arranged by size: 4590 2622 2081 47 StuI AGG'CCT Cuts at: 1517 2090 3740 4832 1517 Size: 573 1650 1092 6025 Fragments arranged by size: 6025 1650 1092 573 StyI C'CwwG_G Cuts at: 513 1065 4872 5874 6779 513 Size: 552 3807 1002 905 3074 Fragments arranged by size: 3807 3074 1002 905 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3491 3746 4479 4485 5611 6352 6464 Size: 1255 1911 255 733 6 1126 741 112 Cuts at: 6464 7367 7384 7537 7722 8167 325 Size: 903 17 153 185 445 1498 Fragments arranged by size: 1911 1498 1255 1126 903 741 733 445 255 185 153 112 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3434 3559 4332 Size: 274 80 33 51 1443 1457 125 773 Cuts at: 4332 4959 5835 5958 7586 9298 96 Size: 627 876 123 1628 1712 138 Fragments arranged by size: 1712 1628 1457 1443 876 773 627 274 138 125 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5976 6008 6252 6284 9300 9332 5976 Size: 32 244 32 3016 32 5984 Fragments arranged by size: 5984 3016 244 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9340 XbaI T'CTAG_A Cuts at: 3193 4147 5335 6676 3193 Size: 954 1188 1341 5857 Fragments arranged by size: 5857 1341 1188 954 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4555 4555 Size: 9340 XhoI C'TCGA_G Cuts at: 1943 5843 1943 Size: 3900 5440 XmaI C'CCGG_G Cuts at: 1870 4428 5171 6539 6665 1870 Size: 2558 743 1368 126 4545 Fragments arranged by size: 4545 2558 1368 743 126 XmnI GAAnn'nnTTC Cuts at: 4206 4395 7469 4206 Size: 189 3074 6077 Fragments arranged by size: 6077 3074 189 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7148 276 Size: 53 83 186 936 5614 2468 Fragments arranged by size: 5614 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I FseI FspAI NruI NsiI PacI SacII SfiI SrfI SwaI