(Circular) (Six-base) MAPSORT of: pFN21AA0946.seq Check: 8969 from: 1 to: 6601 pFN21AA0946 6601 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4411 278 Size: 53 83 186 936 2875 2468 Fragments arranged by size: 2875 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3854 3944 1937 Size: 1917 90 4594 Fragments arranged by size: 4594 1917 90 Acc65I G'GTAC_C Cuts at: 3888 3922 3888 Size: 34 6567 AclI AA'CG_TT Cuts at: 1563 4728 5101 1563 Size: 3165 373 3063 Fragments arranged by size: 3165 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4664 6048 6316 1632 Size: 3032 1384 268 1917 Fragments arranged by size: 3032 1917 1384 268 AfeI AGC'GCT Cuts at: 2787 2787 Size: 6601 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6404 AflIII A'CryG_T Cuts at: 1203 3581 5500 6477 1203 Size: 2378 1919 977 1327 Fragments arranged by size: 2378 1919 1327 977 AgeI A'CCGG_T Cuts at: 3343 5432 3343 Size: 2089 4512 AhdI GACnn_n'nnGTC Cuts at: 2869 3297 5330 2869 Size: 428 2033 4140 Fragments arranged by size: 4140 2033 428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6601 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6601 AlwNI CAG_nnn'CTG Cuts at: 3327 3884 4344 5916 3327 Size: 557 460 1572 4012 Fragments arranged by size: 4012 1572 557 460 ApaLI G'TGCA_C Cuts at: 4658 5814 4658 Size: 1156 5445 ApoI r'AATT_y Cuts at: 1493 2374 2903 3104 3389 3800 3910 4136 4288 Size: 881 529 201 285 411 110 226 152 Cuts at: 4288 6397 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 881 529 411 285 226 201 152 110 AseI AT'TA_AT Cuts at: 160 1032 2459 5155 160 Size: 872 1427 2696 1606 Fragments arranged by size: 2696 1606 1427 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 AvaI C'yCGr_G Cuts at: 1870 1943 3926 1870 Size: 73 1983 4545 Fragments arranged by size: 4545 1983 73 BamHI G'GATC_C Cuts at: 2617 3931 4298 2617 Size: 1314 367 4920 Fragments arranged by size: 4920 1314 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3888 3922 5377 618 Size: 325 217 627 2101 34 1455 1842 Fragments arranged by size: 2101 1842 1455 627 325 217 34 BanII G_rGCy'C Cuts at: 1391 3920 1391 Size: 2529 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5630 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4792 4826 1903 Size: 34 50 34 2771 34 3678 Fragments arranged by size: 3678 2771 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4493 5703 4493 Size: 1210 5391 BclI T'GATC_A Cuts at: 1548 2823 3394 1548 Size: 1275 571 4755 Fragments arranged by size: 4755 1275 571 BfrBI ATG'CAT Cuts at: 3067 3067 Size: 6601 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5212 136 Size: 107 122 71 631 605 3540 1525 Fragments arranged by size: 3540 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6596 6596 Size: 6601 BlpI GC'TnA_GC Cuts at: 4018 4018 Size: 6601 Bme1580I G_kGCm'C Cuts at: 1790 2082 4662 5818 1790 Size: 292 2580 1156 2573 Fragments arranged by size: 2580 2573 1156 292 BmgBI CAC'GTC Cuts at: 1318 3123 1318 Size: 1805 4796 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2401 3523 4332 Size: 416 53 83 399 24 984 1122 809 Cuts at: 4332 5290 442 Size: 958 1753 Fragments arranged by size: 1753 1122 984 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2708 1056 Size: 1652 4949 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2766 5261 1239 Size: 1527 2495 2579 Fragments arranged by size: 2579 2495 1527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4726 5591 5889 4726 Size: 865 298 5438 Fragments arranged by size: 5438 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3524 5264 882 Size: 617 2025 1740 2219 Fragments arranged by size: 2219 2025 1740 617 BsaAI yAC'GTr Cuts at: 493 1204 3582 4392 493 Size: 711 2378 810 2702 Fragments arranged by size: 2702 2378 810 711 BsaBI GATnn'nnATC Cuts at: 6595 6595 Size: 6601 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2579 4408 4790 5425 Size: 53 83 186 936 1046 1829 382 635 Cuts at: 5425 275 Size: 1451 Fragments arranged by size: 1829 1451 1046 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2260 3343 5034 5432 5706 5853 6264 2260 Size: 1083 1691 398 274 147 411 2597 Fragments arranged by size: 2597 1691 1083 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2739 2769 1178 Size: 30 165 30 1336 30 5010 Fragments arranged by size: 5010 1336 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2273 1186 Size: 844 243 5514 Fragments arranged by size: 5514 844 243 BseYI C'CCAG_C Cuts at: 5804 6300 5804 Size: 496 6105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2466 3450 1163 Size: 573 730 984 4314 Fragments arranged by size: 4314 984 730 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4051 4812 5840 1540 Size: 275 186 2050 761 1028 2301 Fragments arranged by size: 2301 2050 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4662 4747 5818 4662 Size: 85 1071 5445 Fragments arranged by size: 5445 1071 85 BsmI GAATG_Cn' Cuts at: 3712 4112 4205 3712 Size: 400 93 6108 Fragments arranged by size: 6108 400 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 3920 4662 4747 5818 1391 Size: 399 292 1838 742 85 1071 2174 Fragments arranged by size: 2174 1838 1071 742 399 292 85 BspEI T'CCGG_A Cuts at: 6264 6264 Size: 6601 BspHI T'CATG_A Cuts at: 4490 6220 4490 Size: 1730 4871 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2231 3956 844 Size: 1387 1725 3489 Fragments arranged by size: 3489 1725 1387 BsrBI CCG'CTC Cuts at: 1394 1823 4488 1394 Size: 429 2665 3507 Fragments arranged by size: 3507 2665 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2384 3074 3400 5096 5270 65 Size: 1183 1136 690 326 1696 174 1396 Fragments arranged by size: 1696 1396 1183 1136 690 326 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3343 5245 5432 1700 Size: 110 1533 1902 187 2869 Fragments arranged by size: 2869 1902 1533 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6601 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6601 BssSI C'ACGA_G Cuts at: 4661 5673 4661 Size: 1012 5589 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6601 BstBI TT'CG_AA Cuts at: 2336 5405 2336 Size: 3069 3532 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6601 BstXI CCAn_nnnn'nTGG Cuts at: 2132 2391 2132 Size: 259 6342 BstYI r'GATC_y Cuts at: 1972 2449 2617 3229 3881 3931 4298 4683 4700 Size: 477 168 612 652 50 367 385 17 Cuts at: 4700 6152 6238 6250 6596 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 652 612 477 385 367 346 168 86 50 17 12 BstZ17I GTA'TAC Cuts at: 3855 3855 Size: 6601 BtgI C'CryG_G Cuts at: 513 1065 2125 2418 3407 3766 513 Size: 552 1060 293 989 359 3348 Fragments arranged by size: 3348 1060 989 552 359 293 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5862 Fragments arranged by size: 5862 683 56 BtsI GCAGTG_nn' Cuts at: 2682 2706 4117 4911 4931 2682 Size: 24 1411 794 20 4352 Fragments arranged by size: 4352 1411 794 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6601 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3343 3378 529 Size: 35 2779 35 3752 Fragments arranged by size: 3752 2779 35 35 DraI TTT'AAA Cuts at: 3730 3905 3730 Size: 175 6426 DraIII CAC_nnn'GTG Cuts at: 2612 2612 Size: 6601 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5608 809 Size: 507 4292 1802 Fragments arranged by size: 4292 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4048 4937 6566 Size: 54 1425 50 275 18 2218 889 1629 Cuts at: 6566 8 Size: 43 Fragments arranged by size: 2218 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4048 1537 Size: 275 2236 4090 Fragments arranged by size: 4090 2236 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3249 4531 1353 Size: 1896 1282 3423 Fragments arranged by size: 3423 1896 1282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 5188 5562 5708 387 Size: 1064 138 465 3134 374 146 1280 Fragments arranged by size: 3134 1280 1064 465 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2766 4664 5261 6048 6316 1239 Size: 393 1134 1898 597 787 268 1524 Fragments arranged by size: 1898 1524 1134 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2368 2368 Size: 6601 EcoRI G'AATT_C Cuts at: 2374 3910 4288 2374 Size: 1536 378 4687 Fragments arranged by size: 4687 1536 378 EcoRV GAT'ATC Cuts at: 3672 3672 Size: 6601 FspI TGC'GCA Cuts at: 5107 5107 Size: 6601 HaeII r_GCGC'y Cuts at: 1678 2789 5748 1678 Size: 1111 2959 2531 Fragments arranged by size: 2959 2531 1111 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2855 2887 3283 3315 3860 Size: 32 135 32 1283 32 396 32 545 Cuts at: 3860 3892 5242 5274 5316 5348 1373 Size: 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1350 1283 545 396 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2656 3945 4191 669 Size: 988 281 718 1289 246 3079 Fragments arranged by size: 3079 1289 988 718 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6601 HpaI GTT'AAC Cuts at: 2656 4191 2656 Size: 1535 5066 KpnI G_GTAC'C Cuts at: 3892 3926 3892 Size: 34 6567 MfeI C'AATT_G Cuts at: 2517 4200 2517 Size: 1683 4918 MluI A'CGCG_T Cuts at: 6477 6477 Size: 6601 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2645 5715 5899 1305 Size: 1340 3070 184 2007 Fragments arranged by size: 3070 2007 1340 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4779 Fragments arranged by size: 4779 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2130 2163 2423 3363 3635 3771 Size: 648 867 97 33 260 940 272 136 Cuts at: 3771 4559 4918 5077 518 Size: 788 359 159 2042 Fragments arranged by size: 2042 940 867 788 648 359 272 260 159 136 97 33 MspA1I CmG'CkG Cuts at: 1606 2011 3378 3895 4017 4694 5842 6087 1606 Size: 405 1367 517 122 677 1148 245 2120 Fragments arranged by size: 2120 1367 1148 677 517 405 245 122 NcoI C'CATG_G Cuts at: 513 1065 2125 2418 3407 3766 513 Size: 552 1060 293 989 359 3348 Fragments arranged by size: 3348 1060 989 552 359 293 NdeI CA'TA_TG Cuts at: 387 387 Size: 6601 NheI G'CTAG_C Cuts at: 1052 2704 1052 Size: 1652 4949 NotI GC'GGCC_GC Cuts at: 4048 4048 Size: 6601 NsiI A_TGCA'T Cuts at: 3069 3069 Size: 6601 NspI r_CATG'y Cuts at: 1132 1229 3959 5504 1132 Size: 97 2730 1545 2229 Fragments arranged by size: 2730 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6601 PciI A'CATG_T Cuts at: 5500 5500 Size: 6601 PflMI CCAn_nnn'nTGG Cuts at: 1108 3108 3898 1108 Size: 2000 790 3811 Fragments arranged by size: 3811 2000 790 PmeI GTTT'AAAC Cuts at: 3905 3905 Size: 6601 PmlI CAC'GTG Cuts at: 3582 3582 Size: 6601 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4670 4702 6183 6215 4670 Size: 32 1481 32 5056 Fragments arranged by size: 5056 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2368 2368 Size: 6601 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6601 PsiI TTA'TAA Cuts at: 2121 4171 2121 Size: 2050 4551 PstI C_TGCA'G Cuts at: 830 3323 3953 830 Size: 2493 630 3478 Fragments arranged by size: 3478 2493 630 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6601 PvuII CAG'CTG Cuts at: 3895 3895 Size: 6601 SalI G'TCGA_C Cuts at: 1936 3943 1936 Size: 2007 4594 SapI GCTCTTCn'nnn_ Cuts at: 3249 3249 Size: 6601 SbfI CC_TGCA'GG Cuts at: 3953 3953 Size: 6601 ScaI AGT'ACT Cuts at: 4849 6561 4849 Size: 1712 4889 SfcI C'TryA_G Cuts at: 826 1045 3024 3319 3949 5084 5765 5956 826 Size: 219 1979 295 630 1135 681 191 1471 Fragments arranged by size: 1979 1471 1135 681 630 295 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6601 SmaI CCC'GGG Cuts at: 1872 3928 1872 Size: 2056 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4705 5606 5868 820 Size: 197 926 2762 901 262 1553 Fragments arranged by size: 2762 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4392 493 Size: 3899 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6601 SphI G_CATG'C Cuts at: 1132 3959 1132 Size: 2827 3774 SspI AAT'ATT Cuts at: 5 52 2977 3784 4525 5 Size: 47 2925 807 741 2081 Fragments arranged by size: 2925 2081 807 741 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6601 StyI C'CwwG_G Cuts at: 513 1065 2125 2418 2659 3082 3407 3543 3590 Size: 552 1060 293 241 423 325 136 47 Cuts at: 3590 3766 4040 513 Size: 176 274 3074 Fragments arranged by size: 3074 1060 552 423 325 293 274 241 176 136 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 3400 3625 4628 4645 4798 4983 Size: 1255 526 1294 225 1003 17 153 185 Cuts at: 4983 5428 325 Size: 445 1498 Fragments arranged by size: 1498 1294 1255 1003 526 445 225 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3028 4847 6559 Size: 274 80 33 51 1443 1051 1819 1712 Cuts at: 6559 96 Size: 138 Fragments arranged by size: 1819 1712 1443 1051 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6561 6593 6561 Size: 32 6569 Tth111I GACn'n_nGTC Cuts at: 1316 2186 1316 Size: 870 5731 XbaI T'CTAG_A Cuts at: 3937 3937 Size: 6601 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2123 2123 Size: 6601 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6601 XmaI C'CCGG_G Cuts at: 1870 3926 1870 Size: 2056 4545 XmnI GAAnn'nnTTC Cuts at: 4730 4730 Size: 6601 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4409 276 Size: 53 83 186 936 2875 2468 Fragments arranged by size: 2875 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI