Restriction map for
pFN21AA0943
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII BalI BamHI
BbrPI BclI BfrI BglI BlpI
BpmI Bpu1102I BsaBI BseAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MspA1I MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1087 GACGTC GACGT^C
AccII 930 CGCG CG^CG
ApaI 430 GGGCCC GGGCC^C
Asp700I 49 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 273 CCTAGG C^CTAGG
BbeI 571 GGCGCC GGCGC^C
BbvCI 628 GCTGAGG CCTCAGC(-5/-2)
BcgI 920 GCATGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 80 GGATAC GTATCC(6/5)
BcnI 882 CCCGG CC^[GC]GG
BglII 122 AGATCT A^GATCT
BlnI 273 CCTAGG C^CTAGG
BsaWI 32 TCCGGT [AT]^CCGG[AT]
BseRI 814 GAGGAG GAGGAG(10/8)
BsiEI 597 CGACCG CG[GA][CT]^CG
BsmBI 721 GAGACG CGTCTC(1/5)
BsmI 340 GCATTC GAATGC(1/-1)
BspHI 740 TCATGA T^CATGA
BstEII 633 GGTCACC G^GT[ACGT]ACC
BstF5I 879 CATCC GGATG(2/0)
BstPI 633 GGTCACC G^GT[ACGT]ACC
BstUI 930 CGCG CG^CG
BstXI 947 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 122 AGATCT [GA]^GATC[CT]
EarI 303 GAAGAG CTCTTC(1/4)
EcoO65I 633 GGTCACC G^GT[ACGT]ACC
FauI 909 CCCGC CCCGC(4/6)
FokI 879 CATCC GGATG(9/13)
HindIII 460 AAGCTT A^AGCTT
KasI 571 GGCGCC G^GCGCC
Ksp632I 303 GAAGAG CTCTTC(1/4)
MflI 122 AGATCT [GA]^GATC[CT]
MvnI 930 CGCG CG^CG
NaeI 266 GCCGGC GCC^GGC
NarI 571 GGCGCC GG^CGCC
NciI 882 CCCGG CC^[GC]GG
NcoI 652 CCATGG C^CATGG
NgoMIV 266 GCCGGC G^CCGGC
NspI 221 GCATGC [GA]CATG^[CT]
PleI 1144 GACTC GAGTC(4/5)
PpuMI 449 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 587 CTGCAG CTGCA^G
RcaI 740 TCATGA T^CATGA
SfcI 587 CTGCAG C^T[GA][CT]AG
SfoI 571 GGCGCC GGC^GCC
SphI 221 GCATGC GCATG^C
StuI 710 AGGCCT AGG^CCT
Tsp45I 634 GTCAC ^GT[GC]AC
XbaI 142 TCTAGA T^CTAGA
XcmI 1040 CCATGTTTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 122 AGATCT [GA]^GATC[CT]
XmnI 49 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 426 GTAC GT^AC
667 GTAC
AlwNI 448 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
751 CAGTCCCTG
AspHI 483 GAGCTC G[AT]GC[AT]^C
817 GAGCTC
BbsI 1156 GAAGAC GAAGAC(2/6)
469 GTCTTC
BpuAI 1156 GAAGAC GAAGAC(2/6)
469 GTCTTC
BsaAI 477 TACGTG [CT]AC^GT[GA]
715 TACGTG
BsaI 61 GAGACC GGTCTC(1/5)
657 GAGACC
BsgI 546 GTGCAG GTGCAG(16/14)
566 GTGCAG
BsiHKAI 483 GAGCTC G[AT]GC[AT]^C
817 GAGCTC
BsrDI 501 CATTGC GCAATG(2/0)
505 GCAATG
BsrFI 266 GCCGGC [GA]^CCGG[CT]
599 ACCGGC
Cfr10I 266 GCCGGC [GA]^CCGG[CT]
599 ACCGGC
DpnII 123 GATC ^GATC
243 GATC
DraII 192 AGGCCCC [GA]G^G[ACGT]CC[CT]
449 AGGTCCT
DsaI 652 CCATGG C^C[GA][CT]GG
854 CCGTGG
EcoO109I 192 AGGCCCC [GA]G^G[ACGT]CC[CT]
449 AGGTCCT
HaeII 571 GGCGCC [GA]GCGC^[CT]
784 AGCGCC
MaeIII 362 GTTAC ^GT[ACGT]AC
634 GTCAC
MboI 123 GATC ^GATC
243 GATC
NdeII 123 GATC ^GATC
243 GATC
RsaI 426 GTAC GT^AC
667 GTAC
SacI 483 GAGCTC GAGCT^C
817 GAGCTC
Sau3AI 123 GATC ^GATC
243 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BanII BbvI BfaI BmyI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI EcoRII EcoT14I
Fnu4HI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MboII MnlI
MslI MspI MvaI MwoI NlaIII
NlaIV PflMI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI TspRI
TthHB8I Van91I