(Circular) (Six-base) MAPSORT of: pFN21AA0943.seq Check: 8633 from: 1 to: 5884 pFN21AA0943 5884 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3096 3694 278 Size: 53 83 186 936 1560 598 2468 Fragments arranged by size: 2468 1560 936 598 186 83 53 AccI GT'mk_AC Cuts at: 1937 3227 1937 Size: 1290 4594 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2314 2480 2753 3000 3947 5331 5599 1632 Size: 682 166 273 247 947 1384 268 1917 Fragments arranged by size: 1917 1384 947 682 273 268 247 166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 4783 5760 1203 Size: 3580 977 1327 Fragments arranged by size: 3580 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 2525 1166 Size: 1359 4525 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5884 AlwNI CAG_nnn'CTG Cuts at: 2458 2761 3627 5199 2458 Size: 303 866 1572 3143 Fragments arranged by size: 3143 1572 866 303 ApaI G_GGCC'C Cuts at: 2439 2439 Size: 5884 ApaLI G'TGCA_C Cuts at: 3941 5097 3941 Size: 1156 4728 ApoI r'AATT_y Cuts at: 1493 3193 3419 3571 5680 1493 Size: 1700 226 152 2109 1697 Fragments arranged by size: 2109 1700 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 3209 1870 Size: 73 1266 4545 Fragments arranged by size: 4545 1266 73 AvrII C'CTAG_G Cuts at: 2278 2278 Size: 5884 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2274 2576 3000 3035 3205 Size: 325 217 627 487 302 424 35 170 Cuts at: 3205 4660 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 487 424 325 302 217 170 35 BanII G_rGCy'C Cuts at: 1391 2079 2439 2492 2700 2826 2868 3203 1391 Size: 688 360 53 208 126 42 335 4072 Fragments arranged by size: 4072 688 360 335 208 126 53 42 BbeI G_GCGC'C Cuts at: 2580 2580 Size: 5884 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2467 3168 928 Size: 971 568 701 3644 Fragments arranged by size: 3644 971 701 568 BbvCI CC'TCA_GC Cuts at: 2634 2634 Size: 5884 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2914 2948 4075 4109 1903 Size: 34 977 34 1127 34 3678 Fragments arranged by size: 3678 1127 977 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2079 3776 4986 2079 Size: 1697 1210 2977 Fragments arranged by size: 2977 1697 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4495 136 Size: 107 122 71 631 605 2823 1525 Fragments arranged by size: 2823 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2127 5879 2127 Size: 3752 2132 BlpI GC'TnA_GC Cuts at: 3301 3301 Size: 5884 Bme1580I G_kGCm'C Cuts at: 1790 2439 3040 3945 5101 1790 Size: 649 601 905 1156 2573 Fragments arranged by size: 2573 1156 905 649 601 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5884 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2099 2749 3615 Size: 416 53 83 399 24 682 650 866 Cuts at: 3615 4573 442 Size: 958 1753 Fragments arranged by size: 1753 958 866 682 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4544 1239 Size: 3305 2579 Bpu10I CC'TnA_GC Cuts at: 2634 2634 Size: 5884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4009 4874 5172 4009 Size: 865 298 4721 Fragments arranged by size: 4721 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2060 2656 4547 882 Size: 617 561 596 1891 2219 Fragments arranged by size: 2219 1891 617 596 561 BsaAI yAC'GTr Cuts at: 493 1204 2484 2722 3675 493 Size: 711 1280 238 953 2702 Fragments arranged by size: 2702 1280 953 711 238 BsaBI GATnn'nnATC Cuts at: 5878 5878 Size: 5884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2577 2766 3093 3691 Size: 53 83 186 936 1044 189 327 598 Cuts at: 3691 4073 4708 275 Size: 382 635 1451 Fragments arranged by size: 1451 1044 936 635 598 382 327 189 186 83 53 BsaWI w'CCGG_w Cuts at: 2037 4317 4715 4989 5136 5547 2037 Size: 2280 398 274 147 411 2374 Fragments arranged by size: 2374 2280 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2622 2652 1178 Size: 30 165 30 1219 30 4410 Fragments arranged by size: 4410 1219 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2834 1186 Size: 1648 4236 BseYI C'CCAG_C Cuts at: 2217 5087 5583 2217 Size: 2870 496 2518 Fragments arranged by size: 2870 2518 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2572 2592 1163 Size: 573 836 20 4455 Fragments arranged by size: 4455 836 573 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2605 3334 4095 5123 1540 Size: 275 186 604 729 761 1028 2301 Fragments arranged by size: 2301 1028 761 729 604 275 186 BsiHKAI G_wGCw'C Cuts at: 2492 2826 3945 4030 5101 2492 Size: 334 1119 85 1071 3275 Fragments arranged by size: 3275 1119 1071 334 85 BsmI GAATG_Cn' Cuts at: 2345 3395 3488 2345 Size: 1050 93 4741 Fragments arranged by size: 4741 1050 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2720 2720 Size: 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2439 2492 2700 2826 2868 3040 Size: 399 289 360 53 208 126 42 172 Cuts at: 3040 3203 3945 4030 5101 1391 Size: 163 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 289 208 172 163 126 85 53 42 BspEI T'CCGG_A Cuts at: 5547 5547 Size: 5884 BspHI T'CATG_A Cuts at: 2745 3773 5503 2745 Size: 1028 1730 3126 Fragments arranged by size: 3126 1730 1028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3239 844 Size: 2395 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3771 1394 Size: 429 1948 3507 Fragments arranged by size: 3507 1948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 2517 4379 4553 65 Size: 1183 1257 12 1862 174 1396 Fragments arranged by size: 1862 1396 1257 1183 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2271 2604 4528 4715 1700 Size: 110 461 333 1924 187 2869 Fragments arranged by size: 2869 1924 461 333 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 3944 4956 3944 Size: 1012 4872 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5884 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 2638 1184 Size: 1454 4430 BstXI CCAn_nnnn'nTGG Cuts at: 2959 2959 Size: 5884 BstYI r'GATC_y Cuts at: 1972 2127 3214 3581 3966 3983 5435 5521 5533 Size: 155 1087 367 385 17 1452 86 12 Cuts at: 5533 5879 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1087 385 367 346 155 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2657 2859 513 Size: 552 939 653 202 3538 Fragments arranged by size: 3538 939 653 552 202 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2917 2938 487 Size: 683 56 1691 21 3433 Fragments arranged by size: 3433 1691 683 56 21 BtsI GCAGTG_nn' Cuts at: 2580 2608 2737 2902 3400 4194 4214 2580 Size: 28 129 165 498 794 20 4250 Fragments arranged by size: 4250 794 498 165 129 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4891 809 Size: 507 3575 1802 Fragments arranged by size: 3575 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3331 4220 5849 Size: 54 1425 50 275 18 1501 889 1629 Cuts at: 5849 8 Size: 43 Fragments arranged by size: 1629 1501 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3331 1537 Size: 275 1519 4090 Fragments arranged by size: 4090 1519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2303 3814 1353 Size: 950 1511 3423 Fragments arranged by size: 3423 1511 950 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4471 4845 4991 387 Size: 1064 138 2882 374 146 1280 Fragments arranged by size: 2882 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2490 2824 2490 Size: 334 5550 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2314 2480 2753 3000 3947 4544 5331 Size: 393 682 166 273 247 947 597 787 Cuts at: 5331 5599 1239 Size: 268 1524 Fragments arranged by size: 1524 947 787 682 597 393 273 268 247 166 EcoO109I rG'GnC_Cy Cuts at: 2198 2455 2198 Size: 257 5627 EcoRI G'AATT_C Cuts at: 3193 3571 3193 Size: 378 5506 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2758 2758 Size: 5884 FspI TGC'GCA Cuts at: 4390 4390 Size: 5884 HaeII r_GCGC'y Cuts at: 1678 2580 2793 5031 1678 Size: 902 213 2238 2531 Fragments arranged by size: 2531 2238 902 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2129 2149 2161 2232 Size: 32 135 32 545 12 20 12 71 Cuts at: 2232 2264 2622 2654 4525 4557 4599 4631 1373 Size: 32 358 32 1871 32 42 32 2626 Fragments arranged by size: 2626 1871 545 358 135 71 42 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3228 3474 669 Size: 988 281 1290 246 3079 Fragments arranged by size: 3079 1290 988 281 246 HindIII A'AGCT_T Cuts at: 748 2465 748 Size: 1717 4167 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KasI G'GCGC_C Cuts at: 2576 2576 Size: 5884 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2625 4998 5182 1305 Size: 1320 2373 184 2007 Fragments arranged by size: 2373 2007 1320 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4062 Fragments arranged by size: 4062 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2510 2525 2957 3842 4201 4360 518 Size: 648 1344 15 432 885 359 159 2042 Fragments arranged by size: 2042 1344 885 648 432 359 159 15 MspA1I CmG'CkG Cuts at: 1606 3300 3977 5125 5370 1606 Size: 1694 677 1148 245 2120 Fragments arranged by size: 2120 1694 1148 677 245 NaeI GCC'GGC Cuts at: 2273 2273 Size: 5884 NarI GG'CG_CC Cuts at: 2577 2577 Size: 5884 NcoI C'CATG_G Cuts at: 513 1065 2004 2657 513 Size: 552 939 653 3740 Fragments arranged by size: 3740 939 653 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NgoMIV G'CCGG_C Cuts at: 2271 2271 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 2230 3242 4787 1132 Size: 97 1001 1012 1545 2229 Fragments arranged by size: 2229 1545 1012 1001 97 PasI CC'CwG_GG Cuts at: 1348 2761 1348 Size: 1413 4471 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 2186 2263 2761 1108 Size: 1078 77 498 4231 Fragments arranged by size: 4231 1078 498 77 PfoI T'CCnGG_A Cuts at: 2886 2886 Size: 5884 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2455 2455 Size: 5884 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PspOMI G'GGCC_C Cuts at: 2435 2435 Size: 5884 PstI C_TGCA'G Cuts at: 830 2596 3236 830 Size: 1766 640 3478 Fragments arranged by size: 3478 1766 640 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 SacI G_AGCT'C Cuts at: 2492 2826 2492 Size: 334 5550 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 2592 3232 4367 5048 5239 826 Size: 219 1547 640 1135 681 191 1471 Fragments arranged by size: 1547 1471 1135 681 640 219 191 SfoI GGC'GCC Cuts at: 2578 2578 Size: 5884 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 3211 1872 Size: 1339 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3988 4889 5151 820 Size: 197 926 2045 901 262 1553 Fragments arranged by size: 2045 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 2230 3242 1132 Size: 1098 1012 3774 Fragments arranged by size: 3774 1098 1012 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 2717 1517 Size: 1200 4684 StyI C'CwwG_G Cuts at: 513 1065 2004 2278 2493 2657 3323 513 Size: 552 939 274 215 164 666 3074 Fragments arranged by size: 3074 939 666 552 274 215 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3911 3928 4081 4266 4711 325 Size: 1255 2331 17 153 185 445 1498 Fragments arranged by size: 2331 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4130 5842 96 Size: 274 80 33 51 1443 2153 1712 138 Fragments arranged by size: 2153 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2572 2604 5844 5876 2572 Size: 32 3240 32 2580 Fragments arranged by size: 3240 2580 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5884 XbaI T'CTAG_A Cuts at: 2147 3220 2147 Size: 1073 4811 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3052 3052 Size: 5884 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 3209 1870 Size: 1339 4545 XmnI GAAnn'nnTTC Cuts at: 2058 4013 2058 Size: 1955 3929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3094 3692 276 Size: 53 83 186 936 1560 598 2468 Fragments arranged by size: 2468 1560 936 598 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI