Restriction map for
pFN21AA0943
(Linear) (Six-base) MAPSORT of: KIAA0943.seq Check: 7152 from: 1 to: 1179
KIAA0943.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AatII G_ACGT'C
Cuts at: 0 1091 1179
Size: 1091 88
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 475 748 995 1179
Size: 309 166 273 247 184
Fragments arranged by size:
309 273 247 184 166
AleI CACnn'nnGTG
Cuts at: 0 520 1179
Size: 520 659
AlwNI CAG_nnn'CTG
Cuts at: 0 453 756 1179
Size: 453 303 423
Fragments arranged by size:
453 423 303
ApaI G_GGCC'C
Cuts at: 0 434 1179
Size: 434 745
AvrII C'CTAG_G
Cuts at: 0 273 1179
Size: 273 906
BanI G'GyrC_C
Cuts at: 0 269 571 995 1030 1179
Size: 269 302 424 35 149
Fragments arranged by size:
424 302 269 149 35
BanII G_rGCy'C
Cuts at: 0 74 434 487 695 821 863 1179
Size: 74 360 53 208 126 42 316
Fragments arranged by size:
360 316 208 126 74 53 42
BbeI G_GCGC'C
Cuts at: 0 575 1179
Size: 575 604
BbsI GAAGACnn'nnnn_
Cuts at: 0 462 1163 1179
Size: 462 701 16
Fragments arranged by size:
701 462 16
BbvCI CC'TCA_GC
Cuts at: 0 629 1179
Size: 629 550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 909 943 1179
Size: 909 34 236
Fragments arranged by size:
909 236 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 74 1179
Size: 74 1105
BglII A'GATC_T
Cuts at: 0 122 1179
Size: 122 1057
Bme1580I G_kGCm'C
Cuts at: 0 434 1035 1179
Size: 434 601 144
Fragments arranged by size:
601 434 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 744 1179
Size: 94 650 435
Fragments arranged by size:
650 435 94
Bpu10I CC'TnA_GC
Cuts at: 0 629 1179
Size: 629 550
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 651 1179
Size: 55 596 528
Fragments arranged by size:
596 528 55
BsaAI yAC'GTr
Cuts at: 0 479 717 1179
Size: 479 238 462
Fragments arranged by size:
479 462 238
BsaHI Gr'CG_yC
Cuts at: 0 572 761 1088 1179
Size: 572 189 327 91
Fragments arranged by size:
572 327 189 91
BsaWI w'CCGG_w
Cuts at: 0 32 1179
Size: 32 1147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 617 647 1179
Size: 617 30 532
Fragments arranged by size:
617 532 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 829 1179
Size: 829 350
BseYI C'CCAG_C
Cuts at: 0 212 1179
Size: 212 967
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 587 1179
Size: 567 20 592
Fragments arranged by size:
592 567 20
BsiEI CG_ry'CG
Cuts at: 0 600 1179
Size: 600 579
BsiHKAI G_wGCw'C
Cuts at: 0 487 821 1179
Size: 487 334 358
Fragments arranged by size:
487 358 334
BsmI GAATG_Cn'
Cuts at: 0 340 1179
Size: 340 839
BsmBI CGTCTCn'nnnn_
Cuts at: 0 715 1179
Size: 715 464
Bsp1286I G_dGCh'C
Cuts at: 0 74 434 487 695 821 863 1035 1179
Size: 74 360 53 208 126 42 172 144
Fragments arranged by size:
360 208 172 144 126 74 53 42
BspHI T'CATG_A
Cuts at: 0 740 1179
Size: 740 439
BsrDI GCAATG_nn'
Cuts at: 0 500 512 1179
Size: 500 12 667
Fragments arranged by size:
667 500 12
BsrFI r'CCGG_y
Cuts at: 0 266 599 1179
Size: 266 333 580
Fragments arranged by size:
580 333 266
BstEII G'GTnAC_C
Cuts at: 0 633 1179
Size: 633 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 954 1179
Size: 954 225
BstYI r'GATC_y
Cuts at: 0 122 1179
Size: 122 1057
BtgI C'CryG_G
Cuts at: 0 652 854 1179
Size: 652 202 325
Fragments arranged by size:
652 325 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 912 933 1179
Size: 912 21 246
Fragments arranged by size:
912 246 21
BtsI GCAGTG_nn'
Cuts at: 0 575 603 732 897 1179
Size: 575 28 129 165 282
Fragments arranged by size:
575 282 165 129 28
EarI CTCTTCn'nnn_
Cuts at: 0 298 1179
Size: 298 881
EcoICRI GAG'CTC
Cuts at: 0 485 819 1179
Size: 485 334 360
Fragments arranged by size:
485 360 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 475 748 995 1179
Size: 309 166 273 247 184
Fragments arranged by size:
309 273 247 184 166
EcoO109I rG'GnC_Cy
Cuts at: 0 193 450 1179
Size: 193 257 729
Fragments arranged by size:
729 257 193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 753 1179
Size: 753 426
HaeII r_GCGC'y
Cuts at: 0 575 788 1179
Size: 575 213 391
Fragments arranged by size:
575 391 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 124 144 156 227 259 617 649
Size: 112 12 20 12 71 32 358 32
Cuts at: 649 1179
Size: 530
Fragments arranged by size:
530 358 112 71 32 32 20 12
12
HindIII A'AGCT_T
Cuts at: 0 460 1179
Size: 460 719
KasI G'GCGC_C
Cuts at: 0 571 1179
Size: 571 608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1179
Size: 620 559
MslI CAynn'nnrTG
Cuts at: 0 505 520 952 1179
Size: 505 15 432 227
Fragments arranged by size:
505 432 227 15
NaeI GCC'GGC
Cuts at: 0 268 1179
Size: 268 911
NarI GG'CG_CC
Cuts at: 0 572 1179
Size: 572 607
NcoI C'CATG_G
Cuts at: 0 652 1179
Size: 652 527
NgoMIV G'CCGG_C
Cuts at: 0 266 1179
Size: 266 913
NspI r_CATG'y
Cuts at: 0 225 1179
Size: 225 954
PasI CC'CwG_GG
Cuts at: 0 756 1179
Size: 756 423
PflMI CCAn_nnn'nTGG
Cuts at: 0 181 258 756 1179
Size: 181 77 498 423
Fragments arranged by size:
498 423 181 77
PfoI T'CCnGG_A
Cuts at: 0 881 1179
Size: 881 298
PpuMI rG'GwC_Cy
Cuts at: 0 450 1179
Size: 450 729
PspOMI G'GGCC_C
Cuts at: 0 430 1179
Size: 430 749
PstI C_TGCA'G
Cuts at: 0 591 1179
Size: 591 588
SacI G_AGCT'C
Cuts at: 0 487 821 1179
Size: 487 334 358
Fragments arranged by size:
487 358 334
SfcI C'TryA_G
Cuts at: 0 587 1179
Size: 587 592
SfoI GGC'GCC
Cuts at: 0 573 1179
Size: 573 606
SphI G_CATG'C
Cuts at: 0 225 1179
Size: 225 954
StuI AGG'CCT
Cuts at: 0 712 1179
Size: 712 467
StyI C'CwwG_G
Cuts at: 0 273 488 652 1179
Size: 273 215 164 527
Fragments arranged by size:
527 273 215 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 567 599 1179
Size: 567 32 580
Fragments arranged by size:
580 567 32
XbaI T'CTAG_A
Cuts at: 0 142 1179
Size: 142 1037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1047 1179
Size: 1047 132
XmnI GAAnn'nnTTC
Cuts at: 0 53 1179
Size: 53 1126
ZraI GAC'GTC
Cuts at: 0 1089 1179
Size: 1089 90
Enzymes that do cut:
AatII AcuI AleI AlwNI ApaI AvrII BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglII Bme1580I BmrI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI
BsrFI BstEII BstXI BstYI BtgI BtgZI BtsI EarI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HindIII KasI
MmeI MslI NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpuMI PspOMI PstI SacI SfcI SfoI
SphI StuI StyI TstI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BclI BfrBI BglI BlpI BmgBI
BmtI BplI BpmI BpuEI BsaBI BsiWI BspEI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KpnI MfeI MluI MscI MspA1I NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII
TatI Tth111I XhoI XmaI