(Circular) (Six-base) MAPSORT of: pFN21AA0937.seq Check: 408 from: 1 to: 6562 pFN21AA0937 6562 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2761 2761 Size: 6562 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4372 278 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3333 3905 1937 Size: 1396 572 4594 Fragments arranged by size: 4594 1396 572 Acc65I G'GTAC_C Cuts at: 3883 3883 Size: 6562 AclI AA'CG_TT Cuts at: 1563 4689 5062 1563 Size: 3126 373 3063 Fragments arranged by size: 3126 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2774 3656 4625 6009 6277 1632 Size: 1142 882 969 1384 268 1917 Fragments arranged by size: 1917 1384 1142 969 882 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6365 AflIII A'CryG_T Cuts at: 1203 5461 6438 1203 Size: 4258 977 1327 Fragments arranged by size: 4258 1327 977 AgeI A'CCGG_T Cuts at: 5393 5393 Size: 6562 AhdI GACnn_n'nnGTC Cuts at: 2185 5291 2185 Size: 3106 3456 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6562 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2153 2681 1184 Size: 969 528 5065 Fragments arranged by size: 5065 969 528 AlwNI CAG_nnn'CTG Cuts at: 2708 2902 4305 5877 2708 Size: 194 1403 1572 3393 Fragments arranged by size: 3393 1572 1403 194 ApaI G_GGCC'C Cuts at: 2637 3278 2637 Size: 641 5921 ApaLI G'TGCA_C Cuts at: 4619 5775 4619 Size: 1156 5406 ApoI r'AATT_y Cuts at: 1493 3871 4097 4249 6358 1493 Size: 2378 226 152 2109 1697 Fragments arranged by size: 2378 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5116 160 Size: 872 4084 1606 Fragments arranged by size: 4084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 AvaI C'yCGr_G Cuts at: 1870 1943 2919 3579 3887 1870 Size: 73 976 660 308 4545 Fragments arranged by size: 4545 976 660 308 73 BamHI G'GATC_C Cuts at: 3012 3892 4259 3012 Size: 880 367 5315 Fragments arranged by size: 5315 880 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2469 2735 3210 3431 3683 Size: 325 217 627 682 266 475 221 252 Cuts at: 3683 3883 5338 618 Size: 200 1455 1842 Fragments arranged by size: 1842 1455 682 627 475 325 266 252 221 217 200 BanII G_rGCy'C Cuts at: 1391 2637 2822 3278 3881 1391 Size: 1246 185 456 603 4072 Fragments arranged by size: 4072 1246 603 456 185 BbeI G_GCGC'C Cuts at: 2473 2473 Size: 6562 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2762 3431 928 Size: 971 863 669 4059 Fragments arranged by size: 4059 971 863 669 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4753 4787 1903 Size: 34 50 34 2732 34 3678 Fragments arranged by size: 3678 2732 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4454 5664 4454 Size: 1210 5352 BclI T'GATC_A Cuts at: 1548 1548 Size: 6562 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5173 136 Size: 107 122 71 631 605 3501 1525 Fragments arranged by size: 3501 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6557 6557 Size: 6562 BlpI GC'TnA_GC Cuts at: 3979 3979 Size: 6562 Bme1580I G_kGCm'C Cuts at: 1790 2637 3278 4623 5779 1790 Size: 847 641 1345 1156 2573 Fragments arranged by size: 2573 1345 1156 847 641 BmgBI CAC'GTC Cuts at: 1318 3331 1318 Size: 2013 4549 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2222 2575 2640 Size: 416 53 83 399 24 805 353 65 Cuts at: 2640 2814 2908 2948 3018 4293 5251 442 Size: 174 94 40 70 1275 958 1753 Fragments arranged by size: 1753 1275 958 805 416 399 353 174 94 83 70 65 53 40 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6562 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3769 3769 Size: 6562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2697 2776 3426 3673 5222 1239 Size: 1458 79 650 247 1549 2579 Fragments arranged by size: 2579 1549 1458 650 247 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4687 5552 5850 4687 Size: 865 298 5399 Fragments arranged by size: 5399 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5225 882 Size: 617 3726 2219 Fragments arranged by size: 3726 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2404 4353 493 Size: 711 1200 1949 2702 Fragments arranged by size: 2702 1949 1200 711 BsaBI GATnn'nnATC Cuts at: 6556 6556 Size: 6562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2062 2312 2470 4369 Size: 53 83 186 936 529 250 158 1899 Cuts at: 4369 4751 5386 275 Size: 382 635 1451 Fragments arranged by size: 1899 1451 936 635 529 382 250 186 158 83 53 BsaWI w'CCGG_w Cuts at: 2778 2855 3507 3544 3607 4995 5393 5667 5814 Size: 77 652 37 63 1388 398 274 147 Cuts at: 5814 6225 2778 Size: 411 3115 Fragments arranged by size: 3115 1388 652 411 398 274 147 77 63 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 2288 2318 3424 Size: 30 165 30 834 30 21 30 1106 Cuts at: 3424 3454 1178 Size: 30 4286 Fragments arranged by size: 4286 1106 834 165 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2245 1186 Size: 1059 5503 BseYI C'CCAG_C Cuts at: 2074 2140 2445 5765 6261 2074 Size: 66 305 3320 496 2375 Fragments arranged by size: 3320 2375 496 305 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3045 3214 1163 Size: 573 1309 169 4511 Fragments arranged by size: 4511 1309 573 169 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 2056 4012 4773 5801 1540 Size: 275 186 21 34 1956 761 1028 2301 Fragments arranged by size: 2301 1956 1028 761 275 186 34 21 BsiHKAI G_wGCw'C Cuts at: 2052 4623 4708 5779 2052 Size: 2571 85 1071 2835 Fragments arranged by size: 2835 2571 1071 85 BsmI GAATG_Cn' Cuts at: 3534 4073 4166 3534 Size: 539 93 5930 Fragments arranged by size: 5930 539 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2165 2165 Size: 6562 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2637 2822 3278 3881 4623 4708 Size: 399 262 585 185 456 603 742 85 Cuts at: 4708 5779 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 603 585 456 399 262 185 85 BspEI T'CCGG_A Cuts at: 2778 3507 3607 6225 2778 Size: 729 100 2618 3115 Fragments arranged by size: 3115 2618 729 100 BspHI T'CATG_A Cuts at: 3018 4451 6181 3018 Size: 1433 1730 3399 Fragments arranged by size: 3399 1730 1433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2190 2761 2901 3410 3917 844 Size: 1346 571 140 509 507 3489 Fragments arranged by size: 3489 1346 571 509 507 140 BsrBI CCG'CTC Cuts at: 1394 1823 4449 1394 Size: 429 2626 3507 Fragments arranged by size: 3507 2626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2907 5057 5231 65 Size: 1183 1659 2150 174 1396 Fragments arranged by size: 2150 1659 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 2466 5206 5393 1700 Size: 110 301 355 2740 187 2869 Fragments arranged by size: 2869 2740 355 301 187 110 BsrGI T'GTAC_A Cuts at: 96 3360 96 Size: 3264 3298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6562 BssSI C'ACGA_G Cuts at: 3647 4622 5634 3647 Size: 975 1012 4575 Fragments arranged by size: 4575 1012 975 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6562 BstBI TT'CG_AA Cuts at: 5366 5366 Size: 6562 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6562 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2722 3241 3793 2011 Size: 711 519 552 4780 Fragments arranged by size: 4780 711 552 519 BstYI r'GATC_y Cuts at: 1972 3012 3892 4259 4644 4661 6113 6199 6211 Size: 1040 880 367 385 17 1452 86 12 Cuts at: 6211 6557 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1040 880 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2511 3262 2511 Size: 751 5811 BtgI C'CryG_G Cuts at: 513 1065 2022 2421 2490 2613 2814 3769 513 Size: 552 957 399 69 123 201 955 3306 Fragments arranged by size: 3306 957 955 552 399 201 123 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2751 487 Size: 683 56 1525 4298 Fragments arranged by size: 4298 1525 683 56 BtsI GCAGTG_nn' Cuts at: 2696 3398 3843 4078 4872 4892 2696 Size: 702 445 235 794 20 4366 Fragments arranged by size: 4366 794 702 445 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6527 DraI TTT'AAA Cuts at: 3866 3866 Size: 6562 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5569 809 Size: 507 4253 1802 Fragments arranged by size: 4253 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2053 2525 Size: 54 1425 50 275 18 189 34 472 Cuts at: 2525 2871 2904 3325 3484 3766 4009 4898 6527 Size: 346 33 421 159 282 243 889 1629 Cuts at: 6527 8 Size: 43 Fragments arranged by size: 1629 1425 889 472 421 346 282 275 243 189 159 54 50 43 34 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2019 2053 4009 1537 Size: 275 207 34 1956 4090 Fragments arranged by size: 4090 1956 275 207 34 EarI CTCTTCn'nnn_ Cuts at: 1353 3560 4492 1353 Size: 2207 932 3423 Fragments arranged by size: 3423 2207 932 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2192 2213 2453 5149 5523 5669 Size: 1064 138 603 21 240 2696 374 146 Cuts at: 5669 387 Size: 1280 Fragments arranged by size: 2696 1280 1064 603 374 240 146 138 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2697 2774 2776 3426 3656 3673 4625 Size: 393 1065 77 2 650 230 17 952 Cuts at: 4625 5222 6009 6277 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1065 952 787 650 597 393 268 230 77 17 2 EcoO109I rG'GnC_Cy Cuts at: 2502 2634 2840 2941 3274 2502 Size: 132 206 101 333 5790 Fragments arranged by size: 5790 333 206 132 101 EcoRI G'AATT_C Cuts at: 3871 4249 3871 Size: 378 6184 FspI TGC'GCA Cuts at: 5068 5068 Size: 6562 HaeII r_GCGC'y Cuts at: 1678 2452 2473 3579 5709 1678 Size: 774 21 1106 2130 2531 Fragments arranged by size: 2531 2130 1106 774 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2240 2272 2288 Size: 32 135 32 599 32 37 32 16 Cuts at: 2288 2320 2422 2454 5203 5235 5277 5309 1373 Size: 32 102 32 2749 32 42 32 2626 Fragments arranged by size: 2749 2626 599 135 102 42 37 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2986 3906 4152 669 Size: 988 281 1048 920 246 3079 Fragments arranged by size: 3079 1048 988 920 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6562 HpaI GTT'AAC Cuts at: 4152 4152 Size: 6562 KasI G'GCGC_C Cuts at: 2469 2469 Size: 6562 KpnI G_GTAC'C Cuts at: 3887 3887 Size: 6562 MfeI C'AATT_G Cuts at: 4161 4161 Size: 6562 MluI A'CGCG_T Cuts at: 6438 6438 Size: 6562 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3147 3422 5676 5860 1305 Size: 1842 275 2254 184 2007 Fragments arranged by size: 2254 2007 1842 275 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2527 2873 2906 3486 3768 Size: 54 1425 343 695 346 33 580 282 Cuts at: 3768 10 Size: 2804 Fragments arranged by size: 2804 1425 695 580 346 343 282 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2327 2352 3239 4520 4879 5038 518 Size: 648 1161 25 887 1281 359 159 2042 Fragments arranged by size: 2042 1281 1161 887 648 359 159 25 MspA1I CmG'CkG Cuts at: 1606 2058 2078 2644 3029 3978 4655 5803 6048 Size: 452 20 566 385 949 677 1148 245 Cuts at: 6048 1606 Size: 2120 Fragments arranged by size: 2120 1148 949 677 566 452 385 245 20 NaeI GCC'GGC Cuts at: 2113 2468 2113 Size: 355 6207 NarI GG'CG_CC Cuts at: 2470 2470 Size: 6562 NcoI C'CATG_G Cuts at: 513 1065 2421 2490 2814 3769 513 Size: 552 1356 69 324 955 3306 Fragments arranged by size: 3306 1356 955 552 324 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 6562 NgoMIV G'CCGG_C Cuts at: 2111 2466 2111 Size: 355 6207 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6562 NotI GC'GGCC_GC Cuts at: 4009 4009 Size: 6562 NspI r_CATG'y Cuts at: 1132 1229 2351 2668 2800 3920 5465 1132 Size: 97 1122 317 132 1120 1545 2229 Fragments arranged by size: 2229 1545 1122 1120 317 132 97 PasI CC'CwG_GG Cuts at: 1348 2264 2537 3113 3823 1348 Size: 916 273 576 710 4087 Fragments arranged by size: 4087 916 710 576 273 PciI A'CATG_T Cuts at: 5461 5461 Size: 6562 PflMI CCAn_nnn'nTGG Cuts at: 1108 2708 1108 Size: 1600 4962 PfoI T'CCnGG_A Cuts at: 2306 2674 2306 Size: 368 6194 PmeI GTTT'AAAC Cuts at: 3866 3866 Size: 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4663 6144 6176 4631 Size: 32 1481 32 5017 Fragments arranged by size: 5017 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2502 2840 2941 2502 Size: 338 101 6123 Fragments arranged by size: 6123 338 101 PshAI GACnn'nnGTC Cuts at: 1613 2157 1613 Size: 544 6018 PsiI TTA'TAA Cuts at: 4132 4132 Size: 6562 PspOMI G'GGCC_C Cuts at: 2633 3274 2633 Size: 641 5921 PstI C_TGCA'G Cuts at: 830 2187 2898 3914 830 Size: 1357 711 1016 3478 Fragments arranged by size: 3478 1357 1016 711 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6562 PvuII CAG'CTG Cuts at: 2644 2644 Size: 6562 SalI G'TCGA_C Cuts at: 1936 3904 1936 Size: 1968 4594 SanDI GG'GwC_CC Cuts at: 2941 2941 Size: 6562 SbfI CC_TGCA'GG Cuts at: 3914 3914 Size: 6562 ScaI AGT'ACT Cuts at: 4810 6522 4810 Size: 1712 4850 SexAI A'CCwGG_T Cuts at: 3300 3300 Size: 6562 SfcI C'TryA_G Cuts at: 826 1045 2068 2183 2639 2894 3197 3517 3910 Size: 219 1023 115 456 255 303 320 393 Cuts at: 3910 5045 5726 5917 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1023 681 456 393 320 303 255 219 191 115 SfoI GGC'GCC Cuts at: 2471 2471 Size: 6562 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 SgrAI Cr'CCGG_yG Cuts at: 1700 2466 1700 Size: 766 5796 SmaI CCC'GGG Cuts at: 1872 2921 3889 1872 Size: 1049 968 4545 Fragments arranged by size: 4545 1049 968 SmlI C'TyrA_G Cuts at: 820 1017 1943 4666 5567 5829 820 Size: 197 926 2723 901 262 1553 Fragments arranged by size: 2723 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2404 4353 493 Size: 1911 1949 2702 Fragments arranged by size: 2702 1949 1911 SpeI A'CTAG_T Cuts at: 152 152 Size: 6562 SphI G_CATG'C Cuts at: 1132 2351 2800 3920 1132 Size: 1219 449 1120 3774 Fragments arranged by size: 3774 1219 1120 449 SspI AAT'ATT Cuts at: 5 52 4486 5 Size: 47 4434 2081 Fragments arranged by size: 4434 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6562 StyI C'CwwG_G Cuts at: 513 1065 2421 2490 2814 2994 3769 4001 513 Size: 552 1356 69 324 180 775 232 3074 Fragments arranged by size: 3074 1356 775 552 324 232 180 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3090 3228 3449 3504 4589 4606 4759 Size: 1255 1510 138 221 55 1085 17 153 Cuts at: 4759 4944 5389 325 Size: 185 445 1498 Fragments arranged by size: 1510 1498 1255 1085 445 221 185 153 138 55 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3360 4808 6520 Size: 274 80 33 51 1443 1383 1448 1712 Cuts at: 6520 96 Size: 138 Fragments arranged by size: 1712 1448 1443 1383 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3424 3456 6522 6554 3424 Size: 32 3066 32 3432 Fragments arranged by size: 3432 3066 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3713 1316 Size: 2397 4165 XbaI T'CTAG_A Cuts at: 3898 3898 Size: 6562 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3479 3479 Size: 6562 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6562 XmaI C'CCGG_G Cuts at: 1870 2919 3887 1870 Size: 1049 968 4545 Fragments arranged by size: 4545 1049 968 XmnI GAAnn'nnTTC Cuts at: 2198 3569 4691 2198 Size: 1371 1122 4069 Fragments arranged by size: 4069 1371 1122 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4370 276 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI