(Circular) (Six-base) MAPSORT of: pFN21AA0930.seq Check: 4433 from: 1 to: 5944 pFN21AA0930 5944 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3754 278 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 3287 1937 Size: 879 471 4594 Fragments arranged by size: 4594 879 471 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 4071 4444 1563 Size: 2508 373 3063 Fragments arranged by size: 3063 2508 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2865 2878 3143 4007 5391 5659 1632 Size: 1233 13 265 864 1384 268 1917 Fragments arranged by size: 1917 1384 1233 864 268 265 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 2399 2619 4843 5820 1203 Size: 1196 220 2224 977 1327 Fragments arranged by size: 2224 1327 1196 977 220 AgeI A'CCGG_T Cuts at: 4775 4775 Size: 5944 AhdI GACnn_n'nnGTC Cuts at: 4673 4673 Size: 5944 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5944 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5944 AlwNI CAG_nnn'CTG Cuts at: 2368 3687 5259 2368 Size: 1319 1572 3053 Fragments arranged by size: 3053 1572 1319 ApaI G_GGCC'C Cuts at: 2774 2774 Size: 5944 ApaLI G'TGCA_C Cuts at: 2395 4001 5157 2395 Size: 1606 1156 3182 Fragments arranged by size: 3182 1606 1156 ApoI r'AATT_y Cuts at: 1493 3253 3479 3631 5740 1493 Size: 1760 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4498 160 Size: 872 3466 1606 Fragments arranged by size: 3466 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 3269 1870 Size: 73 1326 4545 Fragments arranged by size: 4545 1326 73 BamHI G'GATC_C Cuts at: 3274 3641 3274 Size: 367 5577 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 3265 4720 618 Size: 325 217 627 1175 303 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 303 217 BanII G_rGCy'C Cuts at: 1391 2167 2648 2774 3263 1391 Size: 776 481 126 489 4072 Fragments arranged by size: 4072 776 489 481 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2113 928 Size: 971 214 4759 Fragments arranged by size: 4759 971 214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2213 2247 4135 4169 1903 Size: 34 276 34 1888 34 3678 Fragments arranged by size: 3678 1888 276 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 1548 Size: 5944 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2056 2708 2778 Size: 107 122 71 631 605 384 652 70 Cuts at: 2778 2798 4555 136 Size: 20 1757 1525 Fragments arranged by size: 1757 1525 652 631 605 384 122 107 71 70 20 BglII A'GATC_T Cuts at: 5939 5939 Size: 5944 BlpI GC'TnA_GC Cuts at: 2253 3361 2253 Size: 1108 4836 Bme1580I G_kGCm'C Cuts at: 1790 2399 2774 2967 3095 4005 5161 1790 Size: 609 375 193 128 910 1156 2573 Fragments arranged by size: 2573 1156 910 609 375 193 128 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5944 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2338 2457 3675 Size: 416 53 83 399 24 921 119 1218 Cuts at: 3675 4633 442 Size: 958 1753 Fragments arranged by size: 1753 1218 958 921 416 399 119 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2839 4604 1239 Size: 1600 1765 2579 Fragments arranged by size: 2579 1765 1600 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 2936 4069 4934 5232 2651 Size: 285 1133 865 298 3363 Fragments arranged by size: 3363 1133 865 298 285 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2307 3081 4607 882 Size: 617 808 774 1526 2219 Fragments arranged by size: 2219 1526 808 774 617 BsaAI yAC'GTr Cuts at: 493 1204 2402 3735 493 Size: 711 1198 1333 2702 Fragments arranged by size: 2702 1333 1198 711 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3751 4133 4768 275 Size: 53 83 186 936 2218 382 635 1451 Fragments arranged by size: 2218 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2973 4377 4775 5049 5196 5607 2973 Size: 1404 398 274 147 411 3310 Fragments arranged by size: 3310 1404 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2568 2598 1178 Size: 30 165 30 1165 30 4524 Fragments arranged by size: 4524 1165 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2507 2558 3032 1186 Size: 1162 159 51 474 4098 Fragments arranged by size: 4098 1162 474 159 51 BseYI C'CCAG_C Cuts at: 2860 5147 5643 2860 Size: 2287 496 3161 Fragments arranged by size: 3161 2287 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2253 3038 1163 Size: 573 517 785 4069 Fragments arranged by size: 4069 785 573 517 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 4155 5183 1540 Size: 275 186 1393 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2399 4005 4090 5161 2399 Size: 1606 85 1071 3182 Fragments arranged by size: 3182 1606 1071 85 BsmI GAATG_Cn' Cuts at: 3455 3548 3455 Size: 93 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2167 2399 2648 2774 2967 3095 3263 Size: 399 377 232 249 126 193 128 168 Cuts at: 3263 4005 4090 5161 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 377 249 232 193 168 128 126 85 BspEI T'CCGG_A Cuts at: 5607 5607 Size: 5944 BspHI T'CATG_A Cuts at: 2526 3833 5563 2526 Size: 1307 1730 2907 Fragments arranged by size: 2907 1730 1307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3078 3299 844 Size: 2234 221 3489 Fragments arranged by size: 3489 2234 221 BsrBI CCG'CTC Cuts at: 1394 1823 2881 3831 1394 Size: 429 1058 950 3507 Fragments arranged by size: 3507 1058 950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4439 4613 65 Size: 1183 3191 174 1396 Fragments arranged by size: 3191 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2280 2925 4588 4775 1700 Size: 110 470 645 1663 187 2869 Fragments arranged by size: 2869 1663 645 470 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 2876 4004 5016 2876 Size: 1128 1012 3804 Fragments arranged by size: 3804 1128 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5944 BstBI TT'CG_AA Cuts at: 4748 4748 Size: 5944 BstEII G'GTnAC_C Cuts at: 1184 2380 2884 1184 Size: 1196 504 4244 Fragments arranged by size: 4244 1196 504 BstYI r'GATC_y Cuts at: 1972 3274 3641 4026 4043 5495 5581 5593 5939 Size: 1302 367 385 17 1452 86 12 346 Cuts at: 5939 1972 Size: 1977 Fragments arranged by size: 1977 1452 1302 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2259 2259 Size: 5944 BtgI C'CryG_G Cuts at: 513 1065 2475 513 Size: 552 1410 3982 Fragments arranged by size: 3982 1410 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5205 Fragments arranged by size: 5205 683 56 BtsI GCAGTG_nn' Cuts at: 3460 4254 4274 3460 Size: 794 20 5130 Fragments arranged by size: 5130 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5909 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2117 4951 809 Size: 507 801 2834 1802 Fragments arranged by size: 2834 1802 801 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2057 2697 Size: 54 1425 50 275 18 198 29 640 Cuts at: 2697 3003 3391 4280 5909 8 Size: 306 388 889 1629 43 Fragments arranged by size: 1629 1425 889 640 388 306 275 198 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2841 3874 1353 Size: 1488 1033 3423 Fragments arranged by size: 3423 1488 1033 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4531 4905 5051 387 Size: 1064 138 2942 374 146 1280 Fragments arranged by size: 2942 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2839 2865 2878 3143 4007 4604 5391 Size: 393 1207 26 13 265 864 597 787 Cuts at: 5391 5659 1239 Size: 268 1524 Fragments arranged by size: 1524 1207 864 787 597 393 268 265 26 13 EcoO109I rG'GnC_Cy Cuts at: 2770 2771 3114 2770 Size: 1 343 5600 Fragments arranged by size: 5600 343 1 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FspI TGC'GCA Cuts at: 2762 4450 2762 Size: 1688 4256 FspAI rTGC'GCAy Cuts at: 2762 2762 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 2666 5091 1678 Size: 988 2425 2531 Fragments arranged by size: 2531 2425 988 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2568 2600 4585 4617 4659 Size: 32 135 32 996 32 1985 32 42 Cuts at: 4659 4691 1373 Size: 32 2626 Fragments arranged by size: 2626 1985 996 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3288 3534 669 Size: 988 281 1350 246 3079 Fragments arranged by size: 3079 1350 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5944 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 3543 3543 Size: 5944 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5058 5242 1305 Size: 3753 184 2007 Fragments arranged by size: 3753 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 10 Size: 54 1425 343 198 3924 Fragments arranged by size: 3924 1425 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 3236 3902 4261 4420 518 Size: 648 2070 666 359 159 2042 Fragments arranged by size: 2070 2042 666 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2252 3187 3360 4037 5185 5430 1606 Size: 646 935 173 677 1148 245 2120 Fragments arranged by size: 2120 1148 935 677 646 245 173 NcoI C'CATG_G Cuts at: 513 1065 2475 513 Size: 552 1410 3982 Fragments arranged by size: 3982 1410 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 2185 3302 4847 1132 Size: 97 956 1117 1545 2229 Fragments arranged by size: 2229 1545 1117 956 97 PasI CC'CwG_GG Cuts at: 1348 2776 1348 Size: 1428 4516 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5944 PfoI T'CCnGG_A Cuts at: 3027 3210 3027 Size: 183 5761 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PmlI CAC'GTG Cuts at: 2402 2402 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2534 2566 4013 4045 5526 5558 2534 Size: 32 1447 32 1481 32 2920 Fragments arranged by size: 2920 1481 1447 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3114 3114 Size: 5944 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5944 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PspOMI G'GGCC_C Cuts at: 2770 2770 Size: 5944 PstI C_TGCA'G Cuts at: 830 2095 2364 3296 830 Size: 1265 269 932 3478 Fragments arranged by size: 3478 1265 932 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 PvuII CAG'CTG Cuts at: 2252 2252 Size: 5944 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 4192 5904 4192 Size: 1712 4232 SfcI C'TryA_G Cuts at: 826 1045 2091 2360 2817 3292 4427 5108 5299 Size: 219 1046 269 457 475 1135 681 191 Cuts at: 5299 826 Size: 1471 Fragments arranged by size: 1471 1135 1046 681 475 457 269 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2056 2056 Size: 5944 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 3271 1872 Size: 1399 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 2951 4048 4949 5211 820 Size: 197 926 723 285 1097 901 262 1553 Fragments arranged by size: 1553 1097 926 901 723 285 262 197 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 2307 1517 Size: 790 5154 StyI C'CwwG_G Cuts at: 513 1065 2031 2475 3383 513 Size: 552 966 444 908 3074 Fragments arranged by size: 3074 966 908 552 444 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3069 3971 3988 4141 4326 4771 325 Size: 1255 1489 902 17 153 185 445 1498 Fragments arranged by size: 1498 1489 1255 902 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2739 4190 5902 Size: 274 80 33 51 1443 762 1451 1712 Cuts at: 5902 96 Size: 138 Fragments arranged by size: 1712 1451 1443 762 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5904 5936 5904 Size: 32 5912 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5944 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2149 Size: 5944 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 3269 1870 Size: 1399 4545 XmnI GAAnn'nnTTC Cuts at: 4073 4073 Size: 5944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3752 276 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI