Restriction map for
pFN21AA0929
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# NO CUT ENZYMES ( 45 enzymes) #
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Acc65I AflII AhdI AscI AseI
AsnI Asp718I AspEI AviII BbrPI
BfrI BsiWI Bsp1407I BsrGI BssHII
Bst1107I BstZ17I CpoI EagI Eam1105I
EclXI Eco52I EcoT22I FseI FspI
HpaI KpnI MfeI MluI MunI
NdeI NotI NsiI PacI PmaCI
PmeI PmlI PshBI RsrII SalI
SfiI SnaBI SspBI SspI SwaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 2072 GACGTC GACGT^C
AccIII 2271 TCCGGA T^CCGGA
AclI 10714 AACGTT AA^CGTT
Asp700I 9220 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 9680 GTATCC GTATCC(6/5)
BclI 3237 TGATCA T^GATCA
BlpI 5224 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5224 GCTGAGC GC^T[ACGT]AGC
BsaBI 3691 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2271 TCCGGA T^CCGGA
BspDI 3364 ATCGAT AT^CGAT
BspEI 2271 TCCGGA T^CCGGA
BspLU11I 9480 ACATGT A^CATGT
CelII 5224 GCTGAGC GC^T[ACGT]AGC
ClaI 3364 ATCGAT AT^CGAT
DrdI 10201 GACCTTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3237 TGATCA T^GATCA
KspI 9512 CCGCGG CCGC^GG
MamI 3691 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2271 TCCGGA T^CCGGA
NruI 492 TCGCGA TCG^CGA
PflMI 10394 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 10714 AACGTT AA^CGTT
PvuI 8259 CGATCG CGAT^CG
SacII 9512 CCGCGG CCGC^GG
ScaI 9007 AGTACT AGT^ACT
SexAI 3999 ACCTGGT A^CC[AT]GGT
SpeI 9408 ACTAGT A^CTAGT
Van91I 10394 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 9220 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 4975 GTAGAC GT^[AC][GT]AC
7410 GTCTAC
AgeI 7191 ACCGGT A^CCGGT
9794 ACCGGT
Alw44I 6632 GTGCAC G^TGCAC
9763 GTGCAC
ApaLI 6632 GTGCAC G^TGCAC
9763 GTGCAC
AvrII 484 CCTAGG C^CTAGG
3712 CCTAGG
BalI 3845 TGGCCA TGG^CCA
8516 TGGCCA
BamHI 3696 GGATCC G^GATCC
7340 GGATCC
BcgI 1480 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3356 GCATTGACATCG
BlnI 484 CCTAGG C^CTAGG
3712 CCTAGG
BsaAI 4121 TACGTG [CT]AC^GT[GA]
10747 TACGTG
BsiEI 5821 CGGTCG CG[GA][CT]^CG
8259 CGATCG
BspHI 465 TCATGA T^CATGA
10847 TCATGA
BssSI 3502 CACGAG CACGAG(-5/-1)
4247 CTCGTG
DraI 2853 TTTAAA TTT^AAA
3795 TTTAAA
DraIII 6805 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
9278 CACAGCGTG
EcoRV 529 GATATC GAT^ATC
3583 GATATC
MluNI 3845 TGGCCA TGG^CCA
8516 TGGCCA
MscI 3845 TGGCCA TGG^CCA
8516 TGGCCA
NheI 652 GCTAGC G^CTAGC
1336 GCTAGC
PinAI 7191 ACCGGT A^CCGGT
9794 ACCGGT
RcaI 465 TCATGA T^CATGA
10847 TCATGA
SacI 4642 GAGCTC GAGCT^C
5952 GAGCTC
SapI 4429 GAAGAGC GCTCTTC(1/4)
7426 GCTCTTC
SbfI 8497 CCTGCAGG CCTGCA^GG
10683 CCTGCAGG
SgrAI 5746 CGCCGGCG C[GA]^CCGG[CT]G
7190 CACCGGTG
SphI 1085 GCATGC GCATG^C
1415 GCATGC
Sse8387I 8497 CCTGCAGG CCTGCA^GG
10683 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI AspHI AspI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstBI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI NspV PaeR7I PflFI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SfuI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI