Restriction map for
pFN21AA0929
(Circular) (Six-base) MAPSORT of: pFN21AA0929.seq Check: 4509 from: 1 to: 15574
pFN21AA0929 15574 bp
With 182 enzymes: SgfI *
November 17, 2015 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5147 8624 5147
Size: 3477 12097
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 13384 278
Size: 53 83 186 936 2545 9303 2468
Fragments arranged by size:
9303 2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6981 9416 12917 1937
Size: 5044 2435 3501 4594
Fragments arranged by size:
5044 4594 3501 2435
Acc65I G'GTAC_C
Cuts at: 12895 12895
Size: 15574
AclI AA'CG_TT
Cuts at: 1563 12720 13701 14074 1563
Size: 11157 981 373 3063
Fragments arranged by size:
11157 3063 981 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2274 2964 2985 4371 4616 5367 5901 7308
Size: 642 690 21 1386 245 751 534 1407
Cuts at: 7308 7679 10335 10784 11940 12407 12525 13637 15021
Size: 371 2656 449 1156 467 118 1112 1384
Cuts at: 15021 15289 1632
Size: 268 1917
Fragments arranged by size:
2656 1917 1407 1386 1384 1156 1112 751
690 642 534 467 449 371 268 245
118 21
AfeI AGC'GCT
Cuts at: 3260 4232 11229 3260
Size: 972 6997 7605
Fragments arranged by size:
7605 6997 972
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 15377
AflIII A'CryG_T
Cuts at: 1203 4944 7907 8337 11485 14473 15450 1203
Size: 3741 2963 430 3148 2988 977 1327
Fragments arranged by size:
3741 3148 2988 2963 1327 977 430
AgeI A'CCGG_T
Cuts at: 9196 11799 14405 9196
Size: 2603 2606 10365
Fragments arranged by size:
10365 2606 2603
AhdI GACnn_n'nnGTC
Cuts at: 14303 14303
Size: 15574
AleI CACnn'nnGTG
Cuts at: 1166 4565 7928 10010 1166
Size: 3399 3363 2082 6730
Fragments arranged by size:
6730 3399 3363 2082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 11087 12001 12050 1184
Size: 9903 914 49 4708
Fragments arranged by size:
9903 4708 914 49
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2208 2240 4258 4290 5644 5676 2208
Size: 32 2018 32 1354 32 12106
Fragments arranged by size:
12106 2018 1354 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2653 2828 6746 7139 7229 7286 7316 8647 9563
Size: 175 3918 393 90 57 30 1331 916
Cuts at: 9563 11396 11722 12415 12443 13317 14889 2653
Size: 1833 326 693 28 874 1572 3338
Fragments arranged by size:
3918 3338 1833 1572 1331 916 874 693
393 326 175 90 57 30 28
ApaI G_GGCC'C
Cuts at: 7937 9948 10896 11281 11912 12516 7937
Size: 2011 948 385 631 604 10995
Fragments arranged by size:
10995 2011 948 631 604 385
ApaLI G'TGCA_C
Cuts at: 8637 11768 13631 14787 8637
Size: 3131 1863 1156 9424
Fragments arranged by size:
9424 3131 1863 1156
ApoI r'AATT_y
Cuts at: 1493 2018 2954 2963 3143 3173 5010 5804 5875
Size: 525 936 9 180 30 1837 794 71
Cuts at: 5875 6170 6298 9109 9778 10418 12311 12883 13109
Size: 295 128 2811 669 640 1893 572 226
Cuts at: 13109 13261 15370 1493
Size: 152 2109 1697
Fragments arranged by size:
2811 2109 1893 1837 1697 936 794 669
640 572 525 295 226 180 152 128
71 30 9
AseI AT'TA_AT
Cuts at: 160 1032 14128 160
Size: 872 13096 1606
Fragments arranged by size:
13096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15574
AvaI C'yCGr_G
Cuts at: 1870 1943 2400 2556 3837 3912 4524 7094 7455
Size: 73 457 156 1281 75 612 2570 361
Cuts at: 7455 10616 10888 10973 11241 11348 11382 12899 1870
Size: 3161 272 85 268 107 34 1517 4545
Fragments arranged by size:
4545 3161 2570 1517 1281 612 457 361
272 268 156 107 85 75 73 34
AvrII C'CTAG_G
Cuts at: 2489 5717 2489
Size: 3228 12346
BamHI G'GATC_C
Cuts at: 5701 9345 12904 13271 5701
Size: 3644 3559 367 8004
Fragments arranged by size:
8004 3644 3559 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6013 6874 7581 8874 11536
Size: 325 217 627 4226 861 707 1293 2662
Cuts at: 11536 12895 14350 618
Size: 1359 1455 1842
Fragments arranged by size:
4226 2662 1842 1455 1359 1293 861 707
627 325 217
BanII G_rGCy'C
Cuts at: 1391 2427 2497 2874 6651 7650 7937 7961 9362
Size: 1036 70 377 3777 999 287 24 1401
Cuts at: 9362 9412 9905 9948 10286 10623 10896 11248 11281
Size: 50 493 43 338 337 273 352 33
Cuts at: 11281 11912 11931 12516 12893 1391
Size: 631 19 585 377 4072
Fragments arranged by size:
4072 3777 1401 1036 999 631 585 493
377 377 352 338 337 287 273 70
50 43 33 24 19
BbeI G_GCGC'C
Cuts at: 6878 7585 8878 6878
Size: 707 1293 13574
Fragments arranged by size:
13574 1293 707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4377 5268 5279 6123 6854 6902 8340
Size: 971 2478 891 11 844 731 48 1438
Cuts at: 8340 10355 928
Size: 2015 6147
Fragments arranged by size:
6147 2478 2015 1438 971 891 844 731
48 11
BbvCI CC'TCA_GC
Cuts at: 4970 7980 8427 8755 11559 11943 4970
Size: 3010 447 328 2804 384 8601
Fragments arranged by size:
8601 3010 2804 447 384 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3474 3508 5350 5384 13765 13799 1903
Size: 34 1537 34 1842 34 8381 34 3678
Fragments arranged by size:
8381 3678 1842 1537 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 11696 13466 14676 11696
Size: 1770 1210 12594
Fragments arranged by size:
12594 1770 1210
BclI T'GATC_A
Cuts at: 1548 5242 1548
Size: 3694 11880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8453 11552 12701
Size: 107 122 71 631 605 6781 3099 1149
Cuts at: 12701 14185 136
Size: 1484 1525
Fragments arranged by size:
6781 3099 1525 1484 1149 631 605 122
107 71
BglII A'GATC_T
Cuts at: 5995 8117 8617 12762 15569 5995
Size: 2122 500 4145 2807 6000
Fragments arranged by size:
6000 4145 2807 2122 500
BlpI GC'TnA_GC
Cuts at: 7230 12991 7230
Size: 5761 9813
Bme1580I G_kGCm'C
Cuts at: 1790 7937 8641 9165 9948 10031 10896 11076 11281
Size: 6147 704 524 783 83 865 180 205
Cuts at: 11281 11772 11912 12148 12516 13635 14791 1790
Size: 491 140 236 368 1119 1156 2573
Fragments arranged by size:
6147 2573 1156 1119 865 783 704 524
491 368 236 205 180 140 83
BmgBI CAC'GTC
Cuts at: 1318 4945 7910 8338 1318
Size: 3627 2965 428 8554
Fragments arranged by size:
8554 3627 2965 428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2511 2620 2836
Size: 416 53 83 399 24 1094 109 216
Cuts at: 2836 5153 5661 8281 8365 11063 11071 11193 11697
Size: 2317 508 2620 84 2698 8 122 504
Cuts at: 11697 13305 14263 442
Size: 1608 958 1753
Fragments arranged by size:
2698 2620 2317 1753 1608 1094 958 508
504 416 399 216 122 109 84 83
53 24 8
BmtI G_CTAG'C
Cuts at: 1056 2661 3345 1056
Size: 1605 684 13285
Fragments arranged by size:
13285 1605 684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2701 2781 3780 4113 4835 7293 8113 8414
Size: 1462 80 999 333 722 2458 820 301
Cuts at: 8414 8622 8718 9411 9457 9630 9699 9993 11071
Size: 208 96 693 46 173 69 294 1078
Cuts at: 11071 11699 11758 12566 14234 1239
Size: 628 59 808 1668 2579
Fragments arranged by size:
2579 2458 1668 1462 1078 999 820 808
722 693 628 333 301 294 208 173
96 80 69 59 46
Bpu10I CC'TnA_GC
Cuts at: 4779 4970 7980 8427 8755 9086 10285 11150 11559
Size: 191 3010 447 328 331 1199 865 409
Cuts at: 11559 11904 11943 12120 4779
Size: 345 39 177 8233
Fragments arranged by size:
8233 3010 1199 865 447 409 345 331
328 191 177 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2024 3383 4757 5098 5511 7421 7790 8952 9198
Size: 1359 1374 341 413 1910 369 1162 246
Cuts at: 9198 10479 12024 12410 12650 13699 14564 14862 2024
Size: 1281 1545 386 240 1049 865 298 2736
Fragments arranged by size:
2736 1910 1545 1374 1359 1281 1162 1049
865 413 386 369 341 298 246 240
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3587 4020 4642 5745 10781 12355 14237
Size: 617 2088 433 622 1103 5036 1574 1882
Cuts at: 14237 882
Size: 2219
Fragments arranged by size:
5036 2219 2088 1882 1574 1103 622 617
433
BsaAI yAC'GTr
Cuts at: 493 1204 6128 12754 13365 493
Size: 711 4924 6626 611 2702
Fragments arranged by size:
6626 4924 2702 711 611
BsaBI GATnn'nnATC
Cuts at: 5700 15568 5700
Size: 9868 5706
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4078 6875 7215 7582
Size: 53 83 186 936 2545 2797 340 367
Cuts at: 7582 8875 13381 13763 14398 275
Size: 1293 4506 382 635 1451
Fragments arranged by size:
4506 2797 2545 1451 1293 936 635 382
367 340 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 4276 9196 11799 14007 14405 14679 14826 15237
Size: 112 4920 2603 2208 398 274 147 411
Cuts at: 15237 4164
Size: 4501
Fragments arranged by size:
4920 4501 2603 2208 411 398 274 147
112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2434 2464 3258 3288 9409
Size: 30 165 30 1031 30 794 30 6121
Cuts at: 9409 9439 9455 9485 9628 9658 10650 10680 12719
Size: 30 16 30 143 30 992 30 2039
Cuts at: 12719 12749 1178
Size: 30 4003
Fragments arranged by size:
6121 4003 2039 1031 992 794 165 143
30 30 30 30 30 30 30 30
30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2634 4051 4153 5538 7443 7784 7787 7997
Size: 1448 1417 102 1385 1905 341 3 210
Cuts at: 7997 8000 9051 9054 9221 9413 9801 10947 11229
Size: 3 1051 3 167 192 388 1146 282
Cuts at: 11229 11366 11904 11931 1186
Size: 137 538 27 4829
Fragments arranged by size:
4829 1905 1448 1417 1385 1146 1051 538
388 341 282 210 192 167 137 102
27 3 3 3
BseYI C'CCAG_C
Cuts at: 3403 5120 7149 7279 8581 9186 9266 9941 10853
Size: 1717 2029 130 1302 605 80 675 912
Cuts at: 10853 10895 10903 11006 11259 11311 11337 11835 11970
Size: 42 8 103 253 52 26 498 135
Cuts at: 11970 11997 12094 12275 14777 15273 3403
Size: 27 97 181 2502 496 3704
Fragments arranged by size:
3704 2502 2029 1717 1302 912 675 605
498 496 253 181 135 130 103 97
80 52 42 27 26 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2814 3005 5578 8484 10092 10113 11310
Size: 573 1078 191 2573 2906 1608 21 1197
Cuts at: 11310 11420 11423 11780 11961 11972 12467 1163
Size: 110 3 357 181 11 495 4270
Fragments arranged by size:
4270 2906 2573 1608 1197 1078 573 495
357 191 181 110 21 11 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7829 10267 13024 13785 14813 1540
Size: 275 186 5828 2438 2757 761 1028 2301
Fragments arranged by size:
5828 2757 2438 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6651 7961 8641 9935 10329 11772 13635 13720 14791
Size: 1310 680 1294 394 1443 1863 85 1071
Cuts at: 14791 6651
Size: 7434
Fragments arranged by size:
7434 1863 1443 1310 1294 1071 680 394
85
BsmI GAATG_Cn'
Cuts at: 9232 9883 10042 10084 13085 13178 9232
Size: 651 159 42 3001 93 11628
Fragments arranged by size:
11628 3001 651 159 93 42
BsmBI CGTCTCn'nnnn_
Cuts at: 4104 4291 6922 9865 12466 4104
Size: 187 2631 2943 2601 7212
Fragments arranged by size:
7212 2943 2631 2601 187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 2497 2874 6651 7650 7937 7961
Size: 399 637 70 377 3777 999 287 24
Cuts at: 7961 8641 9165 9362 9412 9905 9935 9948 10031
Size: 680 524 197 50 493 30 13 83
Cuts at: 10031 10286 10329 10623 10896 11076 11248 11281 11772
Size: 255 43 294 273 180 172 33 491
Cuts at: 11772 11912 11931 12148 12516 12893 13635 13720 14791
Size: 140 19 217 368 377 742 85 1071
Cuts at: 14791 1391
Size: 2174
Fragments arranged by size:
3777 2174 1071 999 742 680 637 524
493 491 399 377 377 368 294 287
273 255 217 197 180 172 140 85
83 70 50 43 33 30 24 19
13
BspEI T'CCGG_A
Cuts at: 4276 15237 4276
Size: 10961 4613
BspHI T'CATG_A
Cuts at: 2470 12852 13463 15193 2470
Size: 10382 611 1730 2851
Fragments arranged by size:
10382 2851 1730 611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5147 5221 6955 6993 7801 8624 12112 12696
Size: 4303 74 1734 38 808 823 3488 584
Cuts at: 12696 12929 844
Size: 233 3489
Fragments arranged by size:
4303 3489 3488 1734 823 808 584 233
74 38
BsrBI CCG'CTC
Cuts at: 1394 1823 2407 2584 7632 13461 1394
Size: 429 584 177 5048 5829 3507
Fragments arranged by size:
5829 5048 3507 584 429 177
BsrDI GCAATG_nn'
Cuts at: 65 1248 9552 9720 10413 12861 14069 14243 65
Size: 1183 8304 168 693 2448 1208 174 1396
Fragments arranged by size:
8304 2448 1396 1208 1183 693 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 7752 8080 8759 9196 10804 11799 14218
Size: 110 5942 328 679 437 1608 995 2419
Cuts at: 14218 14405 1700
Size: 187 2869
Fragments arranged by size:
5942 2869 2419 1608 995 679 437 328
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 15574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 15574
BssSI C'ACGA_G
Cuts at: 5507 6252 13634 14646 5507
Size: 745 7382 1012 6435
Fragments arranged by size:
7382 6435 1012 745
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2828 6935 10079 10181 10511 11767 12415 794
Size: 2034 4107 3144 102 330 1256 648 3953
Fragments arranged by size:
4107 3953 3144 2034 1256 648 330 102
BstBI TT'CG_AA
Cuts at: 3458 3790 5977 6304 14378 3458
Size: 332 2187 327 8074 4654
Fragments arranged by size:
8074 4654 2187 332 327
BstEII G'GTnAC_C
Cuts at: 1184 2610 7055 7616 9256 11746 1184
Size: 1426 4445 561 1640 2490 5012
Fragments arranged by size:
5012 4445 2490 1640 1426 561
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3263 7930 8386 10408 2011
Size: 1252 4667 456 2022 7177
Fragments arranged by size:
7177 4667 2022 1252 456
BstYI r'GATC_y
Cuts at: 1972 2395 2711 4810 5701 5995 6558 7129 8117
Size: 423 316 2099 891 294 563 571 988
Cuts at: 8117 8617 9345 10389 12762 12904 13271 13656 13673
Size: 500 728 1044 2373 142 367 385 17
Cuts at: 13673 15125 15211 15223 15569 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2373 2099 1977 1452 1044 988 891 728
571 563 500 423 385 367 346 316
294 142 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3810 7815 7914 9482 3810
Size: 4005 99 1568 9902
Fragments arranged by size:
9902 4005 1568 99
BtgI C'CryG_G
Cuts at: 513 1065 7611 7962 8403 9404 10020 11456 11517
Size: 552 6546 351 441 1001 616 1436 61
Cuts at: 11517 11752 12366 12870 513
Size: 235 614 504 3217
Fragments arranged by size:
6546 3217 1436 1001 616 614 552 504
441 351 235 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 8398 10455 487
Size: 683 56 7172 2057 5606
Fragments arranged by size:
7172 5606 2057 683 56
BtsI GCAGTG_nn'
Cuts at: 2510 2761 2770 3670 7218 7383 8145 8803 9293
Size: 251 9 900 3548 165 762 658 490
Cuts at: 9293 10080 10101 10182 13090 13884 13904 2510
Size: 787 21 81 2908 794 20 4180
Fragments arranged by size:
4180 3548 2908 900 794 787 762 658
490 251 165 81 21 20 9
ClaI AT'CG_AT
Cuts at: 1554 5370 1554
Size: 3816 11758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 9535 9570 529
Size: 35 8971 35 6533
Fragments arranged by size:
8971 6533 35 35
DraI TTT'AAA
Cuts at: 4860 5802 12878 4860
Size: 942 7076 7556
Fragments arranged by size:
7556 7076 942
DraIII CAC_nnn'GTG
Cuts at: 8815 11288 8815
Size: 2473 13101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 12212 14581 809
Size: 507 10896 2369 1802
Fragments arranged by size:
10896 2369 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5850 8532 10143
Size: 54 1425 50 275 18 4020 2682 1611
Cuts at: 10143 10398 10521 10806 11219 12013 12625 13021 13910
Size: 255 123 285 413 794 612 396 889
Cuts at: 13910 15539 8
Size: 1629 43
Fragments arranged by size:
4020 2682 1629 1611 1425 889 794 612
413 396 285 275 255 123 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 13021 1537
Size: 275 11209 4090
Fragments arranged by size:
11209 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3000 3724 5787 6250 6429 6885 7403 7761
Size: 1647 724 2063 463 179 456 518 358
Cuts at: 7761 9438 12225 13504 1353
Size: 1677 2787 1279 3423
Fragments arranged by size:
3423 2787 2063 1677 1647 1279 724 518
463 456 358 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 14161 14535 14681 387
Size: 1064 138 12572 374 146 1280
Fragments arranged by size:
12572 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6649 7959 6649
Size: 1310 14264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2274 2701 2781 2964 2985 3780 4113
Size: 393 642 427 80 183 21 795 333
Cuts at: 4113 4371 4616 4835 5367 5901 7293 7308 7679
Size: 258 245 219 532 534 1392 15 371
Cuts at: 7679 8113 8414 8622 8718 9411 9457 9630 9699
Size: 434 301 208 96 693 46 173 69
Cuts at: 9699 9993 10335 10784 11071 11699 11758 11940 12407
Size: 294 342 449 287 628 59 182 467
Cuts at: 12407 12525 12566 13637 14234 15021 15289 1239
Size: 118 41 1071 597 787 268 1524
Fragments arranged by size:
1524 1392 1071 795 787 693 642 628
597 534 532 467 449 434 427 393
371 342 333 301 294 287 268 258
245 219 208 183 182 173 118 96
80 69 59 46 41 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 9587 11162 11919 9587
Size: 1575 757 13242
Fragments arranged by size:
13242 1575 757
EcoO109I rG'GnC_Cy
Cuts at: 2682 7098 7836 7934 8470 9592 9890 9965 10490
Size: 4416 738 98 536 1122 298 75 525
Cuts at: 10490 10795 10892 11277 11278 11554 11908 11909 12063
Size: 305 97 385 1 276 354 1 154
Cuts at: 12063 12412 12512 12513 2682
Size: 349 100 1 5743
Fragments arranged by size:
5743 4416 1122 738 536 525 385 354
349 305 298 276 154 100 98 97
75 1 1 1
EcoRI G'AATT_C
Cuts at: 5875 9109 12311 12883 13261 5875
Size: 3234 3202 572 378 8188
Fragments arranged by size:
8188 3234 3202 572 378
EcoRV GAT'ATC
Cuts at: 2536 5590 2536
Size: 3054 12520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2960 3095 4604 4769 5030 6239 7533 8870 9546
Size: 135 1509 165 261 1209 1294 1337 676
Cuts at: 9546 9811 12691 2960
Size: 265 2880 5843
Fragments arranged by size:
5843 2880 1509 1337 1294 1209 676 265
261 165 135
FspI TGC'GCA
Cuts at: 14080 14080
Size: 15574
HaeII r_GCGC'y
Cuts at: 1678 3262 4234 4843 6878 7585 8648 8878 10377
Size: 1584 972 609 2035 707 1063 230 1499
Cuts at: 10377 11030 11231 14721 1678
Size: 653 201 3490 2531
Fragments arranged by size:
3490 2531 2035 1584 1499 1063 972 707
653 609 230 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 2434 2466 3001
Size: 32 135 32 674 32 156 32 535
Cuts at: 3001 3033 4934 4966 5484 5516 6269 6301 8204
Size: 32 1901 32 518 32 753 32 1903
Cuts at: 8204 8236 8264 8296 8672 8704 9098 9130 9455
Size: 32 28 32 376 32 394 32 325
Cuts at: 9455 9487 10249 10268 10281 10300 10586 10618 11887
Size: 32 762 19 13 19 286 32 1269
Cuts at: 11887 11919 14215 14247 14289 14321 1373
Size: 32 2296 32 42 32 2626
Fragments arranged by size:
2626 2296 1903 1901 1269 762 753 674
535 518 394 376 325 286 156 135
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 28 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 6331 9463 9610 10191 10876 12918
Size: 988 281 4393 3132 147 581 685 2042
Cuts at: 12918 13164 669
Size: 246 3079
Fragments arranged by size:
4393 3132 3079 2042 988 685 581 281
246 147
HindIII A'AGCT_T
Cuts at: 748 4586 6216 7472 10757 748
Size: 3838 1630 1256 3285 5565
Fragments arranged by size:
5565 3838 3285 1630 1256
HpaI GTT'AAC
Cuts at: 13164 13164
Size: 15574
KasI G'GCGC_C
Cuts at: 6874 7581 8874 6874
Size: 707 1293 13574
Fragments arranged by size:
13574 1293 707
KpnI G_GTAC'C
Cuts at: 12899 12899
Size: 15574
MfeI C'AATT_G
Cuts at: 13173 13173
Size: 15574
MluI A'CGCG_T
Cuts at: 15450 15450
Size: 15574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5698 7112 8982 9170 12858 14688 14872 1305
Size: 4393 1414 1870 188 3688 1830 184 2007
Fragments arranged by size:
4393 3688 2007 1870 1830 1414 188 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5852 10523 10
Size: 54 1425 343 4020 4671 5061
Fragments arranged by size:
5061 4671 4020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4565 5694 6656 7928 10010 10253
Size: 648 843 2556 1129 962 1272 2082 243
Cuts at: 10253 11048 11069 11484 13532 13891 14050 518
Size: 795 21 415 2048 359 159 2042
Fragments arranged by size:
2556 2082 2048 2042 1272 1129 962 843
795 648 415 359 243 159 21
MspA1I CmG'CkG
Cuts at: 1606 2697 3584 3658 5227 6970 7373 7709 7853
Size: 1091 887 74 1569 1743 403 336 144
Cuts at: 7853 10064 10109 10366 10720 10934 11251 11315 11519
Size: 2211 45 257 354 214 317 64 204
Cuts at: 11519 11778 11900 12098 12440 12559 12990 13667 14815
Size: 259 122 198 342 119 431 677 1148
Cuts at: 14815 15060 1606
Size: 245 2120
Fragments arranged by size:
2211 2120 1743 1569 1148 1091 887 677
431 403 354 342 336 317 259 257
245 214 204 198 144 122 119 74
64 45
NaeI GCC'GGC
Cuts at: 7754 8761 10806 7754
Size: 1007 2045 12522
Fragments arranged by size:
12522 2045 1007
NarI GG'CG_CC
Cuts at: 6875 7582 8875 6875
Size: 707 1293 13574
Fragments arranged by size:
13574 1293 707
NcoI C'CATG_G
Cuts at: 513 1065 7611 7962 11752 513
Size: 552 6546 351 3790 4335
Fragments arranged by size:
6546 4335 3790 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 15574
NgoMIV G'CCGG_C
Cuts at: 7752 8759 10804 7752
Size: 1007 2045 12522
Fragments arranged by size:
12522 2045 1007
NheI G'CTAG_C
Cuts at: 1052 2657 3341 1052
Size: 1605 684 13285
Fragments arranged by size:
13285 1605 684
NotI GC'GGCC_GC
Cuts at: 13021 13021
Size: 15574
NruI TCG'CGA
Cuts at: 2499 2499
Size: 15574
NspI r_CATG'y
Cuts at: 1132 1229 3094 3424 11489 12932 14477 1132
Size: 97 1865 330 8065 1443 1545 2229
Fragments arranged by size:
8065 2229 1865 1545 1443 330 97
PasI CC'CwG_GG
Cuts at: 1348 8187 10241 10654 10681 11164 11511 1348
Size: 6839 2054 413 27 483 347 5411
Fragments arranged by size:
6839 5411 2054 483 413 347 27
PciI A'CATG_T
Cuts at: 11485 14473 11485
Size: 2988 12586
PflMI CCAn_nnn'nTGG
Cuts at: 1108 12405 1108
Size: 11297 4277
PfoI T'CCnGG_A
Cuts at: 4090 8599 8697 11885 4090
Size: 4509 98 3188 7779
Fragments arranged by size:
7779 4509 3188 98
PmeI GTTT'AAAC
Cuts at: 12878 12878
Size: 15574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 13643 13675 15156 15188 13643
Size: 32 1481 32 14029
Fragments arranged by size:
14029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2682 7836 10795 12063 2682
Size: 5154 2959 1268 6193
Fragments arranged by size:
6193 5154 2959 1268
PshAI GACnn'nnGTC
Cuts at: 1613 10266 10793 11495 1613
Size: 8653 527 702 5692
Fragments arranged by size:
8653 5692 702 527
PsiI TTA'TAA
Cuts at: 13144 13144
Size: 15574
PspOMI G'GGCC_C
Cuts at: 7933 9944 10892 11277 11908 12512 7933
Size: 2011 948 385 631 604 10995
Fragments arranged by size:
10995 2011 948 631 604 385
PspXI vC'TCGA_Gb
Cuts at: 3837 4524 11382 3837
Size: 687 6858 8029
Fragments arranged by size:
8029 6858 687
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3288 3320 6158 6190 3288
Size: 32 2838 32 12672
Fragments arranged by size:
12672 2838 32 32
PstI C_TGCA'G
Cuts at: 830 2821 4792 5232 7225 7378 7537 7708 7798
Size: 1991 1971 440 1993 153 159 171 90
Cuts at: 7798 7852 8200 8652 9313 10507 11062 11256 11937
Size: 54 348 452 661 1194 555 194 681
Cuts at: 11937 12693 12762 12926 830
Size: 756 69 164 3478
Fragments arranged by size:
3478 1993 1991 1971 1194 756 681 661
555 452 440 348 194 171 164 159
153 90 69 54
PvuI CG_AT'CG
Cuts at: 2001 10267 2001
Size: 8266 7308
PvuII CAG'CTG
Cuts at: 3584 3658 5227 6970 7373 7853 10720 10934 11315
Size: 74 1569 1743 403 480 2867 214 381
Cuts at: 11315 11900 12098 12440 12559 3584
Size: 585 198 342 119 6599
Fragments arranged by size:
6599 2867 1743 1569 585 480 403 381
342 214 198 119 74
SacI G_AGCT'C
Cuts at: 6651 7961 6651
Size: 1310 14264
SacII CC_GC'GG
Cuts at: 11520 11520
Size: 15574
SalI G'TCGA_C
Cuts at: 1936 12916 1936
Size: 10980 4594
SanDI GG'GwC_CC
Cuts at: 12063 12063
Size: 15574
SapI GCTCTTCn'nnn_
Cuts at: 6429 9438 6429
Size: 3009 12565
SbfI CC_TGCA'GG
Cuts at: 10507 12693 12926 10507
Size: 2186 233 13155
Fragments arranged by size:
13155 2186 233
ScaI AGT'ACT
Cuts at: 11014 13822 15534 11014
Size: 2808 1712 11054
Fragments arranged by size:
11054 2808 1712
SexAI A'CCwGG_T
Cuts at: 6004 6004
Size: 15574
SfcI C'TryA_G
Cuts at: 826 1045 2389 2539 2817 2919 4177 4788 5228
Size: 219 1344 150 278 102 1258 611 440
Cuts at: 5228 7221 7374 7533 7704 7794 7848 8196 8648
Size: 1993 153 159 171 90 54 348 452
Cuts at: 8648 8715 8934 9309 10503 10866 11058 11209 11252
Size: 67 219 375 1194 363 192 151 43
Cuts at: 11252 11933 12689 12758 12922 14057 14738 14929 826
Size: 681 756 69 164 1135 681 191 1471
Fragments arranged by size:
1993 1471 1344 1258 1194 1135 756 681
681 611 452 440 375 363 348 278
219 219 192 191 171 164 159 153
151 150 102 90 69 67 54 43
SfoI GGC'GCC
Cuts at: 6876 7583 8876 6876
Size: 707 1293 13574
Fragments arranged by size:
13574 1293 707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15574
SgrAI Cr'CCGG_yG
Cuts at: 1700 7752 9196 1700
Size: 6052 1444 8078
Fragments arranged by size:
8078 6052 1444
SmaI CCC'GGG
Cuts at: 1872 2558 7096 10975 12901 1872
Size: 686 4538 3879 1926 4545
Fragments arranged by size:
4545 4538 3879 1926 686
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2039 2400 3398 3837 4524 4772
Size: 197 926 96 361 998 439 687 248
Cuts at: 4772 5113 5526 7436 7805 8967 9213 10494 11382
Size: 341 413 1910 369 1162 246 1281 888
Cuts at: 11382 12039 12425 12665 13678 14579 14841 820
Size: 657 386 240 1013 901 262 1553
Fragments arranged by size:
1910 1553 1281 1162 1013 998 926 901
888 687 657 439 413 386 369 361
341 262 248 246 240 197 96
SnaBI TAC'GTA
Cuts at: 493 13365 493
Size: 12872 2702
SpeI A'CTAG_T
Cuts at: 152 11413 152
Size: 11261 4313
SphI G_CATG'C
Cuts at: 1132 3094 3424 12932 1132
Size: 1962 330 9508 3774
Fragments arranged by size:
9508 3774 1962 330
SspI AAT'ATT
Cuts at: 5 52 13498 5
Size: 47 13446 2081
Fragments arranged by size:
13446 2081 47
StuI AGG'CCT
Cuts at: 1517 7738 8432 8731 9452 1517
Size: 6221 694 299 721 7639
Fragments arranged by size:
7639 6221 721 694 299
StyI C'CwwG_G
Cuts at: 513 1065 2489 3153 3874 5717 7611 7785 7832
Size: 552 1424 664 721 1843 1894 174 47
Cuts at: 7832 7867 7962 7971 8775 9795 10605 10791 10991
Size: 35 95 9 804 1020 810 186 200
Cuts at: 10991 11752 13013 513
Size: 761 1261 3074
Fragments arranged by size:
3074 1894 1843 1424 1261 1020 810 804
761 721 664 552 200 186 174 95
47 35 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2598 2848 6073 7604 8384 9280 10254
Size: 1255 1018 250 3225 1531 780 896 974
Cuts at: 10254 10367 10541 10667 10694 11112 11150 11993 12146
Size: 113 174 126 27 418 38 843 153
Cuts at: 12146 12477 12671 13601 13618 13771 13956 14401 325
Size: 331 194 930 17 153 185 445 1498
Fragments arranged by size:
3225 1531 1498 1255 1018 974 930 896
843 780 445 418 331 250 194 185
174 153 153 126 113 38 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 9004 9074 11012
Size: 274 80 33 51 1443 7027 70 1938
Cuts at: 11012 13820 15532 96
Size: 2808 1712 138
Fragments arranged by size:
7027 2808 1938 1712 1443 274 138 80
70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3258 3290 3598 3630 10229 10261 10650 10682 11839
Size: 32 308 32 6599 32 389 32 1157
Cuts at: 11839 11871 12338 12370 15534 15566 3258
Size: 32 467 32 3164 32 3266
Fragments arranged by size:
6599 3266 3164 1157 467 389 308 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4601 10769 11143 12658 1316
Size: 3285 6168 374 1515 4232
Fragments arranged by size:
6168 4232 3285 1515 374
XbaI T'CTAG_A
Cuts at: 2630 2669 2702 4952 6517 6820 12910 2630
Size: 39 33 2250 1565 303 6090 5294
Fragments arranged by size:
6090 5294 2250 1565 303 39 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 7678 7818 7927 8137 8270 11050 12461 2179
Size: 5499 140 109 210 133 2780 1411 5292
Fragments arranged by size:
5499 5292 2780 1411 210 140 133 109
XhoI C'TCGA_G
Cuts at: 1943 2400 3837 4524 11382 1943
Size: 457 1437 687 6858 6135
Fragments arranged by size:
6858 6135 1437 687 457
XmaI C'CCGG_G
Cuts at: 1870 2556 7094 10973 12899 1870
Size: 686 4538 3879 1926 4545
Fragments arranged by size:
4545 4538 3879 1926 686
XmnI GAAnn'nnTTC
Cuts at: 11229 13703 11229
Size: 2474 13100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 13382 276
Size: 53 83 186 936 2545 9303 2468
Fragments arranged by size:
9303 2545 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BplI BsiWI BstZ17I FseI FspAI
NsiI PacI PmlI RsrII SfiI SrfI SwaI