Restriction map for
pFN21AA0929
(Linear) (Six-base) MAPSORT of: KIAA0929.seq Check: 5648 from: 1 to: 10869
KIAA0929.orf
With 182 enzymes: SgfI *
November 17, 2015 09:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3142 6619 10869
Size: 3142 3477 4250
Fragments arranged by size:
4250 3477 3142
AatII G_ACGT'C
Cuts at: 0 2076 10869
Size: 2076 8793
AccI GT'mk_AC
Cuts at: 0 4976 7411 10869
Size: 4976 2435 3458
Fragments arranged by size:
4976 3458 2435
AclI AA'CG_TT
Cuts at: 0 10715 10869
Size: 10715 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 959 980 2366 2611 3362 3896 5303
Size: 269 690 21 1386 245 751 534 1407
Cuts at: 5303 5674 8330 8779 9935 10402 10520 10869
Size: 371 2656 449 1156 467 118 349
Fragments arranged by size:
2656 1407 1386 1156 751 690 534 467
449 371 349 269 245 118 21
AfeI AGC'GCT
Cuts at: 0 1255 2227 9224 10869
Size: 1255 972 6997 1645
Fragments arranged by size:
6997 1645 1255 972
AflIII A'CryG_T
Cuts at: 0 2939 5902 6332 9480 10869
Size: 2939 2963 430 3148 1389
Fragments arranged by size:
3148 2963 2939 1389 430
AgeI A'CCGG_T
Cuts at: 0 7191 9794 10869
Size: 7191 2603 1075
Fragments arranged by size:
7191 2603 1075
AleI CACnn'nnGTG
Cuts at: 0 2560 5923 8005 10869
Size: 2560 3363 2082 2864
Fragments arranged by size:
3363 2864 2560 2082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9082 9996 10045 10869
Size: 9082 914 49 824
Fragments arranged by size:
9082 914 824 49
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 203 235 2253 2285 3639 3671 10869
Size: 203 32 2018 32 1354 32 7198
Fragments arranged by size:
7198 2018 1354 203 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 648 823 4741 5134 5224 5281 5311 6642
Size: 648 175 3918 393 90 57 30 1331
Cuts at: 6642 7558 9391 9717 10410 10438 10869
Size: 916 1833 326 693 28 431
Fragments arranged by size:
3918 1833 1331 916 693 648 431 393
326 175 90 57 30 28
ApaI G_GGCC'C
Cuts at: 0 5932 7943 8891 9276 9907 10511 10869
Size: 5932 2011 948 385 631 604 358
Fragments arranged by size:
5932 2011 948 631 604 385 358
ApaLI G'TGCA_C
Cuts at: 0 6632 9763 10869
Size: 6632 3131 1106
Fragments arranged by size:
6632 3131 1106
ApoI r'AATT_y
Cuts at: 0 13 949 958 1138 1168 3005 3799 3870
Size: 13 936 9 180 30 1837 794 71
Cuts at: 3870 4165 4293 7104 7773 8413 10306 10869
Size: 295 128 2811 669 640 1893 563
Fragments arranged by size:
2811 1893 1837 936 794 669 640 563
295 180 128 71 30 13 9
AvaI C'yCGr_G
Cuts at: 0 395 551 1832 1907 2519 5089 5450 8611
Size: 395 156 1281 75 612 2570 361 3161
Cuts at: 8611 8883 8968 9236 9343 9377 10869
Size: 272 85 268 107 34 1492
Fragments arranged by size:
3161 2570 1492 1281 612 395 361 272
268 156 107 85 75 34
AvrII C'CTAG_G
Cuts at: 0 484 3712 10869
Size: 484 3228 7157
Fragments arranged by size:
7157 3228 484
BamHI G'GATC_C
Cuts at: 0 3696 7340 10869
Size: 3696 3644 3529
Fragments arranged by size:
3696 3644 3529
BanI G'GyrC_C
Cuts at: 0 4008 4869 5576 6869 9531 10869
Size: 4008 861 707 1293 2662 1338
Fragments arranged by size:
4008 2662 1338 1293 861 707
BanII G_rGCy'C
Cuts at: 0 422 492 869 4646 5645 5932 5956 7357
Size: 422 70 377 3777 999 287 24 1401
Cuts at: 7357 7407 7900 7943 8281 8618 8891 9243 9276
Size: 50 493 43 338 337 273 352 33
Cuts at: 9276 9907 9926 10511 10869
Size: 631 19 585 358
Fragments arranged by size:
3777 1401 999 631 585 493 422 377
358 352 338 337 287 273 70 50
43 33 24 19
BbeI G_GCGC'C
Cuts at: 0 4873 5580 6873 10869
Size: 4873 707 1293 3996
Fragments arranged by size:
4873 3996 1293 707
BbsI GAAGACnn'nnnn_
Cuts at: 0 2372 3263 3274 4118 4849 4897 6335 8350
Size: 2372 891 11 844 731 48 1438 2015
Cuts at: 8350 10869
Size: 2519
Fragments arranged by size:
2519 2372 2015 1438 891 844 731 48
11
BbvCI CC'TCA_GC
Cuts at: 0 2965 5975 6422 6750 9554 9938 10869
Size: 2965 3010 447 328 2804 384 931
Fragments arranged by size:
3010 2965 2804 931 447 384 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1469 1503 3345 3379 10869
Size: 1469 34 1842 34 7490
Fragments arranged by size:
7490 1842 1469 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 9691 10869
Size: 9691 1178
BclI T'GATC_A
Cuts at: 0 3237 10869
Size: 3237 7632
BglI GCCn_nnn'nGGC
Cuts at: 0 6448 9547 10696 10869
Size: 6448 3099 1149 173
Fragments arranged by size:
6448 3099 1149 173
BglII A'GATC_T
Cuts at: 0 3990 6112 6612 10757 10869
Size: 3990 2122 500 4145 112
Fragments arranged by size:
4145 3990 2122 500 112
BlpI GC'TnA_GC
Cuts at: 0 5225 10869
Size: 5225 5644
Bme1580I G_kGCm'C
Cuts at: 0 5932 6636 7160 7943 8026 8891 9071 9276
Size: 5932 704 524 783 83 865 180 205
Cuts at: 9276 9767 9907 10143 10511 10869
Size: 491 140 236 368 358
Fragments arranged by size:
5932 865 783 704 524 491 368 358
236 205 180 140 83
BmgBI CAC'GTC
Cuts at: 0 2940 5905 6333 10869
Size: 2940 2965 428 4536
Fragments arranged by size:
4536 2965 2940 428
BmrI ACTGGGnnnn_n'
Cuts at: 0 506 615 831 3148 3656 6276 6360 9058
Size: 506 109 216 2317 508 2620 84 2698
Cuts at: 9058 9066 9188 9692 10869
Size: 8 122 504 1177
Fragments arranged by size:
2698 2620 2317 1177 508 506 504 216
122 109 84 8
BmtI G_CTAG'C
Cuts at: 0 656 1340 10869
Size: 656 684 9529
Fragments arranged by size:
9529 684 656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 776 1775 2108 2830 5288 6108 6409
Size: 696 80 999 333 722 2458 820 301
Cuts at: 6409 6617 6713 7406 7452 7625 7694 7988 9066
Size: 208 96 693 46 173 69 294 1078
Cuts at: 9066 9694 9753 10561 10869
Size: 628 59 808 308
Fragments arranged by size:
2458 1078 999 820 808 722 696 693
628 333 308 301 294 208 173 96
80 69 59 46
Bpu10I CC'TnA_GC
Cuts at: 0 2774 2965 5975 6422 6750 7081 8280 9145
Size: 2774 191 3010 447 328 331 1199 865
Cuts at: 9145 9554 9899 9938 10115 10869
Size: 409 345 39 177 754
Fragments arranged by size:
3010 2774 1199 865 754 447 409 345
331 328 191 177 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 1378 2752 3093 3506 5416 5785 6947
Size: 19 1359 1374 341 413 1910 369 1162
Cuts at: 6947 7193 8474 10019 10405 10645 10869
Size: 246 1281 1545 386 240 224
Fragments arranged by size:
1910 1545 1374 1359 1281 1162 413 386
369 341 246 240 224 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 1582 2015 2637 3740 8776 10350 10869
Size: 1582 433 622 1103 5036 1574 519
Fragments arranged by size:
5036 1582 1574 1103 622 519 433
BsaAI yAC'GTr
Cuts at: 0 4123 10749 10869
Size: 4123 6626 120
Fragments arranged by size:
6626 4123 120
BsaBI GATnn'nnATC
Cuts at: 0 3695 10869
Size: 3695 7174
BsaHI Gr'CG_yC
Cuts at: 0 2073 4870 5210 5577 6870 10869
Size: 2073 2797 340 367 1293 3999
Fragments arranged by size:
3999 2797 2073 1293 367 340
BsaWI w'CCGG_w
Cuts at: 0 2159 2271 7191 9794 10869
Size: 2159 112 4920 2603 1075
Fragments arranged by size:
4920 2603 2159 1075 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 429 459 1253 1283 7404 7434 7450 7480
Size: 429 30 794 30 6121 30 16 30
Cuts at: 7480 7623 7653 8645 8675 10714 10744 10869
Size: 143 30 992 30 2039 30 125
Fragments arranged by size:
6121 2039 992 794 429 143 125 30
30 30 30 30 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 629 2046 2148 3533 5438 5779 5782 5992
Size: 629 1417 102 1385 1905 341 3 210
Cuts at: 5992 5995 7046 7049 7216 7408 7796 8942 9224
Size: 3 1051 3 167 192 388 1146 282
Cuts at: 9224 9361 9899 9926 10869
Size: 137 538 27 943
Fragments arranged by size:
1905 1417 1385 1146 1051 943 629 538
388 341 282 210 192 167 137 102
27 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1398 3115 5144 5274 6576 7181 7261 7936
Size: 1398 1717 2029 130 1302 605 80 675
Cuts at: 7936 8848 8890 8898 9001 9254 9306 9332 9830
Size: 912 42 8 103 253 52 26 498
Cuts at: 9830 9965 9992 10089 10270 10869
Size: 135 27 97 181 599
Fragments arranged by size:
2029 1717 1398 1302 912 675 605 599
498 253 181 135 130 103 97 80
52 42 27 26 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1000 3573 6479 8087 8108 9305 9415
Size: 809 191 2573 2906 1608 21 1197 110
Cuts at: 9415 9418 9775 9956 9967 10462 10869
Size: 3 357 181 11 495 407
Fragments arranged by size:
2906 2573 1608 1197 809 495 407 357
191 181 110 21 11 3
BsiEI CG_ry'CG
Cuts at: 0 5824 8262 10869
Size: 5824 2438 2607
Fragments arranged by size:
5824 2607 2438
BsiHKAI G_wGCw'C
Cuts at: 0 4646 5956 6636 7930 8324 9767 10869
Size: 4646 1310 680 1294 394 1443 1102
Fragments arranged by size:
4646 1443 1310 1294 1102 680 394
BsmI GAATG_Cn'
Cuts at: 0 7227 7878 8037 8079 10869
Size: 7227 651 159 42 2790
Fragments arranged by size:
7227 2790 651 159 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2099 2286 4917 7860 10461 10869
Size: 2099 187 2631 2943 2601 408
Fragments arranged by size:
2943 2631 2601 2099 408 187
Bsp1286I G_dGCh'C
Cuts at: 0 422 492 869 4646 5645 5932 5956 6636
Size: 422 70 377 3777 999 287 24 680
Cuts at: 6636 7160 7357 7407 7900 7930 7943 8026 8281
Size: 524 197 50 493 30 13 83 255
Cuts at: 8281 8324 8618 8891 9071 9243 9276 9767 9907
Size: 43 294 273 180 172 33 491 140
Cuts at: 9907 9926 10143 10511 10869
Size: 19 217 368 358
Fragments arranged by size:
3777 999 680 524 493 491 422 377
368 358 294 287 273 255 217 197
180 172 140 83 70 50 43 33
30 24 19 13
BspEI T'CCGG_A
Cuts at: 0 2271 10869
Size: 2271 8598
BspHI T'CATG_A
Cuts at: 0 465 10847 10869
Size: 465 10382 22
Fragments arranged by size:
10382 465 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3142 3216 4950 4988 5796 6619 10107 10691
Size: 3142 74 1734 38 808 823 3488 584
Cuts at: 10691 10869
Size: 178
Fragments arranged by size:
3488 3142 1734 823 808 584 178 74
38
BsrBI CCG'CTC
Cuts at: 0 402 579 5627 10869
Size: 402 177 5048 5242
Fragments arranged by size:
5242 5048 402 177
BsrDI GCAATG_nn'
Cuts at: 0 7547 7715 8408 10856 10869
Size: 7547 168 693 2448 13
Fragments arranged by size:
7547 2448 693 168 13
BsrFI r'CCGG_y
Cuts at: 0 5747 6075 6754 7191 8799 9794 10869
Size: 5747 328 679 437 1608 995 1075
Fragments arranged by size:
5747 1608 1075 995 679 437 328
BssSI C'ACGA_G
Cuts at: 0 3502 4247 10869
Size: 3502 745 6622
Fragments arranged by size:
6622 3502 745
BstAPI GCAn_nnn'nTGC
Cuts at: 0 823 4930 8074 8176 8506 9762 10410 10869
Size: 823 4107 3144 102 330 1256 648 459
Fragments arranged by size:
4107 3144 1256 823 648 459 330 102
BstBI TT'CG_AA
Cuts at: 0 1453 1785 3972 4299 10869
Size: 1453 332 2187 327 6570
Fragments arranged by size:
6570 2187 1453 332 327
BstEII G'GTnAC_C
Cuts at: 0 605 5050 5611 7251 9741 10869
Size: 605 4445 561 1640 2490 1128
Fragments arranged by size:
4445 2490 1640 1128 605 561
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1258 5925 6381 8403 10869
Size: 1258 4667 456 2022 2466
Fragments arranged by size:
4667 2466 2022 1258 456
BstYI r'GATC_y
Cuts at: 0 390 706 2805 3696 3990 4553 5124 6112
Size: 390 316 2099 891 294 563 571 988
Cuts at: 6112 6612 7340 8384 10757 10869
Size: 500 728 1044 2373 112
Fragments arranged by size:
2373 2099 1044 988 891 728 571 563
500 390 316 294 112
Bsu36I CC'TnA_GG
Cuts at: 0 1805 5810 5909 7477 10869
Size: 1805 4005 99 1568 3392
Fragments arranged by size:
4005 3392 1805 1568 99
BtgI C'CryG_G
Cuts at: 0 5606 5957 6398 7399 8015 9451 9512 9747
Size: 5606 351 441 1001 616 1436 61 235
Cuts at: 9747 10361 10869
Size: 614 508
Fragments arranged by size:
5606 1436 1001 616 614 508 441 351
235 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6393 8450 10869
Size: 6393 2057 2419
Fragments arranged by size:
6393 2419 2057
BtsI GCAGTG_nn'
Cuts at: 0 505 756 765 1665 5213 5378 6140 6798
Size: 505 251 9 900 3548 165 762 658
Cuts at: 6798 7288 8075 8096 8177 10869
Size: 490 787 21 81 2692
Fragments arranged by size:
3548 2692 900 787 762 658 505 490
251 165 81 21 9
ClaI AT'CG_AT
Cuts at: 0 3365 10869
Size: 3365 7504
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 7530 7565 10869
Size: 7530 35 3304
Fragments arranged by size:
7530 3304 35
DraI TTT'AAA
Cuts at: 0 2855 3797 10869
Size: 2855 942 7072
Fragments arranged by size:
7072 2855 942
DraIII CAC_nnn'GTG
Cuts at: 0 6810 9283 10869
Size: 6810 2473 1586
Fragments arranged by size:
6810 2473 1586
DrdI GACnn_nn'nnGTC
Cuts at: 0 10207 10869
Size: 10207 662
EaeI y'GGCC_r
Cuts at: 0 3845 6527 8138 8393 8516 8801 9214 10008
Size: 3845 2682 1611 255 123 285 413 794
Cuts at: 10008 10620 10869
Size: 612 249
Fragments arranged by size:
3845 2682 1611 794 612 413 285 255
249 123
EarI CTCTTCn'nnn_
Cuts at: 0 995 1719 3782 4245 4424 4880 5398 5756
Size: 995 724 2063 463 179 456 518 358
Cuts at: 5756 7433 10220 10869
Size: 1677 2787 649
Fragments arranged by size:
2787 2063 1677 995 724 649 518 463
456 358 179
EcoICRI GAG'CTC
Cuts at: 0 4644 5954 10869
Size: 4644 1310 4915
Fragments arranged by size:
4915 4644 1310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 696 776 959 980 1775 2108 2366
Size: 269 427 80 183 21 795 333 258
Cuts at: 2366 2611 2830 3362 3896 5288 5303 5674 6108
Size: 245 219 532 534 1392 15 371 434
Cuts at: 6108 6409 6617 6713 7406 7452 7625 7694 7988
Size: 301 208 96 693 46 173 69 294
Cuts at: 7988 8330 8779 9066 9694 9753 9935 10402 10520
Size: 342 449 287 628 59 182 467 118
Cuts at: 10520 10561 10869
Size: 41 308
Fragments arranged by size:
1392 795 693 628 534 532 467 449
434 427 371 342 333 308 301 294
287 269 258 245 219 208 183 182
173 118 96 80 69 59 46 41
21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 7582 9157 9914 10869
Size: 7582 1575 757 955
Fragments arranged by size:
7582 1575 955 757
EcoO109I rG'GnC_Cy
Cuts at: 0 677 5093 5831 5929 6465 7587 7885 7960
Size: 677 4416 738 98 536 1122 298 75
Cuts at: 7960 8485 8790 8887 9272 9273 9549 9903 9904
Size: 525 305 97 385 1 276 354 1
Cuts at: 9904 10058 10407 10507 10508 10869
Size: 154 349 100 1 361
Fragments arranged by size:
4416 1122 738 677 536 525 385 361
354 349 305 298 276 154 100 98
97 75 1 1 1
EcoRI G'AATT_C
Cuts at: 0 3870 7104 10306 10869
Size: 3870 3234 3202 563
Fragments arranged by size:
3870 3234 3202 563
EcoRV GAT'ATC
Cuts at: 0 531 3585 10869
Size: 531 3054 7284
Fragments arranged by size:
7284 3054 531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 955 1090 2599 2764 3025 4234 5528 6865
Size: 955 135 1509 165 261 1209 1294 1337
Cuts at: 6865 7541 7806 10686 10869
Size: 676 265 2880 183
Fragments arranged by size:
2880 1509 1337 1294 1209 955 676 265
261 183 165 135
HaeII r_GCGC'y
Cuts at: 0 1257 2229 2838 4873 5580 6643 6873 8372
Size: 1257 972 609 2035 707 1063 230 1499
Cuts at: 8372 9025 9226 10869
Size: 653 201 1643
Fragments arranged by size:
2035 1643 1499 1257 1063 972 707 653
609 230 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 429 461 996 1028 2929 2961
Size: 241 32 156 32 535 32 1901 32
Cuts at: 2961 3479 3511 4264 4296 6199 6231 6259 6291
Size: 518 32 753 32 1903 32 28 32
Cuts at: 6291 6667 6699 7093 7125 7450 7482 8244 8263
Size: 376 32 394 32 325 32 762 19
Cuts at: 8263 8276 8295 8581 8613 9882 9914 10869
Size: 13 19 286 32 1269 32 955
Fragments arranged by size:
1903 1901 1269 955 762 753 535 518
394 376 325 286 241 156 32 32
32 32 32 32 32 32 32 32
32 32 32 28 19 19 13
HincII GTy'rAC
Cuts at: 0 4326 7458 7605 8186 8871 10869
Size: 4326 3132 147 581 685 1998
Fragments arranged by size:
4326 3132 1998 685 581 147
HindIII A'AGCT_T
Cuts at: 0 2581 4211 5467 8752 10869
Size: 2581 1630 1256 3285 2117
Fragments arranged by size:
3285 2581 2117 1630 1256
KasI G'GCGC_C
Cuts at: 0 4869 5576 6869 10869
Size: 4869 707 1293 4000
Fragments arranged by size:
4869 4000 1293 707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3693 5107 6977 7165 10853 10869
Size: 3693 1414 1870 188 3688 16
Fragments arranged by size:
3693 3688 1870 1414 188 16
MscI TGG'CCA
Cuts at: 0 3847 8518 10869
Size: 3847 4671 2351
Fragments arranged by size:
4671 3847 2351
MslI CAynn'nnrTG
Cuts at: 0 2560 3689 4651 5923 8005 8248 9043 9064
Size: 2560 1129 962 1272 2082 243 795 21
Cuts at: 9064 9479 10869
Size: 415 1390
Fragments arranged by size:
2560 2082 1390 1272 1129 962 795 415
243 21
MspA1I CmG'CkG
Cuts at: 0 692 1579 1653 3222 4965 5368 5704 5848
Size: 692 887 74 1569 1743 403 336 144
Cuts at: 5848 8059 8104 8361 8715 8929 9246 9310 9514
Size: 2211 45 257 354 214 317 64 204
Cuts at: 9514 9773 9895 10093 10435 10554 10869
Size: 259 122 198 342 119 315
Fragments arranged by size:
2211 1743 1569 887 692 403 354 342
336 317 315 259 257 214 204 198
144 122 119 74 64 45
NaeI GCC'GGC
Cuts at: 0 5749 6756 8801 10869
Size: 5749 1007 2045 2068
Fragments arranged by size:
5749 2068 2045 1007
NarI GG'CG_CC
Cuts at: 0 4870 5577 6870 10869
Size: 4870 707 1293 3999
Fragments arranged by size:
4870 3999 1293 707
NcoI C'CATG_G
Cuts at: 0 5606 5957 9747 10869
Size: 5606 351 3790 1122
Fragments arranged by size:
5606 3790 1122 351
NgoMIV G'CCGG_C
Cuts at: 0 5747 6754 8799 10869
Size: 5747 1007 2045 2070
Fragments arranged by size:
5747 2070 2045 1007
NheI G'CTAG_C
Cuts at: 0 652 1336 10869
Size: 652 684 9533
Fragments arranged by size:
9533 684 652
NruI TCG'CGA
Cuts at: 0 494 10869
Size: 494 10375
NspI r_CATG'y
Cuts at: 0 1089 1419 9484 10869
Size: 1089 330 8065 1385
Fragments arranged by size:
8065 1385 1089 330
PasI CC'CwG_GG
Cuts at: 0 6182 8236 8649 8676 9159 9506 10869
Size: 6182 2054 413 27 483 347 1363
Fragments arranged by size:
6182 2054 1363 483 413 347 27
PciI A'CATG_T
Cuts at: 0 9480 10869
Size: 9480 1389
PflMI CCAn_nnn'nTGG
Cuts at: 0 10400 10869
Size: 10400 469
PfoI T'CCnGG_A
Cuts at: 0 2085 6594 6692 9880 10869
Size: 2085 4509 98 3188 989
Fragments arranged by size:
4509 3188 2085 989 98
PpuMI rG'GwC_Cy
Cuts at: 0 677 5831 8790 10058 10869
Size: 677 5154 2959 1268 811
Fragments arranged by size:
5154 2959 1268 811 677
PshAI GACnn'nnGTC
Cuts at: 0 8261 8788 9490 10869
Size: 8261 527 702 1379
Fragments arranged by size:
8261 1379 702 527
PspOMI G'GGCC_C
Cuts at: 0 5928 7939 8887 9272 9903 10507 10869
Size: 5928 2011 948 385 631 604 362
Fragments arranged by size:
5928 2011 948 631 604 385 362
PspXI vC'TCGA_Gb
Cuts at: 0 1832 2519 9377 10869
Size: 1832 687 6858 1492
Fragments arranged by size:
6858 1832 1492 687
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1283 1315 4153 4185 10869
Size: 1283 32 2838 32 6684
Fragments arranged by size:
6684 2838 1283 32 32
PstI C_TGCA'G
Cuts at: 0 816 2787 3227 5220 5373 5532 5703 5793
Size: 816 1971 440 1993 153 159 171 90
Cuts at: 5793 5847 6195 6647 7308 8502 9057 9251 9932
Size: 54 348 452 661 1194 555 194 681
Cuts at: 9932 10688 10757 10869
Size: 756 69 112
Fragments arranged by size:
1993 1971 1194 816 756 681 661 555
452 440 348 194 171 159 153 112
90 69 54
PvuI CG_AT'CG
Cuts at: 0 8262 10869
Size: 8262 2607
PvuII CAG'CTG
Cuts at: 0 1579 1653 3222 4965 5368 5848 8715 8929
Size: 1579 74 1569 1743 403 480 2867 214
Cuts at: 8929 9310 9895 10093 10435 10554 10869
Size: 381 585 198 342 119 315
Fragments arranged by size:
2867 1743 1579 1569 585 480 403 381
342 315 214 198 119 74
SacI G_AGCT'C
Cuts at: 0 4646 5956 10869
Size: 4646 1310 4913
Fragments arranged by size:
4913 4646 1310
SacII CC_GC'GG
Cuts at: 0 9515 10869
Size: 9515 1354
SanDI GG'GwC_CC
Cuts at: 0 10058 10869
Size: 10058 811
SapI GCTCTTCn'nnn_
Cuts at: 0 4424 7433 10869
Size: 4424 3009 3436
Fragments arranged by size:
4424 3436 3009
SbfI CC_TGCA'GG
Cuts at: 0 8502 10688 10869
Size: 8502 2186 181
Fragments arranged by size:
8502 2186 181
ScaI AGT'ACT
Cuts at: 0 9009 10869
Size: 9009 1860
SexAI A'CCwGG_T
Cuts at: 0 3999 10869
Size: 3999 6870
SfcI C'TryA_G
Cuts at: 0 384 534 812 914 2172 2783 3223 5216
Size: 384 150 278 102 1258 611 440 1993
Cuts at: 5216 5369 5528 5699 5789 5843 6191 6643 6710
Size: 153 159 171 90 54 348 452 67
Cuts at: 6710 6929 7304 8498 8861 9053 9204 9247 9928
Size: 219 375 1194 363 192 151 43 681
Cuts at: 9928 10684 10753 10869
Size: 756 69 116
Fragments arranged by size:
1993 1258 1194 756 681 611 452 440
384 375 363 348 278 219 192 171
159 153 151 150 116 102 90 69
67 54 43
SfoI GGC'GCC
Cuts at: 0 4871 5578 6871 10869
Size: 4871 707 1293 3998
Fragments arranged by size:
4871 3998 1293 707
SgrAI Cr'CCGG_yG
Cuts at: 0 5747 7191 10869
Size: 5747 1444 3678
Fragments arranged by size:
5747 3678 1444
SmaI CCC'GGG
Cuts at: 0 553 5091 8970 10869
Size: 553 4538 3879 1899
Fragments arranged by size:
4538 3879 1899 553
SmlI C'TyrA_G
Cuts at: 0 34 395 1393 1832 2519 2767 3108 3521
Size: 34 361 998 439 687 248 341 413
Cuts at: 3521 5431 5800 6962 7208 8489 9377 10034 10420
Size: 1910 369 1162 246 1281 888 657 386
Cuts at: 10420 10660 10869
Size: 240 209
Fragments arranged by size:
1910 1281 1162 998 888 687 657 439
413 386 369 361 341 248 246 240
209 34
SpeI A'CTAG_T
Cuts at: 0 9408 10869
Size: 9408 1461
SphI G_CATG'C
Cuts at: 0 1089 1419 10869
Size: 1089 330 9450
Fragments arranged by size:
9450 1089 330
StuI AGG'CCT
Cuts at: 0 5733 6427 6726 7447 10869
Size: 5733 694 299 721 3422
Fragments arranged by size:
5733 3422 721 694 299
StyI C'CwwG_G
Cuts at: 0 484 1148 1869 3712 5606 5780 5827 5862
Size: 484 664 721 1843 1894 174 47 35
Cuts at: 5862 5957 5966 6770 7790 8600 8786 8986 9747
Size: 95 9 804 1020 810 186 200 761
Cuts at: 9747 10869
Size: 1122
Fragments arranged by size:
1894 1843 1122 1020 810 804 761 721
664 484 200 186 174 95 47 35
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 593 843 4068 5599 6379 7275 8249 8362
Size: 593 250 3225 1531 780 896 974 113
Cuts at: 8362 8536 8662 8689 9107 9145 9988 10141 10472
Size: 174 126 27 418 38 843 153 331
Cuts at: 10472 10666 10869
Size: 194 203
Fragments arranged by size:
3225 1531 974 896 843 780 593 418
331 250 203 194 174 153 126 113
38 27
TatI w'GTAC_w
Cuts at: 0 6999 7069 9007 10869
Size: 6999 70 1938 1862
Fragments arranged by size:
6999 1938 1862 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1253 1285 1593 1625 8224 8256 8645 8677
Size: 1253 32 308 32 6599 32 389 32
Cuts at: 8677 9834 9866 10333 10365 10869
Size: 1157 32 467 32 504
Fragments arranged by size:
6599 1253 1157 504 467 389 308 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2596 8764 9138 10653 10869
Size: 2596 6168 374 1515 216
Fragments arranged by size:
6168 2596 1515 374 216
XbaI T'CTAG_A
Cuts at: 0 625 664 697 2947 4512 4815 10869
Size: 625 39 33 2250 1565 303 6054
Fragments arranged by size:
6054 2250 1565 625 303 39 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 5673 5813 5922 6132 6265 9045 10456
Size: 174 5499 140 109 210 133 2780 1411
Cuts at: 10456 10869
Size: 413
Fragments arranged by size:
5499 2780 1411 413 210 174 140 133
109
XhoI C'TCGA_G
Cuts at: 0 395 1832 2519 9377 10869
Size: 395 1437 687 6858 1492
Fragments arranged by size:
6858 1492 1437 687 395
XmaI C'CCGG_G
Cuts at: 0 551 5089 8968 10869
Size: 551 4538 3879 1901
Fragments arranged by size:
4538 3879 1901 551
XmnI GAAnn'nnTTC
Cuts at: 0 9224 10869
Size: 9224 1645
ZraI GAC'GTC
Cuts at: 0 2074 10869
Size: 2074 8795
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI
PasI PciI PflMI PfoI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AhdI AscI AseI AsiSI BaeI BfrBI
BplI BsiWI BsrGI BssHII BstZ17I EagI EciI FseI
FspI FspAI HpaI KpnI MfeI MluI NdeI NotI
NsiI PacI PmeI PmlI PpiI PsiI RsrII SalI
SfiI SgfI SnaBI SrfI SspI SwaI