(Circular) (Six-base) MAPSORT of: pFN21AA0926.seq Check: 4604 from: 1 to: 8992 pFN21AA0926 8992 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3031 3031 Size: 8992 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6802 278 Size: 53 83 186 936 5266 2468 Fragments arranged by size: 5266 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3658 6335 1937 Size: 1721 2677 4594 Fragments arranged by size: 4594 2677 1721 Acc65I G'GTAC_C Cuts at: 6313 6313 Size: 8992 AclI AA'CG_TT Cuts at: 1563 4190 7119 7492 1563 Size: 2627 2929 373 3063 Fragments arranged by size: 3063 2929 2627 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2087 3088 4289 4454 4796 4939 6236 Size: 440 15 1001 1201 165 342 143 1297 Cuts at: 6236 7055 8439 8707 1632 Size: 819 1384 268 1917 Fragments arranged by size: 1917 1384 1297 1201 1001 819 440 342 268 165 143 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8795 AflIII A'CryG_T Cuts at: 1203 4016 7891 8868 1203 Size: 2813 3875 977 1327 Fragments arranged by size: 3875 2813 1327 977 AgeI A'CCGG_T Cuts at: 5161 7823 5161 Size: 2662 6330 AhdI GACnn_n'nnGTC Cuts at: 2503 7721 2503 Size: 5218 3774 AleI CACnn'nnGTG Cuts at: 1166 2348 2546 5702 1166 Size: 1182 198 3156 4456 Fragments arranged by size: 4456 3156 1182 198 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3024 1184 Size: 1840 7152 AlwNI CAG_nnn'CTG Cuts at: 2083 3199 4132 4603 4669 4699 4744 4915 5410 Size: 1116 933 471 66 30 45 171 495 Cuts at: 5410 6007 6094 6735 8307 2083 Size: 597 87 641 1572 2768 Fragments arranged by size: 2768 1572 1116 933 641 597 495 471 171 87 66 45 30 ApaI G_GGCC'C Cuts at: 3550 4924 5249 3550 Size: 1374 325 7293 Fragments arranged by size: 7293 1374 325 ApaLI G'TGCA_C Cuts at: 2386 4264 5495 7049 8205 2386 Size: 1878 1231 1554 1156 3173 Fragments arranged by size: 3173 1878 1554 1231 1156 ApoI r'AATT_y Cuts at: 1493 2643 2834 4280 5387 6301 6527 6679 8788 Size: 1150 191 1446 1107 914 226 152 2109 Cuts at: 8788 1493 Size: 1697 Fragments arranged by size: 2109 1697 1446 1150 1107 914 226 191 152 AseI AT'TA_AT Cuts at: 160 1032 7546 160 Size: 872 6514 1606 Fragments arranged by size: 6514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8992 AvaI C'yCGr_G Cuts at: 1870 1943 3353 6045 6317 1870 Size: 73 1410 2692 272 4545 Fragments arranged by size: 4545 2692 1410 272 73 BamHI G'GATC_C Cuts at: 2364 2564 6322 6689 2364 Size: 200 3758 367 4667 Fragments arranged by size: 4667 3758 367 200 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6313 7768 618 Size: 325 217 627 4526 1455 1842 Fragments arranged by size: 4526 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2114 2399 3153 3282 3543 3550 4257 4346 Size: 723 285 754 129 261 7 707 89 Cuts at: 4346 4842 4924 5249 5799 6311 1391 Size: 496 82 325 550 512 4072 Fragments arranged by size: 4072 754 723 707 550 512 496 325 285 261 129 89 82 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 3088 5826 928 Size: 971 251 938 2738 4094 Fragments arranged by size: 4094 2738 971 938 251 BbvCI CC'TCA_GC Cuts at: 2240 2574 3732 4557 4941 2240 Size: 334 1158 825 384 6291 Fragments arranged by size: 6291 1158 825 384 334 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7183 7217 1903 Size: 34 5246 34 3678 Fragments arranged by size: 5246 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2965 4993 6884 8094 2965 Size: 2028 1891 1210 3863 Fragments arranged by size: 3863 2028 1891 1210 BclI T'GATC_A Cuts at: 1548 3369 1548 Size: 1821 7171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3179 3214 7603 Size: 107 122 71 631 605 1507 35 4389 Cuts at: 7603 136 Size: 1525 Fragments arranged by size: 4389 1525 1507 631 605 122 107 71 35 BglII A'GATC_T Cuts at: 5112 5932 8987 5112 Size: 820 3055 5117 Fragments arranged by size: 5117 3055 820 BlpI GC'TnA_GC Cuts at: 2133 3147 3687 4463 5472 6095 6409 2133 Size: 1014 540 776 1009 623 314 4716 Fragments arranged by size: 4716 1014 1009 776 623 540 314 Bme1580I G_kGCm'C Cuts at: 1790 2390 3550 3564 4268 4924 5249 5499 7053 Size: 600 1160 14 704 656 325 250 1554 Cuts at: 7053 8209 1790 Size: 1156 2573 Fragments arranged by size: 2573 1554 1160 1156 704 656 600 325 250 14 BmgBI CAC'GTC Cuts at: 1318 4725 5104 1318 Size: 3407 379 5206 Fragments arranged by size: 5206 3407 379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3118 4370 6723 Size: 416 53 83 399 24 1701 1252 2353 Cuts at: 6723 7681 442 Size: 958 1753 Fragments arranged by size: 2353 1753 1701 1252 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8992 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 2807 4524 5161 2182 Size: 625 1717 637 6013 Fragments arranged by size: 6013 1717 637 625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2467 3313 4172 4541 4665 4946 5499 Size: 850 378 846 859 369 124 281 553 Cuts at: 5499 5606 5813 5835 5946 7652 1239 Size: 107 207 22 111 1706 2579 Fragments arranged by size: 2579 1706 859 850 846 553 378 369 281 207 124 111 107 22 Bpu10I CC'TnA_GC Cuts at: 2240 2574 3732 4557 4619 4841 4941 5079 2240 Size: 334 1158 825 62 222 100 138 6153 Fragments arranged by size: 6153 1158 825 334 222 138 100 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4400 5018 5181 6187 7117 7982 8280 4400 Size: 618 163 1006 930 865 298 5112 Fragments arranged by size: 5112 1006 930 865 618 298 163 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2328 3705 4517 5351 6215 7655 882 Size: 617 829 1377 812 834 864 1440 2219 Fragments arranged by size: 2219 1440 1377 864 834 829 812 617 BsaAI yAC'GTr Cuts at: 493 1204 4294 6783 493 Size: 711 3090 2489 2702 Fragments arranged by size: 3090 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 8986 8986 Size: 8992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2440 4504 6799 7181 Size: 53 83 186 936 907 2064 2295 382 Cuts at: 7181 7816 275 Size: 635 1451 Fragments arranged by size: 2295 2064 1451 936 907 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5161 6183 7425 7823 8097 8244 8655 5161 Size: 1022 1242 398 274 147 411 5498 Fragments arranged by size: 5498 1242 1022 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3160 3166 3190 3196 1178 Size: 30 165 30 1757 6 24 6 6974 Fragments arranged by size: 6974 1757 165 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2459 2933 5585 1186 Size: 889 384 474 2652 4593 Fragments arranged by size: 4593 2652 889 474 384 BseYI C'CCAG_C Cuts at: 2327 2551 2559 2579 3322 3334 3702 4769 5633 Size: 224 8 20 743 12 368 1067 864 Cuts at: 5633 6140 8195 8691 2327 Size: 507 2055 496 2628 Fragments arranged by size: 2628 2055 1067 864 743 507 496 368 224 20 12 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3272 3568 5993 1163 Size: 573 1536 296 2425 4162 Fragments arranged by size: 4162 2425 1536 573 296 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6442 7203 8231 1540 Size: 275 186 4441 761 1028 2301 Fragments arranged by size: 4441 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2114 2390 3153 3282 3543 3843 4257 4268 4635 Size: 276 763 129 261 300 414 11 367 Cuts at: 4635 5499 5799 7053 7138 8209 2114 Size: 864 300 1254 85 1071 2897 Fragments arranged by size: 2897 1254 1071 864 763 414 367 300 300 276 261 129 85 11 BsmI GAATG_Cn' Cuts at: 6503 6596 6503 Size: 93 8899 BsmBI CGTCTCn'nnnn_ Cuts at: 4495 4495 Size: 8992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2390 2399 3153 3282 3543 3550 Size: 399 324 276 9 754 129 261 7 Cuts at: 3550 3564 3843 4257 4268 4346 4635 4842 4924 Size: 14 279 414 11 78 289 207 82 Cuts at: 4924 5249 5499 5799 6311 7053 7138 8209 1391 Size: 325 250 300 512 742 85 1071 2174 Fragments arranged by size: 2174 1071 754 742 512 414 399 325 324 300 289 279 276 261 250 207 129 85 82 78 14 11 9 7 BspEI T'CCGG_A Cuts at: 8655 8655 Size: 8992 BspHI T'CATG_A Cuts at: 6881 8611 6881 Size: 1730 7262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3031 4797 5214 6347 844 Size: 2187 1766 417 1133 3489 Fragments arranged by size: 3489 2187 1766 1133 417 BsrBI CCG'CTC Cuts at: 1394 1823 2132 3214 6879 1394 Size: 429 309 1082 3665 3507 Fragments arranged by size: 3665 3507 1082 429 309 BsrDI GCAATG_nn' Cuts at: 65 1248 3906 7487 7661 65 Size: 1183 2658 3581 174 1396 Fragments arranged by size: 3581 2658 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2377 3307 4080 5161 7636 7823 1700 Size: 110 567 930 773 1081 2475 187 2869 Fragments arranged by size: 2869 2475 1081 930 773 567 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8992 BssSI C'ACGA_G Cuts at: 6133 7052 8064 6133 Size: 919 1012 7061 Fragments arranged by size: 7061 1012 919 BstAPI GCAn_nnn'nTGC Cuts at: 794 4132 4272 6007 794 Size: 3338 140 1735 3779 Fragments arranged by size: 3779 3338 1735 140 BstBI TT'CG_AA Cuts at: 4218 7796 4218 Size: 3578 5414 BstEII G'GTnAC_C Cuts at: 1184 4420 5371 1184 Size: 3236 951 4805 Fragments arranged by size: 4805 3236 951 BstXI CCAn_nnnn'nTGG Cuts at: 3074 3074 Size: 8992 BstYI r'GATC_y Cuts at: 1972 2364 2564 2881 3195 5112 5932 6322 6689 Size: 392 200 317 314 1917 820 390 367 Cuts at: 6689 7074 7091 8543 8629 8641 8987 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1917 1452 820 392 390 385 367 346 317 314 200 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3776 5733 3776 Size: 1957 7035 BtgI C'CryG_G Cuts at: 513 1065 2004 2702 2765 4350 5250 513 Size: 552 939 698 63 1585 900 4255 Fragments arranged by size: 4255 1585 939 900 698 552 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8253 Fragments arranged by size: 8253 683 56 BtsI GCAGTG_nn' Cuts at: 2355 2553 3317 4075 4115 4162 4482 6508 7302 Size: 198 764 758 40 47 320 2026 794 Cuts at: 7302 7322 2355 Size: 20 4025 Fragments arranged by size: 4025 2026 794 764 758 320 198 47 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8957 DraI TTT'AAA Cuts at: 5566 6296 5566 Size: 730 8262 DraIII CAC_nnn'GTG Cuts at: 5512 6016 5512 Size: 504 8488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7999 809 Size: 507 6683 1802 Fragments arranged by size: 6683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3033 5848 6439 Size: 54 1425 50 275 18 1203 2815 591 Cuts at: 6439 7328 8957 8 Size: 889 1629 43 Fragments arranged by size: 2815 1629 1425 1203 889 591 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6439 1537 Size: 275 4627 4090 Fragments arranged by size: 4627 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2119 2487 3227 4409 4475 6922 1353 Size: 766 368 740 1182 66 2447 3423 Fragments arranged by size: 3423 2447 1182 766 740 368 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2028 3326 7579 7953 8099 387 Size: 1064 138 439 1298 4253 374 146 1280 Fragments arranged by size: 4253 1298 1280 1064 439 374 146 138 EcoICRI GAG'CTC Cuts at: 2112 3151 3280 3541 4255 5797 2112 Size: 1039 129 261 714 1542 5307 Fragments arranged by size: 5307 1542 1039 714 261 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2087 2089 2467 3088 3313 4172 Size: 393 440 15 2 378 621 225 859 Cuts at: 4172 4289 4454 4541 4665 4796 4939 4946 5499 Size: 117 165 87 124 131 143 7 553 Cuts at: 5499 5606 5813 5835 5946 6236 7055 7652 8439 Size: 107 207 22 111 290 819 597 787 Cuts at: 8439 8707 1239 Size: 268 1524 Fragments arranged by size: 1524 859 819 787 621 597 553 440 393 378 290 268 225 207 165 143 131 124 117 111 107 87 22 15 7 2 EcoNI CCTnn'n_nnAGG Cuts at: 2054 3671 4928 2054 Size: 1617 1257 6118 Fragments arranged by size: 6118 1617 1257 EcoO109I rG'GnC_Cy Cuts at: 2206 2613 2697 3436 3547 3698 4114 4372 4570 Size: 407 84 739 111 151 416 258 198 Cuts at: 4570 4741 4920 4921 4993 5203 5245 5246 5255 Size: 171 179 1 72 210 42 1 9 Cuts at: 5255 5450 2206 Size: 195 5748 Fragments arranged by size: 5748 739 416 407 258 210 198 195 179 171 151 111 84 72 42 9 1 1 EcoRI G'AATT_C Cuts at: 5387 6301 6679 5387 Size: 914 378 7700 Fragments arranged by size: 7700 914 378 FspI TGC'GCA Cuts at: 7498 7498 Size: 8992 HaeII r_GCGC'y Cuts at: 1678 8139 1678 Size: 6461 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2116 2148 2489 2521 2621 Size: 32 135 32 544 32 341 32 100 Cuts at: 2621 2653 3160 3192 3361 3373 3393 3405 3690 Size: 32 507 32 169 12 20 12 285 Cuts at: 3690 3704 3722 3736 4733 4765 5850 5882 6214 Size: 14 18 14 997 32 1085 32 332 Cuts at: 6214 6246 7633 7665 7707 7739 1373 Size: 32 1387 32 42 32 2626 Fragments arranged by size: 2626 1387 1085 997 544 507 341 332 285 169 135 100 42 32 32 32 32 32 32 32 32 32 32 32 20 18 14 14 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3027 5278 6336 6582 669 Size: 988 281 1089 2251 1058 246 3079 Fragments arranged by size: 3079 2251 1089 1058 988 281 246 HindIII A'AGCT_T Cuts at: 748 4301 748 Size: 3553 5439 HpaI GTT'AAC Cuts at: 6582 6582 Size: 8992 KpnI G_GTAC'C Cuts at: 6317 6317 Size: 8992 MfeI C'AATT_G Cuts at: 5121 6591 5121 Size: 1470 7522 MluI A'CGCG_T Cuts at: 8868 8868 Size: 8992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 8106 8290 1305 Size: 6801 184 2007 Fragments arranged by size: 6801 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3035 5850 10 Size: 54 1425 343 1203 2815 3152 Fragments arranged by size: 3152 2815 1425 1203 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2348 2546 5702 6950 7309 7468 Size: 648 993 189 198 3156 1248 359 159 Cuts at: 7468 518 Size: 2042 Fragments arranged by size: 3156 2042 1248 993 648 359 198 189 159 MspA1I CmG'CkG Cuts at: 1606 2197 2422 2445 2711 2845 3070 3108 4129 Size: 591 225 23 266 134 225 38 1021 Cuts at: 4129 4309 4556 4705 4713 4945 5331 5436 5827 Size: 180 247 149 8 232 386 105 391 Cuts at: 5827 6037 6408 7085 8233 8478 1606 Size: 210 371 677 1148 245 2120 Fragments arranged by size: 2120 1148 1021 677 591 391 386 371 266 247 245 232 225 225 210 180 149 134 105 38 23 8 NaeI GCC'GGC Cuts at: 2379 3309 4082 2379 Size: 930 773 7289 Fragments arranged by size: 7289 930 773 NcoI C'CATG_G Cuts at: 513 1065 2004 2702 2765 4350 513 Size: 552 939 698 63 1585 5155 Fragments arranged by size: 5155 1585 939 698 552 63 NdeI CA'TA_TG Cuts at: 387 3982 387 Size: 3595 5397 NgoMIV G'CCGG_C Cuts at: 2377 3307 4080 2377 Size: 930 773 7289 Fragments arranged by size: 7289 930 773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8992 NotI GC'GGCC_GC Cuts at: 6439 6439 Size: 8992 NspI r_CATG'y Cuts at: 1132 1229 3095 4234 6350 7895 1132 Size: 97 1866 1139 2116 1545 2229 Fragments arranged by size: 2229 2116 1866 1545 1139 97 PasI CC'CwG_GG Cuts at: 1348 2392 3564 1348 Size: 1044 1172 6776 Fragments arranged by size: 6776 1172 1044 PciI A'CATG_T Cuts at: 7891 7891 Size: 8992 PflMI CCAn_nnn'nTGG Cuts at: 1108 4744 5410 1108 Size: 3636 666 4690 Fragments arranged by size: 4690 3636 666 PfoI T'CCnGG_A Cuts at: 2105 4518 5097 5583 2105 Size: 2413 579 486 5514 Fragments arranged by size: 5514 2413 579 486 PmeI GTTT'AAAC Cuts at: 6296 6296 Size: 8992 PmlI CAC'GTG Cuts at: 4294 4294 Size: 8992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2779 2811 5781 5813 7061 7093 8574 8606 2779 Size: 32 2970 32 1248 32 1481 32 3165 Fragments arranged by size: 3165 2970 1481 1248 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2613 3436 3698 4114 4372 4570 4741 5203 Size: 407 823 262 416 258 198 171 462 Cuts at: 5203 2206 Size: 5995 Fragments arranged by size: 5995 823 462 416 407 262 258 198 171 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8992 PsiI TTA'TAA Cuts at: 6562 6562 Size: 8992 PspOMI G'GGCC_C Cuts at: 3546 4920 5245 3546 Size: 1374 325 7293 Fragments arranged by size: 7293 1374 325 PstI C_TGCA'G Cuts at: 830 3003 3113 3396 4128 4140 4477 4704 4794 Size: 2173 110 283 732 12 337 227 90 Cuts at: 4794 6344 830 Size: 1550 3478 Fragments arranged by size: 3478 2173 1550 732 337 283 227 110 90 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8992 PvuII CAG'CTG Cuts at: 2422 2845 3070 3108 4129 4309 4556 4705 4713 Size: 423 225 38 1021 180 247 149 8 Cuts at: 4713 4945 5436 5827 6037 2422 Size: 232 491 391 210 5377 Fragments arranged by size: 5377 1021 491 423 391 247 232 225 210 180 149 38 8 SacI G_AGCT'C Cuts at: 2114 3153 3282 3543 4257 5799 2114 Size: 1039 129 261 714 1542 5307 Fragments arranged by size: 5307 1542 1039 714 261 129 SalI G'TCGA_C Cuts at: 1936 6334 1936 Size: 4398 4594 SanDI GG'GwC_CC Cuts at: 2613 3698 4114 4372 2613 Size: 1085 416 258 7233 Fragments arranged by size: 7233 1085 416 258 SapI GCTCTTCn'nnn_ Cuts at: 2119 2487 3227 2119 Size: 368 740 7884 Fragments arranged by size: 7884 740 368 SbfI CC_TGCA'GG Cuts at: 6344 6344 Size: 8992 ScaI AGT'ACT Cuts at: 7240 8952 7240 Size: 1712 7280 SexAI A'CCwGG_T Cuts at: 2172 2172 Size: 8992 SfcI C'TryA_G Cuts at: 826 1045 2296 2747 2999 3109 3392 3475 3859 Size: 219 1251 451 252 110 283 83 384 Cuts at: 3859 4124 4136 4473 4691 4700 4790 5313 6340 Size: 265 12 337 218 9 90 523 1027 Cuts at: 6340 7475 8156 8347 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1251 1135 1027 681 523 451 384 337 283 265 252 219 218 191 110 90 83 12 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8992 SmaI CCC'GGG Cuts at: 1872 6319 1872 Size: 4447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4415 5033 5196 6166 7096 7997 Size: 197 926 2472 618 163 970 930 901 Cuts at: 7997 8259 820 Size: 262 1553 Fragments arranged by size: 2472 1553 970 930 926 901 618 262 197 163 SnaBI TAC'GTA Cuts at: 493 6783 493 Size: 6290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8992 SphI G_CATG'C Cuts at: 1132 6350 1132 Size: 5218 3774 SspI AAT'ATT Cuts at: 5 52 3470 6916 5 Size: 47 3418 3446 2081 Fragments arranged by size: 3446 3418 2081 47 StuI AGG'CCT Cuts at: 1517 2967 5915 1517 Size: 1450 2948 4594 Fragments arranged by size: 4594 2948 1450 StyI C'CwwG_G Cuts at: 513 1065 2004 2702 2765 3129 4350 5521 5646 Size: 552 939 698 63 364 1221 1171 125 Cuts at: 5646 5916 6431 513 Size: 270 515 3074 Fragments arranged by size: 3074 1221 1171 939 698 552 515 364 270 125 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2405 2539 2605 2778 3556 4622 5402 Size: 1255 825 134 66 173 778 1066 780 Cuts at: 5402 7019 7036 7189 7374 7819 325 Size: 1617 17 153 185 445 1498 Fragments arranged by size: 1617 1498 1255 1066 825 780 778 445 185 173 153 134 66 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7238 8950 96 Size: 274 80 33 51 1443 5261 1712 138 Fragments arranged by size: 5261 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3166 3198 4957 4989 5159 5191 6173 6205 8952 Size: 32 1759 32 170 32 982 32 2747 Cuts at: 8952 8984 3166 Size: 32 3174 Fragments arranged by size: 3174 2747 1759 982 170 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8992 XbaI T'CTAG_A Cuts at: 6328 6328 Size: 8992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2548 5257 5433 2548 Size: 2709 176 6107 Fragments arranged by size: 6107 2709 176 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8992 XmaI C'CCGG_G Cuts at: 1870 6317 1870 Size: 4447 4545 XmnI GAAnn'nnTTC Cuts at: 2072 6158 7121 2072 Size: 4086 963 3943 Fragments arranged by size: 4086 3943 963 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6800 276 Size: 53 83 186 936 5266 2468 Fragments arranged by size: 5266 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI