Restriction map for
pFN21AA0925
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflII AgeI AhdI AlwNI AocI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI BbeI BbvCI
BcgI BciVI BclI BfrI BglII
BlpI Bpu1102I BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrGI BsrI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 43 GTCTAC GT^[AC][GT]AC
AccIII 35 TCCGGA T^CCGGA
AcyI 8 GGCGTC G[GA]^CG[CT]C
AfaI 873 GTAC GT^AC
AflIII 311 ACACGT A^C[GA][CT]GT
Alw44I 103 GTGCAC G^TGCAC
Aor51HI 448 AGCGCT AGC^GCT
ApaLI 103 GTGCAC G^TGCAC
Asp700I 270 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 337 CCCGAG C^[CT]CG[GA]G
AviII 1403 TGCGCA TGC^GCA
AvrII 948 CCTAGG C^CTAGG
BamHI 83 GGATCC G^GATCC
BanI 419 GGCACC G^G[CT][GA]CC
BbrPI 576 CACGTG CAC^GTG
BbsI 1081 GAAGAC GAAGAC(2/6)
BfaI 949 CTAG C^TAG
BglI 239 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 948 CCTAGG C^CTAGG
BpuAI 1081 GAAGAC GAAGAC(2/6)
BsaAI 576 CACGTG [CT]AC^GT[GA]
BsaBI 84 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 8 GGCGTC G[GA]^CG[CT]C
BsaI 708 GGTCTC GGTCTC(1/5)
BseAI 35 TCCGGA T^CCGGA
BsgI 382 CTGCAC GTGCAG(16/14)
BsmAI 709 GTCTC GTCTC(1/5)
BsmI 1370 GCATTC GAATGC(1/-1)
BsoBI 337 CCCGAG C^[CT]CG[GA]G
BspEI 35 TCCGGA T^CCGGA
BssSI 297 CTCGTG CACGAG(-5/-1)
BstBI 1301 TTCGAA TT^CGAA
DrdI 460 GACTACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 448 AGCGCT AGC^GCT
EcoRV 259 GATATC GAT^ATC
FspI 1403 TGCGCA TGC^GCA
HgaI 9 GCGTC GACGC(5/10)
Hin1I 8 GGCGTC G[GA]^CG[CT]C
MaeI 949 CTAG C^TAG
MamI 84 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 35 TCCGGA T^CCGGA
MseI 1211 TTAA T^TAA
MspA1I 1384 CAGCTG C[AC]G^C[GT]G
NaeI 395 GCCGGC GCC^GGC
NgoMIV 395 GCCGGC G^CCGGC
NspV 1301 TTCGAA TT^CGAA
PleI 1017 GACTC GAGTC(4/5)
PmaCI 576 CACGTG CAC^GTG
PmlI 576 CACGTG CAC^GTG
PshAI 702 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 628 CTGCAG CTGCA^G
PvuII 1384 CAGCTG CAG^CTG
RsaI 873 GTAC GT^AC
SacI 1294 GAGCTC GAGCT^C
SfcI 628 CTGCAG C^T[GA][CT]AG
SfuI 1301 TTCGAA TT^CGAA
SmlI 1021 CTCAAG C^T[CT][GA]AG
StuI 945 AGGCCT AGG^CCT
Tru9I 1211 TTAA T^TAA
Tsp509I 783 AATT ^AATT
XcmI 962 CCAAGCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 270 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 56 TGGCCA TGG^CCA
143 TGGCCA
BsaWI 35 TCCGGA [AT]^CCGG[AT]
869 TCCGGT
BseRI 694 CTCCTC GAGGAG(10/8)
1345 GAGGAG
BsmFI 743 GGGAC GGGAC(10/14)
1273 GTCCC
BsrBI 612 CCGCTC CCGCTC(-3/-3)
924 CCGCTC
BsrDI 291 CATTGC GCAATG(2/0)
248 GCAATG
BsrFI 395 GCCGGC [GA]^CCGG[CT]
498 GCCGGT
Cfr10I 395 GCCGGC [GA]^CCGG[CT]
498 GCCGGT
DdeI 890 CTGAG C^T[ACGT]AG
904 CTGAG
DraIII 573 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
652 CACAGAGTG
EarI 775 GAAGAG CTCTTC(1/4)
1233 GAAGAG
EcoT14I 227 CCAAGG C^C[AT][AT]GG
948 CCTAGG
HaeII 369 GGCGCT [GA]GCGC^[CT]
448 AGCGCT
HincII 127 GTCAAC GT[CT]^[GA]AC
1246 GTCAAC
HindII 127 GTCAAC GT[CT]^[GA]AC
1246 GTCAAC
Ksp632I 775 GAAGAG CTCTTC(1/4)
1233 GAAGAG
MaeII 313 ACGT A^CGT
577 ACGT
MluNI 56 TGGCCA TGG^CCA
143 TGGCCA
MscI 56 TGGCCA TGG^CCA
143 TGGCCA
NspI 539 GCATGT [GA]CATG^[CT]
833 ACATGC
PpuMI 467 AGGTCCT [GA]G^G[AT]CC[CT]
752 AGGACCT
SfaNI 820 GATGC GCATC(5/9)
1145 GCATC
StyI 227 CCAAGG C^C[AT][AT]GG
948 CCTAGG
TfiI 1069 GATTC G^A[AT]TC
1329 GATTC
Tsp45I 151 GTCAC ^GT[GC]AC
1015 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
TseI TspRI TthHB8I XhoII