Restriction map for
pFN21AA0925
(Linear) (Six-base) MAPSORT of: KIAA0925.seq Check: 5705 from: 1 to: 1425
KIAA0925.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 44 1425
Size: 44 1381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1425
Size: 370 1055
AfeI AGC'GCT
Cuts at: 0 450 1425
Size: 450 975
AflIII A'CryG_T
Cuts at: 0 311 1425
Size: 311 1114
AleI CACnn'nnGTG
Cuts at: 0 119 316 526 1425
Size: 119 197 210 899
Fragments arranged by size:
899 210 197 119
ApaLI G'TGCA_C
Cuts at: 0 103 1425
Size: 103 1322
AvaI C'yCGr_G
Cuts at: 0 337 1425
Size: 337 1088
AvrII C'CTAG_G
Cuts at: 0 948 1425
Size: 948 477
BamHI G'GATC_C
Cuts at: 0 83 1425
Size: 83 1342
BanI G'GyrC_C
Cuts at: 0 419 1425
Size: 419 1006
BanII G_rGCy'C
Cuts at: 0 611 794 1040 1298 1425
Size: 611 183 246 258 127
Fragments arranged by size:
611 258 246 183 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 1088 1425
Size: 1088 337
BglI GCCn_nnn'nGGC
Cuts at: 0 245 1425
Size: 245 1180
Bme1580I G_kGCm'C
Cuts at: 0 107 392 1058 1425
Size: 107 285 666 367
Fragments arranged by size:
666 367 285 107
BmgBI CAC'GTC
Cuts at: 0 314 1425
Size: 314 1111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 630 774 942 1059 1425
Size: 630 144 168 117 366
Fragments arranged by size:
630 366 168 144 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 397 696 1366 1425
Size: 214 183 299 670 59
Fragments arranged by size:
670 299 214 183 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1425
Size: 1006 419
BsaI GGTCTCn'nnnn_
Cuts at: 0 714 1425
Size: 714 711
BsaAI yAC'GTr
Cuts at: 0 578 1425
Size: 578 847
BsaBI GATnn'nnATC
Cuts at: 0 88 1425
Size: 88 1337
BsaHI Gr'CG_yC
Cuts at: 0 9 1425
Size: 9 1416
BsaWI w'CCGG_w
Cuts at: 0 35 869 1425
Size: 35 834 556
Fragments arranged by size:
834 556 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 298 328 1425
Size: 10 30 258 30 1097
Fragments arranged by size:
1097 258 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 1360 1425
Size: 685 675 65
Fragments arranged by size:
685 675 65
BseYI C'CCAG_C
Cuts at: 0 930 1137 1425
Size: 930 207 288
Fragments arranged by size:
930 288 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1425
Size: 367 1058
BsiHKAI G_wGCw'C
Cuts at: 0 71 107 304 1298 1425
Size: 71 36 197 994 127
Fragments arranged by size:
994 197 127 71 36
BsmI GAATG_Cn'
Cuts at: 0 1370 1425
Size: 1370 55
Bsp1286I G_dGCh'C
Cuts at: 0 71 107 304 392 611 794 1040 1058
Size: 71 36 197 88 219 183 246 18
Cuts at: 1058 1298 1425
Size: 240 127
Fragments arranged by size:
246 240 219 197 183 127 88 71
36 18
BspEI T'CCGG_A
Cuts at: 0 35 1425
Size: 35 1390
BsrBI CCG'CTC
Cuts at: 0 614 926 1425
Size: 614 312 499
Fragments arranged by size:
614 499 312
BsrDI GCAATG_nn'
Cuts at: 0 255 290 1425
Size: 255 35 1135
Fragments arranged by size:
1135 255 35
BsrFI r'CCGG_y
Cuts at: 0 395 498 1425
Size: 395 103 927
Fragments arranged by size:
927 395 103
BssSI C'ACGA_G
Cuts at: 0 297 1425
Size: 297 1128
BstBI TT'CG_AA
Cuts at: 0 1302 1425
Size: 1302 123
BstYI r'GATC_y
Cuts at: 0 83 323 332 1155 1425
Size: 83 240 9 823 270
Fragments arranged by size:
823 270 240 83 9
BtsI GCAGTG_nn'
Cuts at: 0 69 1425
Size: 69 1356
DraIII CAC_nnn'GTG
Cuts at: 0 578 657 1425
Size: 578 79 768
Fragments arranged by size:
768 578 79
DrdI GACnn_nn'nnGTC
Cuts at: 0 466 1425
Size: 466 959
EaeI y'GGCC_r
Cuts at: 0 56 143 237 618 1425
Size: 56 87 94 381 807
Fragments arranged by size:
807 381 94 87 56
EarI CTCTTCn'nnn_
Cuts at: 0 770 1228 1425
Size: 770 458 197
Fragments arranged by size:
770 458 197
EcoICRI GAG'CTC
Cuts at: 0 1296 1425
Size: 1296 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 370 397 696 1366 1425
Size: 214 156 27 299 670 59
Fragments arranged by size:
670 299 214 156 59 27
EcoO109I rG'GnC_Cy
Cuts at: 0 468 753 1026 1425
Size: 468 285 273 399
Fragments arranged by size:
468 399 285 273
EcoRV GAT'ATC
Cuts at: 0 261 1425
Size: 261 1164
FspI TGC'GCA
Cuts at: 0 1405 1425
Size: 1405 20
HaeII r_GCGC'y
Cuts at: 0 373 452 1425
Size: 373 79 973
Fragments arranged by size:
973 373 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 778 810 1425
Size: 298 32 448 32 615
Fragments arranged by size:
615 448 298 32 32
HincII GTy'rAC
Cuts at: 0 129 1248 1425
Size: 129 1119 177
Fragments arranged by size:
1119 177 129
MscI TGG'CCA
Cuts at: 0 58 145 1425
Size: 58 87 1280
Fragments arranged by size:
1280 87 58
MslI CAynn'nnrTG
Cuts at: 0 119 316 524 526 832 1045 1051 1425
Size: 119 197 208 2 306 213 6 374
Fragments arranged by size:
374 306 213 208 197 119 6 2
MspA1I CmG'CkG
Cuts at: 0 1386 1425
Size: 1386 39
NaeI GCC'GGC
Cuts at: 0 397 1425
Size: 397 1028
NgoMIV G'CCGG_C
Cuts at: 0 395 1425
Size: 395 1030
NspI r_CATG'y
Cuts at: 0 543 837 1425
Size: 543 294 588
Fragments arranged by size:
588 543 294
PfoI T'CCnGG_A
Cuts at: 0 374 1425
Size: 374 1051
PmlI CAC'GTG
Cuts at: 0 578 1425
Size: 578 847
PpuMI rG'GwC_Cy
Cuts at: 0 468 753 1425
Size: 468 285 672
Fragments arranged by size:
672 468 285
PshAI GACnn'nnGTC
Cuts at: 0 706 1425
Size: 706 719
PstI C_TGCA'G
Cuts at: 0 632 1425
Size: 632 793
PvuII CAG'CTG
Cuts at: 0 1386 1425
Size: 1386 39
SacI G_AGCT'C
Cuts at: 0 1298 1425
Size: 1298 127
SfcI C'TryA_G
Cuts at: 0 628 1425
Size: 628 797
SmlI C'TyrA_G
Cuts at: 0 1021 1425
Size: 1021 404
StuI AGG'CCT
Cuts at: 0 947 1425
Size: 947 478
StyI C'CwwG_G
Cuts at: 0 227 948 1425
Size: 227 721 477
Fragments arranged by size:
721 477 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 481 844 1293 1425
Size: 481 363 449 132
Fragments arranged by size:
481 449 363 132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 969 1425
Size: 969 456
XmnI GAAnn'nnTTC
Cuts at: 0 274 1425
Size: 274 1151
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI ApaLI AvaI AvrII
BamHI BanI BanII BbsI BglI Bme1580I BmgBI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI
BsrDI BsrFI BssSI BstBI BstYI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FspI HaeII
Hin4I HincII MscI MslI MspA1I NaeI NgoMIV NspI
PfoI PmlI PpuMI PshAI PstI PvuII SacI SfcI
SmlI StuI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApoI AscI AseI AsiSI BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI Bpu10I BsiEI BsiWI BsmBI BspHI BspMI
BsrGI BssHII BstAPI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI EagI EciI EcoNI EcoRI
FalI FseI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI XmaI ZraI