(Circular) (Six-base) MAPSORT of: pFN21AA0914.seq Check: 4961 from: 1 to: 6712 pFN21AA0914 6712 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3028 3502 3028 Size: 474 6238 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4522 278 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4055 1937 Size: 2118 4594 Acc65I G'GTAC_C Cuts at: 3537 4033 3537 Size: 496 6216 AclI AA'CG_TT Cuts at: 1563 4839 5212 1563 Size: 3276 373 3063 Fragments arranged by size: 3276 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3228 3942 4775 6159 6427 1632 Size: 1596 714 833 1384 268 1917 Fragments arranged by size: 1917 1596 1384 833 714 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6515 AflIII A'CryG_T Cuts at: 1203 5611 6588 1203 Size: 4408 977 1327 Fragments arranged by size: 4408 1327 977 AgeI A'CCGG_T Cuts at: 5543 5543 Size: 6712 AhdI GACnn_n'nnGTC Cuts at: 5441 5441 Size: 6712 AleI CACnn'nnGTG Cuts at: 1166 2927 1166 Size: 1761 4951 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6712 AlwNI CAG_nnn'CTG Cuts at: 2207 2848 4455 6027 2207 Size: 641 1607 1572 2892 Fragments arranged by size: 2892 1607 1572 641 ApaLI G'TGCA_C Cuts at: 3663 4769 5925 3663 Size: 1106 1156 4450 Fragments arranged by size: 4450 1156 1106 ApoI r'AATT_y Cuts at: 1493 3167 4021 4247 4399 6508 1493 Size: 1674 854 226 152 2109 1697 Fragments arranged by size: 2109 1697 1674 854 226 152 AseI AT'TA_AT Cuts at: 160 1032 5266 160 Size: 872 4234 1606 Fragments arranged by size: 4234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 AvaI C'yCGr_G Cuts at: 1870 1943 3564 4037 1870 Size: 73 1621 473 4545 Fragments arranged by size: 4545 1621 473 73 BamHI G'GATC_C Cuts at: 4042 4409 4042 Size: 367 6345 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2829 3537 4033 5488 618 Size: 325 217 627 1042 708 496 1455 1842 Fragments arranged by size: 1842 1455 1042 708 627 496 325 217 BanII G_rGCy'C Cuts at: 1391 2609 4031 1391 Size: 1218 1422 4072 Fragments arranged by size: 4072 1422 1218 BbeI G_GCGC'C Cuts at: 2833 2833 Size: 6712 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 3615 3867 3909 928 Size: 971 963 753 252 42 3731 Fragments arranged by size: 3731 971 963 753 252 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3644 3678 4903 4937 1903 Size: 34 1707 34 1225 34 3678 Fragments arranged by size: 3678 1707 1225 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4604 5814 4604 Size: 1210 5502 BclI T'GATC_A Cuts at: 1548 1548 Size: 6712 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5323 136 Size: 107 122 71 631 605 3651 1525 Fragments arranged by size: 3651 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6707 6707 Size: 6712 BlpI GC'TnA_GC Cuts at: 4129 4129 Size: 6712 Bme1580I G_kGCm'C Cuts at: 1790 2709 3667 4773 5929 1790 Size: 919 958 1106 1156 2573 Fragments arranged by size: 2573 1156 1106 958 919 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6712 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2704 2755 3321 Size: 416 53 83 399 24 1287 51 566 Cuts at: 3321 4443 5401 442 Size: 1122 958 1753 Fragments arranged by size: 1753 1287 1122 958 566 416 399 83 53 51 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2199 2427 3350 3791 3989 5372 1239 Size: 960 228 923 441 198 1383 2579 Fragments arranged by size: 2579 1383 960 923 441 228 198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3296 4837 5702 6000 3296 Size: 1541 865 298 4008 Fragments arranged by size: 4008 1541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5375 882 Size: 617 3876 2219 Fragments arranged by size: 3876 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4503 493 Size: 711 3299 2702 Fragments arranged by size: 3299 2702 711 BsaBI GATnn'nnATC Cuts at: 6706 6706 Size: 6712 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2830 4519 4901 5536 Size: 53 83 186 936 1297 1689 382 635 Cuts at: 5536 275 Size: 1451 Fragments arranged by size: 1689 1451 1297 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2111 3063 3133 3171 5145 5543 5817 5964 6375 Size: 952 70 38 1974 398 274 147 411 Cuts at: 6375 2111 Size: 2448 Fragments arranged by size: 2448 1974 952 411 398 274 147 70 38 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3777 3807 3948 3978 1178 Size: 30 165 30 2374 30 141 30 3912 Fragments arranged by size: 3912 2374 165 141 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2699 2761 2868 2968 3611 1186 Size: 1513 62 107 100 643 4287 Fragments arranged by size: 4287 1513 643 107 100 62 BseYI C'CCAG_C Cuts at: 2633 2825 3024 5915 6411 2633 Size: 192 199 2891 496 2934 Fragments arranged by size: 2934 2891 496 199 192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6139 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3491 4162 4923 5951 1540 Size: 275 186 1490 671 761 1028 2301 Fragments arranged by size: 2301 1490 1028 761 671 275 186 BsiHKAI G_wGCw'C Cuts at: 2080 3667 3978 4773 4858 5929 2080 Size: 1587 311 795 85 1071 2863 Fragments arranged by size: 2863 1587 1071 795 311 85 BsmI GAATG_Cn' Cuts at: 3477 4223 4316 3477 Size: 746 93 5873 Fragments arranged by size: 5873 746 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2609 2709 3667 3978 4031 4773 Size: 399 290 529 100 958 311 53 742 Cuts at: 4773 4858 5929 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 958 742 529 399 311 290 100 85 53 BspEI T'CCGG_A Cuts at: 3063 3133 3171 6375 3063 Size: 70 38 3204 3400 Fragments arranged by size: 3400 3204 70 38 BspHI T'CATG_A Cuts at: 2461 2515 2961 4601 6331 2461 Size: 54 446 1640 1730 2842 Fragments arranged by size: 2842 1730 1640 446 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3028 3502 4067 844 Size: 2184 474 565 3489 Fragments arranged by size: 3489 2184 565 474 BsrBI CCG'CTC Cuts at: 1394 1823 3380 4599 1394 Size: 429 1557 1219 3507 Fragments arranged by size: 3507 1557 1219 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2104 3625 5207 5381 65 Size: 1183 856 1521 1582 174 1396 Fragments arranged by size: 1582 1521 1396 1183 856 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2672 3490 3540 5356 5543 1700 Size: 110 862 818 50 1816 187 2869 Fragments arranged by size: 2869 1816 862 818 187 110 50 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6712 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6712 BssSI C'ACGA_G Cuts at: 4772 5784 4772 Size: 1012 5700 BstAPI GCAn_nnn'nTGC Cuts at: 794 2228 794 Size: 1434 5278 BstBI TT'CG_AA Cuts at: 3084 3687 3838 5516 3084 Size: 603 151 1678 4280 Fragments arranged by size: 4280 1678 603 151 BstEII G'GTnAC_C Cuts at: 1184 2675 1184 Size: 1491 5221 BstXI CCAn_nnnn'nTGG Cuts at: 4009 4009 Size: 6712 BstYI r'GATC_y Cuts at: 1972 2619 3060 3555 4042 4409 4794 4811 6263 Size: 647 441 495 487 367 385 17 1452 Cuts at: 6263 6349 6361 6707 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 647 495 487 441 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3396 3396 Size: 6712 BtgI C'CryG_G Cuts at: 513 1065 2004 4008 513 Size: 552 939 2004 3217 Fragments arranged by size: 3217 2004 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5973 Fragments arranged by size: 5973 683 56 BtsI GCAGTG_nn' Cuts at: 4228 5022 5042 4228 Size: 794 20 5898 Fragments arranged by size: 5898 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6677 DraI TTT'AAA Cuts at: 4016 4016 Size: 6712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3638 5719 809 Size: 507 2322 2081 1802 Fragments arranged by size: 2322 2081 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3488 4159 5048 Size: 54 1425 50 275 18 1658 671 889 Cuts at: 5048 6677 8 Size: 1629 43 Fragments arranged by size: 1658 1629 1425 889 671 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3488 4159 1537 Size: 275 1676 671 4090 Fragments arranged by size: 4090 1676 671 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2745 3084 3204 3588 4642 1353 Size: 1392 339 120 384 1054 3423 Fragments arranged by size: 3423 1392 1054 384 339 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5299 5673 5819 387 Size: 1064 138 3710 374 146 1280 Fragments arranged by size: 3710 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2427 3228 3350 3791 3942 3989 Size: 393 567 228 801 122 441 151 47 Cuts at: 3989 4775 5372 6159 6427 1239 Size: 786 597 787 268 1524 Fragments arranged by size: 1524 801 787 786 597 567 441 393 268 228 151 122 47 EcoO109I rG'GnC_Cy Cuts at: 2988 3010 3214 2988 Size: 22 204 6486 Fragments arranged by size: 6486 204 22 EcoRI G'AATT_C Cuts at: 4021 4399 4021 Size: 378 6334 EcoRV GAT'ATC Cuts at: 3204 3204 Size: 6712 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3649 3649 Size: 6712 FspI TGC'GCA Cuts at: 5218 5218 Size: 6712 HaeII r_GCGC'y Cuts at: 1678 2251 2833 5859 1678 Size: 573 582 3026 2531 Fragments arranged by size: 3026 2531 582 573 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 3587 3619 3989 Size: 32 135 32 617 32 1366 32 370 Cuts at: 3989 4021 5353 5385 5427 5459 1373 Size: 32 1332 32 42 32 2626 Fragments arranged by size: 2626 1366 1332 617 370 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4056 4302 669 Size: 988 281 2118 246 3079 Fragments arranged by size: 3079 2118 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6712 HpaI GTT'AAC Cuts at: 4302 4302 Size: 6712 KasI G'GCGC_C Cuts at: 2829 2829 Size: 6712 KpnI G_GTAC'C Cuts at: 3541 4037 3541 Size: 496 6216 MfeI C'AATT_G Cuts at: 4311 4311 Size: 6712 MluI A'CGCG_T Cuts at: 6588 6588 Size: 6712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2694 5826 6010 1305 Size: 1389 3132 184 2007 Fragments arranged by size: 3132 2007 1389 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4890 Fragments arranged by size: 4890 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 2927 3739 3914 4670 5029 5188 Size: 648 1177 584 812 175 756 359 159 Cuts at: 5188 518 Size: 2042 Fragments arranged by size: 2042 1177 812 756 648 584 359 175 159 MspA1I CmG'CkG Cuts at: 1606 2144 2782 2845 4128 4805 5953 6198 1606 Size: 538 638 63 1283 677 1148 245 2120 Fragments arranged by size: 2120 1283 1148 677 638 538 245 63 NarI GG'CG_CC Cuts at: 2830 2830 Size: 6712 NcoI C'CATG_G Cuts at: 513 1065 2004 4008 513 Size: 552 939 2004 3217 Fragments arranged by size: 3217 2004 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6712 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6712 NotI GC'GGCC_GC Cuts at: 4159 4159 Size: 6712 NspI r_CATG'y Cuts at: 1132 1229 4070 5615 1132 Size: 97 2841 1545 2229 Fragments arranged by size: 2841 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6712 PciI A'CATG_T Cuts at: 5611 5611 Size: 6712 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6712 PfoI T'CCnGG_A Cuts at: 2622 3366 3795 3807 3966 2622 Size: 744 429 12 159 5368 Fragments arranged by size: 5368 744 429 159 12 PmeI GTTT'AAAC Cuts at: 4016 4016 Size: 6712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2780 2812 3250 3282 4781 4813 6294 6326 2780 Size: 32 438 32 1499 32 1481 32 3166 Fragments arranged by size: 3166 1499 1481 438 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2988 3010 3214 2988 Size: 22 204 6486 Fragments arranged by size: 6486 204 22 PshAI GACnn'nnGTC Cuts at: 1613 2056 1613 Size: 443 6269 PsiI TTA'TAA Cuts at: 4282 4282 Size: 6712 PstI C_TGCA'G Cuts at: 830 3453 4064 830 Size: 2623 611 3478 Fragments arranged by size: 3478 2623 611 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6712 PvuII CAG'CTG Cuts at: 2144 2845 2144 Size: 701 6011 SalI G'TCGA_C Cuts at: 1936 4054 1936 Size: 2118 4594 SapI GCTCTTCn'nnn_ Cuts at: 2745 2745 Size: 6712 SbfI CC_TGCA'GG Cuts at: 4064 4064 Size: 6712 ScaI AGT'ACT Cuts at: 2443 4960 6672 2443 Size: 2517 1712 2483 Fragments arranged by size: 2517 2483 1712 SfcI C'TryA_G Cuts at: 826 1045 2145 3449 4060 5195 5876 6067 826 Size: 219 1100 1304 611 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 1100 681 611 219 191 SfoI GGC'GCC Cuts at: 2831 2831 Size: 6712 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6712 SmaI CCC'GGG Cuts at: 1872 4039 1872 Size: 2167 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3275 4816 5717 5979 820 Size: 197 926 1332 1541 901 262 1553 Fragments arranged by size: 1553 1541 1332 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4503 493 Size: 4010 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6712 SphI G_CATG'C Cuts at: 1132 4070 1132 Size: 2938 3774 SspI AAT'ATT Cuts at: 5 52 4636 5 Size: 47 4584 2081 Fragments arranged by size: 4584 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6712 StyI C'CwwG_G Cuts at: 513 1065 2004 2262 4008 4151 513 Size: 552 939 258 1746 143 3074 Fragments arranged by size: 3074 1746 939 552 258 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4739 4756 4909 5094 5539 325 Size: 1255 3159 17 153 185 445 1498 Fragments arranged by size: 3159 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2441 3099 4958 Size: 274 80 33 51 1443 464 658 1859 Cuts at: 4958 6670 96 Size: 1712 138 Fragments arranged by size: 1859 1712 1443 658 464 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6672 6704 6672 Size: 32 6680 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6712 XbaI T'CTAG_A Cuts at: 4048 4048 Size: 6712 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6712 XmaI C'CCGG_G Cuts at: 1870 4037 1870 Size: 2167 4545 XmnI GAAnn'nnTTC Cuts at: 3351 3477 4841 3351 Size: 126 1364 5222 Fragments arranged by size: 5222 1364 126 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4520 276 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI