Restriction map for
pFN21AA0900
(Circular) (Six-base) MAPSORT of: pFN21AA0900.seq Check: 8255 from: 1 to: 6211
pFN21AA0900 6211 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3554 1937
Size: 1617 4594
Acc65I G'GTAC_C
Cuts at: 3532 3532
Size: 6211
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 2169 2468 4274 5658 5926 1632
Size: 450 87 299 1806 1384 268 1917
Fragments arranged by size:
1917 1806 1384 450 299 268 87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 5110 6087 1203
Size: 3907 977 1327
Fragments arranged by size:
3907 1327 977
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3315 1184
Size: 2131 4080
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 6179
AlwNI CAG_nnn'CTG
Cuts at: 3278 3954 5526 3278
Size: 676 1572 3963
Fragments arranged by size:
3963 1572 676
ApaI G_GGCC'C
Cuts at: 3203 3203
Size: 6211
ApaLI G'TGCA_C
Cuts at: 4268 5424 4268
Size: 1156 5055
ApoI r'AATT_y
Cuts at: 1493 2203 3357 3520 3746 3898 6007 1493
Size: 710 1154 163 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 710 226 163 152
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 3196 3233 3536 1870
Size: 73 1253 37 303 4545
Fragments arranged by size:
4545 1253 303 73 37
BamHI G'GATC_C
Cuts at: 2699 3541 3908 2699
Size: 842 367 5002
Fragments arranged by size:
5002 842 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2564 2607 3532 4987 618
Size: 325 217 627 777 43 925 1455 1842
Fragments arranged by size:
1842 1455 925 777 627 325 217 43
BanII G_rGCy'C
Cuts at: 1391 2034 2056 3174 3203 3530 1391
Size: 643 22 1118 29 327 4072
Fragments arranged by size:
4072 1118 643 327 29 22
BbeI G_GCGC'C
Cuts at: 2611 2611
Size: 6211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2157 2520 2682 3231 928
Size: 971 258 363 162 549 3908
Fragments arranged by size:
3908 971 549 363 258 162
BbvCI CC'TCA_GC
Cuts at: 2907 3064 3279 2907
Size: 157 215 5839
Fragments arranged by size:
5839 215 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4402 4436 1903
Size: 34 50 34 2381 34 3678
Fragments arranged by size:
3678 2381 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BfrBI ATG'CAT
Cuts at: 2729 3262 2729
Size: 533 5678
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4822 136
Size: 107 122 71 631 605 3150 1525
Fragments arranged by size:
3150 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 2126 3628 2126
Size: 1502 4709
Bme1580I G_kGCm'C
Cuts at: 1790 3203 4272 5428 1790
Size: 1413 1069 1156 2573
Fragments arranged by size:
2573 1413 1156 1069
BmgBI CAC'GTC
Cuts at: 1318 2818 3424 1318
Size: 1500 606 4105
Fragments arranged by size:
4105 1500 606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3266 3942 4900
Size: 416 53 83 399 24 1849 676 958
Cuts at: 4900 442
Size: 1753
Fragments arranged by size:
1849 1753 958 676 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2958 3077 4871 1239
Size: 1719 119 1794 2579
Fragments arranged by size:
2579 1794 1719 119
Bpu10I CC'TnA_GC
Cuts at: 2907 3064 3279 2907
Size: 157 215 5839
Fragments arranged by size:
5839 215 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4336 5201 5499 4336
Size: 865 298 5048
Fragments arranged by size:
5048 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2278 4874 882
Size: 617 779 2596 2219
Fragments arranged by size:
2596 2219 779 617
BsaAI yAC'GTr
Cuts at: 493 1204 4002 493
Size: 711 2798 2702
Fragments arranged by size:
2798 2702 711
BsaBI GATnn'nnATC
Cuts at: 6205 6205
Size: 6211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2536 2608 4018 4400
Size: 53 83 186 936 1003 72 1410 382
Cuts at: 4400 5035 275
Size: 635 1451
Fragments arranged by size:
1451 1410 1003 936 635 382 186 83
72 53
BsaWI w'CCGG_w
Cuts at: 3026 4644 5042 5316 5463 5874 3026
Size: 1618 398 274 147 411 3363
Fragments arranged by size:
3363 1618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2843 2873 3146 3176 1178
Size: 30 165 30 1440 30 273 30 4213
Fragments arranged by size:
4213 1440 273 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3027 3039 3138 3141 3326 1186
Size: 1841 12 99 3 185 4071
Fragments arranged by size:
4071 1841 185 99 12 3
BseYI C'CCAG_C
Cuts at: 2247 2784 2995 5414 5910 2247
Size: 537 211 2419 496 2548
Fragments arranged by size:
2548 2419 537 496 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2762 2946 3285 3395 1163
Size: 573 1026 184 339 110 3979
Fragments arranged by size:
3979 1026 573 339 184 110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2056 4272 4357 5428 2056
Size: 2216 85 1071 2839
Fragments arranged by size:
2839 2216 1071 85
BsmI GAATG_Cn'
Cuts at: 2352 3722 3815 2352
Size: 1370 93 4748
Fragments arranged by size:
4748 1370 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2056 3174 3203 3530 4272 4357
Size: 399 244 22 1118 29 327 742 85
Cuts at: 4357 5428 1391
Size: 1071 2174
Fragments arranged by size:
2174 1118 1071 742 399 327 244 85
29 22
BspEI T'CCGG_A
Cuts at: 3026 5874 3026
Size: 2848 3363
BspHI T'CATG_A
Cuts at: 4100 5830 4100
Size: 1730 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3566 844
Size: 2722 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3447 4706 4880 65
Size: 1183 2199 1259 174 1396
Fragments arranged by size:
2199 1396 1259 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2862 4855 5042 1700
Size: 110 1052 1993 187 2869
Fragments arranged by size:
2869 1993 1052 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3074 794
Size: 2280 3931
BstBI TT'CG_AA
Cuts at: 3349 5015 3349
Size: 1666 4545
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstYI r'GATC_y
Cuts at: 1972 2699 3541 3908 4293 4310 5762 5848 5860
Size: 727 842 367 385 17 1452 86 12
Cuts at: 5860 6206 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 842 727 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2934 2934
Size: 6211
BtgI C'CryG_G
Cuts at: 513 1065 2680 3453 513
Size: 552 1615 773 3271
Fragments arranged by size:
3271 1615 773 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5472
Fragments arranged by size:
5472 683 56
BtsI GCAGTG_nn'
Cuts at: 3727 4521 4541 3727
Size: 794 20 5397
Fragments arranged by size:
5397 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6176
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3426 3658 4547
Size: 54 1425 50 275 18 1596 232 889
Cuts at: 4547 6176 8
Size: 1629 43
Fragments arranged by size:
1629 1596 1425 889 275 232 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2214 2262 4141 1353
Size: 659 202 48 1879 3423
Fragments arranged by size:
3423 1879 659 202 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3030 4798 5172 5318 387
Size: 1064 138 1441 1768 374 146 1280
Fragments arranged by size:
1768 1441 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2054 2054
Size: 6211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2169 2468 2958 3077 4274 4871
Size: 393 450 87 299 490 119 1197 597
Cuts at: 4871 5658 5926 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1197 787 597 490 450 393 299
268 119 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2881 2881
Size: 6211
EcoO109I rG'GnC_Cy
Cuts at: 2938 2949 3221 2938
Size: 11 272 5928
Fragments arranged by size:
5928 272 11
EcoRI G'AATT_C
Cuts at: 3357 3520 3898 3357
Size: 163 378 5670
Fragments arranged by size:
5670 378 163
EcoRV GAT'ATC
Cuts at: 2287 2287
Size: 6211
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 2611 5358 1678
Size: 933 2747 2531
Fragments arranged by size:
2747 2531 933
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2705 2737 3464 3496 4852
Size: 32 135 32 1133 32 727 32 1356
Cuts at: 4852 4884 4926 4958 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 1133 727 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3555 3801 669
Size: 988 281 1617 246 3079
Fragments arranged by size:
3079 1617 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2301 748
Size: 1553 4658
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KasI G'GCGC_C
Cuts at: 2607 2607
Size: 6211
KpnI G_GTAC'C
Cuts at: 3536 3536
Size: 6211
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3531 5325 5509 1305
Size: 2226 1794 184 2007
Fragments arranged by size:
2226 2007 1794 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3428 10
Size: 54 1425 343 1596 2793
Fragments arranged by size:
2793 1596 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3452 4169 4528 4687 518
Size: 648 2286 717 359 159 2042
Fragments arranged by size:
2286 2042 717 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 2251 2810 3385 3627 4304 5452 5697
Size: 519 126 559 575 242 677 1148 245
Cuts at: 5697 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 575 559 519 245 242
126
NarI GG'CG_CC
Cuts at: 2608 2608
Size: 6211
NcoI C'CATG_G
Cuts at: 513 1065 2680 513
Size: 552 1615 4044
Fragments arranged by size:
4044 1615 552
NdeI CA'TA_TG
Cuts at: 387 2632 387
Size: 2245 3966
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NsiI A_TGCA'T
Cuts at: 2731 3264 2731
Size: 533 5678
NspI r_CATG'y
Cuts at: 1132 1229 2147 2729 3569 5114 1132
Size: 97 918 582 840 1545 2229
Fragments arranged by size:
2229 1545 918 840 582 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6211
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 3263 1108
Size: 1227 928 4056
Fragments arranged by size:
4056 1227 928
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2949 2949
Size: 6211
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PspOMI G'GGCC_C
Cuts at: 3199 3199
Size: 6211
PstI C_TGCA'G
Cuts at: 830 3563 830
Size: 2733 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2125 2251 2810 2125
Size: 126 559 5526
Fragments arranged by size:
5526 559 126
SacI G_AGCT'C
Cuts at: 2056 2056
Size: 6211
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SanDI GG'GwC_CC
Cuts at: 2949 2949
Size: 6211
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 2101 4459 6171 2101
Size: 2358 1712 2141
Fragments arranged by size:
2358 2141 1712
SfcI C'TryA_G
Cuts at: 826 1045 2279 2501 3559 4694 5375 5566 826
Size: 219 1234 222 1058 1135 681 191 1471
Fragments arranged by size:
1471 1234 1135 1058 681 222 219 191
SfoI GGC'GCC
Cuts at: 2609 2609
Size: 6211
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3198 3235 3538 1872
Size: 1326 37 303 4545
Fragments arranged by size:
4545 1326 303 37
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4315 5216 5478 820
Size: 197 926 2372 901 262 1553
Fragments arranged by size:
2372 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 4135 5
Size: 47 4083 2081
Fragments arranged by size:
4083 2081 47
StuI AGG'CCT
Cuts at: 1517 2957 1517
Size: 1440 4771
StyI C'CwwG_G
Cuts at: 513 1065 2680 3650 513
Size: 552 1615 970 3074
Fragments arranged by size:
3074 1615 970 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 3171 4238 4255 4408 4593 5038
Size: 1255 681 910 1067 17 153 185 445
Cuts at: 5038 325
Size: 1498
Fragments arranged by size:
1498 1255 1067 910 681 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2099 3459 4457
Size: 274 80 33 51 1443 122 1360 998
Cuts at: 4457 6169 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1360 998 274 138 122 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 6171 6203 3146
Size: 32 2993 32 3154
Fragments arranged by size:
3154 2993 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3172 3172
Size: 6211
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 3196 3233 3536 1870
Size: 1326 37 303 4545
Fragments arranged by size:
4545 1326 303 37
XmnI GAAnn'nnTTC
Cuts at: 4340 4340
Size: 6211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BplI BsiWI BsmBI
BstXI BstZ17I DraIII FalI FseI FspAI NaeI NgoMIV
NruI PacI PfoI PmlI PspXI PsrI RsrII SacII
SapI SexAI SfiI SrfI SwaI